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Journal of the American Animal Hospital... 1997Thirty-seven dogs with moderately differentiated, cutaneous mast cell tumors had incomplete surgical excisions as determined by histopathology, but no gross evidence of...
Thirty-seven dogs with moderately differentiated, cutaneous mast cell tumors had incomplete surgical excisions as determined by histopathology, but no gross evidence of tumor. All dogs were irradiated to a total dose of between 46.2 and 48.0 Gy using either an orthovoltage source (n = 20) or a linear accelerator (megavoltage) (n = 17). Radiation was delivered to an area bordered by margins of 3 cm or greater around the surgical scar. The mast cell tumors had not recurred in 97% of dogs by one year after radiation therapy and had not recurred in 93% of dogs by three years after radiation. Both orthovoltage and megavoltage radiation provide excellent local control of moderately differentiated mast cell tumors in dogs.
Topics: Animals; Cell Transformation, Neoplastic; Dermatologic Surgical Procedures; Dog Diseases; Dogs; Dose-Response Relationship, Radiation; Female; Male; Mast-Cell Sarcoma; Radiotherapy, Adjuvant; Skin; Skin Neoplasms
PubMed: 9204467
DOI: 10.5326/15473317-33-4-320 -
American Journal of Veterinary Research Jul 1990In a controlled study, malignant murine P815 mastocytoma cells exposed in vitro to distilled and deionized water died as a result of progressive swelling, degranulation,... (Clinical Trial)
Clinical Trial
In a controlled study, malignant murine P815 mastocytoma cells exposed in vitro to distilled and deionized water died as a result of progressive swelling, degranulation, and membrane rupture. A 90% mean cell death occurred when cells obtained directly from culture were exposed to deionized water for 2 minutes. Of 6 cryopreserved malignant murine cell lines, which included Cloudman S91 melanoma, CMT-93 rectum carcinoma, MMT-06052 mammary carcinoma, and S-180 Sarcoma, only P815 mastocytoma and YAC-1 lymphoma were significantly (P less than 0.05) affected by hypotonic shock; Cloudman S91 melanoma cells were the most resistant. Mastocytoma cells were selectively killed by hypotonic solution, and lymphoma cells were also killed by isotonic saline solution. Local mast cell tumor (MCT) recurrence and percentage survival were evaluated in 12 cats (21 MCT) and 54 dogs (85 MCT) subjected to surgery alone or local infiltration of deionized water as an adjunct to surgery. Of all 16 incompletely excised MCT in cats, there was no local recurrence following injection. Four mast cell tumors (2 cats) regressed after being injected in situ. In dogs with clinical stage-I MCT, local recurrence was detected in 50% (5/10), but with injection after incomplete excision, local MCT recurrence was significantly (P less than 0.05) less (6.6%, 1/15). Percentage recurrence was significantly (P less than 0.05) less and survival significantly greater when incompletely excised grade-II MCT were injected. Mean follow-up period after surgery in cats and dogs was 35 and 23.4 months, respectively.
Topics: Animals; Cat Diseases; Cats; Clinical Trials as Topic; Dog Diseases; Dogs; Hypotonic Solutions; Mast-Cell Sarcoma; Neoplasm Recurrence, Local; Prospective Studies; Skin Neoplasms; Time Factors; Tumor Cells, Cultured; Water
PubMed: 2117868
DOI: No ID Found -
The Journal of Small Animal Practice Jun 2009To evaluate the response of measurable canine mast cell tumours unsuitable for other treatment modalities to a chemotherapy protocol comprising chlorambucil and...
OBJECTIVES
To evaluate the response of measurable canine mast cell tumours unsuitable for other treatment modalities to a chemotherapy protocol comprising chlorambucil and prednisolone.
METHODS
Dogs bearing measurable mast cell tumours, unsuitable for treatment by surgery or radiotherapy, were treated with orally administered prednisolone and chlorambucil, and their responses assessed.
RESULTS
Twenty-one dogs were enrolled in the study; 13 had intermediate-grade mast cell tumour, six were high grade and two were diagnosed by cytology alone. Eight dogs had multiple tumours and 13 dogs had single tumours, and six dogs had lymph node metastases and no dogs had visceral metastases detected. Three dogs achieved complete remission, five achieved partial remission (overall response rate 38 per cent), nine had static disease and four dogs had progressive disease. Median progression-free interval for the eight responders was 533 days, and median survival time for all dogs in the study was 140 days. Progression-free interval and median survival time were not influenced by the age, sex, weight or neutering status of the patient, by the grade or stage of the tumour or whether the patient had single or multiple tumours. No toxicity was detected.
CLINICAL SIGNIFICANCE
Response and survival rates of inoperable canine MCT to chlorambucil and prednisolone are comparable to previously described protocols, with no apparent toxicity.
Topics: Animals; Antineoplastic Agents, Alkylating; Antineoplastic Agents, Hormonal; Antineoplastic Combined Chemotherapy Protocols; Chlorambucil; Disease Progression; Dog Diseases; Dogs; Female; Lymphatic Metastasis; Male; Mast-Cell Sarcoma; Neoplasm Staging; Prednisolone; Prospective Studies; Skin Neoplasms; Survival Analysis; Treatment Outcome
PubMed: 19527421
DOI: 10.1111/j.1748-5827.2009.00732.x -
Journal of the American Veterinary... Jun 1987Cutaneous mast cell neoplasia was diagnosed in 14 cats from January 1975 to September 1985. Review of the available medical records and client communication provided...
Cutaneous mast cell neoplasia was diagnosed in 14 cats from January 1975 to September 1985. Review of the available medical records and client communication provided information on tumor description, location, and recurrence, and each cat's current status. Twenty-three cutaneous mast cell neoplasms were surgically excised, examined, and graded histologically. In this study, feline cutaneous mast cell tumors did not metastasize to lymph nodes or viscera, did not recur at a previous excision site, and did not cause or contribute to the death of any cat. A histologic grading system described for canine mast cell tumors apparently provided no prognostic information for the cats in this study. There was a predilection for older male cats to develop cutaneous mast cell tumors, especially on the head and neck.
Topics: Animals; Cat Diseases; Cats; Female; Follow-Up Studies; Male; Mast-Cell Sarcoma; Recurrence; Retrospective Studies; Skin Neoplasms
PubMed: 3112049
DOI: No ID Found -
Journal of the American Veterinary... Oct 1988Ninety-five mast cell tumors in 85 dogs were therapeutically irradiated. Median and mean tumor-free times for dogs were 17 and 62.7 months, respectively. Percentages of...
Ninety-five mast cell tumors in 85 dogs were therapeutically irradiated. Median and mean tumor-free times for dogs were 17 and 62.7 months, respectively. Percentages of dogs tumor-free at 1 and 2 years were 78.8 and 77%, respectively. Factors significantly affecting tumor-free time were clinical stage (P less than 0.001) and neoplasm location (P = 0.019). Median and mean survival times were 19 and 61.2 months, respectively. Survival rates at 1 and 2 years were 76.2 and 73.2%, respectively. Prognostic factors that significantly affected survival rates were clinical stage (P less than 0.001), neoplasm grade (P = 0.006), and neoplasm location (P = 0.034). Radiation therapy was an effective treatment of mast cell tumor in dogs.
Topics: Animals; Dog Diseases; Dogs; Mast-Cell Sarcoma; Prognosis; Skin Neoplasms
PubMed: 3142828
DOI: No ID Found -
Journal of the American Veterinary... Dec 1999To determine whether immunohistochemical detection of proliferating cell nuclear antigen (PCNA) and Ki-67 correlated with prognosis for dogs with cutaneous mast cell...
OBJECTIVE
To determine whether immunohistochemical detection of proliferating cell nuclear antigen (PCNA) and Ki-67 correlated with prognosis for dogs with cutaneous mast cell tumors (MCT).
DESIGN
Case series.
ANIMALS
120 dogs with solitary cutaneous MCT that were excised.
PROCEDURE
Information on signalment, history, and outcome was obtained by sending a questionnaire to referring veterinarians. Tumors were graded histologically, and immunohistochemical staining for Ki-67 and PCNA was performed.
RESULTS
Survival rates 12, 18, and 24 months after surgery were significantly different among groups when dogs were grouped on the basis of histologic grade. Although mean number of PCNA-positive nuclei/1,000 tumor nuclei was significantly higher for dogs that died of MCT than for those that survived, there was great overlap in values. Mean number of Ki-67-positive nuclei/1,000 tumor nuclei was significantly higher for dogs that died of MCT than for those that survived, without any overlap in values between groups, and number of Ki-67-positive nuclei/1,000 tumor nuclei was significantly different among groups when tumors were grouped on the basis of histologic grades. For dogs with grade-II tumors, number of Ki-67-positive nuclei/1,000 tumor nuclei (< 93 vs > or = 93) was significantly associated with outcome (survived vs died).
CONCLUSIONS AND CLINICAL RELEVANCE
Results suggest that for dogs with solitary cutaneous MCT, determining number of Ki-67-positive nuclei may be useful in predicting prognosis, particularly for dogs with grade-II tumors.
Topics: Animals; Biomarkers, Tumor; Dog Diseases; Dogs; Female; Follow-Up Studies; Immunohistochemistry; Ki-67 Antigen; Male; Mast-Cell Sarcoma; Neoplasm Staging; Prognosis; Proliferating Cell Nuclear Antigen; Skin Neoplasms; Survival Analysis; Time Factors
PubMed: 14567425
DOI: No ID Found -
Veterinary Pathology Nov 1993The study of the signalment, histomorphologic features, and post-surgical clinical progress of 57 cutaneous neoplasms in 55 domestic ferrets (Mustela putorius furo L.)... (Review)
Review
The study of the signalment, histomorphologic features, and post-surgical clinical progress of 57 cutaneous neoplasms in 55 domestic ferrets (Mustela putorius furo L.) was based on diagnostic pathologic accessions (1987-1992) from 142 ferrets. Mean age of the group was 4.3 years; 31/54 (57%) were female and 23/54 (43%) were male. Thirty-three (58%) of the cutaneous neoplasms were basal cell tumors. The mean age of ferrets with basal cell tumor was 5.2 years, and 23/33 (70%) were female. Histologically, the basal cell tumors were composed of well-differentiated basaloid epithelial cells with various degrees of squamous and sebaceous differentiation, similar to those seen in basal cell neoplasms of dogs. Nine of the 57 (16%) cutaneous neoplasms were mastocytomas. The mean age of ferrets with mastocytoma was 4.1 years; four were male, four were female, and the sex of one was unrecorded. Histologically, the mastocytomas were composed of well-differentiated mast cells with few eosinophils, similar to cutaneous mastocytomas of domestic cats. The mast cells had a small number of metachromatic cytoplasmic granules, and in six of eight neoplasms the granules had an affinity for conjugated avidin-peroxidase. Six of the cutaneous neoplasms (11%) were fibromas. The mean age of ferrets with fibroma was 2.7 years; 5 (83%) were male. Two cutaneous hemangiomas (4%) were in females, which were 4 and 5 years of age. There was one each hemangiosarcoma, cutaneous polyp, anal gland adenocarcinoma, lymphosarcoma of the prepuce and inguinal lymph node, and adenocarcinoma of the prepuce.
Topics: Animals; Female; Ferrets; Fibroma; Male; Mast-Cell Sarcoma; Neoplasms, Basal Cell; Skin Neoplasms; Treatment Outcome
PubMed: 8116142
DOI: 10.1177/030098589303000602 -
American Journal of Medical Genetics Aug 1991Peripheral blood lymphocytes from boxer dogs with a history of cutaneous mast cell tumors were cultured for fragile site expression. As in a control group of dogs, cells...
Peripheral blood lymphocytes from boxer dogs with a history of cutaneous mast cell tumors were cultured for fragile site expression. As in a control group of dogs, cells from these dogs expressed folate-sensitive autosomal and X chromosome fragile sites. Cells from boxer dogs with mast cell tumors expressed the same three common fragile sites on the X chromosome as cells from control dogs. Three folate-sensitive autosomal fragile sites not observed in cells from the control dogs were identified in cells from boxers with mast cell tumors. These included fragile sites near the telomeres of the arms of chromosomes 3 and 4 and a fragile site on the distal half of chromosome 15. Cells from boxers with mast cell tumors showed a greater frequency of fragile site expression than did cells from control dogs, but this observation was attributed to an unintended selection bias for younger boxer dogs without mast cell tumors and older boxer dogs with mast cell tumors and an increased frequency of fragile site expression with increasing age in dogs of the boxer breed.
Topics: Aging; Animals; Chromosome Fragile Sites; Chromosome Fragility; Cytogenetics; Disease Susceptibility; Dog Diseases; Dogs; Female; Folic Acid; Genetic Variation; Lymphocytes; Male; Mast-Cell Sarcoma; Risk; Tumor Cells, Cultured; X Chromosome
PubMed: 1910264
DOI: 10.1002/ajmg.1320400220 -
Problemy Gematologii I Perelivaniia... Dec 1978
Topics: Adult; Diagnosis, Differential; Female; Humans; Leukemia, Myeloid; Mast Cells; Mast-Cell Sarcoma; Skin Neoplasms; Urticaria Pigmentosa
PubMed: 106377
DOI: No ID Found -
Human Pathology Jun 1973
Review
Topics: Child; Diagnosis, Differential; Humans; Hyperplasia; Leukemia; Lymphatic Diseases; Mast Cells; Mast-Cell Sarcoma; Skin; Skin Diseases; Skin Neoplasms; Syndrome; Telangiectasis; Urticaria Pigmentosa
PubMed: 4196238
DOI: 10.1016/s0046-8177(73)80010-3