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International Journal of Systematic and... Jun 2015A Gram-stain-positive, endospore-forming, moderately halophilic bacterial strain, NEAU-ST10-40T, was isolated from a saline and alkaline soil in Anda City, China. It was...
A Gram-stain-positive, endospore-forming, moderately halophilic bacterial strain, NEAU-ST10-40T, was isolated from a saline and alkaline soil in Anda City, China. It was strictly aerobic, rod-shaped and motile by peritrichous flagella. It formed light yellow colonies and grew at NaCl concentrations of 3-15 % (w/v) (optimum, 8 %, w/v), at pH 7.0-9.0 (optimum, pH 8.0) and at 4-60 °C (optimum, 30 °C). It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it belonged to the genus Halobacillus. Levels of 16S rRNA gene sequence similarity between strain NEAU-ST10-40T and the type strains of related species of the genus Halobacillus ranged from 98.8 % (Halobacillus alkaliphilus FP5T) to 97.1 % (Halobacillus kuroshimensis IS-Hb7T). DNA-DNA hybridization relatedness values between strain NEAU-ST10-40T and H. alkaliphilus DSM 18525T, Halobacillus campisalis KCTC 13144T, Halobacillus yeomjeoni DSM 17110T, Halobacillus halophilus DSM 2266T, Halobacillus litoralis DSM 10405T, Halobacillus dabanensis DSM 18199T, Halobacillus salinus DSM 18897T, Halobacillus naozhouensis DSM 21183T, Halobacillus trueperi DSM 10404T and Halobacillus salsuginis DSM 21185T were from 43 ± 1 to 19 ± 1 % (mean ± sd). The DNA G+C content was 39.3 mol%. The major fatty acids (>10 %) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0, the only respiratory quinone detected was MK-7, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and three unknown lipids. On the basis of the data presented, strain NEAU-ST10-40T is considered to represent a novel species, for which the name Halobacillus andaensis sp. nov. is proposed. The type strain is NEAU-ST10-40T ( = CGMCC 1.12153T = DSM 25866T).
Topics: Base Composition; China; DNA, Bacterial; Diaminopimelic Acid; Fatty Acids; Halobacillus; Hydrogen-Ion Concentration; Molecular Sequence Data; Nucleic Acid Hybridization; Peptidoglycan; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Salinity; Sequence Analysis, DNA; Soil; Soil Microbiology; Vitamin K 2
PubMed: 25795064
DOI: 10.1099/ijs.0.000198 -
International Journal of Systematic and... Oct 2023Three bacterial strains, designated SSBR10-3, SSTM10-2 and SSHM10-5, were isolated from saltern soil sampled in Jeollanam-do, Republic of Korea. Cells were aerobic,...
Three bacterial strains, designated SSBR10-3, SSTM10-2 and SSHM10-5, were isolated from saltern soil sampled in Jeollanam-do, Republic of Korea. Cells were aerobic, Gram-stain-positive, flagellated and rod-shaped. The strains grew optimally at 28°C and at pH 7.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains SSBR10-3, SSTM10-2 and SSHM10-5 were placed within the genus , showing the highest similarity to FP5 (98.6 %), '' Marseille-Q1234 (98.5 %) and MSS-155 (98.6 %), respectively. The genomic similarity values between strains SSBR10-3, SSTM10-2 and SSHM10-5 and their related species were 17.6-22.6 % for digital DNA-DNA hybridization (dDDH) and 69.6-78.5 % for orthologous average nucleotide identity (OrthoANI), which were lower than the thresholds recommended for species delineation. The dDDH and OrthoANI values among the three strains were below 38.3 and 89.4 %, respectively. Besides the differences in genomic features, strains SSBR10-3, SSTM10-2 and SSHM10-5 were distinct from each other and from members of the genus in terms of phenotypic traits related to substrate assimilation. The cell-wall peptidoglycan contained -diaminopimelic acid, the major fatty acids were anteiso-C, iso-C and anteiso-C, and the predominant menaquinone was MK-7 for all three strains. Diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid were present in their polar lipid profiles. Based on a polyphasic approach incorporating genomic data, strains SSBR10-3, SSTM10-2 and SSHM10-5 represent novel species, for which the names sp. nov. (SSBR10-3=DSM 114353=KACC 21935=NBRC 115504), sp. nov. (SSTM10-2=DSM 114354=KACC 21936=NBRC 115505) and sp. nov. (SSHM10-5=DSM 114355= KACC 21937=NBRC 115506) are proposed.
Topics: Fatty Acids; Halobacillus; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; DNA, Bacterial; Bacterial Typing Techniques; Base Composition; Nucleotides
PubMed: 37882660
DOI: 10.1099/ijsem.0.006098 -
International Journal of Systematic and... Nov 2021An aerobic, Gram-stain-positive, endospore-forming, rod-shaped and moderately halophilic strain SKP4-6, was isolated from shrimp paste () collected from Samut Sakhon...
An aerobic, Gram-stain-positive, endospore-forming, rod-shaped and moderately halophilic strain SKP4-6, was isolated from shrimp paste () collected from Samut Sakhon Province, Thailand. Phylogenetic analysis revealed that strain SKP4-6 belonged to the genus and was most closely related to JCM 11546 (98.6 %), KCTC 3788 (98.6 %) and KCTC 3957 (98.6 %) based on 16S rRNA gene sequence similarity. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain SKP4-6 and its related species were 18.2-19.3 % and 69.84-84.51 %, respectively, which were lower than the threshold recommended for species delineation. The strain grew optimally at 30-40 °C, at pH 7.0 and with 10-15 % (w/v) NaCl. It contained l-Orn-d-Asp in the cell wall peptidoglycan. The DNA G+C content was 44.8 mol%. The major fatty acids were iso-C, anteiso-C and anteiso-C. The predominant isoprenoid quinone was MK-7. Phosphatidylglycerol and diphosphatidylglycerol were present as major polar lipids. Based on this polyphasic approach, digital DNA-DNA relatedness and ANI values, strain SKP4-6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SKP4-6 (=JCM 32624=TISTR 2595).
Topics: Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Food Microbiology; Halobacillus; Nucleic Acid Hybridization; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Seafood; Sequence Analysis, DNA; Thailand; Vitamin K 2
PubMed: 34825883
DOI: 10.1099/ijsem.0.005054 -
Microbiology Resource Announcements Nov 2023We present the whole-genome sequence of Korean Agricultural Culture Collection (KACC) 21980, isolated from China by Chen et al.. The genome of KACC 21980 comprises a...
We present the whole-genome sequence of Korean Agricultural Culture Collection (KACC) 21980, isolated from China by Chen et al.. The genome of KACC 21980 comprises a circular chromosome (4.2 Mb) and one plasmid (17 kb). It includes a total of 4,168 predicted coding genes.
PubMed: 37874147
DOI: 10.1128/MRA.00570-23 -
Bioengineered Feb 2022This study investigated the inhibitory activity of S61 and its active extract on potato dry rot pathogens and aimed at contributing to biocontrol agent development...
This study investigated the inhibitory activity of S61 and its active extract on potato dry rot pathogens and aimed at contributing to biocontrol agent development during potato storage. Three kinds of pathogens were isolated as target pathogenic fungi from dry rot tubers and determined as (Qing 9A-2), (Qing 9A-5-8) and (Qing 9A-1-1) by morphological and molecular identification. The strain S61 and its extract exhibited a higher inhibitory rate on both three pathogens (56.32-65.75 and 1.67-51.11%), notably the best suppression efficiency is presented in S61 and 40 mg/mL ethyl acetate extract. In terms of in vivo effects, both S61 and its ethyl acetate extract effectively reduced the decayed fruit and weight loss rate (0-20% and 7.59-16.56%) and enhanced the defensive enzymatic activities to improve resistance. In addition, strain S61 could be colonized on potato tubers, especially the highest amount of 1.55 × 10 CFU/mL on fifth day for variety Xiazhai 65. Overall, S61 and its ethyl acetate extract could be considered as potential approach for biocontrol potato dry rot.
Topics: Fungi; Halobacillus; Solanum tuberosum
PubMed: 35164641
DOI: 10.1080/21655979.2021.2024375 -
International Journal of Systematic and... Nov 2005A Gram-positive or Gram-variable, motile, endospore-forming, halophilic bacterial strain, MSS-402T, was isolated from a marine solar saltern in Korea, and subjected to a...
A Gram-positive or Gram-variable, motile, endospore-forming, halophilic bacterial strain, MSS-402T, was isolated from a marine solar saltern in Korea, and subjected to a polyphasic taxonomic study. Some cells of strain MSS-402T were long filamentous rods. The isolate grew optimally at 37 degrees C and in the presence of 3-5 % (w/v) NaCl. Strain MSS-402T had cell-wall peptidoglycan based on l-orn-d-Asp, MK-7 as the predominant menaquinone and anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) as major fatty acids. The DNA G+C content was 42.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MSS-402T falls within the evolutionary radiation of species of the genus Halobacillus. Levels of 16S rRNA gene sequence similarity between strain MSS-402T and the type strains of recognized Halobacillus species ranged from 98.0 % (with Halobacillus halophilus) to 99.2 % (with Halobacillus litoralis and Halobacillus trueperi). Levels of DNA-DNA binding indicated that strain MSS-402T represents a genomic species that is distinct from recognized Halobacillus species. Strain MSS-402T was differentiated from Halobacillus species by means of several phenotypic characteristics. On the basis of its phenotypic properties and phylogenetic and genetic distinctiveness, strain MSS-402T (=KCTC 3957T=DSM 17110T) should be classified as the type strain of a novel Halobacillus species, for which the name Halobacillus yeomjeoni sp. nov. is proposed.
Topics: Bacillaceae; Base Composition; Base Sequence; DNA, Bacterial; DNA, Ribosomal; Korea; Molecular Sequence Data; Phenotype; Phylogeny; Seawater; Sodium Chloride
PubMed: 16280505
DOI: 10.1099/ijs.0.63801-0 -
New Microbes and New Infections Sep 2020Strain Marseille-Q1234 is a new species from the genus that was isolated in 2019 from a stool sample in a healthy Malian child <5 years old. Cells are Gram-positive and...
Strain Marseille-Q1234 is a new species from the genus that was isolated in 2019 from a stool sample in a healthy Malian child <5 years old. Cells are Gram-positive and strictly halophilic bacilli. Strain Marseille-Q1234 exhibits 98.46% 16S rRNA gene sequence similarity to strain JSM 071068 (NR_116505.1), the phylogenetically closely related species with standing in nomenclature. Based on the phenotypic and phylogenetic evidence, OrthoANI values and results of the biochemical tests, the new species is named sp. nov., for which strain Marseille-Q1234 (= CSURQ1234) is proposed as the type strain.
PubMed: 32793353
DOI: 10.1016/j.nmni.2020.100708 -
Antonie Van Leeuwenhoek Jun 2009A moderately halophilic, Gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain JSM 071068(T), was isolated from a sea anemone...
A moderately halophilic, Gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain JSM 071068(T), was isolated from a sea anemone (Anthopleura xanthogrammica) collected from the Naozhou Island on the Leizhou Bay in the South China Sea. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 071068(T) was able to grow with 1-20% (w/v) total salts (optimum, 6-9%), at pH values of 6.0-10.0 (optimum, pH 7.5) and a temperature range of 10-35 degrees C (optimum, 25 degrees C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant menaquinone was MK-7 and the major cellular fatty acids were anteiso-C(15:0), anteiso-C(17:0) and iso-C(15:0). The genomic DNA G + C content was 42.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 071068(T) belonged to the genus Halobacillus. The 16S rRNA gene sequence similarities between strain JSM 071068(T) and the type strains of the recognized Halobacillus species ranged from 97.9% (with Halobacillus alkaliphilus) to 95.3% (with Halobacillus kuroshimensis). The levels of DNA-DNA relatedness between the new isolate and the type strains of H. alkaliphilus, Halobacillus campisalis, Halobacillus halophilus and Halobacillus seohaensis were 25.6, 22.1, 10.8 and 13.2%, respectively. The combination of phylogenetic analysis, DNA-DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 071068(T) represents a new species of the genus Halobacillus, for which the name Halobacillus naozhouensis sp. nov. is proposed, with JSM 071068(T) (=DSM 21183(T) =KCTC 13234(T)) as the type strain.
Topics: Animals; Bacillaceae; Base Composition; Cell Wall; China; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Flagella; Hydrogen-Ion Concentration; Locomotion; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S; Sea Anemones; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Sodium Chloride; Spores, Bacterial; Temperature; Vitamin K 2
PubMed: 19370398
DOI: 10.1007/s10482-009-9340-9 -
Antonie Van Leeuwenhoek May 2016A Gram-positive, moderately halophilic bacterium, designated strain TGS-15(T), was isolated from the sediment of a solar saltern pond located in Shinan, Korea. Strain...
A Gram-positive, moderately halophilic bacterium, designated strain TGS-15(T), was isolated from the sediment of a solar saltern pond located in Shinan, Korea. Strain TGS-15(T) was found to be a strictly aerobic, non-motile rod which can grow at pH 6.0-10.0 (optimum, pH 9.0), at 20-35 °C (optimum, 28 °C) and at salinities of 1-20 % (w/v) NaCl (optimum, 9 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TGS-15(T) belongs to the genus Halobacillus, with sequence similarity of 98.5-96.0 % to known type strains, showing high sequence similarity to Halobacillus locisalis MSS-155(T) (98.5 %), Halobacillus faecis IGA7-4(T) (98.2 %) and Halobacillus alkaliphilus FP5(T) (98.0 %), and less than 98.0 % sequence similarity to other currently recognised type strains of the genus. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and an unidentified lipid. The cell wall peptidoglycan was found to be based on L-Orn-D-Asp, the predominant isoprenoid quinone was identified as menaquinone-7 (MK-7) and the major fatty acids were identified as anteiso-C15:0, iso-C15:0, anteiso-C17:0 and C16:1 ω7c alcohol. The DNA G+C content of this novel isolate was determined to be 45.3 mol %. Levels of DNA:DNA relatedness between strain TGS-15(T) and the type strains of 13 other species of the genus ranged from 52 to 9 %. On the basis of the polyphasic analysis conducted in this study, strain TGS-15(T) is concluded to represent a novel species of the genus Halobacillus, for which the name Halobacillus salicampi sp. nov. is proposed. The type strain is TGS-15(T) (=KACC 18264(T) = NBRC 110640(T)).
Topics: Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; Geologic Sediments; Halobacillus; Phenotype; Phylogeny; Ponds; Republic of Korea; Salinity; Water Microbiology
PubMed: 26942918
DOI: 10.1007/s10482-016-0672-y -
Antonie Van Leeuwenhoek Nov 2009A moderately halophilic, Gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain JSM 071077(T), was isolated from a subterranean...
A moderately halophilic, Gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain JSM 071077(T), was isolated from a subterranean brine sample collected from a salt mine in Hunan Province, China. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 071077(T) was able to grow with 2-25% (w/v) total salts (optimum, 5-10%), at pH 6.0-10.0 (optimum, pH 7.5) and 10-40 degrees C (optimum, 25-30 degrees C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant menaquinone was MK-7, and the major cellular fatty acids were anteiso-C(15:0), anteiso-C(17:0) and iso-C(15:0). The genomic DNA G+C content was 41.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that strain JSM 071077(T) should be assigned to the genus Halobacillus, being related most closely to the type strain of Halobacillus naozhouensis (98.8% sequence similarity), and the two strains formed a distinct subline in the neighbor-joining, minimum-evolution and maximum-parsimony phylogenetic trees. The sequence similarities between the novel isolate and the type strains of other recognized Halobacillus species ranged from 97.6% (with Halobacillus alkaliphilus) to 95.2% (with Halobacillus kuroshimensis). The results of the phylogenetic analyses, combined with DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic information, support that strain JSM 071077(T) represents a new species of the genus Halobacillus, for which the name Halobacillus hunanensis sp. nov. is proposed. The type strain is JSM 071077(T) (=DSM 21184(T) = KCTC 13235(T)).
Topics: Aerobiosis; Bacillaceae; Base Composition; Catalase; Cell Wall; China; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Diaminopimelic Acid; Fatty Acids; Flagella; Hydrogen-Ion Concentration; Locomotion; Molecular Sequence Data; Oxidoreductases; Phylogeny; RNA, Ribosomal, 16S; Salts; Sequence Analysis, DNA; Soil Microbiology; Spores, Bacterial; Vitamin K 2
PubMed: 19618289
DOI: 10.1007/s10482-009-9365-0