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Revista Espanola de Quimioterapia :... Dec 2022
Topics: Humans; Flavobacteriaceae; Urinary Tract Infections
PubMed: 36183239
DOI: 10.37201/req/045.2022 -
Scandinavian Journal of Infectious... 2001A case of Myroides odoratus cellulitis with bacteremia in an apparently immunocompetent man is presented. Although common in soil and water, this agent is a rare... (Review)
Review
A case of Myroides odoratus cellulitis with bacteremia in an apparently immunocompetent man is presented. Although common in soil and water, this agent is a rare clinical isolate and is often not considered pathogenic. The virulence of M. odoratus may be greater than is currently believed and it should be considered in bacteremias from cutaneous sources in immunocompetent patients.
Topics: Adult; Aged; Anti-Bacterial Agents; Cellulitis; Female; Flavobacterium; Gram-Negative Bacterial Infections; Humans; Immunocompromised Host; Infant; Male; Middle Aged
PubMed: 11868770
DOI: 10.1080/00365540110077065 -
Infection Oct 2014Myroides sp., previously known as Flavobacterium odoratum, is a relatively unknown organism with unclear human pathogenicity. While Myroides sp. has been implicated in... (Review)
Review
Myroides sp., previously known as Flavobacterium odoratum, is a relatively unknown organism with unclear human pathogenicity. While Myroides sp. has been implicated in human infections, many reports have described the organism as a relatively avirulent opportunistic pathogen. We present an unusual case of rapidly fatal necrotizing fasciitis and septic shock due to Myroides odoratus. Our case demonstrates the pathogenicity of Myroides, and highlights potential risk factors for infection including underlying liver disease and open wounds. The recognition of Myroides is of particular importance given its resistance to multiple antibiotics. We review the literature on Myroides sp. skin and soft tissue infections, including necrotizing forms, and discuss the clinical presentation and management of this potentially emerging pathogen.
Topics: Anti-Bacterial Agents; California; Diagnosis, Differential; Fasciitis, Necrotizing; Fatal Outcome; Female; Flavobacteriaceae; Flavobacteriaceae Infections; Humans; Microbial Sensitivity Tests; Middle Aged; Shock, Septic
PubMed: 24806817
DOI: 10.1007/s15010-014-0626-0 -
Case Reports in Infectious Diseases 2022The bacterial genus , like other members of the family, consists of aerobic, non-motile, Gram-negative bacilli. spp. is considered predominantly opportunistic...
The bacterial genus , like other members of the family, consists of aerobic, non-motile, Gram-negative bacilli. spp. is considered predominantly opportunistic pathogens as, historically, most documented infections have been in immunocompromised individuals. Along with advancements in molecular assay testing, there are growing reports of clinically relevant spp. infections in immunocompetent individuals. These organisms display broad antimicrobial resistance, and while research into their mechanisms of resistance is progressing, genetic testing has revealed metallo--lactamases present in their genome. The sporadic identification of spp. and ongoing clarification of resistance patterns make empiric treatment difficult. This report documents two cases of extensively drug-resistant isolated from critically ill but otherwise immunocompetent patients followed by a review of available literature on spp. antibiotic sensitivities. Our findings indicate that minocycline and moxifloxacin have the highest documented activity against spp.
PubMed: 35873406
DOI: 10.1155/2022/6422861 -
Diagnostic Microbiology and Infectious... Jun 2014The genus Myroides comprises the 2 medically relevant species Myroides odoratus and Myroides odoratimimus that are rare opportunistic pathogens and cause infections in... (Comparative Study)
Comparative Study
The genus Myroides comprises the 2 medically relevant species Myroides odoratus and Myroides odoratimimus that are rare opportunistic pathogens and cause infections in immunocompromised patients. A fast identification of Myroides is of importance because these bacterial strains show multiple resistance against antibiotics and therefore limit treatment options. They are associated, for instance, with urinary tract infections, sepsis, meningitis, pneumonia, and infectious cellulitis. Since more and more Myroides spp. are being described, additional potentially pathogenic bacteria may be identified in the future demanding the need for fast and reliable identification methods at species level. However, to date, only molecular approaches meet these demands. In this study, we, therefore, attempt to define an appropriate method other than DNA fingerprinting that will permit a comparable efficacy and, possibly, a more economical strain identification. For this purpose, we compared 2 widely used automated diagnostic systems (VITEK 2 [bioMérieux, Nürtingen, Germany] and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) [Bruker Daltonics, Bremen, Germany]) and correlated the results to 16S rDNA sequencing data. In total, we analyzed 22 strains collected in the course of routine diagnostics. In this study, we demonstrate that VITEK 2 reliably identifies the genus Myroides but cannot differentiate between M. odoratimimus and M. odoratus. In contrast to this, both MALDI-TOF MS and 16S rDNA sequencing efficiently distinguish between the 2 species.
Topics: Bacterial Typing Techniques; DNA, Ribosomal; Flavobacteriaceae; Flavobacteriaceae Infections; Humans; Molecular Diagnostic Techniques; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 24666701
DOI: 10.1016/j.diagmicrobio.2014.02.002 -
Emerging Microbes & Infections Apr 2018The genus Myroides comprises several species of Gram-negative, non-motile, and non-fermenting bacteria, which have been regarded as non-pathogenic for decades. Multiple...
The genus Myroides comprises several species of Gram-negative, non-motile, and non-fermenting bacteria, which have been regarded as non-pathogenic for decades. Multiple recent reports, however, underscore the pathogenic potential that Myroides sp. possesses for humans. These bacteria seem to be resistant to a wide range of antibiotics (including ß-lactams and aminoglycosides). Therefore, treatment options are limited. Knowledge of antimicrobial resistance, however, is based on only one meaningful comprehensive study and on data published from case reports. This lack of data motivated us to test 59 strains from our Myroides collection (43 M. odoratimimus and 16 M. odoratus) for resistance against 20 commonly used antibiotics. We also performed molecular analyses to reveal whether our bacteria harbor the genus-specific M. odoratimimus metallo-ß-lactamase (MUS-1) or the M. odoratus metallo ß-lactamase (TUS-1), and other ß-lactamases, which may provide an explanation for the extended antimicrobial resistance.
Topics: Anti-Bacterial Agents; DNA, Bacterial; Flavobacteriaceae; Humans; Microbial Sensitivity Tests; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Whole Genome Sequencing; beta-Lactamases
PubMed: 29618738
DOI: 10.1038/s41426-018-0061-x -
Microbial Drug Resistance (Larchmont,... Sep 2018Myroides odoratus is a low-virulence opportunistic human pathogen. Infections caused by M. odoratus are not common, but reports are increasing in recent years. The...
Myroides odoratus is a low-virulence opportunistic human pathogen. Infections caused by M. odoratus are not common, but reports are increasing in recent years. The biggest challenge for treatment is its resistance to most antibiotics. In 2015, we isolated a pathogenic multidrug-resistant strain of M. odoratus from a urinary tract infection (UTI) patient's urine sample. To report the experience in managing M. odoratus-related UTI and investigate the genetic mechanism of this carbapenem-resistant strain, we conducted a series of microbiological and molecular studies. The bacterial strain was identified as M. odoratus by 16S rRNA gene sequencing. The minimum inhibitory concentrations (MICs) of 17 antimicrobial agents were determined against this strain. Whole-genome sequencing was performed and screened for possible β-lactamase genes. A β-lactamase gene, bla, was identified by whole-genome sequencing, then cloned and expressed in Escherichia coli DH5α to characterize its function. Antimicrobial susceptibility testing showed that the strain had relatively low MIC to levofloxacin and sulfamethoxazole/trimethoprim (SMZ/TMP). After unsuccessful empirical therapy, the UTI was controlled by levofloxacin in combination with SMZ/TMP. Whole-genome sequencing identified a subclass B1 metallo-β-lactamase gene, bla, which conferred resistance to most β-lactams except for aztreonam and cefepime. In conclusion, a new β-lactamase gene, bla, was found in an isolate of M. odoratus. The broad antimicrobial resistance of this isolate is at least attributed partially to this gene.
Topics: Amino Acid Sequence; Anti-Bacterial Agents; Carbapenems; Drug Resistance, Multiple, Bacterial; Escherichia coli; Flavobacteriaceae; Humans; Microbial Sensitivity Tests; RNA, Ribosomal, 16S; Sequence Alignment; Urinary Tract Infections; Whole Genome Sequencing; beta-Lactamases; beta-Lactams
PubMed: 29227741
DOI: 10.1089/mdr.2017.0233 -
Antonie Van Leeuwenhoek Jan 2015A Gram-negative, yellow-pigmented, rod-shaped bacteria, designated M09-0166(T)and M09-1053 were isolated from human urine samples. 16S rRNA gene sequence analysis...
A Gram-negative, yellow-pigmented, rod-shaped bacteria, designated M09-0166(T)and M09-1053 were isolated from human urine samples. 16S rRNA gene sequence analysis revealed that the isolates belong to the Myroides cluster and were closely related to Myroides phaeus DSM 23313(T) (96.3 %), Myroides odoratimimus KCTC 23053(T) (96.1 %), Myroides profundi KCTC 23066(T) (96.0 %), Myroides odoratus KCTC 23054(T) (95.4 %) and Myroides pelagicus KCTC 12661(T) (95.2 %). The major mena quinone was identified as MK-6. The major polar lipids were identified as phosphatidylethanolamine, amino lipids, and several unknown lipids, and the major fatty acids as iso-C15:0 and iso-C17:0 3-OH. Phenotypic and chemotaxonomic data supported the affiliation of the isolates with the genus Myroides and clearly indicated that two isolates represent novel species, for which the name Myroides injenensis sp. nov. (type strain, M09-0166(T) = KCTC 23367(T) = JCM 17451(T)) is proposed.
Topics: Bacterial Typing Techniques; Cluster Analysis; Cytosol; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Flavobacteriaceae; Flavobacteriaceae Infections; Humans; Molecular Sequence Data; Phospholipids; Phylogeny; Pigments, Biological; Quinones; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Urine
PubMed: 25515411
DOI: 10.1007/s10482-014-0317-y -
International Journal of Systematic and... Nov 2015A novel aerobic, non-motile, rod-shaped, catalase- and oxidase-positive bacterial strain, designated UKS3T, was isolated from garden soil, and subjected to polyphasic...
A novel aerobic, non-motile, rod-shaped, catalase- and oxidase-positive bacterial strain, designated UKS3T, was isolated from garden soil, and subjected to polyphasic taxonomic analysis. Strain UKS3T formed whitish, viscous colonies on nutrient agar and was Gram-staining negative. Phylogenetic analysis, based on 16S rRNA gene sequence, showed that maximum pairwise similarity occurs with representatives of the genus Myroides. The most closely related species include Myroides marinus JS-08T (92.7 % sequence similarity), Myroides phaeus MY15T (92.7 %), Myroides odoratus DSM 2801T (91.5 %) and Myroides odoratimimus CCUG 39352T (91.4 %). Strain UKS3T contained menaquinone-6 (MK-6) as the major respiratory quinone and iso-C15 : 0 (40.2 %), anteiso-C15 : 0 (9.4 %) and iso-C17 : 0 3-OH (8.5 %) as major fatty acids. Phosphatidylethanolamine, phospholipids and three aminolipids were the major polar lipids. The DNA G+C content of strain UKS3T was 36.8 ± 2.0 mol%. On the basis of phenotypic, chemotaxonomic and molecular analysis, strain UKS3T represents a novel species of the genus Myroides, for which the name Myroides indicus sp. nov., is proposed. The type strain is UKS3T ( = DSM 28213T = NCIM 5555T ).
Topics: Bacteria, Aerobic; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Flavobacteriaceae; Gardens; India; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Vitamin K 2
PubMed: 26276159
DOI: 10.1099/ijsem.0.000530 -
Indian Journal of Medical Microbiology 2014Myroides spp and Chryseobacterium spp are uncommon clinical isolates, though more frequently reported to cause infections than other pigmented non-fermentors. Two cases...
Myroides spp and Chryseobacterium spp are uncommon clinical isolates, though more frequently reported to cause infections than other pigmented non-fermentors. Two cases of Myroides odoratus and Chryseobacterium indologenes infection in a diabetic with pulmonary tuberculosis and a patient with de-compensated alcoholic liver disease, respectively, are reported here. Anti-microbial susceptibility testing of the isolates was performed by determining the minimum inhibitory concentration. The clinical picture, characteristic features of the isolates and the antibiotic susceptibility pattern are discussed briefly.
Topics: Anti-Bacterial Agents; Chryseobacterium; Female; Flavobacteriaceae; Flavobacteriaceae Infections; Humans; Immunocompromised Host; Male; Microbial Sensitivity Tests; Middle Aged
PubMed: 25008832
DOI: 10.4103/0255-0857.136592