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Food Chemistry Jan 2022Carbendazim (CBZ) is a fungicide employed in grape crop disease controls, and its maximum residue limit in food is regulated by specialized agencies. This study aimed to...
Carbendazim (CBZ) is a fungicide employed in grape crop disease controls, and its maximum residue limit in food is regulated by specialized agencies. This study aimed to determine the CBZ content in the grape juices in a semi-quantitative classification model based on portable Ultraviolet-Visible spectroscopy and partial least squares with discriminant analysis. The sensitivity and specificity of the obtained model ranged from 83 to 100%, with the external validation set. These results are therefore promising for industrial application, and the model presents robustness for the evaluation of grape juices produced from a different grape variety. The VIP scores allowed identifying important variables involved in class modeling. This study suggests a methodology that is fast and demands minimal sample preparation (only dilution), besides being less expensive compared to the traditional methods, free of reagent/solvent, contributing to quality control in the juice industry.
Topics: Benzimidazoles; Carbamates; Fruit and Vegetable Juices; Least-Squares Analysis; Vitis
PubMed: 34416485
DOI: 10.1016/j.foodchem.2021.130742 -
Environmental Pollution (Barking, Essex... Jan 2023A sensitive and stable substrate plays a vital role in the Raman spectroscopic techniques as an analytical method for detecting pesticides effectively from the...
A sensitive and stable substrate plays a vital role in the Raman spectroscopic techniques as an analytical method for detecting pesticides effectively from the environment. Enhancing signals from nanoparticles are weak and inconsistent in repeatability since analytes tend to degrade quickly under laser exposure. Herein, a novel substrate of Au@ZIF-67 is prepared on octahedral AuNPs by trapping pesticide molecules with small three-dimensional volumes by the flexibility of ZIF-67 for rapid detection with high sensitivity and stability. The two types of thiram and carbendazim pesticides, which are environmental pollutants that affect biodiversity, were successfully absorbed in Au@ZIF-67 nanostructures by adsorption-desorption equilibrium for analytical purposes in Raman spectroscopy. Spectra calculations of the thiram and carbendazim molecules on 8 atoms of Au using DFT were compared with the experimental data. The SERS enhancement factors for thiram and carbendazim were estimated to be 1.91 × 10 and 3.12 × 10, respectively, with the LOD values of trace amounts of ∼10 mol L. The novel substrate of Au@ZIF-67 is a propitious platform for detecting thiram and carbendazim in trace amounts, providing a helpful strategy for detecting residues with high performance in the environment at the laboratory and practical scales.
Topics: Pesticides; Thiram; Environmental Pollutants; Gold; Metal Nanoparticles; Spectrum Analysis, Raman
PubMed: 36455771
DOI: 10.1016/j.envpol.2022.120775 -
Environmental Toxicology and Chemistry Feb 2017Carbendazim is a fungicide largely used in agriculture as a plant protection product. As a result of agricultural runoffs, drainage, and leaching, it reaches surface...
Carbendazim is a fungicide largely used in agriculture as a plant protection product. As a result of agricultural runoffs, drainage, and leaching, it reaches surface waters at concentrations possibly hazardous to aquatic communities. Because of potential and continuous release of carbendazim to aquatic systems, long-term exposure to aquatic organisms should be addressed. To fill the knowledge gap, the present study evaluated the responses of multiple generations of Daphnia magna (clone K6) to an environmentally relevant concentration of carbendazim (5 μg/L). Twelve successive generations were evaluated, and the effects in these offspring were compared with those from a control population. Neonates' fitness was assessed through immobilization, reproduction, and feeding activity tests, along with the comet assay for in vivo DNA damage evaluation. Recovery from long-term exposure was also assessed. In the F5 generation, the results revealed that when daphnids were re-exposed to carbendazim, DNA damage was higher in daphnids continuously exposed to carbendazim than those from clean medium. After daphnids were moved to a clean medium, a low recovery potential was observed for DNA damage. Daphnids exposed continuously for 6 generations (F6) to carbendazim displayed an increase in feeding rates when re-exposed to carbendazim compared with F6 daphnids reared in clean medium. Continuous exposure of daphnids to carbendazim induced a significant increase in DNA damage from the F0 to the F12 generation. Deleterious effects of the multigenerational exposure to carbendazim were more prominent at a subcellular level (DNA damage) compared with the individual level. Environ Toxicol Chem 2017;36:383-394. © 2016 SETAC.
Topics: Animals; Benzimidazoles; Carbamates; DNA Damage; Daphnia; Fungicides, Industrial; Reproduction; Water Pollutants, Chemical
PubMed: 27349362
DOI: 10.1002/etc.3541 -
Journal of Food and Drug Analysis Jun 2023Green emission carbon dots (CDs) electrochemically prepared from 2,6-pyridinedicarboxylic acid and o-phenyl-enediamine were applied separately for the quantitation of...
Green emission carbon dots (CDs) electrochemically prepared from 2,6-pyridinedicarboxylic acid and o-phenyl-enediamine were applied separately for the quantitation of hypochlorite and carbendazim. The characteristic and optical properties of the CDs were studied through fluorescence, UV-vis absorption, X-ray photoelectron spectroscopy, and transmission electron microscopy. The synthesized CDs were mainly 0.8-2.2 nm in size, with an average size of 1.5 nm. The CDs exhibited green luminescence centered at 520 nm when excited by 420 nm light. The green emission of the CDs is quenched after the addition of hypochlorite, mainly through the redox reaction between hypochlorite and hydroxyl groups on the CDs surface. Furthermore, the hypochlorite-induced fluorescence quenched can be prevented in the presence of carbendazim. The sensing approaches exhibit good linear ranges of 1-50 μM and 0.05-5 μM for hypochlorite and carbendazim, respectively, with low detection limits of 0.096 and 0.005 μM, respectively. Practicalities of the luminescent probes were separately validated by the quantitation of the two analytes in real sample matrix with recoveries ranging from 96.3 to 108.9% and the relative standard deviation values below 5.51%. Our results show the potential of the sensitive, selective, and simple CD probe for water and food quality control.
Topics: Hypochlorous Acid; Quantum Dots; Carbon; Photoelectron Spectroscopy
PubMed: 37335167
DOI: 10.38212/2224-6614.3445 -
Food Chemistry Jul 2022In this study, a platinum-doped nickel cobaltite nanograss (Pt-doped NiCoO NG) with its own unique structural features was initially synthesized, utilizing a simple...
In this study, a platinum-doped nickel cobaltite nanograss (Pt-doped NiCoO NG) with its own unique structural features was initially synthesized, utilizing a simple hydrothermal method and then applied as a novel platform for the detection of carbendazim (CHNO; CBZ). To this end, the CBZ electrochemical signals were evaluated by means of differential pulse voltammetry (DPV), demonstrating the acceptable catalytic effect of the Pt-doped NiCoO NG/screen-printed electrode (SPE) on the CBZ oxidation signal. Under the optimized conditions, CBZ was subsequently quantified by the Pt-doped NiCoO NG/SPE with a wide linear range (0.03-140 μM) and a low limit of detection (LOD) value (0.005 μM). The proposed sensor was thus characterized by good anti-interference ability, selectivity, and stability. The analysis of the real samples, viz. tomato and lettuce, also confirmed that the given sensor had good recoveries and relative standard deviation (RSD). Ultimately, a comparison between liquid chromatography-mass spectrometry (LC-MS) and this method established no significant difference in the results.
Topics: Benzimidazoles; Carbamates; Electrochemical Techniques; Electrodes; Platinum
PubMed: 35183970
DOI: 10.1016/j.foodchem.2022.132398 -
Xenobiotica; the Fate of Foreign... Sep 1986The disappearance of 14C-carbendazim in rat (i.v. 12 mg/kg) followed the kinetics of a two-compartment open-system model. Half-lives of the alpha-phase were 0.1 h...
The disappearance of 14C-carbendazim in rat (i.v. 12 mg/kg) followed the kinetics of a two-compartment open-system model. Half-lives of the alpha-phase were 0.1 h (blood), 0.16 h (liver), 0.25 h (kidney), and of the beta-phase: 2.15 h, 6.15 h, respectively. Two metabolites: methyl 5-hydroxy-2-benzimidazolecarbamate (5-HBC) and 2-aminobenzimidazole (2-AB) were formed very rapidly. Their peak concentrations in liver and kidney were 15 min after i.v. injection. Unchanged carbendazim was found in highest concentrations in blood. 5-HBC prevails in organs. 2-AB was present only in minor amounts. The extent of bioavailability in orally administered 14C-carbendazim (12 mg/kg) was about 85%. The disposition of radioactivity in subcellular fractions was not uniform, its highest concentration was in cytosol, the lowest in microsomes. The elimination of 14C-carbendazim in urine is biphasic. Half-lives of the alpha-phase were 1.4 h (i.v.) and 2.5 h (oral), and of the beta-phase 11.2 h and 12.1 h, respectively. Irrespective of the route of administration, 95% of the radioactivity in urine was composed of 5-HBC. The concentration of unchanged carbendazim in blood and of 5-HBC in urine may be of diagnostic value in acute poisoning with carbendazim.
Topics: Administration, Oral; Animals; Benzimidazoles; Biotransformation; Body Fluids; Carbamates; Injections, Intravenous; Intestinal Absorption; Male; Microsomes, Liver; Rats; Time Factors; Tissue Distribution
PubMed: 3765661
DOI: 10.3109/00498258609038962 -
Journal of Hazardous Materials Sep 2023The plant microbiota can substantially contribute to various functions related to host health, fitness, and productivity. Therefore, maintaining the integrity of the...
The plant microbiota can substantially contribute to various functions related to host health, fitness, and productivity. Therefore, maintaining the integrity of the microbiota is beginning to be seen as a crucial factor in modern agriculture. Here, we evaluated the effects of two chemical pesticides (azoxystrobin and carbendazim) and an antibiotic-based biopesticide (wuyiencin) on the rhizosphere microbiome of tomato plants. It was found that all treatments resulted in changes in the bacterial community structure to varying degrees. The most pronounced changes were observed with the biopesticide, which resulted in an enrichment of Streptomyces in the microbiome. In contrast, the relative abundance of Actinobacteria decreased in samples that were treated with low and high dosages of carbendazim. Clostridia were enriched after the applications of azoxystrobin and wuyiencin. When functioning of the microbiome was assessed, it was shown that genes encoding multidrug efflux pumps and ABC transporters related to nutrient uptake were enriched. This enrichment is likely to overcome potentially negative effects linked to the exposure to the employed substances. The study provides new insights into the potential of different pesticides to modulate native plant microbiomes, and thus highlights the importance to include such evaluations when new active agents are developed.
Topics: Biological Control Agents; Soil Microbiology; Pesticides; Rhizosphere; Anti-Bacterial Agents; Bacteria; Plant Roots
PubMed: 37453358
DOI: 10.1016/j.jhazmat.2023.132035 -
Scientific Reports Sep 2022Different pollutants usually co-exist in the natural environment, and the ecological and health risk assessment of agrochemicals needs to be carried out based on the...
Different pollutants usually co-exist in the natural environment, and the ecological and health risk assessment of agrochemicals needs to be carried out based on the combined toxicological effects of pollutants. To examine the combined toxicity to aquatic organisms, the effects of cadmium (Cd) and five pesticides (acetamiprid, carbendazim, azoxystrobin, chlorpyrifos, and bifenthrin) mixture on zebrafish (Danio rerio) larvae were assessed. The data from the 96-h toxicity test indicated that bifenthrin possessed the highest toxicity to D. rerio with the LC value of 0.15 mg L, followed by chlorpyrifos (0.36 mg L) and azoxystrobin (0.63 mg L). Cd (6.84 mg L) and carbendazim (8.53 mg L) induced the intermediate toxic responses, while acetamiprid (58.39 mg L) presented the lowest toxicity to the organisms. Pesticide mixtures containing chlorpyrifos and bifenthrin or acetamiprid and carbendazim showed synergistic impacts on the zebrafish. Besides, two binary combinations of Cd-acetamiprid and Cd-chlorpyrifos also displayed a synergistic effect on D. rerio. Our results offered a better idea of the mixed ecological risk assessment of Cd and different agricultural chemicals to aquatic organisms. Our findings better interpreted how the interaction between Cd and various agrochemicals changed their toxicity to aquatic vertebrates and provided valuable insights into critical impacts on the ecological hazard of their combinations.
Topics: Agrochemicals; Animals; Benzimidazoles; Cadmium; Carbamates; Chlorpyrifos; Environmental Pollutants; Larva; Pesticides; Pyrethrins; Pyrimidines; Strobilurins; Water Pollutants, Chemical; Zebrafish
PubMed: 36163367
DOI: 10.1038/s41598-022-20364-8 -
Environment International Jan 2023As an indispensable type of pesticide, fungicides have been somewhat neglected compared to insecticides and herbicides. Heavy fungicide application in agricultural...
As an indispensable type of pesticide, fungicides have been somewhat neglected compared to insecticides and herbicides. Heavy fungicide application in agricultural regions may generate downstream ecological concerns via in-stream transport, and the reservoir complicates the process. Monitoring fungicide exposure and exploring reservoir effect on fungicide transport is the key to develop the downstream strategies of agricultural diffusion pollution control. Here, we investigated the exposure, spatiotemporal dynamics, and ecological risk of fungicides in a reservoir-regulated agricultural basin, located in the middle of the Yangtze River Basin, China. Seven fungicides were preliminarily identified and exhibited high detection frequencies (>85 %) in subsequent quantification of water samples from three sampling activities. The total concentration of fungicides ranged from 2.47 to 560.29 ng/L, 28.35 to 274.69 ng/L, and 13.61 to 146.968 ng/L in April, September, and November, respectively. Overall, the contamination levels of fungicides were in the ascending order of April < November < September. The spatial distribution of fungicides was closely associated with the dense of cultivated land, supporting its agricultural source. Furthermore, the reservoir plays a retention role in fungicides, alleviating ecological pressure downstream during the water storage period. Yet, due to the alternation of "source" and "sink" function of the reservoir, the contribution of Zijiang River to the fungicide load in the Yangtze River Basin still needs further attention. Although there is no acute risk posed by fungicides, even in the high-exposure scenario, the chronic ecological risk could not be ignored. Agricultural intensive regions, coupled with the reservoir, provide rather substantial chronic ecological concerns. Carbendazim has been designated as a priority pollutant that contributes significantly to cumulative chronic risk. Thus, we emphasize strengthening the supervision of fungicides in surface water and rationally restricting the use of carbendazim in agricultural operations.
Topics: Fungicides, Industrial; Water Pollutants, Chemical; Environmental Monitoring; Water; China; Risk Assessment
PubMed: 36535191
DOI: 10.1016/j.envint.2022.107697 -
Mikrochimica Acta Mar 2024The increasing incidence of environmental concerns related to excessive use of pesticides, such as imidacloprid and carbendazim, poses risks to pollinators, water...
The increasing incidence of environmental concerns related to excessive use of pesticides, such as imidacloprid and carbendazim, poses risks to pollinators, water bodies, and human health, prompting regulatory scrutiny and bans in developed countries. In this study, we propose a portable smartphone-based biosensor for rapid and label-free colorimetric detection by using the gold-decorated polystyrene microparticles (Ps-AuNP) functionalized with specific aptamers to imidacloprid and carbendazim on a microfluidic paper-based analytical device (μ-PAD). Four aptamers were selected for the detection of these pesticides and their sensitivity and selectivity performance was evaluated. The sensitivity results show a detection limit for imidacloprid of 3.12 ppm and 1.56 ppm for carbendazim. The aptamers also exhibited high selectivity performance against other pesticides, such as thiamethoxam, fenamiphos, isoproturon, and atrazine. However, the platform presented cross-selectivity when detecting imidacloprid, carbendazim, and linuron, which is discussed herein. Overall, we present a promising platform for simple, on-site, and rapid colorimetric screening of specific pesticides, while highlighting the challenges of aptasensors in achieving selectivity amidst diverse molecular structures.
Topics: Benzimidazoles; Carbamates; Gold; Neonicotinoids; Nitro Compounds; Pesticides; Smartphone
PubMed: 38472537
DOI: 10.1007/s00604-024-06255-x