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The Journal of Infection Jul 2005Mycoplasma hominis and Ureaplasma urealyticum are associated with various diseases of the urogenital tract, but they are usually not detected by routine microbiological...
OBJECTIVES
Mycoplasma hominis and Ureaplasma urealyticum are associated with various diseases of the urogenital tract, but they are usually not detected by routine microbiological diagnosis. The aim of this study was to asses the prevalence of these organisms in patients with sterile pyuria.
METHODS
From December 2000 to June 2001 all urine samples sent in to microbiological diagnosis, which yielded > or =500 leucocytes/ml and <10(4) bacteria/ml, were collected for this study. Thirty-three samples from 30 patients (female: 21, male: nine; median age: 60 years, range: 23-91 years) met these criteria and were subjected to PCR for detection of M. hominis and U. urealyticum, respectively.
RESULTS
M. hominis and U. urealyticum were detected in 2 (7%) and 6 samples (20%), respectively. With regard to the underlying diseases of the patients, glomerulonephritis was significantly associated with the detection of urogenital mycoplasmas (p=0.02).
CONCLUSION
The prevalence of M. hominis and U. urealyticum found in this study corresponds to the expected prevalence in the general population. This finding does not indicate an association of sterile pyuria with urogenital mycoplasma infection/colonization.
Topics: Adult; Aged; Aged, 80 and over; Female; Humans; Male; Middle Aged; Mycoplasma Infections; Mycoplasma hominis; Prevalence; Pyuria; Ureaplasma Infections; Ureaplasma urealyticum
PubMed: 15979492
DOI: 10.1016/j.jinf.2004.06.010 -
Current Microbiology Sep 2022Mutations in some miRNAs are associated with human recurrent pregnancy loss (RPL). In parallel, Mycoplasma spp. are one of the most common infections in pregnant women....
Mutations in some miRNAs are associated with human recurrent pregnancy loss (RPL). In parallel, Mycoplasma spp. are one of the most common infections in pregnant women. The objective of this study was to identify the relationship between miRNA196a-2 gene polymorphism and Mycoplasma hominis (M. hominis) infection as a possible cause of human abortion. A total of 160 cervical swab specimens were collected from women (80 samples with at least one abortion as case, and 80 samples without abortion as control). A PCR-based method using 16S rRNA gene and tetra primer amplification refractory mutation system-polymerase chain (Tetra-ARMS-PCR) were used to identify the presence of M. hominis infections and miRNA196a-2 genotypes of studied women, respectively. Results showed that 22.5% of women with abortion and 7.5% of women without abortion were infected with M. hominis, thereby suggesting a significant difference between the two groups (P < 0.05). Tetra-ARMSPCR indicated that no significant difference in frequency of genotypes existed between women experimenting abortion and control group. Independently to the presence of M. hominis infection, a significant difference (P < 0.05) was observed in genotypic frequencies of miRNA196a-2 between RPL women and those with one abortion. Estimation of the Odds Ratios indicated that the chance of recurrent abortions in TT genotypes of miRNA196a-2 was about three times more likely than CC in non-infected individuals and about five times more likely than CC in M. hominis-infected patients. Our results proposed the role of miRNA196a-2 genotypes in RPL either in M. hominis-infected or non-infected individuals.
Topics: Female; Humans; MicroRNAs; Mycoplasma Infections; Mycoplasma hominis; Polymorphism, Single Nucleotide; Pregnancy; RNA, Ribosomal, 16S
PubMed: 36155856
DOI: 10.1007/s00284-022-03034-w -
Revista Espanola de Cardiologia... Nov 2015
Topics: Angina Pectoris, Variant; Anti-Bacterial Agents; Aortic Valve Insufficiency; Aortic Valve Stenosis; Echocardiography, Transesophageal; Endocarditis, Bacterial; Heart Valve Prosthesis; Heart Valve Prosthesis Implantation; Humans; Male; Middle Aged; Mycoplasma Infections; Mycoplasma hominis; Prosthesis Failure; Prosthesis-Related Infections; RNA, Ribosomal, 16S
PubMed: 26481285
DOI: 10.1016/j.rec.2015.07.018 -
Research in Microbiology 2017Mycoplasma hominis was isolated in 1937 from the human genital tract, followed 17 years later by Ureaplasma urealyticum and 27 years after that by Mycoplasma genitalium....
Mycoplasma hominis was isolated in 1937 from the human genital tract, followed 17 years later by Ureaplasma urealyticum and 27 years after that by Mycoplasma genitalium. The first two proved relatively easy to culture but the latter required a polymerase chain reaction assay for further studies. In sexually mature women, M. hominis may be found in the vagina/cervix of about 20-50%, ureaplasmas in 40-80% and M. genitalium in 0-5%. Some heterogeneity has been found among strains of all these species, sufficient to divide ureaplasmas into two species, namely U. urealyticum and Ureaplasma parvum. Studies in female mice show that sex hormones have a profound influence on colonization, multiplication and persistence of mycoplasmas/ureaplasmas in the genital tract and provoke the question, unanswered, of whether there is such an effect in the human tract. In women, there is no evidence that any of the mycoplasmal species stimulate an inflammatory vaginitis. M. hominis organisms increase hugely in number in the case of bacterial vaginosis (BV), and to a lesser extent so do ureaplasmas. Despite this, they have not been incriminated as a sole cause of BV. Evidence for the involvement of M. genitalium remains controversial. The strong association of BV with preterm birth raises the possibility that the genital mycoplasmas might play a part, but assurance that any do will be difficult to obtain. Detailed examination of the vaginal microbiome has not yet provided an answer.
Topics: Animals; Cervix Uteri; Female; Humans; Mice; Microbiota; Mycoplasma genitalium; Mycoplasma hominis; Pregnancy; Pregnancy Complications, Infectious; Ureaplasma; Ureaplasma urealyticum; Vagina; Vaginosis, Bacterial
PubMed: 28263902
DOI: 10.1016/j.resmic.2017.02.009 -
The Journal of Cardiovascular Surgery Oct 1996Wound infections with Mycoplasma species are unusual; diagnosis may be delayed because of the growth characteristics of this organism. We report Mycoplasma hominis... (Review)
Review
Wound infections with Mycoplasma species are unusual; diagnosis may be delayed because of the growth characteristics of this organism. We report Mycoplasma hominis infection of sternotomy wounds in two patients. The first presented with fever and drainage from the incision 1 week after coronary artery bypass grafting. The other patient presented with drainage from the incision three weeks after double-lung transplantation. In both cases, initial cultures were negative, but the typical colonial morphology of M. hominis was subsequently detected. Successful treatment consisted of debridement and long courses of antibiotic therapy; omental flap grafting was eventually required for the second patient. Other published cases were reviewed and compared with the newly reported cases.
Topics: Aged; Coronary Artery Bypass; Fatal Outcome; Humans; Lung Transplantation; Male; Mycoplasma Infections; Mycoplasma hominis; Sternum; Surgical Wound Infection
PubMed: 8941693
DOI: No ID Found -
The Journal of International Medical... Oct 2021The prevalence of and (genital mycoplasma) amongst Indonesian women is poorly understood because of limited availability of diagnostic techniques. We sought to compare... (Observational Study)
Observational Study
OBJECTIVE
The prevalence of and (genital mycoplasma) amongst Indonesian women is poorly understood because of limited availability of diagnostic techniques. We sought to compare the diagnostic parameters of the AF Genital System® with those of culture methods and PCR as the gold standard for identification of and in vaginal swab specimens.
METHODS
This was an observational diagnostic study. Eighty-eight specimens were collected from patients with abnormal vaginal discharge. Detection of and was performed using the AF Genital System®, culture methods, and PCR.
RESULTS
Compared with PCR and culture methods, respectively, the AF Genital System® had sensitivities of 66.6% and 57% () and 55.5% and 77.8% (). Compared with PCR and culture methods, respectively, the AF Genital System® had specificities of 82.9% and 86.5% () and 82.3% and 84.8% ().
CONCLUSION
The sensitivity of the AF Genital System® for detection of and from vaginal swab samples was lower than that of PCR, but specificity was reasonably good (82% to 83%).
Topics: Female; Humans; Mycoplasma Infections; Mycoplasma hominis; Ureaplasma Infections; Ureaplasma urealyticum; Vagina
PubMed: 34686092
DOI: 10.1177/03000605211053278 -
Archives of Iranian Medicine Jun 2015It has been recognized that infectious agents, such as different bacteria and viruses, may play a role in the developing of rheumatoid arthritis (RA). Recently, the...
BACKGROUND
It has been recognized that infectious agents, such as different bacteria and viruses, may play a role in the developing of rheumatoid arthritis (RA). Recently, the mycoplasma species has been implicated in the pathogenesis of RA.
AIM
The aim of this study was to design a multiplex PCR for rapid and simultaneous detection of Mycoplasma pneumoniae, Mycoplasma hominis, and Mycoplasma arthritidis in the synovial fluid of patients with rheumatoid arthritis (RA).
METHODS
A total of 131 synovial fluid (SF) samples from patients with RA were assayed. Mycoplasma pneumoniae (ATCC: 29342), M. hominis (native strain), and the synthetic complete genome of M. arthritidis mitogen (MAM) superantigen were used as controls. All SF samples were subjected to DNA extraction separately and multiplex PCR was performed. The PCR products were confirmed by sequencing.
RESULTS
The designed multiplex PCR was able to detect M. pneumoniae, M. hominis, and M. arthritidis in the SF of patients with RA with a frequency of 30 (22.9%), 23 (17.5%) and 13 (9.9%), respectively.
CONCLUSION
In this study, the overall detection of the Mycoplasma species in RA patients was 53.4%; thus, we recommend the application of multiplex PCR assays when searching for a specific anti mycoplasma treatment for RA patients.
Topics: Arthritis, Rheumatoid; Female; Humans; Male; Middle Aged; Multiplex Polymerase Chain Reaction; Mycoplasma arthritidis; Mycoplasma hominis; Mycoplasma pneumoniae; Sensitivity and Specificity; Sequence Analysis; Synovial Fluid
PubMed: 26058928
DOI: No ID Found -
Wounds : a Compendium of Clinical... Dec 2020Thoracic aortic graft infection is a rare complication after cardiac surgery with no consensus having been reached on the most appropriate type of management. Although...
INTRODUCTION
Thoracic aortic graft infection is a rare complication after cardiac surgery with no consensus having been reached on the most appropriate type of management. Although most commonly caused by gram-positive cocci, aortic graft infection by Mycoplasma hominis can occur.
CASE REPORT
A 63-year-old male patient with an aneurysm of the ascending aorta was admitted to the authors' institution for surgical treatment. A replacement of the ascending aorta with a polyester vascular graft was performed using a valve-sparing aortic valve reimplantation technique. During postoperative course, the patient became septic with a spiking fever and elevated inflammatory markers. A computed tomography scan revealed signs of mediastinitis. M hominis was identified in blood cultures and intraoperative tissue samples. An antibiogram-based antibiotic solution (tigecycline in 5% glucose solution) was instilled twice daily into the thoracic cavity using negative pressure wound therapy with instillation and dwell time for 8 days. This type of management allowed the authors to avoid graft replacement, and good midterm outcomes were achieved.
CONCLUSIONS
To the authors' best knowledge, this is the first described successful treatment of an aortic graft infection caused by M hominis without a surgical removal of the prosthetic material using antibiotic irrigation.
Topics: Aorta; Aorta, Thoracic; Blood Vessel Prosthesis; Humans; Male; Middle Aged; Mycoplasma hominis; Negative-Pressure Wound Therapy
PubMed: 33476287
DOI: No ID Found -
Experimental Parasitology Jul 2012Trichomonas vaginalis can be naturally infected with intracellular Mycoplasma hominis. This bacterial infection may have implications for trichomonal virulence and...
Trichomonas vaginalis can be naturally infected with intracellular Mycoplasma hominis. This bacterial infection may have implications for trichomonal virulence and disease pathogenesis. The objective of the study was to report the presence of M. hominis in Cuban T. vaginalis isolates and to describe the association between the phenotype M. hominis infected with RAPD genetic polymorphism of T. vaginalis. The Random Amplified Polymorphic DNA (RAPD) technique was used to determine genetic differences among 40 isolates of T. vaginalis using a panel of 30 random primers and these genetic data were correlated with the infection of isolates with M. hominis. The trees drawn based on RAPD data showed no relations with metronidazole susceptibility and significantly association with the presence of M. hominis (P=0.043), which demonstrates the existence of concordance between the genetic relatedness and the presence of M. hominis in T. vaginalis isolates. This result could point to a predisposition of T. vaginalis for the bacterial enters and/or survival.
Topics: Cuba; DNA, Bacterial; Female; Humans; Multiplex Polymerase Chain Reaction; Mycoplasma hominis; Phylogeny; Polymorphism, Genetic; Random Amplified Polymorphic DNA Technique; Tenericutes; Trichomonas vaginalis; Urogenital System
PubMed: 22584035
DOI: 10.1016/j.exppara.2012.05.003 -
Polish Journal of Microbiology Dec 2021is associated with various infections, for which the treatment can be complex. Lipoic acid (LA) plays a role as a cofactor in eukaryotes, most Bacteria, and some...
is associated with various infections, for which the treatment can be complex. Lipoic acid (LA) plays a role as a cofactor in eukaryotes, most Bacteria, and some Archea. Research of recent years has increasingly pointed to the therapeutic properties of exogenously supplemented LA. The present study was conducted on 40 strains of cultured with the following LA concentrations: 1,200 μg/ml, 120 μg/ml, and 12 μg/ml. The bacterial colonies of each strain were counted and expressed as the number of colony-forming units/ml (CFU). The number of CFU in strains obtained in the presence of LA was compared with the number of CFU in the strains grown in the media without LA. The obtained results indicated that the presence of LA in the medium did not affect the growth of . The investigation of the influence of LA on the growth and survival of microbial cells not only allows for obtaining an answer to the question of whether LA has antimicrobial activity and, therefore, can be used as a drug supporting the treatment of patients infected with a given pathogenic microorganism. Such studies are also crucial for a better understanding of LA metabolism in the microbial cells, which is also important for the search for new antimicrobial drugs. This research is, therefore, an introduction to such further studies.
Topics: Anti-Bacterial Agents; Humans; Microbial Sensitivity Tests; Mycoplasma Infections; Mycoplasma hominis; Thioctic Acid
PubMed: 35003281
DOI: 10.33073/pjm-2021-050