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Journal of Clinical Microbiology Mar 2017We designed a universal human papillomavirus (HPV) typing assay based on target enrichment and whole-genome sequencing (eWGS). The RNA bait included 23,941 probes...
We designed a universal human papillomavirus (HPV) typing assay based on target enrichment and whole-genome sequencing (eWGS). The RNA bait included 23,941 probes targeting 191 HPV types and 12 probes targeting beta-globin as a control. We used the Agilent SureSelect XT2 protocol for library preparation, Illumina HiSeq 2500 for sequencing, and CLC Genomics Workbench for sequence analysis. Mapping stringency for type assignment was determined based on 8 (6 HPV-positive and 2 HPV-negative) control samples. Using the optimal mapping conditions, types were assigned to 24 blinded samples. eWGS results were 100% concordant with Linear Array (LA) genotyping results for 9 plasmid samples and fully or partially concordant for 9 of the 15 cervical-vaginal samples, with 95.83% overall type-specific concordance for LA genotyping. eWGS identified 7 HPV types not included in the LA genotyping. Since this method does not involve degenerate primers targeting HPV genomic regions, PCR bias in genotype detection is minimized. With further refinements aimed at reducing cost and increasing throughput, this first application of eWGS for universal HPV typing could be a useful method to elucidate HPV epidemiology.
Topics: Genome, Viral; Genomics; Genotype; Genotyping Techniques; Humans; Papillomaviridae; Papillomavirus Infections; Sequence Analysis, DNA
PubMed: 27974548
DOI: 10.1128/JCM.02132-16 -
The Journal of Infectious Diseases Jan 2017In Australia, high uptake of the quadrivalent human papillomavirus (4vHPV) vaccine has led to reductions in the prevalence of human papillomavirus (HPV) genotypes 6, 11,...
BACKGROUND
In Australia, high uptake of the quadrivalent human papillomavirus (4vHPV) vaccine has led to reductions in the prevalence of human papillomavirus (HPV) genotypes 6, 11, 16, and 18 in women and girls aged ≤25 years. We evaluated the impact of the program impact on HPV prevalence in unvaccinated male subjects.
METHODS
Sexually active heterosexual male subjects aged 16-35 years were recruited in 2014-2016. Participants provided a self-collected penile swab sample for HPV genotyping (Roche Linear Array) and completed a demographic and risk factor questionnaire.
RESULTS
The prevalence of 4vHPV genotypes among 511 unvaccinated male subjects was significantly lower in those aged ≤25 than in those aged >25 years: 3.1% (95% confidence interval, 1.5%-5.7%) versus 13.7% (8.9%-20.1%), respectively (P < .001); adjusted prevalence ratio, 0.22 (.09-.51; P < .001). By contrast, the prevalence of high-risk HPV genotypes other than 16 and 18 remained the same across age groups: 16.8% (95% confidence interval, 12.6%-21.9%) in men aged ≤25 years and 17.9% (12.4%-25.0%) in those aged >25 years (P = .76); adjusted prevalence ratio, 0.98, (.57-1.37; P = .58).
CONCLUSIONS
A 78% lower prevalence of 4vHPV genotypes was observed among younger male subjects. These data suggest that unvaccinated men may have benefited from herd protection as much as women from a female-only HPV vaccination program with high coverage.
Topics: Adolescent; Adult; Australia; Female; Heterosexuality; Humans; Immunization Programs; Male; Papillomaviridae; Papillomavirus Infections; Penis; Prevalence; Surveys and Questionnaires; Young Adult
PubMed: 27815379
DOI: 10.1093/infdis/jiw530 -
Journal of Virology Nov 1989The cloning and partial characterization of the genome of human papillomavirus (HPV) type 48 is presented. Hybridization and short DNA sequence analyses permitted the...
The cloning and partial characterization of the genome of human papillomavirus (HPV) type 48 is presented. Hybridization and short DNA sequence analyses permitted the alignment of the genome to the HPV genetic map.
Topics: Carcinoma, Squamous Cell; DNA, Viral; Genes, Viral; Humans; Nucleic Acid Hybridization; Papillomaviridae; Restriction Mapping; Skin Neoplasms
PubMed: 2552166
DOI: 10.1128/JVI.63.11.4907-4908.1989 -
Comparative Immunology, Microbiology... Dec 2020Papillomaviruses affect both human and non-human hosts. In camels, papillomatosis is caused by Camelus dromedarius papillomavirus type 1 and 2 (CdPV1 and CdPV2,...
Papillomaviruses affect both human and non-human hosts. In camels, papillomatosis is caused by Camelus dromedarius papillomavirus type 1 and 2 (CdPV1 and CdPV2, respectively). In late 2018, an outbreak of camelpox occurred in a herd of fattening camels in Egypt. Several animals were found to be co-infected with camelpox and camel papillomaviruses. The morbidity with papillomatosis was 35 %. The infection was confirmed by PCR then Illumina sequencing revealed the presence of a complete genome of two CdPVs. One of these was CdPV1 (MT130101) and the other was a putative novel virus, tentatively named as CdPV3 (MT130100). Seven ORFs and a long upstream regulatory region were identified in the genomes of both viruses. Pairwise comparisons of L1 gene revealed 98.92 % nt identity between MT130101/CdPV1/Egypt/2018 and HQ912790/CdPV1/Sudan/2009 with 100 % coverage. However, MT130100/CdPV3/ Egypt/2018 showed only 68.99 % nt identity with the closest genome HQ912791/CdPV2/Sudan/2009. Phylogenetic analyses indicated that CdPV1 and CdPV3 belonged to the genus Deltapapillomavirus. These results should be useful for future CdPVs molecular surveillance and construction of evolutionary characteristics of this virus.
Topics: Animals; Camelus; Disease Outbreaks; Egypt; Molecular Sequence Annotation; Papillomaviridae; Papillomavirus Infections; Phylogeny; Polymerase Chain Reaction
PubMed: 33091861
DOI: 10.1016/j.cimid.2020.101561 -
Nederlands Tijdschrift Voor Geneeskunde May 1989
Review
Topics: Carcinoma, Squamous Cell; Female; Genes, Viral; Humans; Papillomaviridae; Proto-Oncogene Proteins; Suppression, Genetic; Tumor Virus Infections; Uterine Neoplasms
PubMed: 2544813
DOI: No ID Found -
Microbiological Reviews Jun 1982
Review
Topics: Animals; Antigens, Viral; Biological Evolution; Bovine papillomavirus 1; Cell Transformation, Viral; Cells, Cultured; Cottontail rabbit papillomavirus; Genes, Viral; Humans; Papilloma; Papillomaviridae; Recombination, Genetic; Transcription, Genetic; Tumor Virus Infections
PubMed: 6289064
DOI: 10.1128/mr.46.2.191-207.1982 -
Journal of Medical Virology Feb 2014Cervical cancer is the most frequent cancer among women in Senegal. However, there are few data concerning the human papillomavirus (HPV) types inducing neoplasia and...
Cervical cancer is the most frequent cancer among women in Senegal. However, there are few data concerning the human papillomavirus (HPV) types inducing neoplasia and cervical cancers and their prevalence in the general population of Senegal. The aim of this study is to determine the prevalence of HPV infection in Senegalese women aged 18 years and older in Dakar Region and three other regions. Cervical samples were collected from 498 women aged 18-80 years (mean, 42.1 years) in Dakar Region. Also, 438 samples were collected from three other regions: Thiès, Saint-Louis, and Louga. The samples were screened for 21 HPV genotypes using an HPV type-specific E7 PCR bead-based multiplex genotyping assay (TS-MPG). The prevalence of high risk (HR)-HPV in Dakar Region was 17.4%. HPV 52 (3.2%) was the most prevalent HPV type, followed by HPV 31 (3.0%) and HPV 16, 45, and 53 (all 2.8%). In the Thiès, Saint-Louis, and Louga Regions, the prevalence of HR-HPV was 23.2%, 13.1%, and 19.4%, respectively. The study revealed the specificity of HPV prevalence in Dakar Region and other regions of Senegal. The observed patterns show some differences compared with other regions of the world. These findings raise the possibility that, in addition to HPV 16 and HPV 18, other HPV types should be considered for a vaccination program in Senegal. However, additional studies to determine the HPV type distribution in cervical cancer specimens in Senegal are required to further corroborate this hypothesis.
Topics: Adolescent; Adult; Aged; Aged, 80 and over; Female; Genotype; Genotyping Techniques; Humans; Middle Aged; Molecular Epidemiology; Papillomaviridae; Papillomavirus E7 Proteins; Papillomavirus Infections; Prevalence; Senegal; Young Adult
PubMed: 24026804
DOI: 10.1002/jmv.23719 -
Immunology Today Oct 1999
Topics: Autoimmunity; Humans; Lymphocyte Activation; Papillomaviridae; Psoriasis; T-Lymphocytes
PubMed: 10500318
DOI: 10.1016/s0167-5699(99)01517-0 -
Cancer May 2016
Topics: DNA, Viral; Human papillomavirus 16; Human papillomavirus 18; Humans; Papillomaviridae; Papillomavirus Infections
PubMed: 26990085
DOI: 10.1002/cncr.29979 -
Acta Dermato-venereologica Nov 2021
Topics: Alphapapillomavirus; Humans; Papillomaviridae; Warts
PubMed: 34806758
DOI: 10.2340/actadv.v101.567