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Transplant Infectious Disease : An... Jun 2017We report the first case of Mycoplasma hominis periaortic abscess after heart-lung transplantation. The absence of sternal wound infection delayed the diagnosis, but the...
We report the first case of Mycoplasma hominis periaortic abscess after heart-lung transplantation. The absence of sternal wound infection delayed the diagnosis, but the patient successfully recovered with debridement surgeries and long-term antibiotic therapy. Owing to the difficulty in detection and the intrinsic resistance to beta-lactams, M. hominis infections are prone to being misdiagnosed and undertreated. M. hominis should be suspected in cases where conventional microbiological identification and treatment approaches fail.
Topics: Abscess; Adult; Anti-Bacterial Agents; Bronchoscopy; Cardiomyopathy, Restrictive; Debridement; Dyspnea; Glucocorticoids; Graft Rejection; Heart-Lung Transplantation; Humans; Hypertension, Pulmonary; Levofloxacin; Male; Methylprednisolone; Minocycline; Mycoplasma hominis; Nausea; Plasmapheresis; Postoperative Complications; Respiratory Insufficiency; Sternum; Surgical Wound Infection; Tomography, X-Ray Computed; Young Adult; beta-Lactam Resistance; beta-Lactamases
PubMed: 28294481
DOI: 10.1111/tid.12697 -
BMJ Case Reports Nov 2022is a rare but important cause of prosthetic valve endocarditis. It is usually associated with acute progression of symptoms and can be difficult to diagnose as it does...
is a rare but important cause of prosthetic valve endocarditis. It is usually associated with acute progression of symptoms and can be difficult to diagnose as it does not grow in standard culture media. We report a case of an immunocompetent man in his 70s who presented with 14-month subacute decline with shortness of breath and evidence of a splenic infarct. Following a redo aortic valve replacement and diagnosis of through 16S ribosomal ribonucleic acid PCR, he improved clinically with oral doxycycline therapy. He remained well at follow-up 2 years post-cessation of antibiotics. We present a literature review highlighting the role of PCR testing in the microbiological identification of .
Topics: Male; Humans; Mycoplasma hominis; Endocarditis, Bacterial; Heart Valve Prosthesis; Mycoplasma Infections; Endocarditis
PubMed: 36319037
DOI: 10.1136/bcr-2022-252972 -
JBJS Case Connector May 2021A 63-year-old man underwent L2-S1 decompression and fusion for spinal stenosis. He developed urinary retention postoperatively requiring catheterization. He developed...
CASE
A 63-year-old man underwent L2-S1 decompression and fusion for spinal stenosis. He developed urinary retention postoperatively requiring catheterization. He developed fever, purulence, and foot-drop 8 days postoperatively and underwent debridement with implant retention. Cultures yielded Mycoplasma hominis after 10 days. He received 4 weeks of doxycycline. Four years postoperatively, he had no recurrence of infection and was able to ambulate despite a persistent foot-drop.
CONCLUSION
Mycoplasma hominis is a urogenital commensal rarely implicated in musculoskeletal infections. A high index of suspicion is required in spinal surgery patients who develop fever and purulence and have initial negative cultures and poor response to empirical antibiotics.
Topics: Decompression; Humans; Lumbosacral Region; Male; Middle Aged; Mycoplasma hominis; Spinal Stenosis; Wound Infection
PubMed: 33950867
DOI: 10.2106/JBJS.CC.20.00439 -
The Journal of Molecular Diagnostics :... Nov 2023Bacterial commensals of the human genitourinary tract, Mycoplasma hominis and Ureaplasma species (parvum and urealyticum) can be sexually transmitted, and may cause...
Bacterial commensals of the human genitourinary tract, Mycoplasma hominis and Ureaplasma species (parvum and urealyticum) can be sexually transmitted, and may cause nongonococcal urethritis, pelvic inflammatory disease, and infertility. Mycoplasma hominis and Ureaplasma species may also cause severe invasive infections in immunocompromised patients. Current culture-based methods for Mycoplasma/Ureaplasma identification are costly and laborious, with a turnaround time between 1 and 2 weeks. We developed a high-throughput, real-time multiplex PCR assay for the rapid detection of M. hominis and Ureaplasma species in urine, genital swab, body fluid, and tissue. In total, 282 specimens were tested by PCR and compared with historic culture results; a molecular reference method was used to moderate discrepancies. Overall result agreement was 99% for M. hominis (97% positive percentage agreement and 100% negative percentage agreement) and 96% for Ureaplasma species (96% positive percentage agreement and 97% negative percentage agreement). Specimen stability was validated for up to 7 days at room temperature. This multiplex molecular assay was designed for implementation in a high-complexity clinical microbiology laboratory. With this method, >90 samples can be tested in one run, with a turnaround time of 4 to 5 hours from specimen extraction to reporting of results. This PCR test is also more labor effective and cheaper than the conventional culture-based test, thus improving laboratory efficiency and alleviating labor shortages.
Topics: Mycoplasma hominis; Humans; Ureaplasma; Multiplex Polymerase Chain Reaction; Real-Time Polymerase Chain Reaction; Ureaplasma Infections; Mycoplasma Infections; Sensitivity and Specificity; High-Throughput Screening Assays; DNA, Bacterial; Reproducibility of Results
PubMed: 37683891
DOI: 10.1016/j.jmoldx.2023.07.004 -
Bulletin of Experimental Biology and... Aug 2021Culturing of Mycoplasma hominis in the presence of arginine and thymidine and subsequent comparative proteomic analysis of cells showed that, in addition to the already... (Comparative Study)
Comparative Study
Culturing of Mycoplasma hominis in the presence of arginine and thymidine and subsequent comparative proteomic analysis of cells showed that, in addition to the already known arginine dihydrolase pathway of energy metabolism, M. hominis can utilize deoxyribose phosphates formed as a result of catabolism of pyrimidine nucleosides. In this case, a sharp deceleration of cell growth was observed. This allows M. hominis to occupy new niches in the host organism and survive under competitive conditions when the main sources of energy are unavailable.
Topics: Arginine; Bacteriological Techniques; Carbon; Culture Media; Electrophoresis, Gel, Two-Dimensional; Humans; Mycoplasma Infections; Mycoplasma hominis; Proteome; Proteomics; Thymidine
PubMed: 34542749
DOI: 10.1007/s10517-021-05247-8 -
BMC Microbiology May 2022To explore the impact of pre-pregnancy vaginal Mycoplasma hominis (M. hominis) colonization of low abundance on female fecundability.
OBJECTIVE
To explore the impact of pre-pregnancy vaginal Mycoplasma hominis (M. hominis) colonization of low abundance on female fecundability.
METHODS
In total, 89 females participating in a pre-pregnancy health examination program were included, and their pregnancy outcomes were followed up for 1 year. Vaginal swabs were collected, 16S rRNA genes were sequenced, and M. hominis colonization was confirmed by qPCR. Cox models were used to estimate the fecundability odds ratio (FOR) for women with M. hominis.
RESULTS
The prevalence of M. hominis was 22.47% (20/89), and the abundance was relatively low (the cycle thresholds of the qPCR were all more than 25). In terms of the vaginal microbiome, the Simpson index of the positive group was significantly lower than that of the negative group (P = 0.003), which means that the microbiome diversity appeared to increase with M. hominis positivity. The relative abundance of M. hominis was negatively correlated with Lactobacillus crispatus (rho = - 0.24, P = 0.024), but positively correlated with Gardnerella vaginalis, Atopobium vaginae and Prevotella bivia (P all < 0.05). The cumulative one-year pregnancy rate for the M. hominis positive group was lower than that in the negative group (58.96% vs 66.76%, log-rank test: P = 0.029). After controlling for potential confounders, the risk of pregnancy in the M. hominis positive group was reduced by 38% when compared with the positive group (FOR = 0.62, 95% CI: 0.42-0.93).
CONCLUSION
The vaginal colonization of M. hominis at a low level in pre-pregnant women is negatively correlated with female fecundability.
Topics: Cohort Studies; Female; Fertility; Gardnerella vaginalis; Humans; Male; Mycoplasma hominis; Pregnancy; RNA, Ribosomal, 16S; Vagina; Vaginosis, Bacterial
PubMed: 35513786
DOI: 10.1186/s12866-022-02545-7 -
Transplant Infectious Disease : An... Oct 2020A 42-year-old man presented with nausea, malaise, and pain at his renal graft site 4 months following deceased donor renal transplant. His transplantation had been... (Review)
Review
A 42-year-old man presented with nausea, malaise, and pain at his renal graft site 4 months following deceased donor renal transplant. His transplantation had been complicated by urinary leak with delayed wound closure requiring ureteral revision with biologic mesh placement. The initial evaluation in the hospital revealed urinalysis with significant pyuria as well as abdominal CT imaging concerning for abscess formation anterior to the grafted kidney. Interventional radiology (IR) guided drainage of this abscess yielded growth of Enterococcus faecalis treated with intravenous ampicillin/sulbactam. He continued to have pain at his graft site and repeat imaging revealed a persistent abscess despite prolonged antimicrobial therapy. Urine cultures isolated Mycoplasma species. A repeat aspirate of abscess fluid collected and Mycoplasma hominis was identified by molecular test. Patient's symptoms abated and his abscess completely resolved on repeat imaging after completing a course of oral moxifloxacin and doxycycline. His immunosuppression did not require adjustment and the renal graft continued to function well following this therapy. Mycoplasma and Ureaplasma should be considered as a potential etiology for perinephric abscess in renal transplant recipients.
Topics: Abscess; Adult; Humans; Kidney; Kidney Transplantation; Male; Mycoplasma hominis; Urinary Tract Infections
PubMed: 32378787
DOI: 10.1111/tid.13308 -
The Pediatric Infectious Disease Journal Dec 2002Scalp abscess in the newborn is usually a complication of fetal scalp monitoring and is typically polymicrobial, reflecting maternal vaginal flora. We present a newborn... (Review)
Review
Scalp abscess in the newborn is usually a complication of fetal scalp monitoring and is typically polymicrobial, reflecting maternal vaginal flora. We present a newborn with no apparent scalp trauma who developed scalp abscess that yielded pure growth of Mycoplasma hominis. The clue to diagnosis was the growth of pinpoint translucent colonies on blood agar that were Gram stain-negative. Abscess drainage and systemic clindamycin were curative.
Topics: Abscess; Clindamycin; Combined Modality Therapy; Drainage; Follow-Up Studies; Humans; Infant, Newborn; Infusions, Intravenous; Male; Mycoplasma Infections; Mycoplasma hominis; Risk Assessment; Scalp; Treatment Outcome
PubMed: 12506953
DOI: 10.1097/00006454-200212000-00020 -
BMJ Case Reports Jul 2021
Topics: Abscess; Cesarean Section; Female; Humans; Mycoplasma Infections; Mycoplasma hominis; Pregnancy; Pregnancy Complications, Infectious
PubMed: 34330730
DOI: 10.1136/bcr-2021-244128 -
Frontiers in Cellular and Infection... 2022is an opportunistic urogenital pathogen in vertebrates. It is a non-glycolytic species that produces energy arginine degradation. Among genital mycoplasmas, is the...
is an opportunistic urogenital pathogen in vertebrates. It is a non-glycolytic species that produces energy arginine degradation. Among genital mycoplasmas, is the most commonly reported to play a role in systemic infections and can persist in the host for a long time. However, it is unclear how proceeds under arginine limitation. The recent metabolic reconstruction of has demonstrated its ability to catabolize deoxyribose phosphate to produce ATP. In this study, we cultivated on two different energy sources (arginine and thymidine) and demonstrated the differences in growth rate, antibiotic sensitivity, and biofilm formation. Using label-free quantitative proteomics, we compared the proteome of under these conditions. A total of 466 proteins were identified from , representing approximately 85% of the predicted proteome, while the levels of 94 proteins changed significantly. As expected, we observed changes in the levels of metabolic enzymes. The energy source strongly affects the synthesis of enzymes related to RNA modifications and ribosome assembly. The translocation of lipoproteins and other membrane-associated proteins was also impaired. Our study, the first global characterization of the proteomic switching of in arginine-deficiency media, illustrates energy source-dependent control of pathogenicity factors and can help to determine the mechanisms underlying the interaction between the growth rate and fitness of genome-reduced bacteria.
Topics: Arginine; Lipoproteins; Mycoplasma hominis; Proteome; Proteomics
PubMed: 35873139
DOI: 10.3389/fcimb.2022.918557