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Proceedings, Annual Meeting of the... 1974
Topics: Humans; Papillomaviridae; Polyomaviridae
PubMed: 4534771
DOI: No ID Found -
Journal of Virology Jan 1975The DNA of the BK virus (BKV) human papovavirus was found to be heterogeneous, consisting of at least four discrete species of DNA. Only the largest of these four... (Comparative Study)
Comparative Study
The DNA of the BK virus (BKV) human papovavirus was found to be heterogeneous, consisting of at least four discrete species of DNA. Only the largest of these four species, BKV DNA (i), which has a molecular weight calculated to be 96% that of simian virus 40 (SV40) DNA, was infectious. Homogeneous preparations of BKV DNA were obtained, however, from virions purified after low multiplicity infections of human embryonic kidney cells. BKV DNA (i) was shown to contain a single R-Eco RI and four R-Hind cleavage sites. The R-Eco RI site was localized in the largest R-Hind cleavage fragment. Radiolabeled BKV DNA reassociated slightly faster than SV40 DNA; 20 to 30% polynucleotide sequence homology was demonstrated between the genomes of BKV and SV40 when the reaction was monitored by chromatography on hydroxyapatite.
Topics: Animals; Base Sequence; Cell Line; DNA, Viral; Endonucleases; Haplorhini; Humans; Kidney; Molecular Weight; Nucleic Acid Conformation; Phosphates; Phosphorus Radioisotopes; Polynucleotides; Polyomavirus; Simian virus 40
PubMed: 163342
DOI: 10.1128/JVI.15.1.173-181.1975 -
Cell Dec 1979The complete DNA sequence of human papovavirus BKV(Dun), consisting of 5153 nucleotide pairs, is presented. We describe the segments of the genome which correspond to...
The complete DNA sequence of human papovavirus BKV(Dun), consisting of 5153 nucleotide pairs, is presented. We describe the segments of the genome which correspond to the replication origin, the tandem repeated sequences, the 5' and 3' ends of the mRNAs, the splice sites, the early and late viral proteins and the putative viral polypeptides. These BKV DNA sequences are compared with analogous regions in the SV40 and Py virus genomes in an attempt to localize viral functions for lytic growth and transformation.
Topics: Amino Acid Sequence; Antigens, Viral; BK Virus; Base Sequence; DNA Replication; DNA, Viral; Genes, Viral; Humans; Polyomavirus; RNA, Messenger; RNA, Viral; Simian virus 40; Transcription, Genetic; Viral Proteins
PubMed: 229976
DOI: 10.1016/0092-8674(79)90209-5 -
The Journal of General Virology Feb 1981By electron microscopy of negatively stained urinary sediments, papovavirus particles of size intermediate between papillomavirus and polymavirus have been detected in...
By electron microscopy of negatively stained urinary sediments, papovavirus particles of size intermediate between papillomavirus and polymavirus have been detected in the urine of a pregnant woman. The excretion of size variants of these viruses and the absence of detectable papillomas in any of the women who were found to excrete only papillomavirus represent new findings.
Topics: Adult; Female; Humans; Microscopy, Electron; Papillomaviridae; Polyomaviridae; Polyomavirus; Pregnancy; Urine; Virion
PubMed: 6270238
DOI: 10.1099/0022-1317-52-2-359 -
Virology Oct 1986The hamster papovavirus (HaPV) is a polyoma virus with a restricted tumor spectrum. It is actively replicated in hair follicle tumors arising spontaneously in young... (Comparative Study)
Comparative Study
The hamster papovavirus (HaPV) is a polyoma virus with a restricted tumor spectrum. It is actively replicated in hair follicle tumors arising spontaneously in young Syrian hamsters. It can also induce lymphomas and leukemias in newborn hamsters. The complete nucleotide sequence of a cloned HaPV has been established recently. This report presents a comparison of this sequence with other polyomavirus genomes (polyoma, SV40, BKV, LPV) by matrix dot analysis and electron microscopy heteroduplex mapping. The results demonstrate a close relationship between the HaPV and the murine polyoma virus and designate the LPV as the closest relative among the primate polyomaviruses.
Topics: Animals; BK Virus; Biological Evolution; Cricetinae; DNA, Viral; Genes, Viral; Haplorhini; Mesocricetus; Microscopy, Electron; Nucleic Acid Heteroduplexes; Polyomavirus; Sequence Homology, Nucleic Acid; Simian virus 40; Species Specificity
PubMed: 3020783
DOI: 10.1016/0042-6822(86)90459-9 -
Journal of the National Cancer Institute Jul 1985Several epizootics of lymphoma occurred in a colony of LVG hamsters contaminated with an unusual, horizontally transmitted, subviral, lymphomagenic agent. Hamsters with...
Several epizootics of lymphoma occurred in a colony of LVG hamsters contaminated with an unusual, horizontally transmitted, subviral, lymphomagenic agent. Hamsters with horizontally transmitted lymphoma, or others housed with these hamsters, occasionally developed epitheliomas bearing an unclassified papovavirus. The possibility that the virus present in the wart-like structures in our hamster colony could activate lymphoma was tested, and a search was conducted for mature virions in passaged epitheliomas and lymphomas. The agent responsible for the skin epitheliomas in our hamster facility was an icosahedral, 36-nm virion compatible with the morphology of a polyomavirus or simian virus 40. Horizontally transmitted lymphoma cells and epitheliomas contained hamster papovavirus (HaPV) DNA sequences detected by dot hybridization; however, such sequences were not found in extracts of lymphomas with oncogenic potential. In contrast to reports by other investigators, infection of hamsters with the papovavirus present in primary epitheliomas produced epitheliomas in good yield but was not reproducibly associated with lymphoma induction. These data confirm the observation that the HaPV is the causative agent of epitheliomas, but they suggest clearly that HaPV is not the agent responsible for lymphomagenesis.
Topics: Animals; Carcinoma; Cricetinae; DNA, Neoplasm; DNA, Viral; Disease Outbreaks; Female; Lymphoma; Mesocricetus; Nucleic Acid Hybridization; Papillomaviridae; Polyomaviridae; Rodent Diseases; Skin Neoplasms
PubMed: 3859700
DOI: No ID Found -
Journal of Medical Virology 1981A novel member of the papovavirus group has been isolated from EBV-transformed African green monkey (AGM) B-lymphoblasts. The virus is characterized by its...
A novel member of the papovavirus group has been isolated from EBV-transformed African green monkey (AGM) B-lymphoblasts. The virus is characterized by its B-lymphotropic host range and has tentatively been named "lymphotropic papovavirus" (LPV). Seroepidemiological studies revealed that besides sera from African green monkeys a substantial proportion of human sera reacted with antigens of this virus. The specificity of this reaction observed in indirect immunofluorescence tests was underlined by immunoprecipitation (IP) and neutralization studies. By IP we demonstrated that AGM as well as human sera reacting in immunofluorescence with LPV antigens precipitated polypeptides of about 40 K. Those polypeptides were detected only in LPV-infected cultures. Sera of high reactivity in immunofluorescence tests also neutralized viral infectivity. These data suggest that an agent antigenetically closely related to AGM-LPV also infects part of the human population. The age distribution of the human antibody response revealed that a low percentage of sera reacted in age groups below 30 years. Thereafter, however, about 30% of all sera exhibited antibodies to LPV antigens. There was a slight increase in seroreactivity in sera from patients tested for infectious hepatitis when compared to non-selected human sera.
Topics: Age Factors; Animals; Antibodies, Viral; B-Lymphocytes; Cell Line; Chlorocebus aethiops; Fluorescent Antibody Technique; Humans; Lymphoma; Neutralization Tests; Papillomaviridae; Polyomaviridae; Virus Diseases
PubMed: 7017066
DOI: 10.1002/jmv.1890060405 -
The Journal of Infectious Diseases Feb 1980The effects of human leukocyte interferon on BK papovavirus (BKV) infection in 41 recipients of renal transplants were studied as part of a randomized, double-blind,... (Clinical Trial)
Clinical Trial Randomized Controlled Trial
The effects of human leukocyte interferon on BK papovavirus (BKV) infection in 41 recipients of renal transplants were studied as part of a randomized, double-blind, placebo-controlled trial. Eight transplant recipients demonstrated fourfold or greater rises in antibody to BKV, and three excreted BKV in urine. Neither seroconversion nor excretion was reduced by interferon administration. No clinical syndromes could be clearly linked to BKV infection. BKV was also relatively resistant to the in vitro effects of interferon. Pretreatment of interferon-sensitive human fibroblasts with up to 620 units of interferon/ml resulted in a loss of viral infectivity of one log or less. Continuous exposure of infected cultures to these interferon levels reduced BKV titers by 1.5-2.9 logs, whereas continuous exposure to lower concentrations of interferon had less effects. The levels shown to be marginally effective in vitro were considerably higher than those achieved in these patients' sera.
Topics: Animals; Antibodies, Viral; BK Virus; Humans; Interferons; Kidney Transplantation; Placebos; Polyomavirus; Tumor Virus Infections
PubMed: 6154110
DOI: 10.1093/infdis/141.2.157 -
Science (New York, N.Y.) Aug 1973Eighty-three percent of hamsters inoculated at birth with JC virus, a human papovavirus isolated from brain tissue of a case of progressive multifocal...
Eighty-three percent of hamsters inoculated at birth with JC virus, a human papovavirus isolated from brain tissue of a case of progressive multifocal leukoencephalopathy, developed malignant gliomas within 6 months. Three brain tumors have been serially transplanted as subcutaneous tumors. JC virus was isolated from five of seven tumors tested. Cells from four tumors were cultivated in vitro. These cells contained an intranuclear antigen with the characteristics of a T antigen, and this antigen was antigenically related to SV40 T antigen. Although virus was not recovered from extracts of serially cultured tumor cells, JC virus was rescued when one tumor cell line was fused with permissive cells.
Topics: Animals; Antibodies, Viral; Antigens, Viral; Brain Neoplasms; Cell Line; Cricetinae; Fluorescent Antibody Technique; Glioblastoma; Glioma; Humans; Leukoencephalopathy, Progressive Multifocal; Neoplasms, Experimental; Papillomaviridae; Polyomaviridae; Virus Cultivation
PubMed: 4353360
DOI: 10.1126/science.181.4100.674 -
Virology Jan 1970
Topics: Agar; Animals; Antigens; Cell Line; Cell Transformation, Neoplastic; Clone Cells; Complement Fixation Tests; Culture Techniques; Fluorescent Antibody Technique; Liver; Lung; Mice; Neoplasm Transplantation; Papillomaviridae; Polyomaviridae
PubMed: 4903857
DOI: 10.1016/0042-6822(70)90385-5