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Haematologica Oct 2007
Topics: Aged; Antibodies, Monoclonal; Antibodies, Monoclonal, Murine-Derived; Antineoplastic Combined Chemotherapy Protocols; Cyclophosphamide; Dementia; Doxorubicin; Female; Humans; JC Virus; Leukoencephalopathy, Progressive Multifocal; Lymphoma, Mantle-Cell; Prednisolone; Rituximab; Vincristine
PubMed: 18024364
DOI: 10.3324/haematol.11259 -
Journal of Virological Methods Apr 1982Primary cultures of mouse embryo cells were inoculated with K virus, a murine papovavirus, and were examined for cytopathic effect (CPE) of for the development of...
Primary cultures of mouse embryo cells were inoculated with K virus, a murine papovavirus, and were examined for cytopathic effect (CPE) of for the development of fluorescent antibody staining specific for K virus V antigen. CPE was not observed. However, numerous cells in infected cultures exhibited positive nuclear fluorescence, and the presence of papovavirus virions was demonstrated by electron microscopy. Extracts from infected cultures produced typical K virus pneumonia in newborn mice. Inoculation of cultures with serial dilutions of virus demonstrated that these cells provide a fluorescent antibody assay for K virus equal in sensitivity to animal inoculation methods. Although specific K virus fluorescence was also detected in cultures of fetal mouse endocardial cells, livers, placentas, and brains, positive cells were much less abundant in these cultures than in cultures of mouse embryo cells. The mouse embryo culture assay described in the present paper represents the first method of measuring K virus infectivity in vitro.
Topics: Animals; Antigens, Viral; Cell Nucleus; Cells, Cultured; Cytopathogenic Effect, Viral; Embryo, Mammalian; Fluorescent Antibody Technique; Inclusion Bodies, Viral; Mice; Papillomaviridae; Polyomaviridae
PubMed: 7042725
DOI: 10.1016/0166-0934(82)90042-8 -
Journal of Virology Apr 1984Hamster embryo cells were transformed by African green monkey lymphotropic papovavirus (LPV). The transformed cells contained intranuclear T-antigens demonstrable by...
Hamster embryo cells were transformed by African green monkey lymphotropic papovavirus (LPV). The transformed cells contained intranuclear T-antigens demonstrable by fluorescent antibody staining with hamster anti-LPV serum. Analysis of uncloned and cloned lines of transformed cells for LPV sequences revealed that the viral DNA was present as free nonintegrated and integrated genomes; there were approximately 10 copies of free DNA and about one to two copies of integrated genomes per cell. The cells were highly tumorigenic when inoculated into hamsters and produced progressively growing tumors in 100% of newborn or 10-day-old hamsters that were inoculated with LPV-transformed cells. The serum from tumor-bearing hamsters reacted with LPV-transformed cells and also showed a weak reaction with simian virus 40-, BK virus-, and JC virus-transformed cells, thereby showing an antigenic relationship with the T-antigens of other primate polyomaviruses. The large T-antigen of LPV was found to be an 84,000-molecular-weight protein which was immunoprecipitated by hamster anti-LPV (antiviral) as well as by tumor serum.
Topics: Agar; Animals; Antigens, Viral, Tumor; Blood; Cell Division; Cell Line; Cell Transformation, Neoplastic; Cell Transformation, Viral; Clone Cells; Cricetinae; Culture Media; DNA, Viral; Embryo, Mammalian; Neoplasms, Experimental; Polyomavirus; Tumor Virus Infections
PubMed: 6321782
DOI: 10.1128/JVI.50.1.100-105.1984 -
International Journal of Cancer Nov 1979A human glioblastoma multiforme (M27) tested in early cell cultures by indirect immunofluorescence staining showed SV40-related tumor (T)-antigen, 95% of the cells being...
A human glioblastoma multiforme (M27) tested in early cell cultures by indirect immunofluorescence staining showed SV40-related tumor (T)-antigen, 95% of the cells being positive. SV40-related viral capsid (V)-antigen was absent in all cells tested. Experiments to rescue this virus were performed by fusing M27 cells with CV-I monkey cells, which were permissive for SV40, using polyethylene glycol (PEG) as fusion factor. We succeeded in isolating virus particles SV40-GBM which electron microscopy showed to correspond in size and morphology to papovaviruses. Serological tests (hemagglutination, neutralization, fluorescent antibody) revealed that the virus is indistinguishable from SV40. Despite this apparent antigenic identity SV40-GBM differs slightly from SV40 wild type. This virus can propagate and produce CPE in both CV-I cells and primary fetal human kidney cells. Furthermore digestion of SV40-GBM DNA with the HindII/III restriction endonucleases revealed minor differences compared with the SV40 DNA. Therefore the virus SV40-GBM obtained from glioblastoma cells seems to be closely related to the SV40-PML viruses described earlier.
Topics: Antigens, Viral; Autoradiography; Cell Fusion; Cell Nucleus; Cells, Cultured; DNA, Viral; Electrophoresis, Polyacrylamide Gel; Epitopes; Female; Fluorescent Antibody Technique; Glioblastoma; Hemagglutination Tests; Humans; Karyotyping; Microscopy, Electron; Middle Aged; Neutralization Tests; Papillomaviridae; Polyomaviridae
PubMed: 93581
DOI: 10.1002/ijc.2910240502 -
Proceedings of the European Dialysis... 1980Kidney biopsies from 98 patients were studied for BK virus (BKV) antigens by indirect immunofluorescence. Intense fluorescent staining was observed in 11/12 cases of...
Kidney biopsies from 98 patients were studied for BK virus (BKV) antigens by indirect immunofluorescence. Intense fluorescent staining was observed in 11/12 cases of lupus nephritis, 11/12 cases of membranous nephropathy, 21/23 cases of IgA mesangial glomerulonephritis, 3/4 cases of membrano-proliferative Glomerulonephritis and 5/12 cases of exudative glomerulonephritis. Antisera of different viruses did not react with any kidney sample. Deposition of BKV was strictly related to the presence of immunoglobulins in renal glomeruli. The specificity of reaction for antigens related to BKV was demonstrated by absorption of sera with different substances and BKV. Absorption of rabbit anti-BKV serum with human immunoglobulins completely abolished glomerular fluorescence. We conclude that the fluorescence obtained in kidney biopsies by staining with anti-BKV serum was a false positive reaction dependent on common antigenic determinants present in BKV capsid proteins and human immunoglobulins.
Topics: Antigen-Antibody Complex; Antigens, Viral; BK Virus; Cross Reactions; False Positive Reactions; Fluorescent Antibody Technique; Glomerulonephritis; Humans; Kidney; Polyomavirus
PubMed: 6264423
DOI: No ID Found -
Infection and Immunity Jul 1976A new viral agent, stumptailed macaque virus (STMV), isolated from uninoculated stumptailed macaque kidney cultures was identified. The virions had the size and...
A new viral agent, stumptailed macaque virus (STMV), isolated from uninoculated stumptailed macaque kidney cultures was identified. The virions had the size and morphology of papovaviruses of the simian virus 40 (SV40)-polyoma subgroup, but many of them appeared to have an additional outer envelope. The deoxyribonucleic acid of STMV was a superhelical circular molecule, with a mean length 91% of that of SV40. The antigenic relationship of this virus with other members of the group was examined by immune electron microscopy of isolated virions and by immunofluorescent staining of virus-infected cells. STMV was immunologically distinct from SV40, BK virus (BKV), polyoma virus, and JC virus. Its tumor antigen may be related to those of SV40 and BKV.
Topics: Animals; Antigens, Viral; DNA, Circular; DNA, Viral; Epitopes; Haplorhini; Kidney; Macaca; Molecular Weight; Papillomaviridae; Polyomaviridae; Polyomavirus; Simian virus 40
PubMed: 59705
DOI: 10.1128/iai.14.1.225-231.1976 -
Cell Jul 1986Transgenic mice containing the early region of human papovavirus JC were produced. Some of these mice exhibited a shaking disorder similar to the previously described...
Transgenic mice containing the early region of human papovavirus JC were produced. Some of these mice exhibited a shaking disorder similar to the previously described mutant mice jimpy or quaking. Neuropathological analysis indicated a dysmyelination in the central nervous system, but not the peripheral nervous system. A high level of JCV T-antigen mRNA was present in the brains of the mice exhibiting the myelin disorder. JC virus is associated in humans with a degenerative demyelinating disease: progressive multifocal leukoencephalopathy. The JCV-containing transgenic mice may therefore provide an animal model for studying this disease.
Topics: Animals; Antigens, Viral, Tumor; Demyelinating Diseases; Genes, Viral; JC Virus; Mice; Neoplasms, Experimental; Pedigree; Polyomavirus; RNA, Messenger; RNA, Viral; Transfection
PubMed: 3013417
DOI: 10.1016/0092-8674(86)90855-x -
The Journal of General Virology Oct 1976A comparison was made of the T antigens induced in transformed cells or infected permissive cells by representatives of three categories of human papovavirus. The... (Comparative Study)
Comparative Study
A comparison was made of the T antigens induced in transformed cells or infected permissive cells by representatives of three categories of human papovavirus. The transformed hamster cell lines employed contained T antigen induced by either the BK or RF strains of papovavirus associated with human renal allografts; the JC strain of papovavirus from progressive multifocal leukoencephalopathy (PML), or a variant of SV40 virus isolated from PML. The human papovavirus T antigens were also compared with that of a human cell line transformed by SV40 of simian origin. Anti-T antibody prepared in hamsters against each of the hamster cell lines was absorbed with crude T antigen from each cell line, and the unabsorbed and absorbed antisera were tested for residual T antibody against each cell line, or against infected permissive cells by immunoperoxidase (IP) staining and complement-fixation (CF) tests. In unabsorbed antisera, T antibodies from each cell line cross-reacted with all T antigens in IP tests, and CF tests showed that T antisera reacted preferentially with T antigen induced by homologous virus. Absorpminants. T antigens of the two urine-derived strains, BK and RF, were identical or nearly so, but were clearly separable from T antigens of JC virus, PML-derived SV40 or simian-derived SV40. JC T antigen was intermediate, being more closely related to T antigens both of BK virus and SV40 virus than the latter were to each other. The T antigen of PML-derived SV40 could be distinguished from the T antigen of simian-derived SV40 and the T antigen of the SV40 variant from human brain was more closely related to those of the other human-derived papovaviruses than was the T antigen of SV40 from monkey kidney.
Topics: Animals; Antigens, Viral; BK Virus; Cell Line; Cell Transformation, Neoplastic; Complement Fixation Tests; Cricetinae; Cross Reactions; Epitopes; Histocompatibility Antigens; Humans; Immunoenzyme Techniques; Papillomaviridae; Polyomaviridae; Simian virus 40
PubMed: 62024
DOI: 10.1099/0022-1317-33-1-61 -
Journal of Nephrology 1999Increasing attention has been recently accorded to BK and JC viruses (BKV and JCV). Both these human polyomavirus (HPV) are members of the papovavirus family which... (Review)
Review
Increasing attention has been recently accorded to BK and JC viruses (BKV and JCV). Both these human polyomavirus (HPV) are members of the papovavirus family which includes the simian virus SV 40. BKV and JCV infect more than 60% of the population worldwide. After primary infection, they remain harboured in the kidneys and may become reactivated in situations of immune impairment. HPV were first described in 1971. BKV was isolated in a renal transplant patient with ureteral stricture and JCV in a patient with progressive multifocal leukoencephalopathy (PML). BKV was known to be involved in post-bone marrow transplantation (BMT) hemorrhagic cystitis. In renal transplantation, BKV and JCV were initially found in the post-transplant ureteric stricture and PML. They are now recognised as a possible cause of transplant interstitial nephritis, mimicking rejection (satisfying the Banff criteria for acute rejection) or drug toxicity. In HPV nephritis there is a mixed interstitial inflammatory infiltrate with focal tubular injury; the tubular epithelium shows marked anisonucleosis, nuclear atypia and basophilic or amphophilic intranuclear inclusions. Tubulitis is frequent. DNA hybridisation or gene amplification by polymerase chain reaction usually demonstrate HPV. Although histology with viral nucleic acid detection may be helpful in differentiating viral infection and rejection, confusion between these complications may lead to either anti-rejection therapy, with the risk of over-immunosuppression, or reduction of immunosuppression, with the risk of graft loss. Confusion may also arise with inclusions of other viruses, such as cytomegalovirus, herpes virus and adenovirus. Reactivation of BKV and JCV infection was demonstrated in respectively 22.2% and 10.9% of renal transplant recipients and 55% and 6.7% of BMT patients. Unfortunately, no routine screening is available for these viruses, so this complication is probably underestimated. No specific therapy of HPV infection is currently available.
Topics: Bone Marrow Transplantation; Humans; Kidney Transplantation; Polyomavirus; Polyomavirus Infections; Postoperative Complications; Tumor Virus Infections
PubMed: 10202999
DOI: No ID Found