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Journal of Virology Jul 1977The DNA of three cloned lines of hamster kidney cells transformed by human papovavirus BK DNA was examined by reassociation kinetics for viral sequences and found to...
The DNA of three cloned lines of hamster kidney cells transformed by human papovavirus BK DNA was examined by reassociation kinetics for viral sequences and found to contain 2.7 to 5.3 equivalents of viral DNA per diploid genome. In the one line examined with the four R-HindIII fragments of the human papovavirus BK genome, the entire viral genome was uniformly represented.
Topics: Animals; BK Virus; Base Sequence; Cell Line; Cell Transformation, Neoplastic; Chromosome Mapping; Clone Cells; Cricetinae; DNA Restriction Enzymes; DNA, Neoplasm; DNA, Viral; Polyomavirus
PubMed: 196104
DOI: 10.1128/JVI.23.1.205-208.1977 -
Journal of Virology Mar 1976Complementation tests between BK human papovavirus and SV40 temperature-sensitive mutants tsA58 and tsB11 were performed. Under the reported experimental conditions, BKV...
Complementation tests between BK human papovavirus and SV40 temperature-sensitive mutants tsA58 and tsB11 were performed. Under the reported experimental conditions, BKV complemented the "early" mutant tsA58 but failed to complement the "late" mutant tsB11.
Topics: Antigens, Viral; BK Virus; DNA Replication; DNA, Viral; Genetic Complementation Test; Humans; Mutation; Polyomavirus; Recombination, Genetic; Simian virus 40; Temperature; Urine; Virus Replication; Wiskott-Aldrich Syndrome
PubMed: 176441
DOI: 10.1128/JVI.17.3.1060-1062.1976 -
Journal of Virology May 1982The growth of African green monkey lymphotropic papovavirus (LPV) in human lymphoblastoid cell line BJA-B was found to be slow and inefficient due to the accumulation of...
The growth of African green monkey lymphotropic papovavirus (LPV) in human lymphoblastoid cell line BJA-B was found to be slow and inefficient due to the accumulation of defective particles. An analysis of molecularly cloned LPV DNAs showed that 3 of 19 clones had DNAs that were longer (5.1 kilobases) than the DNAs of the other clones. The 5.1-kilobase DNA was infectious for BJA-B cells, whereas the shorter (4.8-kilobase) molecules were defective. Unlike the wild-type virus, stocks of LPV made from cloned, infectious DNAs were homogeneous and had higher titers. Using stocks of nondefective LPV, we investigated other biological properties. LPV replication in another human B-lymphoblastoid cell line was observed. The virus did not cause tumors when it was inoculated into newborn hamsters. Serological surveys of human and nonhuman primate sera indicated that virtually all primates, including humans, show evidence of infection by viruses antigenically related to LPV.
Topics: Animals; Chlorocebus aethiops; Cloning, Molecular; Cricetinae; DNA, Viral; Hemagglutination, Viral; Lymphocytes; Papillomaviridae; Polyomaviridae; Virulence
PubMed: 7086969
DOI: 10.1128/JVI.42.2.502-509.1982 -
Proceedings of the National Academy of... Jun 1987Human papovavirus JCV is associated with the human demyelinating disorder progressive multifocal leukoencephalopathy. In tissue culture, the virus is largely restricted...
Human papovavirus JCV is associated with the human demyelinating disorder progressive multifocal leukoencephalopathy. In tissue culture, the virus is largely restricted to growth in primary human fetal glial cell. In this study, we demonstrate two levels of regulation of the viral host range. Expression of the early JCV mRNA, which encodes the essential viral protein, large tumor antigen (T antigen), depends on recognition of the early enhancer/promoter elements by tissue-specific factors found in both human and rodent glial cells. In the presence of JCV T antigen, viral DNA replication requires a species-specific factor, presumably a component of DNA polymerase, which is found in a wide range of primate cells. We further demonstrate that simian virus 40 T antigen has sufficient homology to efficiently substitute for the analogous JCV protein in initiating viral DNA replication.
Topics: Animals; Cell Line; Cell Transformation, Viral; Cells, Cultured; DNA Replication; Fetus; Humans; JC Virus; Plasmids; Polyomavirus; Transfection; Virus Replication
PubMed: 3035549
DOI: 10.1073/pnas.84.11.3695 -
Avian Pathology : Journal of the W.V.P.A 1987Outbreaks of a disease in two budgerigar aviaries caused 20% and 53% mortality respectively in recently hatched chicks during the 1985 breeding season. Clinical signs...
Outbreaks of a disease in two budgerigar aviaries caused 20% and 53% mortality respectively in recently hatched chicks during the 1985 breeding season. Clinical signs were first observed at 5 to 7 days of age. In most birds darkening of the normal skin colour was followed by death in 2 to 10 days. In individual nests all or part of a hatch could be affected. Occasional survivors had abnormal plumage. Few gross lesions were found at post-mortem examination. Histologically, many large basophilic intranuclear inclusions were present in multifocal degenerative lesions throughout the epidermis, kidney, heart, brain and other tissues. These inclusions were shown by transmission electron microscopy to contain numerous virus particles which, on direct examination, had a .diameter of 46 to 56 nm and a papovavirus-like morphology. These outbreaks were considered to be cases of budgerigar fledgling disease, a syndrome that has been reported in other countries but not previously described in Great Britain.
PubMed: 18766650
DOI: 10.1080/03079458708436411 -
Italian Journal of Neurological Sciences Dec 1996Progressive multifocal leucoencephalopathy (PML) is a rarely occurring demyelinating disease of the central nervous system caused by a neurotropic papovavirus named JC... (Review)
Review
Progressive multifocal leucoencephalopathy (PML) is a rarely occurring demyelinating disease of the central nervous system caused by a neurotropic papovavirus named JC virus (JCV). The most frequently affected affected regions are the cerebral hemispheres, especially the parietooccipital region, followed by the cerebellum and brain stem. The disease occurs predominantly in individuals with an immunocompromised state and impaired cellular mediated immunity (CMI) due to other underlying illness. More extensive use of irradiation and immunosuppressive therapy in relation to increased transplantational activities as well as treatment of autoimmune diseases and malignancies, in addition to the appearance of the acquired immunodeficiency syndrome (AIDS) as a consequence of infection with the human immunodeficiency virus (HIV), has caused a considerable increase in the occurrence of PML. The course of the disease is still most often rapidly progressive and fatal, but several cases with prolonged survival and even remission have been reported, and various antiviral treatments have been tried. The only drug that until now has shown favourable results is cytosine arabinoside. In HIV-infected PML-patients immunomodulation with AZT/zidovudine may alleviate the course and improve the prognosis in some patients. Suspicion of PML should lead to an extensive immunological investigation before considering of brain biopsy, which is still the only specific test. On the basis of the increased frequency of PML in relation to HIV-infection, it is likely that our knowledge of the pathogenetic aspects will increase, which, hopefully, may lead to an effective therapeutic strategy.
Topics: Adult; Child; DNA, Viral; Humans; JC Virus; Leukoencephalopathy, Progressive Multifocal
PubMed: 8978445
DOI: 10.1007/BF01997713 -
Journal of Virology Jan 1975Supercoiled BK papovavirus DNA was shown to transform hamster kidney cells using the calcium phosphate co-precipitation technique. The transformed cells contained...
Supercoiled BK papovavirus DNA was shown to transform hamster kidney cells using the calcium phosphate co-precipitation technique. The transformed cells contained intranuclear T-antigen(s) and rescuable virus and produced progressively growing tumors when inoculated into hamsters. A novel finding was the production in tumor-bearing animals of antinuclear antibody, which reacted against normal, untransformed cells; in addition, tumor serum contained antibody against virus-specific T-antigen(s).
Topics: Animals; Antibodies, Antinuclear; Antibodies, Neoplasm; Antigens, Neoplasm; Antigens, Viral; BK Virus; Calcium Phosphates; Cell Fusion; Cell Line; Cell Nucleus; Cell Transformation, Neoplastic; Chemical Precipitation; Cricetinae; DNA, Viral; Kidney; Neoplasms, Experimental; Polyomavirus; Virus Replication
PubMed: 173887
DOI: 10.1128/JVI.17.1.247-253.1976 -
Journal of Virology May 2000Small DNA tumor viruses like human papillomaviruses, simian virus 40, and adenoviruses modulate the activity of cellular tumor suppressor proteins p53 and/or pRB. These...
Small DNA tumor viruses like human papillomaviruses, simian virus 40, and adenoviruses modulate the activity of cellular tumor suppressor proteins p53 and/or pRB. These viruses replicate as nuclear multicopy extrachromosomal elements during the S phase of the cell cycle, and it has been suggested that inactivation of p53 and pRb is necessary for directing the cells to the S phase. Mouse polyomavirus (Py), however, modulates only the pRB protein activity without any obvious interference with the action of p53. We show here that Py replication was not suppressed by the p53 protein indeed in all tested different mouse cell lines. In addition, E1- and E2-dependent papillomavirus origin replication was insensitive to the action of p53 in mouse cells. We show that in hamster (Chinese hamster ovary) or human (osteosarcoma 143) cell lines the replication of both Py and papillomavirus origins was efficiently blocked by p53. The block of Py replication in human and hamster cells is not caused by the downregulation of large T-antigen expression. The deletion analysis of the p53 protein shows that the RPA binding, proline-rich regulatory, DNA-binding, and oligomerization domains are necessary for p53 action in both replication systems. These results indicate that in mouse cells the p53 protein could be inactive for the suppression of papovavirus replication.
Topics: Animals; Bovine papillomavirus 1; CHO Cells; Cell Line; Cricetinae; Fibroblasts; Gene Expression Regulation, Viral; Humans; Immunoblotting; Mice; Plasmids; Polyomavirus; Polyomavirus Infections; Replication Origin; Transfection; Tumor Cells, Cultured; Tumor Suppressor Protein p53; Tumor Virus Infections; Virus Replication
PubMed: 10775606
DOI: 10.1128/jvi.74.10.4688-4697.2000 -
Nature Mar 1980
Topics: Antigens, Neoplasm; Antigens, Viral; BK Virus; Base Sequence; DNA, Viral; Genes; Humans; Polyomavirus; Species Specificity
PubMed: 6244496
DOI: 10.1038/284124a0 -
British Journal of Hospital MedicineProgressive multifocal leucoencephalopathy (PML) is a progressive, usually fatal, demyelinating disease of the CNS that is associated with infection of oligodendrocytes... (Review)
Review
Progressive multifocal leucoencephalopathy (PML) is a progressive, usually fatal, demyelinating disease of the CNS that is associated with infection of oligodendrocytes by the papovavirus JC. The incidence of PML is rising as a consequence of the AIDS epidemic.
Topics: Brain; Humans; JC Virus; Leukoencephalopathy, Progressive Multifocal; Simian virus 40
PubMed: 8401896
DOI: No ID Found