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International Journal of Systematic and... Oct 2009A moderately halophilic, endospore-forming, Gram-positive, catalase- and oxidase-positive, motile, rod-shaped, aerobic bacterium, designated strain JSM 078133(T), was...
A moderately halophilic, endospore-forming, Gram-positive, catalase- and oxidase-positive, motile, rod-shaped, aerobic bacterium, designated strain JSM 078133(T), was isolated from a subterranean brine sample collected from a salt mine in Hunan Province, China. Strain JSM 078133(T) was able to grow with 1-23 % (w/v) total salts (optimum, 5-10 %) and at pH 6.0-10.0 (optimum, pH 7.5) and 10-45 degrees C (optimum, 30 degrees C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant respiratory quinone was menaquinone 7 (MK-7) and the major cellular fatty acids were anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(15 : 0). The genomic DNA G+C content of strain JSM 078133(T) was 42.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 078133(T) belongs to the genus Halobacillus, and was related most closely to the type strains of Halobacillus campisalis (97.1 % sequence similarity), Halobacillus alkaliphilus (97.0 %) and Halobacillus yeomjeoni (96.8 %). The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 078133(T) represents a novel species of the genus Halobacillus, for which the name Halobacillus salsuginis sp. nov. is proposed. The type strain is JSM 078133(T) (=DSM 21185(T)=KCTC 13236(T)).
Topics: Bacillaceae; Base Composition; China; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Geologic Sediments; Mining; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S; Salts; Sodium Chloride
PubMed: 19622644
DOI: 10.1099/ijs.0.010801-0 -
International Journal of Systematic and... Jan 2008A moderately halophilic, spore-forming, Gram-positive, short-rod-shaped bacterium, designated strain MS10(T), was isolated from the surface of leaves of the black...
A moderately halophilic, spore-forming, Gram-positive, short-rod-shaped bacterium, designated strain MS10(T), was isolated from the surface of leaves of the black mangrove Avicennia germinans and was subjected to a polyphasic taxonomic study. Strain MS10(T) was able to grow at NaCl concentrations in the range 5-20% (w/v) with optimum growth at 10% (w/v) NaCl. Growth occurred at temperatures of 10-50 degrees C (optimal growth at 33-35 degrees C) and pH 6.0-9.0 (optimal growth at pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MS10(T) fell within the branch encompassing members of the genus Halobacillus and was most closely related to Halobacillus dabanensis JCM 12772(T) (99.2% 16S rRNA gene sequence similarity). The DNA G+C content of strain MS10(T) was 45.7 mol%, the major respiratory isoprenoid quinone was MK-7 and the cell-wall peptidoglycan was of the L-Orn-D-Asp type, characteristics consistent with its affiliation to the genus Halobacillus. Strain MS10(T) showed a level of DNA-DNA hybridization with H. dabanensis JCM 12772(T) of 29% and levels below 70% were also obtained with respect to other recognized members of the genus Halobacillus. The major fatty acids of strain MS10(T) were iso-C(16:0), anteiso-C(15:0), iso-C(14:0) and iso-C(15:0). Overall, the phenotypic, genotypic and phylogenetic results presented in this study demonstrate that strain MS10(T) represents a novel species of the genus Halobacillus, for which the name Halobacillus mangrovi sp. nov. is proposed. The type strain is MS10(T) (=CECT 7206(T)=CCM 7397(T)).
Topics: Avicennia; Bacillaceae; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Genes, rRNA; Genotype; Molecular Sequence Data; Nucleic Acid Hybridization; Phenotype; Phylogeny; Plant Leaves; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Sodium Chloride; Species Specificity
PubMed: 18175696
DOI: 10.1099/ijs.0.65008-0 -
Microbiology Resource Announcements Feb 2024We report here the genome sequence of moderately halophilic ASL-17, isolated from hypersaline sediment from the Yellow Sea, Korea. The bacterium was Gram variable, oval...
We report here the genome sequence of moderately halophilic ASL-17, isolated from hypersaline sediment from the Yellow Sea, Korea. The bacterium was Gram variable, oval or coccoid, and mesophilic. The genome of ASL-17 has 3.8 Mbp, with 3,910 coding sequences, 76 RNAs, and 41.3% G + C content.
PubMed: 38206017
DOI: 10.1128/mra.00692-23 -
International Journal of Systematic and... Nov 2007A Gram-positive, spore-forming, rod-shaped halophilic bacterial strain, IGA7-4T, was isolated from a mangrove area on Ishigaki Island (Japan), and was characterized...
A Gram-positive, spore-forming, rod-shaped halophilic bacterial strain, IGA7-4T, was isolated from a mangrove area on Ishigaki Island (Japan), and was characterized taxonomically using a polyphasic approach. Strain IGA7-4T was strictly aerobic and non-motile and formed central endospores. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain IGA7-4T is affiliated to the genus Halobacillus, and exhibits sequence similarities of 99.6-98.0% to the type strains of Halobacillus species. Levels of DNA-DNA relatedness between strain IGA7-4T and the type strains of Halobacillus species were 9.5-46.6%. The DNA G+C content of strain IGA7-4T was 46.5 mol%. The cell-wall peptidoglycan type (Orn-Asp), major cellular fatty acids (anteiso-C15:0, anteiso-C17:0, iso-C15:0 and iso-C16:0) and quinone type (MK-7) of the isolate support its affiliation to the genus Halobacillus. On the basis of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data, the isolate represents a novel species of the genus Halobacillus, for which the name Halobacillus faecis sp. nov. is proposed. The type strain is IGA7-4T (=MBIC08268T=IAM 15427T=KCTC 13121T).
Topics: Bacillaceae; Bacterial Typing Techniques; DNA, Ribosomal; Geologic Sediments; Japan; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Rhizophoraceae; Rivers; Sequence Analysis, DNA; Sodium Chloride; Spores, Bacterial
PubMed: 17978203
DOI: 10.1099/ijs.0.64896-0 -
International Journal of Systematic and... Apr 2008A Gram-positive, spore-forming, halophilic bacterial strain, FP5T, was isolated from a salt lake in southern Spain and subjected to a polyphasic taxonomic study. Strain...
A Gram-positive, spore-forming, halophilic bacterial strain, FP5T, was isolated from a salt lake in southern Spain and subjected to a polyphasic taxonomic study. Strain FP5T was strictly aerobic. Cells were coccoidal, occurring singly or in clusters. The cell-wall peptidoglycan type of strain FP5T was A4 beta based on l-Orn-d-Asp. Strain FP5T was characterized chemotaxonomically by having MK-7 as the major menaquinone and anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C16 : 0 as the main fatty acids. The isolate grew optimally at 37 degrees C and in presence of 10 % NaCl; no growth was observed in the absence of NaCl. The DNA G+C content was 43.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FP5T falls within the evolutionary radiation of species of the genus Halobacillus. Levels of 16S rRNA gene sequence similarity between strain FP5T and the type strains of nine recognized Halobacillus species were in the range 97.0-99.0 %. Levels of DNA-DNA relatedness indicated that strain FP5T represents a genomic species that is distinct from recognized Halobacillus species. Strain FP5T could be differentiated from recognized Halobacillus species based on several phenotypic characteristics. On the basis of phenotypic, phylogenetic and genomic data, strain FP5T is considered to represent a novel species of the genus Halobacillus, for which the name Halobacillus alkaliphilus sp. nov. is proposed. The type strain is FP5T (=DSM 18525T =ATCC BAA-1361T).
Topics: Bacillaceae; Base Composition; Base Sequence; DNA Primers; DNA, Bacterial; Fatty Acids; Fresh Water; Genes, Bacterial; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Bacterial; RNA, Ribosomal, 16S; Salinity; Spain; Terminology as Topic
PubMed: 18398188
DOI: 10.1099/ijs.0.65457-0 -
International Journal of Systematic and... Jul 2003A moderately halophilic, gram-positive, spore-forming bacterium was isolated from surface saline soil of the Karaj region, Iran. The strain, designated MA-2T, was...
A moderately halophilic, gram-positive, spore-forming bacterium was isolated from surface saline soil of the Karaj region, Iran. The strain, designated MA-2T, was strictly aerobic with rod-shaped cells that occurred singly, in pairs or short chains. It contained L-Om-D-Asp-type peptidoglycan and the major respiratory lipoquinone was MK-7. It was non-motile and had an ellipsoidal endospore located centrally or subterminally. Growth occurred at 10-49 degrees C and in the pH range 6.0-9.6. Strain MA-2T grew at salinities of 1-24% (w/v) NaCl, showing optimal growth at 10% (w/v). The DNA G + C content was 41.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MA-2T was associated with Bacillus rRNA group 1. The micro-organisms showing the closest phylogenetic relationship to strain MA-2T were Halobacillus litoralis and Halobacillus trueperi. On the basis of phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA-DNA similarity data, it is proposed that strain MA-2T (= DSM 14948T = LMG 21515T) should be placed in the genus Halobacillus as the type strain of a novel species, Halobacillus karajensis sp. nov.
Topics: Bacillaceae; Base Composition; DNA, Bacterial; DNA, Ribosomal; Iran; Microscopy, Electron, Scanning; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Bacterial; RNA, Ribosomal, 16S; Sodium Chloride; Soil Microbiology
PubMed: 12892126
DOI: 10.1099/ijs.0.02448-0 -
International Journal of Systematic and... Mar 2008A Gram-positive or Gram-variable, motile, moderately halophilic, rod-shaped bacterial strain, ISL-50(T), was isolated from a marine solar saltern in Korea and subjected...
A Gram-positive or Gram-variable, motile, moderately halophilic, rod-shaped bacterial strain, ISL-50(T), was isolated from a marine solar saltern in Korea and subjected to a polyphasic taxonomic study. Strain ISL-50(T) grew optimally at pH 7.0-8.0 and 37 degrees C in the presence of 8 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ISL-50(T) belonged to the genus Halobacillus. Strain ISL-50(T) exhibited 16S rRNA gene sequence similarity values of 95.3-98.2 % with respect to the type strains of recognized Halobacillus species. The cell-wall peptidoglycan was based on meso-diaminopimelic acid, the predominant menaquinone was MK-7 and the major fatty acids were anteiso-C(15 : 0) and anteiso-C(17 : 0). The DNA G+C content was 39.3 mol%. DNA-DNA relatedness data and differential phenotypic properties revealed that strain ISL-50(T) was distinguishable from the recognized Halobacillus species. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain ISL-50(T) represents a novel species of the genus Halobacillus, for which the name Halobacillus seohaensis sp. nov. is proposed. The type strain is ISL-50(T) (=KCTC 13145(T)=CCUG 54358(T)).
Topics: Bacillaceae; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Genes, rRNA; Geologic Sediments; Korea; Molecular Sequence Data; Nucleic Acid Hybridization; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Species Specificity
PubMed: 18319467
DOI: 10.1099/ijs.0.65341-0 -
Journal of Applied Microbiology Nov 2022This study examined and characterized the extract for metabolites of Halobacillus marinus HMALI004 to understand their antibacterial activities against opportunistic...
AIMS
This study examined and characterized the extract for metabolites of Halobacillus marinus HMALI004 to understand their antibacterial activities against opportunistic marine pathogens, that is, Vibrio parahaemolyticus and Vibrio cholerae.
METHODS AND RESULTS
The bacterial strain HMALI004 was characterized as H. marinus, and an antibacterial spectral test revealed its inhibition against two opportunistic marine pathogens (V. parahaemolyticus and V. cholera). Fermentation broth of strain HMALI004 was subjected to column chromatography and high-performance liquid chromatography to separate antibacterial substances. Two compounds were successfully isolated and identified as 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid by mass spectrometry (MS) and nuclear magnetic resonance. The minimal inhibition concentration (MIC) values of 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid for V. parahaemolyticus were 25 μg/ml, while their MIC values for V. cholerae were 50 and 100 μg/ml, respectively. The reactive oxygen species (ROS) production of two pathogen strains treated with 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid were detected to investigate the antimicrobial mechanism. The results suggested that 4-chloro-1H-pyrrole-2-carboxylic acid exerted enhanced ROS production in V. parahaemolyticus, whereas 1H-pyrrole-2-carboxylic acid had a weaker effect. Both compounds caused a significant rise in ROS production in V. cholerae, causing severe damage to the cell wall and cytoplasm, leading to cell death.
CONCLUSIONS
The bacterium H. marinus HMALI004 was isolated from a shrimp pond and was found to produce antimicrobial compounds, which could inhibit the growth of opportunistic marine pathogens V. parahaemolyticus and V. cholerae by increasing ROS.
SIGNIFICANCE AND IMPACT OF THE STUDY
Successfully isolated antibacterial-producing strain, H. marinus HMALI004, and its antimicrobial compounds could be used as biological control agents for marine pathogens.
Topics: Halobacillus; Reactive Oxygen Species; Biological Control Agents; Bacteria; Vibrio parahaemolyticus; Vibrio cholerae; Anti-Bacterial Agents; Anti-Infective Agents; Plant Extracts
PubMed: 35929370
DOI: 10.1111/jam.15764 -
International Journal of Systematic and... Jun 2007Two Gram-positive, rod-shaped, moderately halophilic bacteria were isolated from a deep-sea carbonate rock at a methane cold seep in Kuroshima Knoll, Japan. These...
Two Gram-positive, rod-shaped, moderately halophilic bacteria were isolated from a deep-sea carbonate rock at a methane cold seep in Kuroshima Knoll, Japan. These bacteria, strains IS-Hb4(T) and IS-Hb7(T), were spore-forming and non-motile. They were able to grow at temperatures as low as 9 degrees C and hydrostatic pressures up to 30 MPa. Based on high sequence similarity of their 16S rRNA genes to those of type strains of the genus Halobacillus, from 96.4 % (strain IS-Hb7(T) to Halobacillus halophilus NCIMB 9251(T)) to 99.4 % (strain IS-Hb4(T) to Halobacillus dabanensis D-8(T)), the strains were shown to belong to this genus. DNA-DNA relatedness values of 49.5 % and 1.0-33.0 %, respectively, were determined between strains IS-Hb4(T) and IS-Hb7(T) and between these strains and other Halobacillus type strains. Both strains showed the major menaquinone MK7 and L-orn-D-Asp cell-wall peptidoglycan type. Straight-chain C(16 : 0), unsaturated C(16 : 1)omega7c alcohol and C(18 : 1)omega7c and cyclopropane C(19 : 0) cyc fatty acids were predominant in both strains. The DNA G+C contents of IS-Hb4(T) and IS-Hb7(T) were respectively 43.3 and 42.1 mol%. Physiological and biochemical analyses combined with DNA-DNA hybridization results allowed us to place strains IS-Hb4(T) (=JCM 14154(T)=DSM 18394(T)) and IS-Hb7(T) (=JCM 14155(T)=DSM 18393(T)) in the genus Halobacillus as the respective type strains of the novel species Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov.
Topics: Bacillaceae; Bacterial Typing Techniques; Base Composition; Cold Temperature; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Genes, rRNA; Hydrostatic Pressure; Japan; Locomotion; Microscopy, Phase-Contrast; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; Quinones; RNA, Bacterial; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Spores, Bacterial
PubMed: 17551037
DOI: 10.1099/ijs.0.64817-0 -
Peptides Mar 2018Members of the Bacillaceae family, including Bacillus spp., Brevibacillus spp., Paenibacillus spp., Aneurinibacillus sp., and Halobacillus sp., are an important source... (Review)
Review
Members of the Bacillaceae family, including Bacillus spp., Brevibacillus spp., Paenibacillus spp., Aneurinibacillus sp., and Halobacillus sp., are an important source of structurally diverse classes of short peptides of ∼ 30 residues or fewer possessing peculiar and rapid killing activity against various pathogens. Additionally, many have unique structures that enhance resistance to hydrolysis by proteases, and these are ideal therapeutic tools and potential alternatives to current antibiotics. The need for novel antibiotic lead compounds is urgent, and this review summarises 119 Bacillaceae compounds published since 2000, including 12 surfactin-like lipopeptides, 16 iturinic lipopeptides, fengycin C, 33 other cyclic lipopeptides, 26 linear lipopeptides, two thiopeptides, four 2,5-diketopiperazines, 20 typical cyclic peptides, and five standard linear peptides. The current and potential therapeutic applications of these peptides, including structure, antibacterial, antifungal, and antiviral activities, are discussed.
Topics: Animals; Anti-Bacterial Agents; Bacillaceae; Humans; Peptides
PubMed: 29269072
DOI: 10.1016/j.peptides.2017.12.018