Did you mean: edwardsiella hoshina
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International Journal of Molecular... Mar 2023The genus presents five different pathogenic species: , , , and . These species cause infections mainly in fish, but they can also infect reptiles, birds or humans....
The genus presents five different pathogenic species: , , , and . These species cause infections mainly in fish, but they can also infect reptiles, birds or humans. Lipopolysaccharide (endotoxin) plays an important role in the pathogenesis of these bacteria. For the first time, the chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharides of , , and were studied. The complete gene assignments for all core biosynthesis gene functions were acquired. The structure of core oligosaccharides was investigated by ¹H and C nuclear magnetic resonance (NMR) spectroscopy. The structures of and core oligosaccharides show the presence of →3,4)-L--α-D--Hep, two terminal β-D-Glc, →2,3,7)-L--α-D--Hep, →7)-L--α-D--Hep, terminal α-D-GlcN, two →4)-α-D-GalA, → 3)-α-D-GlcNAc, terminal β-D-Gal and →5-substituted Kdo. core oligosaccharide shows only one terminal β-D-Glc, and instead of terminal β-D-Gal a terminal α-D-GlcNAc. core oligosaccharide shows only one terminal β-D-Glc, one →4)-α-D-GalA and do not have terminal α-D-GlcN (see complementary figure).
Topics: Animals; Humans; Carbohydrate Sequence; Lipopolysaccharides; Oligosaccharides; Magnetic Resonance Spectroscopy; Methylation
PubMed: 36902212
DOI: 10.3390/ijms24054768 -
Antimicrobial Agents and Chemotherapy Aug 2001The natural antibiotic susceptibilities to 71 antibiotics of 102 Edwardsiella strains belonging to E. tarda (n = 42), E. ictaluri (n = 41), and E. hoshinae (n = 19) were... (Comparative Study)
Comparative Study
The natural antibiotic susceptibilities to 71 antibiotics of 102 Edwardsiella strains belonging to E. tarda (n = 42), E. ictaluri (n = 41), and E. hoshinae (n = 19) were investigated. MICs were determined using a microdilution procedure according to NCCLS criteria and German standards. All edwardsiellae were naturally sensitive to tetracyclines, aminoglycosides, most beta-lactams, quinolones, antifolates, chloramphenicol, nitrofurantoin, and fosfomycin. Edwardsiella species were naturally resistant to macrolides, lincosamides, streptogramins, glycopeptides, rifampin, fusidic acid, and oxacillin. Although slight species-dependent differences in natural susceptibilities to some antibiotics (e.g., macrolides and cefaclor) were seen, differences in natural susceptibility affecting clinical assessment criteria were only seen with benzylpenicillin. Whereas E. tarda was naturally resistant to benzylpenicillin, E. hoshinae was naturally sensitive. Natural sensitivity and resistance to this penicillin were found among the strains of E. ictaluri. The observed oxacillin sensitivity of E. ictaluri was attributed to the failure of the species to grow at higher salt concentrations found in oxacillin-containing microtiter plates. The present study describes a database concerning the natural susceptibility of Edwardsiella species to a wide range of antibiotics, which can be applied to validate forthcoming antibiotic susceptibility tests of these microorganisms.
Topics: Anti-Bacterial Agents; Databases, Factual; Drug Resistance, Microbial; Edwardsiella ictaluri; Edwardsiella tarda; Humans; Microbial Sensitivity Tests
PubMed: 11451681
DOI: 10.1128/AAC.45.8.2245-2255.2001 -
Genome Announcements Feb 2017is a Gram-negative facultative anaerobe that has primarily been isolated from avians and reptiles. We report here the complete and annotated genome sequence of an...
is a Gram-negative facultative anaerobe that has primarily been isolated from avians and reptiles. We report here the complete and annotated genome sequence of an isolate from a monitor lizard ( sp.), which contains a chromosome of 3,811,650 bp and no plasmids.
PubMed: 28183769
DOI: 10.1128/genomeA.01605-16 -
Journal of Clinical Microbiology Sep 1991The pathogenic characteristics of 35 Edwardsiella strains from clinical and environmental sources were investigated. Overall, most Edwardsiella tarda strains were...
The pathogenic characteristics of 35 Edwardsiella strains from clinical and environmental sources were investigated. Overall, most Edwardsiella tarda strains were invasive in HEp-2 cell monolayers, produced a cell-associated hemolysin and siderophores, and bound Congo red; many strains also expressed mannose-resistant hemagglutination against guinea pig erythrocytes. Edwardsiella hoshinae strains bound Congo red and were variable in their invasive and hemolytic capabilities while Edwardsiella ictaluri strains did not produce either factor; neither E. hoshinae nor E. ictaluri expressed mannose-resistant hemagglutination nor elaborated siderophores under the tested conditions. Selected strains of each species tested for mouse lethality indicated strain variability in pathogenic potential, with E. tarda strains being the most virulent; 50% lethal doses in individual strains did not correlate with plasmid content, chemotactic motility, serum resistance, or expression of selected enzyme activities. The results suggest some potential important differences in pathogenic properties that may help explain their environmental distribution and ability to cause disease in humans.
Topics: Animals; Biomarkers; Enterobacteriaceae; Enterobacteriaceae Infections; Female; Hemolysin Proteins; Humans; Mice; Phenotype; Plasmids; Species Specificity; Virulence
PubMed: 1774326
DOI: 10.1128/jcm.29.9.1997-2001.1991 -
International Journal of Microbiology 2013From 194 faecal dropping samples of common house geckos collected from offices (60), houses (88), integrated farm units (IFS,18) and hostels, guest houses, and dining...
From 194 faecal dropping samples of common house geckos collected from offices (60), houses (88), integrated farm units (IFS,18) and hostels, guest houses, and dining rooms of different canteen/mess (HGM, 28), 326 bacterial isolates of enteric bacteria belonging to 17 genera and 34 species were detected. Escherichia coli were the most frequently (39) isolated followed by Citrobacter freundii (33), Klebsiella pneumonia (27), Salmonella indica (12), Enterobacter gergoviae (12), and Ent. agglomerans (11). Other important bacteria isolated from gecko droppings were Listonella damsela (2), Raoultella terrigena (3), S. salamae (2), S. houtenae (3), Edwardsiella tarda (4), Edwardsiella hoshinae (1), and Klebsiella oxytoca (2). Of the 223 isolates tested for antimicrobial drug sensitivity, 27 (12.1%) had multiple drug resistance (MDR). None of the salmonellae or edwardsiellae had MDR however, MDR strains were significantly more common among Escherichia spp. (P = 1.9 × 10(-5)) and isolates from IFS units (P = 3.58 × 10(-23)). The most effective herbal drug, Ageratum conyzoides extract, inhibited growth of only 27.8% of strains tested followed by ethanolic extract of Zanthoxylum rhetsa (13.9%), eucalyptus oil (5.4%), patchouli oil (5.4%), lemongrass oil (3.6%), and sandalwood oil (3.1%), and Artemisia vulgaris essential oil (3.1%).
PubMed: 24223595
DOI: 10.1155/2013/340848 -
Infection and Immunity Jan 1991The ability of 22 Edwardsiella strains to penetrate and replicate in cultured epithelial cells was initially evaluated by light microscopy methods and by the recovery of...
The ability of 22 Edwardsiella strains to penetrate and replicate in cultured epithelial cells was initially evaluated by light microscopy methods and by the recovery of gentamicin-resistant (Gmr) bacteria from the Triton X-100 cell lysates of HEp-2-infected monolayers. Giemsa-stained HEp-2 cells revealed the presence of numerous internalized bacteria 3 h postinfection, often appearing as parallel rows of replicated bacteria within the cytosol and sometimes obliterating the cytoplasm because of the large numbers of bacilli present. Invasive bacteria were also sometimes found within cytoplasmic vacuoles in infected cells; thin-section electron micrographs of HEp-2-infected cells supported these conclusions. Results of light microscopy studies and cell lysate assays indicated that most Edwardsiella tarda (92%) and some Edwardsiella hoshinae strains were invasion positive on one or more occasions, while Edwardsiella ictaluri isolates were uniformly negative. HEp-2 invasion by E. tarda was a microfilament-dependent (cytochalasin B- and D-sensitive) process, with maximum numbers of Gmr CFU recorded between 3 and 6 h postinfection. The small percentage (0.01 to 1.0%) of the challenge inoculum recoverable as Gmr progeny 3 to 6 h postinfection was attributed to a strong cell-associated (not filterable) hemolysin that was produced by a majority (85%) of the E. tarda strains but not by E. ictaluri and only minimally by E. hoshinae. This cytolysin/hemolysin was responsible for the toxic effects observed in HEp-2 cells during the infection-replication process of edwardsiellae and appears to play a role in the release of internalized and replicated bacteria from infected cells. The results suggest an invasion strategy with some similarities to and differences from those of other recognized enteroinvasive pathogens.
Topics: Cells, Cultured; Enterobacteriaceae; Epithelium; Hemolysin Proteins; Shigella; Virulence
PubMed: 1987028
DOI: 10.1128/iai.59.1.154-161.1991 -
Frontiers in Microbiology 2019-associated outbreaks are increasingly reported on both marine and freshwater aquaculture setups, accounting for severe financial and biomass losses. , and have been...
-associated outbreaks are increasingly reported on both marine and freshwater aquaculture setups, accounting for severe financial and biomass losses. , and have been the traditional causative agents of edwardsiellosis in aquaculture, however, intensive studies due to the significance of the disease have just recently revealed two more species, and . Whole genome sequencing that was conducted on the strain EA011113, isolated from farmed after an edwardsiellosis outbreak in Greece, confirmed it as a new clinical strain of . Extensive phylogenetic analysis showed that this Greek strain is closely related to an Israeli like clinical strain, isolated from diseased groupers, and in Red Sea. Bioinformatic analyses of strain EA011113 unveiled a wide repertoire of potential virulence factors, the effect of which was corroborated by the mortalities that the strain induced in adult zebrafish, , under different levels of infection intensity (LD after 48 h: 1.85 × 10 cfu/fish). This strain was non-motile and according to electron microscopy lacked flagella, a fact that is not typical for . Comparative genomic analysis revealed a deletion of 36 nt found in the flagellar biosynthetic gene () that could explain that trait. Further analysis revealed an intact prophage that was integrated in the bacterial genome. Following spontaneous induction, the phage was isolated, purified, characterized and independently sequenced, confirming its viability as a free, inducible virion as well. Separate genomic analysis of the prophage implies a plausible case of lysogenic conversion. Focusing on edwardsiellosis as a rapidly emerging aquaculture disease on a global scale, this work offers some insight into the virulence, fitness, and potential lysogenic conversion of a of a newly described, yet highly pathogenic, strain of .
PubMed: 30787917
DOI: 10.3389/fmicb.2019.00141 -
Journal of Infection and Public Health 2017The emergence and spread of carbapenem-resistant bacteria are a significant clinical and public health concern. The aim of the study is to determine the prevalence of...
Prevalence, antibiotic susceptibility and characterization of antibiotic resistant genes among carbapenem-resistant Gram-negative bacilli and yeast in intestinal flora of cancer patients in North Lebanon.
The emergence and spread of carbapenem-resistant bacteria are a significant clinical and public health concern. The aim of the study is to determine the prevalence of intestinal carriage of carbapenem-resistant bacteria and yeasts in cancer patients under chemotherapy. 41 stool samples collected from cancer patients in Nini hospital in Tripoli, North Lebanon have been analyzed. After isolating yeasts and carbapenem-resistant bacteria, a biochemical identification and antimicrobial susceptibility profile were determined. The mechanism of enzymatic carbapenem-resistance was detected by searching for carbapenemases by both Hodge test and PCR assays. The association of several mechanisms of resistance was also searched. 46.3% (19/41) of patients were colonized by yeast. Candida glabrata (6/19) was the major species. The prevalence of carbapenem-resistant bacteria was 24.4% (10/41) including Escherichia coli (5/10), Enterobacter cloacae (1/10), Enterobacter aerogenes (1/10) Edwardsiella hoshinae (1/10) Pantoea agglomerans (1/10) and Pseudomonas stutzeri (1/10). PCR and sequencing of the amplified fragments revealed that Pseudomonas stutzeri (1/1) carried VIM gene and Enterobacter aerogenes (1/1) and E. coli (1/5) carried OXA-48 gene. The other Enterobacteriaceae were resistant to carbapenems by mechanisms other than a carbapenemase including hyperproduction of cephalosporinase (4/10), extended spectrum beta-lactamases (1/10) and both cephalosporinase and extended spectrum beta-lactamases (2/10). High prevalence of intestinal carriage of carbapenem-resistant bacteria and yeasts were detected in cancer patients under chemotherapy. In order to prevent the development of endogenous infection and the dissemination of antimicrobial resistance, an implementation of antibiotic stewardship programs and infection control measures is required in hospitals particularly in the department of chemotherapy.
Topics: Bacterial Proteins; Drug Resistance, Bacterial; Drug Resistance, Fungal; Dysbiosis; Gastrointestinal Microbiome; Gram-Negative Bacteria; Humans; Lebanon; Microbial Sensitivity Tests; Neoplasms; Polymerase Chain Reaction; Prevalence; Sequence Analysis, DNA; Surveys and Questionnaires; Yeasts; beta-Lactamases
PubMed: 28216021
DOI: 10.1016/j.jiph.2016.10.009 -
The Malaysian Journal of Pathology Jun 2006Edwardsiella tarda has recently been described as a member of the family Enterobacteriaceae. The genus Edwardsiella contains three species; E. hoshinae, E. ictaluri and...
Edwardsiella tarda has recently been described as a member of the family Enterobacteriaceae. The genus Edwardsiella contains three species; E. hoshinae, E. ictaluri and E. tarda. Edwardsiella tarda is the only species which has been recognised as pathogenic to humans, especially in those with an underlying disease. The most common presentation is watery diarrhoea. Extra intestinal infections have been reported infrequently. Humans seem to be infected or colonised with Edwardsiella through ingestion or inoculation of a wound. This report is of a patient with multiple liver abscesses due to E. tarda who later developed bacterial peritonitis and septicaemic shock.
Topics: Adult; Anti-Bacterial Agents; Edwardsiella tarda; Enterobacteriaceae Infections; Fatal Outcome; Humans; Liver Abscess; Male; Peritonitis; Sepsis; Suction; Tomography, X-Ray Computed
PubMed: 17694959
DOI: No ID Found