-
Analytical Chemistry Jan 2023Pressure has been a facile signal readout mode for developing point-of-care testing devices due to the attractive features of portability, accessibility, rapidity, and...
Pressure has been a facile signal readout mode for developing point-of-care testing devices due to the attractive features of portability, accessibility, rapidity, and affordability. Herein, a pressure signal readout device was designed by integrating two homemade needle-type piezoresistive transducers, a controller for a thin-film piezoresistive sensor and a smartphone. Meanwhile, a bidirectional immunochromatographic test strip was designed as an immunoreaction platform for dual-analyte detection. Using PdCuPt nanoparticles with catalase-mimic activity as signal tags, the pressure signals triggered by catalyzed aerogenous reaction were monitored by the pressure signal readout device and read on a smartphone with the Bluetooth module. In this proof-of-principle work, imidacloprid and carbendazim were detected as model analytes. The dynamic ranges for quantitating imidacloprid and carbendazim are 20 pg mL to 50 ng mL and 50 pg mL to 50 ng mL, respectively. The whole immunoassay process was completed within 16 min. The recovery values for imidacloprid and carbendazim spiked into herbal medicines are 82.0-110.0 and 84.0-116.0%, respectively, verifying its reliability for real sample detection. As the smartphone APP and controller for a thin-film piezoresistive sensor contain 12 signal channels, the system can be easily extended to meet the demand for high-throughput screening.
Topics: Smartphone; Reproducibility of Results; Benzimidazoles; Immunoassay; Limit of Detection
PubMed: 36575992
DOI: 10.1021/acs.analchem.2c04322 -
Food Chemistry Jul 2021The detection of carbendazim (CBZ) is important for food safety and human health. However, most current analytical methods require large instruments and highly trained...
The detection of carbendazim (CBZ) is important for food safety and human health. However, most current analytical methods require large instruments and highly trained operators. In order to solve this problem, herein, an innovative portable and quantitative photothermal assay platform relying on a thermometer readout for the detection of CBZ has been developed. Gold nanoparticles (AuNPs), which exhibit a strong distance-dependent photothermal effect under specific laser irradiation, were utilized as indicators. The CBZ aptamer was introduced to protect AuNPs from salt-mediated aggregation. When CBZ is present, the binding event between CBZ and aptamer leads to the loss of the aptamer protective effect on AuNPs, and AuNP aggregation occurs. Under 650-nm laser irradiation, the increase in temperature associated with an AuNP-dependent photothermal effect is highly related to the CBZ concentration. Having the advantages of user-friendliness, low cost, quick response, and portability, this method has great potential for on-site applications.
Topics: Aptamers, Nucleotide; Benzimidazoles; Brassica; Carbamates; Citrus; Food Analysis; Fruit; Fungicides, Industrial; Gold; Metal Nanoparticles; Spectrophotometry, Ultraviolet; Temperature; Thermometers
PubMed: 33626465
DOI: 10.1016/j.foodchem.2021.129292 -
Chemosphere Aug 2022Carbendazim (CAR) as a fungicide is utilized for fruits and vegetables to provide diseases' control and the degradation of carbendazim having benzimidazole ring is slow....
Carbendazim (CAR) as a fungicide is utilized for fruits and vegetables to provide diseases' control and the degradation of carbendazim having benzimidazole ring is slow. Herein, a molecularly imprinted electrochemical sensor based on CdMoO/g-CN nanocomposite was prepared for CAR determination in fruit juice samples. Firstly, CdMoO/g-CN nanocomposite with high yield was fabricated via one-pot in-situ hydrothermal approach including environmentally friendly method. Formation of CAR imprinted polymers was performed on CdMoO/g-CN nanocomposite modified glassy carbon electrode (GCE) in presence of CAR as template and pyrrole (Py) as a monomer by cyclic voltammetry (CV) technique. Following the morphological, structural, and optical characterization of as-synthesized nanocomposite, the electrochemical techniques were also implemented to evaluate the electrochemical features of fabricated electrodes. The limit of quantification (LOQ) and limit of detection (LOD) values were calculated as 0.1 × 10 M, and 2.5 × 10 M, respectively in addition to satisfactory selectivity, stability, reproducibility and reusability. The findings revealed that the proposed CAR imprinted electrochemical sensor can be successfully employed in food safety.
Topics: Benzimidazoles; Carbamates; Electrochemical Techniques; Electrodes; Fruit and Vegetable Juices; Limit of Detection; Molecular Imprinting; Nanocomposites; Reproducibility of Results
PubMed: 35490760
DOI: 10.1016/j.chemosphere.2022.134766 -
Food Chemistry Sep 2022The monitoring of multiple pesticides commonly used in food is a prerequisite for public health safety. Herein, a multiplexed biosensor based on fluorescence resonance...
The monitoring of multiple pesticides commonly used in food is a prerequisite for public health safety. Herein, a multiplexed biosensor based on fluorescence resonance energy transfer (FRET) from multicolor upconversion nanoparticles (UCNPs)to single black phosphorus nanosheets (BPNSs) was successfully developed for simultaneous and selective detection of paraquat and carbendazim pesticides. Due to the strong π-π stacking interactions, aptamers functionalized UCNPs may adsorb on the BPNSs surface, allowing strong upconversion fluorescence quenching. In the presence of paraquat and carbendazim, the aptamers preferentially integrated with their corresponding targets and altered the aptamer's conformation, restoring the fluorescence. An excellent linear correlation was observed from 1.0 to 1.0 × 10 ng/mL, with a limit of detection of 0.18 ng/mL for paraquat and 0.45 ng/mL for carbendazim. The developed aptasensor was further validated by commercial enzyme-linked immunoassays without significant differences in practical detection. Additionally, this work offers new insights into monitoring multiple targets simultaneously.
Topics: Aptamers, Nucleotide; Benzimidazoles; Biosensing Techniques; Carbamates; Fluorescence Resonance Energy Transfer; Limit of Detection; Paraquat; Pesticides
PubMed: 35483279
DOI: 10.1016/j.foodchem.2022.132950 -
Food Chemistry Oct 2023Indicator replacement assay combining with fluorescence resonance energy transfer (FRET) effect has attractive performance in sensing small molecules, however, there...
Indicator replacement assay combining with fluorescence resonance energy transfer (FRET) effect has attractive performance in sensing small molecules, however, there wasn't application in pesticide molecule sensing reported so far. In this work, we prepared a nanocomplex (NCP), AuAgNCs-CD, through self-assembly of gold nanoclusters (AuNCs), silver nanoclusters (AgNCs) and carboxymethyl-β-cyclodextrin (CM-β-CD) by one-step method. The emission of AuNCs was significantly enhance. It was found that FRET between the AuAgNCs-CD and rhodamine B (RhB) existed after AuAgNCs-CD combined with RhB. And carbendazim (CBZ) could induce anti-FRET effect through competing with RhB and binding to AuAgNCs-CD. Thus, this phenomenon was utilized to develop a ratiometric fluorescent detection of CBA. This method was applied in food sample detection and reliable results were obtained. Due to high sensitivity, rapidness and good selectivity, this ratiometric fluorescent method was expected to hold high application potential in monitoring CBZ in foods.
Topics: Metal Nanoparticles; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Benzimidazoles; Gold; Spectrometry, Fluorescence
PubMed: 37267653
DOI: 10.1016/j.foodchem.2023.136478 -
Plant Disease Oct 2019Fusarium head blight, also called scab, is caused by and is one of the most important destructive diseases of wheat. The frequency of carbendazim resistance in 1,132...
Fusarium head blight, also called scab, is caused by and is one of the most important destructive diseases of wheat. The frequency of carbendazim resistance in 1,132 isolates of recovered from fields in different regions of Henan Province in 2016, 2017, and 2018 was determined. A total of 31 isolates resistant to carbendazim were detected, including 30 moderately resistant isolates and one highly resistant isolate. The frequency of resistance of isolates to carbendazim was 2.7%. The range of effective concentration (EC) values of 1,101 sensitive isolates and 30 moderately resistant isolates was 0.08 to 0.98 μg ml and 2.73 to 13.28 μg ml, respectively. The mean ± SD EC value was 0.55 ± 0.13 μg ml and 5.61 ± 2.58 μg ml, respectively. The EC value of the highly resistant isolate was 21.12 μg ml. Point mutation types of the carbendazim-resistant isolates were characterized by cloning the β-tubulin gene of 31 resistant isolates. Three point mutation types at amino acids F167Y, E198Q, and E198L in the β-tubulin gene of resistant isolates were identified. Among 31 resistant isolates, the frequency of point mutation types in F167Y, E198Q, and E198L of the β-tubulin gene was 71.0, 25.8, and 3.2%, respectively. The data indicate that has developed resistance to carbendazim in Henan Province, and single point mutations at amino acid F167Y were the predominant type of mutation detected.
Topics: Benzimidazoles; Carbamates; Drug Resistance, Fungal; Fungicides, Industrial; Fusarium; Genes, Plant; Point Mutation; Triticum; Tubulin
PubMed: 31424998
DOI: 10.1094/PDIS-02-19-0391-RE -
Food Chemistry Sep 2021A method for simultaneous determination of carbendazim and tebuconazole residues in cabbage was developed and validated in LC-MS/MS. Samples were extracted and purified...
A method for simultaneous determination of carbendazim and tebuconazole residues in cabbage was developed and validated in LC-MS/MS. Samples were extracted and purified following the modified QuEChERS procedure, which enabled the elution of carbendazim and tebuconazole at 0.96 and 5.31 min, respectively. LOD and LOQ were 0.0005 and 0.0015 mg kg, respectively. Mean recovery was in the range of 78.94 to 104.89% for carbendazim and 76.07 to 98.62% for tebuconazole. The field samples recorded residues of 0.274 and 0.481 mg kg; and 0.194 and 0.392 mg kg at single and double dose for carbendazim and tebuconazole, respectively. Half-life values were 2.17 and 2.99 for carbendazim and 2.74 and 2.81 for tebuconazole at single and double dose, respectively. Decontamination with saltwater wash followed by cooking and lemon water wash found superior in the removal of residues more than 90%.
Topics: Benzimidazoles; Brassica; Carbamates; Chromatography, Liquid; Decontamination; Food Analysis; Food Contamination; Half-Life; Pesticide Residues; Tandem Mass Spectrometry; Time Factors; Triazoles
PubMed: 33780796
DOI: 10.1016/j.foodchem.2021.129523 -
Chemosphere Apr 2022Residues from multiple pesticides are frequently detected on vegetables, which may produce combined toxicity not predicted by individual toxicity data. As these combined...
Residues from multiple pesticides are frequently detected on vegetables, which may produce combined toxicity not predicted by individual toxicity data. As these combined effects present additional dangers to food safety, we have compared individual to combined effects for a variety of pesticides. Carbendazim and chlorpyrifos are the two most commonly detected pesticides in vegetables, and previous studies reported that combined exposure results in synergistic developmental toxicity to zebrafish embryos. In this study, individual and combined effects on zebrafish motor activity were examined following individual and combined exposure to assess nervous system toxicity. Further, transcriptomics methods were used to identify potential molecular mechanisms for individual and combined toxicity. Carbendazim alone induced a disorganized swim pattern characterized by increased angular velocity, turn angle, meander, and acceleration during light-dark transition, while chlorpyrifos alone reduced average swim speed and light-dark acceleration. Combined treatment significantly reduced average swim velocity and total distance traveled. Combination indices indicated strong antagonism between compounds for average speed and light-dark acceleration. Transcriptomics (RNA-seq) showed that carbendazim significantly altered the expression of genes involved in antigen processing and presentation, apoptosis, autophagy, and metabolism, including ctslb, cyp7a1, hsp70l, and ugt1a1. Alternatively, chlorpyrifos significantly altered genes involved in various nervous system-related pathways, including glutamatergic, GABAergic, dopaminergic, and calcium signaling. Protein-protein interaction (PPI) network analysis suggested that chlorpyrifos significantly downregulated genes related to light transduction, resulting in decreased sensitivity to light-dark transitions, while antagonism mainly reflected divergent effects on phototransduction and retinol metabolism. Carbendazim had no significant effects on vision-related genes such as gnat1 and gngt1, while chlorpyrifos downregulated expression, an effect reversed by the combination. Comprehensive toxicity analyses must include joint effects of co-applied pesticides for enhanced food safety.
Topics: Animals; Benzimidazoles; Carbamates; Chlorpyrifos; Larva; Pesticides; Zebrafish
PubMed: 34995633
DOI: 10.1016/j.chemosphere.2022.133522 -
Journal of Biochemical and Molecular... Aug 2022Carbendazim (CBZ) is a common environmental pollutant that can contaminate food and water and severely damage human health. Some studies revealed the adverse effect of...
Carbendazim (CBZ) is a common environmental pollutant that can contaminate food and water and severely damage human health. Some studies revealed the adverse effect of CBZ on different organs, but its detailed toxicity mechanism has not been elucidated yet. Thus, the present study aims to clarify the mechanisms of CBZ-induced hepatorenal toxicity in rats. Therefore, we partitioned 40 male Wistar rats into four groups (n = 10): a negative control group and three treatment groups, which received 100, 300, and 600 mg/kg of CBZ. All rats received the treatment daily by oral gavage. We collected blood and organ samples (liver and kidney) at 14 and 28 days postdosing. CBZ caused extensive pathological alterations in both the liver and kidneys, such as cellular degeneration and necrosis accompanied by severe inflammatory reactions in a dose- and time-dependent manner. All the CBZ-treated groups displayed strong tumor necrosis factor-α and nuclear factor-κB (NF-κB) immunopositivity. Additionally, CBZ dose-dependently elevated the alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, urea, and creatinine serum levels and reduced the serum albumin levels. Furthermore, CBZ-induced apoptosis, as indicated by the observed Bax gene upregulation and Bcl-2 gene downregulation in both organs. All these changes may be related to oxidative stress, as indicated by the increase in malondialdehyde levels and the decrease in total antioxidant capacity. Our results demonstrate that CBZ-induced dose- and time-dependent hepatorenal damage through oxidative stress, which activated both the NF-κB signaling pathway and Bcl-based programmed cell death.
Topics: Animals; Antioxidants; Benzimidazoles; Carbamates; Chemical and Drug Induced Liver Injury; Kidney; Liver; Male; NF-kappa B; Oxidative Stress; Rats; Rats, Wistar; Signal Transduction
PubMed: 35437878
DOI: 10.1002/jbt.23079 -
Chemosphere Jun 2022Agriculture is having a major role in solving issues associated with food shortages across the globe. Carbendazim (CZM) is one of the fungicides which is commonly used...
Agriculture is having a major role in solving issues associated with food shortages across the globe. Carbendazim (CZM) is one of the fungicides which is commonly used in agriculture to grow crops in large quantities and fast. Monitoring CZM content is in high demand for environmental remediation. The present work deals with the synthesis of gadolinium sesquisulfide anchored Nitrogen-doped reduced graphene oxide (GdS/NRGO) through a simple microwave-assisted method. X-ray diffraction and morphological studies confirm the formation of the nanocomposite. GdS/NRGO showed enhanced activity both in electrochemical detection and light-driven degradation of CZM compared to GdS and NRGO. GdS/NRGO modified glassy carbon electrode (GCE) exhibit a wide linear range of 0.01-450 μM CZM with 0.009 μM LOD using differential pulse voltammetry (DPV). GdS/NRGO@GCE showed good selectivity, stability, and recovery (98.13-99.10%) in the river water sample. In addition, GdS/NRGO has been explored towards the visible-light-induced degradation of CZM. The reactions conditions were optimized to achieve maximum efficiency. 94% of CZM was degraded within 90 min in presence of GdS/NRGO. Mechanism of electrochemical redox reaction and degradation of CZM in presence of GdS/NRGO has been explored to the maximum extent possible. Degradation intermediates were identified using LC-MS.
Topics: Benzimidazoles; Carbamates; Carbon; Electrochemical Techniques; Gadolinium; Graphite
PubMed: 35189195
DOI: 10.1016/j.chemosphere.2022.134030