-
Ceskoslovenska Patologie Aug 1979Canine papillomatosis was diagnosed in 5 out of 12 dogs of the beagle race, aged three to four months, two weeks after arrival from a breeding station. The histological...
Canine papillomatosis was diagnosed in 5 out of 12 dogs of the beagle race, aged three to four months, two weeks after arrival from a breeding station. The histological as well as electronmicroscopical findings were typical of the disease.
Topics: Animals; Dog Diseases; Dogs; Female; Head and Neck Neoplasms; Male; Papillomaviridae; Polyomaviridae; Tumor Virus Infections
PubMed: 498342
DOI: No ID Found -
The Journal of Infectious Diseases Jun 1976Brain tissue from seven patients with progressive multifocal leukoencephalopathy was tested for the presence of papovaviruses. JC virus, ahuman papovavirus, was...
Brain tissue from seven patients with progressive multifocal leukoencephalopathy was tested for the presence of papovaviruses. JC virus, ahuman papovavirus, was identified in all seven cases. Virus was isolated in tissue culture from extracts from each of four patients and was detected by immunofluorescence in sections from the other three. The new osolates were indistinguishable from the prototypical JC strain serologically and in all biological characteristics examined. Thus JC virus has, to date, been associated with 20 cases of progressive multifocal leukoencephalopathy.
Topics: Adult; Antigens, Viral; Brain; Cells, Cultured; Cytopathogenic Effect, Viral; Female; Fluorescent Antibody Technique; Hemagglutination, Viral; Humans; Leukoencephalopathy, Progressive Multifocal; Male; Middle Aged; Papillomaviridae; Polyomaviridae; Serotyping; Virus Cultivation
PubMed: 778304
DOI: 10.1093/infdis/133.6.686 -
Journal of Comparative Pathology Oct 1994Newborn mice were inoculated orally with 100 LD50 of K-papovavirus and the distribution of virus in fatally infected animals was studied by in-situ nuclei acid...
Newborn mice were inoculated orally with 100 LD50 of K-papovavirus and the distribution of virus in fatally infected animals was studied by in-situ nuclei acid hybridization methods and immunoperoxidase staining for K virus capsid (V) antigen. Histopathologically, K virus produced extensive involvement of pulmonary endothelial cells, resulting in interstitial pneumonia, and widespread involvement of other endothelial cell populations throughout the systemic circulation. Endothelial cells in lungs, kidneys and other organs exhibited both specific hybridization for K virus nucleic acids and positive staining for K virus V antigen, indicative of productive infection. Scattered, apparently extravascular cells within brain parenchyma also exhibited both specific hybridization and immunohistological staining for K virus V antigen. In contrast, specific hybridization for K virus nuclei acids, in the absence of immunohistochemical labelling of K virus V antigen, suggesting transcription of viral DNA without expression of viral proteins, was detected in renal tubular epithelial cells and nonvascular, apparently lymphoid cells within the spleen and lymph nodes. The present study confirms the predominantly endotheliotropic nature of K virus infection in newborn mice and also demonstrates that the virus invades renal epithelial, lymphoid and possibly glial cells during primary infection.
Topics: Animals; Animals, Newborn; Antigens, Viral; In Situ Hybridization; Kidney Tubules; Liver; Lung; Lung Diseases, Interstitial; Mice; Papillomaviridae; Papillomavirus Infections; Polyomaviridae
PubMed: 7836567
DOI: 10.1016/s0021-9975(05)80004-0 -
Virology Apr 1989The human papovavirus BK latently infects a majority of the population worldwide, and its DNA has been found in human tumor tissue. BKV is known to be highly oncogenic...
The human papovavirus BK latently infects a majority of the population worldwide, and its DNA has been found in human tumor tissue. BKV is known to be highly oncogenic in rodents, and is capable of transforming cells in vitro. Rearrangements in the transcriptional regulatory sequences controlling expression of the transforming early gene, T antigen, are known to affect both the tumorigenic and transforming properties of this virus. Little is known about the mechanism by which this occurs. We have examined several aspects of BKV early promoter/enhancer regulation in cell types which the virus transforms, baby hamster kidney (BHK) and newborn rat kidney (NRK) cells, and compare them to the same processes in monkey kidney CV1 cells. We find that BKV early transcriptional efficiency requires the same enhancer repeat elements in all three cell types, but that requirements for sequences to the early and late side of these repeats vary between these cells. While the BKV T antigen was found to repress early gene expression from the BKV early promoter in CV1 cells, this effect was lower in BHK cells and essentially absent in NRK cells. The impaired autoregulation observed in rodent cells may be the result of inefficient T antigen production in these cells. DNA replication from the BKV origin was not detected in either BHK or NRK cells. Finally, we find no correlation between the efficiency of the BKV early regulatory region in directing gene expression and the ability to transform NRK cells.
Topics: Animals; Antigens, Viral, Tumor; BK Virus; Cell Transformation, Viral; Cells, Cultured; Cricetinae; DNA Mutational Analysis; Enhancer Elements, Genetic; Gene Expression Regulation; Genes, Viral; Polyomavirus; Promoter Regions, Genetic; Rats; Regulatory Sequences, Nucleic Acid; Transcription, Genetic; Virus Replication
PubMed: 2539697
DOI: 10.1016/0042-6822(89)90164-5 -
Journal of Virology Apr 1979JC human papovavirus was found to replicate in primary human amnion cells. The virus has undergone eight passages in amnion cells and was identified by serological...
JC human papovavirus was found to replicate in primary human amnion cells. The virus has undergone eight passages in amnion cells and was identified by serological methods as JC virus. By restriction endonuclease analysis of the viral DNA, the fragments observed were identical to those previously reported for the prototype strain.
Topics: Amnion; Antigens, Viral; Culture Techniques; Cytopathogenic Effect, Viral; DNA, Viral; Humans; Leukoencephalopathy, Progressive Multifocal; Molecular Weight; Papillomaviridae; Polyomaviridae; Virus Replication
PubMed: 480459
DOI: 10.1128/JVI.30.1.384-389.1979 -
Zentralblatt Fur Veterinarmedizin.... Jul 1993Tumorous, virus-induced skin lesions in two golden hamsters (Mesocricetus auratus) were characterized macroscopically and by means of light- and electron-microscopy....
Tumorous, virus-induced skin lesions in two golden hamsters (Mesocricetus auratus) were characterized macroscopically and by means of light- and electron-microscopy. Evidence of a virus was demonstrated in the ultra-thin sections and by the negative staining method. The morphological findings confirm the assumption that infections with papoviruses--probably of the polyomavirus genus--were involved.
Topics: Animals; Cricetinae; Female; Male; Mesocricetus; Microscopy, Electron; Polyomavirus Infections; Rodent Diseases; Skin; Tumor Virus Infections
PubMed: 8237205
DOI: 10.1111/j.1439-0450.1993.tb00147.x -
Transplantation Proceedings Mar 1981
Topics: Anemia, Aplastic; Animals; Antibodies, Viral; Antigens, Viral; Bone Marrow Transplantation; Graft Survival; Humans; Leukemia; Liver Diseases; Papillomaviridae; Polyomaviridae; Time Factors; Tumor Virus Infections; Urine
PubMed: 7022837
DOI: No ID Found -
Current Topics in Microbiology and... 1974
Review
Topics: Animals; Antigens, Viral; Cell Line; Cell Membrane; Cell Transformation, Neoplastic; Cricetinae; Cytopathogenic Effect, Viral; Genetic Code; Histocompatibility Antigens; Kidney; Models, Biological; Models, Theoretical; Neuraminidase; Papillomaviridae; Polyomaviridae; Simian virus 40
PubMed: 4364182
DOI: 10.1007/978-3-642-65775-7_5 -
Advances in Experimental Medicine and... 2006Polyomaviruses are small, tumorigenic, nonenveloped viruses that infect several different species. Interaction of these viruses with cell surface receptors represents... (Review)
Review
Polyomaviruses are small, tumorigenic, nonenveloped viruses that infect several different species. Interaction of these viruses with cell surface receptors represents the initial step during infection of host cells. This interaction can be a major determinant of viral host and tissue tropism. This chapter reviews what is currently known about the cellular receptors for each of five polyomavirus family members: Mouse polyomavirus (PyV), JC virus (JCV), BK virus (BKV), Lymphotropic papovavirus (LPV) and Simian virus 40 (SV40). These polyomaviruses serve to illustrate the enormous diversity of virus-cell surface interactions and allow us to closely evaluate the role of receptors in their life cycles. The contribution of other factors such as transcriptional regulators and signaling pathways are also summarized.
Topics: Polyomavirus; Receptors, Virus; Tropism
PubMed: 16626027
DOI: 10.1007/0-387-32957-9_4 -
Journal of Virology May 1989Transgenic mice have been generated which carry the early region of lymphotropic papovavirus (LPV). Eight of eleven founder animals died before 3 months of age after...
Transgenic mice have been generated which carry the early region of lymphotropic papovavirus (LPV). Eight of eleven founder animals died before 3 months of age after developing one or both of two distinct proliferative disorders. Of the three surviving animals, two are known to have rearranged or partial copies of the LPV genes. The majority of the founder animals (six) developed debilitating choroid plexus tumors by 26 to 42 days. Although this is the same tumor type induced by the simian virus 40 T-antigen gene, those induced by LPV appeared at a much younger age. The LPV early region was expressed in the brain tumors of these mice, as well as in the thymus and spleen. Expression in the latter two tissues reflects the cell-type specificity of the LPV enhancer demonstrated in cultured cells (i.e., lymphoid cells). Two founder animals (LP41 and LP50) gave rise to lines of mice that routinely develop lymphoproliferative disorders. LP50 and its LPV-positive offspring developed aggressive lymphomas and choroid plexus tumors. The transgenic offspring of LP41 also developed lymphomas. High levels of LPV RNA were expressed in the lymphomas of these mice as well as in the spleens and thymuses. The origin of the lymphomas from B- and T-cell lineages suggests that the LPV early genes are expressed in and can transform both of these cell types in vivo.
Topics: Animals; Blotting, Southern; Cell Transformation, Viral; Cerebral Ventricle Neoplasms; Choroid Plexus; Gene Expression Regulation; Genes, Viral; Lymph Nodes; Lymphoproliferative Disorders; Mice; Mice, Transgenic; Neoplasms, Experimental; Papillomaviridae; Pedigree; Polyomaviridae; RNA, Viral; Tissue Distribution
PubMed: 2704077
DOI: 10.1128/JVI.63.5.2204-2214.1989