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British Medical Journal Sep 1978
Topics: Humans; Methoxsalen; Photochemotherapy; Psoriasis
PubMed: 698683
DOI: 10.1136/bmj.2.6138.703-c -
The New Zealand Medical Journal Aug 1979Photochemotherapy for psoriasis using ordinary fluorescent blacklight combined with orally administered methoxalen was used in the treatment of 20 patients suffering...
Photochemotherapy for psoriasis using ordinary fluorescent blacklight combined with orally administered methoxalen was used in the treatment of 20 patients suffering from long standing psoriasis. Ten of the patients had complete clearing of their lesions, two had 75 percent or more clearance, one patient had 50 percent improvement and there was only slight improvement in two further cases. Side effects were minimal.
Topics: Administration, Oral; Adolescent; Erythema; Female; Humans; Methoxsalen; Nausea; Photochemotherapy; Pruritus; Psoriasis
PubMed: 290909
DOI: No ID Found -
Annals of Plastic Surgery May 1985Psoralen therapy followed by exposure to long-wave ultraviolet (UV-A) light, a combination called PUVA therapy, stimulates pigmentation of the skin. PUVA therapy is...
Psoralen therapy followed by exposure to long-wave ultraviolet (UV-A) light, a combination called PUVA therapy, stimulates pigmentation of the skin. PUVA therapy is effective in the treatment of vitiligo and hypopigmented scars. Because psoralens sensitize skin to UV-A light, phototoxic reactions are frequent. Patients with such reactions can present to the plastic surgeon because they clinically mimic thermal burns. We report a patient with vitiligo who treated herself with oral and topical psoralens and then exposed herself to sunlight. She developed an intense, burnlike reaction, with erythema, edema, and bullae. "Sunburn" may be a major injury in the psoralen user.
Topics: Adult; Female; Humans; Methoxsalen; PUVA Therapy; Photosensitivity Disorders; Substance-Related Disorders; Sunlight; Vitiligo
PubMed: 4083703
DOI: 10.1097/00000637-198505000-00010 -
The New England Journal of Medicine Dec 1974
Topics: Cell Division; DNA; Humans; Methoxsalen; Psoriasis; Skin; Ultraviolet Therapy
PubMed: 4423112
DOI: 10.1056/NEJM197412052912312 -
Journal of Chromatography May 1993An improved method suitable for the determination of 8-methoxypsoralen in the range 50-1500 ng/ml in the plasma of psoriatic patients undergoing PUVA (psoralens and...
An improved method suitable for the determination of 8-methoxypsoralen in the range 50-1500 ng/ml in the plasma of psoriatic patients undergoing PUVA (psoralens and long-wave ultraviolet light) therapy is proposed. A 5-ml aliquot of plasma containing sodium citrate as anticoagulant was centrifuged, griseofulvin was added as internal standard and the sample was denatured with acetonitrile. The supernatant was applied to C18 cartridges and 8-methoxypsoralen was eluted with methanol. The evaporated eluate was reconstituted in the mobile phase for high-performance liquid chromatography (HPLC) and applied to the HPLC column: mobile phase, acetonitrile-0.01 M phosphoric acid (34:66); flow-rate, 1 ml/min; temperature, 40 degrees C; column, Spherisorb 5 ODS, 100 mm x 4.6 mm I.D., 5 microns particle size; UV detection at 248 nm; detection limit, 15 ng/ml of plasma.
Topics: Chromatography, High Pressure Liquid; Griseofulvin; Humans; Methoxsalen; PUVA Therapy; Psoriasis
PubMed: 8314948
DOI: No ID Found -
Photo-dermatology Jun 1984A selected ion-monitoring gas chromatographic mass spectrometric method is described for the determination of methoxsalen employing trioxsalen as internal standard....
A selected ion-monitoring gas chromatographic mass spectrometric method is described for the determination of methoxsalen employing trioxsalen as internal standard. Methoxsalen in the serum and skin of 5 psoriatic patients after PUVA bath therapy was determined. Quantitative gas chromatographic mass spectrometric analysis was performed in the election impact mode by monitoring the ratio of ions m/z 216/228. The methoxsalen level in human serum varied between 3-15 ng/ml, in suction blister fluid between 2-9 ng/ml and in suction blister roof (epidermis) between 6-242 ng/g during the first 30 min after the methoxsalen bath.
Topics: Blister; Gas Chromatography-Mass Spectrometry; Humans; Methoxsalen; Skin
PubMed: 6527955
DOI: No ID Found -
Journal of AOAC International Jan 2018The possibility of following traditional cold-press extraction with the post process continuous separation of bergapten from bergamot essential oil was investigated. A...
The possibility of following traditional cold-press extraction with the post process continuous separation of bergapten from bergamot essential oil was investigated. A fractionation tower was used in an experiment in which cold-pressed bergamot oil was extracted in a continuous countercurrent process by supercritical carbon dioxide under different conditions. Bergapten is fairly soluble in CO2 in its supercritical phase, in particular at a density of 277.90 kg⋅m-3, corresponding to a pressure of 8 MPa and temperature of 40°C. Under these conditions, an extract with 0.198% bergapten was obtained, a figure slightly below the percentage of bergapten contained in cold-pressed oil (0.21%). However, at densities below 200 kg⋅m-3, the amount of bergapten in the extracted oil was negligible. Of all tested conditions for separation, the best was found to be at a pressure of 8 MPa and temperature of 70°C, conditions under which bergapten was not detected. The results of the experiment showed that bergapten, and the non-volatile fraction in general, was extracted only in small quantities and was not extracted at all with at a CO2 pressure of 8 MPa.
Topics: 5-Methoxypsoralen; Carbon Dioxide; Methoxsalen; Oils, Volatile; Plant Oils
PubMed: 28762330
DOI: 10.5740/jaoacint.17-0190 -
Journal of the American Academy of... Jun 1997After oral intake, 5-methoxypsoralen (5-MOP) is as effective as 8-MOP for PUVA therapy for psoriasis, with a lower incidence of acute cutaneous side effects. (Comparative Study)
Comparative Study
BACKGROUND
After oral intake, 5-methoxypsoralen (5-MOP) is as effective as 8-MOP for PUVA therapy for psoriasis, with a lower incidence of acute cutaneous side effects.
OBJECTIVE
We compared bath-water delivery of 5-MOP and 8-MOP for photochemotherapy of psoriasis.
METHODS
Twenty-two patients underwent phototesting with 0.0003% 5-MOP or 8-MOP aqueous solutions. Twelve patients with palmar psoriasis were studied with a side-to-side comparison, and 10 patients with recurrent plaque-type psoriasis were treated with one therapy or the other.
RESULTS
Minimal phototoxic dose (MPD) values were 2.8 +/- 1.2 J/cm2 with 8-MOP and 2.0 +/- 1.2 J/cm2 with 5-MOP (p < 0.01). Both therapies cleared palmar lesions but 8-MOP required more UVA irradiation (46.3 +/- 21.0 J/cm2 vs 30.2 +/- 21.5 J/cm2; p < 0.01) and more exposures (21.0 +/- 6.0 vs 17.0 +/- 5.0; p = 0.02). Bath-5-MOP-UVA was also more effective in the treatment of plaque-type psoriasis (cumulative UVA doses, 56.8 +/- 39.2 vs 59.1 +/- 27.9 J/cm2; number of exposures, 20.0 +/- 5.7 vs 21.6 +/- 4.7), but these differences were not significant (p = NS). Patients developed an intense tan significantly earlier with 5-MOP than with 8-MOP (3.5 +/- 0.5 weeks vs 4.4 +/- 0.5 weeks; p < 0.01).
CONCLUSION
Bath-5-MOP-UVA was more phototoxic than bath-8-MOP-UVA. It was more effective in the treatment of palmar psoriasis, whereas its greater pigmentogenic activity appeared to have an adverse effect on therapeutic effectiveness in the treatment of plaque-type psoriasis.
Topics: 5-Methoxypsoralen; Administration, Oral; Adult; Aged; Baths; Female; Humans; Male; Methoxsalen; Middle Aged; PUVA Therapy; Photosensitizing Agents; Psoriasis
PubMed: 9204060
DOI: 10.1016/s0190-9622(97)80278-2 -
Brain Research Bulletin Dec 2020The ever-present trend for introducing new drugs of natural origin with anxiolytic properties meets healthcare needs of the population, whose almost 34 % struggles with... (Comparative Study)
Comparative Study
The ever-present trend for introducing new drugs of natural origin with anxiolytic properties meets healthcare needs of the population, whose almost 34 % struggles with anxiety-related disorders. At the same time, animal assays that could serve as fast and reliable models of anxiety-like behaviors are of great interest to scientists. Thus, the aim of the present study was to evaluate the utility of the zebrafish model for assessing the influence of natural compounds on anxiety in comparison with the well-known mouse model. Secondly, this study is also the first attempt to investigate the influence of a naturally occurring metabolite, i.e. xanthotoxin, on anxiety-related behaviors. The anxiety level in zebrafish was assessed by measuring thigmotaxis, a specific animal behavior to move closer to the boundaries of an open area and to avoid its center. In mice, the elevated plus maze test was chosen to study anxiety-related behaviors. Our results show that xanthotoxin exerted reversed U-shape effect on anxiety behaviors in both models. The similar pattern of xanthotoxin-induced anxiety-related behaviors in both animal models not only confirms the pharmacological properties of xanthotoxin but also proves the predictive power of the zebrafish model for behavioral research of natural compounds.
Topics: Animals; Anti-Anxiety Agents; Anxiety; Behavior, Animal; Disease Models, Animal; Male; Methoxsalen; Mice; Motor Activity; Zebrafish
PubMed: 33049351
DOI: 10.1016/j.brainresbull.2020.09.024 -
European Journal of Medicinal Chemistry Sep 2010A series of graveoline and graveolinine derivatives were synthesized. The biological results showed that most of graveoline derivatives possessed higher cytotoxicity and...
A series of graveoline and graveolinine derivatives were synthesized. The biological results showed that most of graveoline derivatives possessed higher cytotoxicity and better inhibitive effect against the adhesion and migration of human umbilical vein endothelial cell (HUVEC) than graveolinine derivatives. Among these compounds, 8d was the most potent agents that also showed significant anti-angiogenesis activities in chick embryo chorioallantoic membrane (CAM) assay.
Topics: Angiogenesis Inhibitors; Animals; Cell Adhesion; Cell Line, Tumor; Cell Movement; Chick Embryo; Chorioallantoic Membrane; Endothelial Cells; Humans; Methoxsalen; Quinolones
PubMed: 20554355
DOI: 10.1016/j.ejmech.2010.05.043