-
Journal of Virology Nov 1989
Review
Topics: Animals; Female; Genes, Viral; Humans; Papilloma; Papillomaviridae; Tumor Virus Infections
PubMed: 2552162
DOI: 10.1128/JVI.63.11.4898-4903.1989 -
Current Topics in Microbiology and... 1994
Review
Topics: Female; Genes, Viral; Humans; Models, Biological; Papillomaviridae; Papillomavirus Infections; Tumor Virus Infections; Uterine Cervical Neoplasms
PubMed: 8205839
DOI: 10.1007/978-3-642-78487-3_8 -
Anales Del Sistema Sanitario de Navarra 2010Given the causal relationship between specific types of HPV with cervical cancer and precursor lesions, it is important to identify the viral type involved. The... (Review)
Review
Given the causal relationship between specific types of HPV with cervical cancer and precursor lesions, it is important to identify the viral type involved. The characterization is done by various methods that vary in sensitivity and specificity, whose use depends mainly on the aim pursued and the characteristics of the evaluated sample. HPV typing is not only important clinically for the establishment of monitoring and treatment of a patient, it also provides knowledge of the viral types circulating in a population, which is of interest in the development of prevention and treatment programs for this disease. This paper is a review of methods used in the detection and identification of HPV in cervical cancer lesions, highlighting their sensitivity and specificity.
Topics: DNA Probes, HPV; Humans; Papillomaviridae; Virology
PubMed: 20463773
DOI: 10.4321/s1137-66272010000100008 -
Journal of Clinical Microbiology Jul 2013Equine sarcoids are locally aggressive fibroblastic neoplasms considered to be the most common skin tumors of horses worldwide. Bovine papillomavirus types 1 and 2 have...
Equine sarcoids are locally aggressive fibroblastic neoplasms considered to be the most common skin tumors of horses worldwide. Bovine papillomavirus types 1 and 2 have typically been associated with sarcoids in equids. Investigations aiming to identify papillomavirus strains, aside from bovine papillomaviruses 1 and 2, which might be associated with sarcoid lesions, have been lacking. The aim of this article is to report the identification of a third bovine papillomavirus type, bovine papillomavirus 13, associated with equine sarcoids. Six sarcoid lesions were collected from diverse anatomical sites on two horses from southern Brazil. To detect a broad spectrum of papillomavirus strains, eight degenerate primer pairs designed to detect conserved regions on the L1 and E1 genes were tested on the DNA samples. Direct sequencing was then performed on the obtained amplicons, and sequence identities were compared with sequences from all bovine papillomavirus types. The FAP59/FAP64, MY09/MY11, and AR-E1F2/AR-E1R4 sequences generated from the sarcoids were shown to present 99 to 100% identity with bovine papillomavirus 13, a new bovine papillomavirus type previously described in cattle. The results from this study suggest that there is a need to identify bovine papillomavirus type 13 and other papillomavirus strains that might be associated with sarcoids in diverse geographical areas; such investigations might establish the frequency of occurrence of this viral type in these common tumors of equids.
Topics: Animals; Brazil; DNA, Viral; Horse Diseases; Horses; Molecular Sequence Data; Papillomaviridae; Sarcoidosis; Sequence Analysis, DNA
PubMed: 23637294
DOI: 10.1128/JCM.00371-13 -
Critical Reviews in Oncogenesis 1996Each human papillomavirus (HPV) type is genotypically distinct and infects epithelial cells at unique anatomic sites. Among the HPV types described, a subgroup is... (Review)
Review
Each human papillomavirus (HPV) type is genotypically distinct and infects epithelial cells at unique anatomic sites. Among the HPV types described, a subgroup is associated with genital disease and a subset of these is found in 90% of genital cancers. Although in benign infections the viral genome is present as an episome, in cancers it is integrated. The integration event invariably results in the expression of two viral proteins, E6 and E7. These two proteins are capable of transforming cells individually and cooperate to immortalize primary human epithelial cells. Molecular analysis has revealed that the E6 protein encoded by the HPV "high risk" types prevalent in cancers forms a tripartite complex with the p53 tumor suppressor protein and a cellular protein termed E6-AP, resulting in the degradation of p53. The E7 protein encoded by "high-risk" HPV types shows high-affinity association with the retinoblastoma tumor suppressor, pRb. The E7 protein associates also with other cellular factors known to play a role in cell cycle regulation. This review discusses the evidence, molecular and biological, in vitro and in vivo, supporting a direct role for the "high-risk" HPV type encoded E6 and E7 proteins in cervical carcinogenesis.
Topics: Amino Acid Sequence; Cell Cycle; Genome, Viral; Humans; Molecular Sequence Data; Neoplasms; Oncogene Proteins, Viral; Papillomaviridae
PubMed: 9109494
DOI: 10.1615/critrevoncog.v7.i1-2.10 -
Journal of Virology Mar 2020Human papillomavirus (HPV) type 58 is the third most commonly detected HPV type in cervical cancer among Eastern Asians. Our previous international epidemiological...
Human papillomavirus (HPV) type 58 is the third most commonly detected HPV type in cervical cancer among Eastern Asians. Our previous international epidemiological studies revealed that HPV58 carrying an E7 natural variant, T20I/G63S (designated V1), was associated with a higher risk of cervical cancer. We recently showed that V1 possesses a greater ability to immortalize and transform primary cells, as well as degrading pRB more effectively, than the prototype and other common variants. In this study, we performed a series of phenotypic and molecular assays using physiologically relevant and models to compare the oncogenicity of V1 with that of the prototype and other common natural variants. Through activation of the AKT and K-Ras/extracellular signal-regulated kinase (ERK) signaling pathways, V1 consistently showed greater oncogenicity than the prototype and other variants, as demonstrated by increased cell proliferation, migration, and invasion, as well as induction of larger tumors in athymic nude mice. This study complements our previous epidemiological and molecular observations pinpointing the higher oncogenicity of V1 than that of the prototype and all other common variants. Since V1 is more commonly found in eastern Asia, our report provides insight into the design of HPV screening assays and selection of components for HPV vaccines in this region. Epidemiological studies have revealed that a wild-type variant of HPV58 carrying an E7 variation, T20I/G63S (V1), is associated with a higher risk of cervical cancer. We previously reported that this increased oncogenicity could be the result of the virus's greater ability to degrade pRB, thereby leading to an increased ability to grow in an anchorage-independent manner. In addition to this, this report further showed that this HPV variant induced activation of the AKT and K-Ras/ERK signaling pathways, thereby explaining its genuine oncogenicity in promoting cell proliferation, migration, invasion, and formation of tumors, all to a greater extent than the prototype HPV58 and other common variants.
Topics: Animals; Asian People; Cell Proliferation; Disease Models, Animal; Female; Genetic Variation; Humans; Mice; Mice, Nude; Oncogene Proteins, Viral; Papillomaviridae; Papillomavirus Infections; Papillomavirus Vaccines; Rats; Uterine Cervical Neoplasms
PubMed: 31996427
DOI: 10.1128/JVI.00090-20 -
The Journal of Infectious Diseases Apr 2011
Topics: Cervix Uteri; Female; Humans; Incidence; Papillomaviridae; Papillomavirus Infections
PubMed: 21402540
DOI: 10.1093/infdis/jiq146 -
Annales de Biologie Clinique 1997Papillomaviruses are pathogens which induce cutaneous and mucosal lesions in man and in many animal species. The characterization of these viruses was rather low,... (Review)
Review
Papillomaviruses are pathogens which induce cutaneous and mucosal lesions in man and in many animal species. The characterization of these viruses was rather low, because viral infection cannot be fully reproduced in cell culture. The development of molecular biology techniques in the 1970s permitted to establish the remarkable plurality of the viruses, the tissue specificity and pecular pathogenicity linked to the type. Studies of the genome organization, the gene expression regulation and the protein characterization gave many informations leading to understand the mechanisms of viral-related carcinogenesis, especially the role of HPV16, the major risk factor for the development of squamous cervical carcinoma.
Topics: Genome, Viral; Humans; Papillomaviridae; Papillomavirus Infections; Transcription, Genetic; Tumor Virus Infections; Virus Replication
PubMed: 9499915
DOI: No ID Found -
The Tohoku Journal of Experimental... Aug 2020In Japan, a bivalent human papillomavirus (HPV) vaccine against carcinogenic HPV16/18 was licensed in 2009, and a quadrivalent vaccines against HPV16/18 and...
In Japan, a bivalent human papillomavirus (HPV) vaccine against carcinogenic HPV16/18 was licensed in 2009, and a quadrivalent vaccines against HPV16/18 and non-carcinogenic HPV6/11 was licensed in 2011. Recently, the next-generation 9-valent vaccine targeting HPV6/11/16/18/31/33/45/52/58 has been approved. Accurate HPV genotyping is essential for HPV vaccine research and surveillance. The Roche Linear Array (LA) has long been a standard assay for HPV genotyping, but its recent product discontinuation notice has urged us to introduce an alternative assay with comparable performance. In the present study, an in-house HPV genotyping assay that employs PCR with PGMY09/11 primers and reverse blotting hybridization (PGMY-CHUV) was compared with LA to assess genotype-specific agreement. A total of 100 cervical precancer specimens were subjected to both PGMY-CHUV and LA. For detection of genotypes included in the 9-valent vaccine, PGMY-CHUV completely agreed with LA for detection of HPV6, HPV11, HPV16, HPV18, HPV33 and HPV45, and showed near-complete agreement for HPV31 and HPV58 (98% and 99%, respectively). Moreover, PGMY-CHUV detected a significantly higher prevalence of HPV52 than LA (22% vs. 14%, P = 0.008 by McNemar's exact test), with 92.0% overall agreement, 63.6% positive agreement and a kappa value of 0.73. Most (87.5%) of HPV52 discordant cases involved mixed infections with HPV35 or HPV58. In conclusion, while the two assays present equivalent data for assessing the effectiveness of the bivalent and quadrivalent vaccines, PGMY-CHUV is more suitable for evaluating the impact of the current 9-valent vaccine because of its superior detection of HPV52 in co-infection cases.
Topics: Adult; Aged; Female; Genotype; Genotyping Techniques; Humans; Middle Aged; Papillomaviridae; Papillomavirus Vaccines; Young Adult
PubMed: 32759554
DOI: 10.1620/tjem.251.287 -
Journal of Clinical Virology : the... Jul 2009
Topics: Female; Humans; Molecular Diagnostic Techniques; Papillomaviridae; Papillomavirus Infections; Virology
PubMed: 19651362
DOI: 10.1016/S1386-6532(09)00331-X