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Biomedicine & Pharmacotherapy =... May 2022Panax notoginseng (Burkill) F.H. commonly referred to as Sanqi, is a Chinese herb that has long been used to treat various conditions including blood disorders and...
INTRODUCTION
Panax notoginseng (Burkill) F.H. commonly referred to as Sanqi, is a Chinese herb that has long been used to treat various conditions including blood disorders and cardiovascular diseases. While Panax notoginseng has been used as an anti-cancer medicinal herb in recent years, how it achieves this therapeutic effect has not been thoroughly elucidated. The purpose of this study was to reveal more about the mechanism of the cytotoxic effect of Panax notoginseng on prostate cancer (PCa) cells.
METHODS
Ethanol extract of Panax notoginseng root was authenticated using high-performance liquid chromatography (HPLC). The cytotoxic activity of this herb against PCa cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, flow cytometry, and enzyme-linked immunosorbent assay (ELISA).
RESULTS
The assessment of cellular metabolic activity demonstrated that Panax notoginseng reduces the viability of LNCaP and 22Rv1 cells in a dose-dependent manner. Annexin-V binding flow cytometry assay showed that Panax notoginseng induces apoptosis in PCa cells. Cell cycle analysis by quantification of DNA content using flow cytometry showed that Panax notoginseng arrests the cell cycle at the G2/M phase in both LNCaP and 22Rv1 cells. Moreover, ELISA demonstrated that Panax notoginseng-treated PCa cells secrete significantly less tumor-promoting cytokine interleukin-4 (IL-4) to the supernatant compared with controls.
CONCLUSIONS
These results provide evidence for the cytotoxic effects of Panax notoginseng on PCa cell lines. This botanical is a promising candidate for the complementary and integrative medicine treatment of PCa and further studies are indicated to determine the anti-cancer mechanism of Panax notoginseng.
Topics: Antineoplastic Agents; Chromatography, High Pressure Liquid; Humans; Male; Panax; Panax notoginseng; Plants, Medicinal; Prostatic Neoplasms; Saponins
PubMed: 35367754
DOI: 10.1016/j.biopha.2022.112887 -
Zhongguo Zhong Yao Za Zhi = Zhongguo... Mar 2013The authors reviewed the new technologies used for Panax genus research, including molecular identification technologies (especially for DNA barcoding), modern... (Review)
Review
The authors reviewed the new technologies used for Panax genus research, including molecular identification technologies (especially for DNA barcoding), modern biotechnologies (e. g. the first generation and second generation sequencing technologies), and gene cloning and identification in this paper. These technologies have been successfully applied to species identification, transcriptome analysis, secondary metabolite biosynthetic pathway and the key enzyme function identification, indicating that the application of modern biotechnologies provide guarantee for the molecular identification of Panax genus. The application of modern biotechnologies also reveals the genetic information of transcriptome and functional genomics, and promotes the design of Panax plants genomic map. In summary, the application of the new technologies lay the foundation for clarifying the molecular mechanisms of ginsenoside biosynthesis and enforcing the in vitro synthesis of important natural products and new drugs in future.
Topics: Biotechnology; Cloning, Molecular; DNA Fingerprinting; Ginsenosides; Panax; Research Design
PubMed: 23724665
DOI: No ID Found -
Molecules (Basel, Switzerland) Sep 2019Recently has been grown as a secondary crop under a pine tree canopy in New Zealand (NZ). The aim of the study is to compare the average content of ginsenosides from...
Recently has been grown as a secondary crop under a pine tree canopy in New Zealand (NZ). The aim of the study is to compare the average content of ginsenosides from NZ-grown ginseng and its original native locations (China and Korea) grown ginseng. Ten batches of NZ-grown ginseng were extracted using 70% methanol and analyzed using LC-MS/MS. The average content of ginsenosides from China and Korea grown ginseng were obtained by collecting data from 30 and 17 publications featuring China and Korea grown ginseng, respectively. The average content of total ginsenosides in NZ-grown ginseng was 40.06 ± 3.21 mg/g (n = 14), which showed significantly ( < 0.05) higher concentration than that of China grown ginseng (16.48 ± 1.24 mg/g, n = 113) and Korea grown ginseng (21.05 ± 1.57 mg/g, n = 106). For the individual ginsenosides, except for the ginsenosides Rb2, Rc, and Rd, ginsenosides Rb1, Re, Rf, and Rg1 from NZ-grown ginseng were 2.22, 2.91, 1.65, and 1.27 times higher than that of ginseng grown in China, respectively. Ginsenosides Re and Rg1 in NZ-grown ginseng were also 2.14 and 1.63 times higher than ginseng grown in Korea. From the accumulation of ginsenosides, New Zealand volcanic pumice soil may be more suitable for ginseng growth than its place of origin.
Topics: China; Geography; Ginsenosides; Molecular Structure; New Zealand; Panax; Republic of Korea
PubMed: 31561496
DOI: 10.3390/molecules24193491 -
Journal of Pharmaceutical and... Nov 2022An efficient supercritical fluid chromatography-mass spectrometry (SFC-MS) method was developed for the quality evaluation of Panax Notoginseng (Burk) F.H. Chen (P....
An efficient supercritical fluid chromatography-mass spectrometry (SFC-MS) method was developed for the quality evaluation of Panax Notoginseng (Burk) F.H. Chen (P. notoginseng) by combination with chemical pattern recognition (CPR). Design of experiments (DoE) was applied to obtain optimal SFC-MS conditions. Several CPR methods including hierarchical cluster analysis (HCA), principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were employed to establish a classification model based on the peak areas and contents of 12 components in P. notoginseng in order to evaluate the quality difference according to the collecting time (Chunqi and Dongqi) and medicinal parts (fibrous root, rhizome, branch root, and main root). PLS-DA has proved to be a satisfactory method with accurate discrimination of the selected samples. The characteristic variables based on the variable importance in projection (VIP) values were selected using PLS-DA. Three characteristic components (ginsenoside Rg, ginsenoside Rg, ginsenoside Rb) with higher VIP values (>1) were chosen to further build the CPR model. Subsequently, the model was verified by testing another set of samples and the results indicated that the established model was satisfactory. PLS-DA models based on the peak areas of the 12 selected analytes in 30 batches of P. notoginseng could give accurate classification. The obtained results demonstrate that the developed method using SFC-MS and PLS-DA has a great potential for the quality assessment of P. notoginseng.
Topics: Chromatography, High Pressure Liquid; Chromatography, Supercritical Fluid; Ginsenosides; Mass Spectrometry; Panax; Panax notoginseng; Rhizome
PubMed: 36162277
DOI: 10.1016/j.jpba.2022.115029 -
Planta Medica Jan 2011Ginsengs (Panax, Araliaceae) are among the plants best known for their medicinal properties. Many ginseng species are endangered due to over-exploitation of natural...
Ginsengs (Panax, Araliaceae) are among the plants best known for their medicinal properties. Many ginseng species are endangered due to over-exploitation of natural resources - a situation difficult to remedy while there are no reliable, practical methods for species identification. We screened eleven candidate DNA barcoding loci to establish an accurate and effective Panax species identification system, both for commercial and conservation purposes. We used 95 ginseng samples, representing all the species in the genus. We found considerable differences in the performance of the potential barcoding regions. The sequencing of ATPF-ATPH was unsuccessful due to poly-N structures. The RBCL, RPOB, and RPOC1 regions were found to be mostly invariable, with only four to eight variable sites. Using MATK, PSBK-I, PSBM-TRND, RPS16 and NAD1, we could identify four to six out of eight considerably divergent species but only one to five out of nineteen clusters within the P. bipinnatifidus species group. PSBA-TRNH and ITS were the most variable loci, working very well both in species and cluster identifications. We demonstrated that the combination of PSBA-TRNH and ITS is sufficient for identifying all the species and clusters in the genus.
Topics: Base Sequence; DNA Barcoding, Taxonomic; DNA, Plant; Electronic Data Processing; Genetic Loci; Genetic Variation; Molecular Sequence Data; Panax; Phylogeny; Polymerase Chain Reaction; Sequence Analysis, DNA
PubMed: 20803416
DOI: 10.1055/s-0030-1250166 -
Journal of Chromatography. A Jul 2022To comprehensively elucidate the herbal metabolites is crucial in natural products research to discover new lead compounds. Ginsenosides are an important class of...
An off-line three-dimensional liquid chromatography/Q-Orbitrap mass spectrometry approach enabling the discovery of 1561 potentially unknown ginsenosides from the flower buds of Panax ginseng, Panax quinquefolius and Panax notoginseng.
To comprehensively elucidate the herbal metabolites is crucial in natural products research to discover new lead compounds. Ginsenosides are an important class of bioactive components from the Panax plants exerting the significant tonifying effects. However, to identify new ginsenosides by the conventional strategies trends to be more and more difficult because of the large spans of acid-base property (the neutral and acidic saponins), molecular mass (400-1400 Da), and rather low content. Herein, an off-line multidimensional chromatography/high-resolution mass spectrometry approach was presented: ion exchange chromatography (IEC) as the first dimension of separation, hydrophilic interaction chromatography (HILIC) in the second dimension, and reversed-phase chromatography (RPC) for the third dimension which was hyphenated to a Q Exactive Q-Orbitrap mass spectrometer. By applying to the flower buds of P. ginseng (PGF), P. quinquefolius (PQF), and P. notoginseng (PNF), IEC using a PhenoSphere SAX column could fractionate the total extracts into the neutral (unretained) and acidic (retained) fractions, while HILIC (an XBridge Amide column) and RPC (BEH Shield RP18 column) achieved the hydrophilic interaction and hydrophobic interaction separations, respectively. Q-Orbitrap mass spectrometry offered rich structural information and complementary resolution to the co-eluting components, particular to those minor ones by including precursor ion lists in data-dependent acquisition. We could characterize 803 ginsenosides from PGF, 795 from PQF, and 833 from PNF, and 1561 thereof are potentially unknown. These results can indicate the great potential of this multidimensional approach in the ultra-deep characterization of complex herbal samples supporting the efficient discovery of potentially novel natural compounds.
Topics: Chromatography, High Pressure Liquid; Chromatography, Liquid; Flowers; Ginsenosides; Mass Spectrometry; Panax; Panax notoginseng
PubMed: 35660315
DOI: 10.1016/j.chroma.2022.463177 -
Current Pharmaceutical Biotechnology 2021Ginseng, also known as the king of herbs, has been regarded as an important traditional medicine for several millennia. Ginsenosides, a group of triterpenoid saponins,... (Review)
Review
Ginseng, also known as the king of herbs, has been regarded as an important traditional medicine for several millennia. Ginsenosides, a group of triterpenoid saponins, have been characterized as bioactive compounds of ginseng. The complexity of ginsenosides hindered ginseng research and development both in cultivation and clinical research. Therefore, deciphering the ginsenoside biosynthesis pathway has been a focus of interest for researchers worldwide. The new emergence of biological research tools consisting of omics and bioinformatic tools or computational biology tools are the research trend in the new century. Ginseng is one of the main subjects analyzed using these new quantification tools, including tools of genomics, transcriptomics, and proteomics. Here, we review the current progress of ginseng omics research and provide results for the ginsenoside biosynthesis pathway. Organization and expression of the entire pathway, including the upstream MVA pathway, the cyclization of ginsenoside precursors, and the glycosylation process, are illustrated. Regulatory gene families such as transcriptional factors and transporters are also discussed in this review.
Topics: Animals; Ginsenosides; Humans; Panax; Transcriptome
PubMed: 32767915
DOI: 10.2174/1389201021666200807113723 -
Food Chemistry Sep 2023An efficient decolorization method for ginseng residue oligosaccharides (GROs) using ultrasound-assisted D392 macroporous resin was developed. The decolorization effects...
An efficient decolorization method for ginseng residue oligosaccharides (GROs) using ultrasound-assisted D392 macroporous resin was developed. The decolorization effects and color differences of activated carbon adsorption, hydrogen peroxide oxidation, and resin adsorption were evaluated. The optimal conditions of the three decolorization methods for static, dynamic, ultrasound-assisted resin adsorption were compared. The results showed that ultrasound-assisted decolorization had the best decolorization effect of greatly decreasing the decolorization time to 80 min. Color difference analysis revealed the process of pigment removal during GRO decolorization. The UV-visible full-wavelength scan showed that most pigments were removed after decolorization. The characterizations by the Fourier-transform infrared spectroscopy and X-ray diffraction analysis showed that the chemical structure and crystallinity of the GROs did not change upon decolorization. In addition, the molecular weight distribution did not change significantly. This research contributes to further exploration of the structures and functions of GROs.
Topics: Porosity; Panax; Ultrasonics; Oligosaccharides; Color
PubMed: 37031538
DOI: 10.1016/j.foodchem.2023.136098 -
Molecular Ecology Sep 2023Genomic structural variations (SVs) are widespread in plant and animal genomes and play important roles in phenotypic novelty and species adaptation. Frequent whole...
Genomic structural variations (SVs) are widespread in plant and animal genomes and play important roles in phenotypic novelty and species adaptation. Frequent whole genome duplications followed by (re)diploidizations have resulted in high diversity of genome architecture among extant species. In this study, we identified abundant genomic SVs in the Panax genus that are hypothesized to have occurred through during the repeated polyploidizations/(re)diploidizations. Our genome-wide comparisons demonstrated that although these polyploidization-derived SVs have evolved at distinct evolutionary stages, a large number of SV-intersecting genes showed enrichment in functionally important pathways related to secondary metabolites, photosynthesis and basic cellular activities. In line with these observations, our metabolic analyses of these Panax species revealed high diversity of primary and secondary metabolites both at the tissue and interspecific levels. In particular, genomic SVs identified at ginsenoside biosynthesis genes, including copy number variation and large fragment deletion, appear to have played important roles in the evolution and diversification of ginsenosides. A further herbivore deterrence experiment demonstrated that, as major triterpenoidal saponins found exclusively in Panax, ginsenosides provide protection against insect herbivores. Our study provides new insights on how polyploidization-derived SVs have contributed to phenotypic novelty and plant adaptation.
Topics: Ginsenosides; Panax; DNA Copy Number Variations; Saponins; Adaptation, Physiological
PubMed: 37525516
DOI: 10.1111/mec.17088 -
Current Pharmaceutical Design 2017Ginseng has gained fame as one of the most popular herbs originating from Eastern countries. Among different species which are known as ginseng, Panax ginseng C. A. Mey.... (Review)
Review
Ginseng has gained fame as one of the most popular herbs originating from Eastern countries. Among different species which are known as ginseng, Panax ginseng C. A. Mey. (Korean or Asian ginseng) is the most frequently used one. Ginsenosides have been proposed to account for most of the biological activities of ginseng. The widely appreciated health-promoting effect of ginseng pertains to the beneficial effects of this plant against immune, cardiovascular and sexual diseases and cancer. In addition, there are some new aspects of the pharmacological activity of this plant which justify its use in dermatologic diseases. In dermatology, ginseng has been investigated mechanistically for its therapeutic effects in photoaging, wound and injury, skin cancer, dermatitis, hair loss, alopecia and cold hypersensitivity. Here, we reviewed experimental and clinical studies exploring the therapeutic efficacy of ginseng and ginsenosides in the field of dermatology.
Topics: Cell Proliferation; Dermatology; Ginsenosides; Humans; Panax; Skin Diseases
PubMed: 27774902
DOI: 10.2174/1381612822666161021152322