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Ocular Immunology and Inflammation May 2022
Topics: Angiogenesis Inhibitors; Anti-Bacterial Agents; Bacteria; Endophthalmitis; Eye Infections, Bacterial; Humans; Intravitreal Injections; Paracoccus; Retrospective Studies
PubMed: 33216649
DOI: 10.1080/09273948.2020.1836233 -
IDCases 2019is a Gram-negative coccobacilli which is often an environmental organism. However, infection of patients usually with underlying immunosuppression has been described in...
INTRODUCTION
is a Gram-negative coccobacilli which is often an environmental organism. However, infection of patients usually with underlying immunosuppression has been described in the last decades, mainly due to the emergence of diagnostic molecular methods.
CASE PRESENTATION
We describe here a case of peritonitis in a patient undergoing peritoneal dialysis. Turbidity of the peritoneal dialysate was the sole clinical manifestation. Inflammatory markers were not raised. A peritoneal fluid specimen showed increased white-cell count, but no organisms were seen on Gram stain. identified as the infectious agent. Patient was successfully treated with gentamicin. Minimum inhibitory concentration analysis suggested to be sensitive to aminoglycosides and specific betalactams but not to ciprofloxacin and ceftazidime, in line with previous literature.
DISCUSSION
This case of peritoneal-dialysis peritonitis contributes to accumulating evidence on the emergent role of this organism as a relevant human pathogen. It also provides information about antibiotic resistance patterns that helps to guide therapy more specifically and effectively.
PubMed: 30701158
DOI: 10.1016/j.idcr.2019.e00486 -
Clinical Laboratory Aug 2019Parotid cyst is a common problem in patients treated by surgeons. However, Paracoccus yeei was isolated from an aerobic blood culture in a patient with parotid cyst as...
BACKGROUND
Parotid cyst is a common problem in patients treated by surgeons. However, Paracoccus yeei was isolated from an aerobic blood culture in a patient with parotid cyst as an unusual etiologic opportunistic agent.
METHODS
Since old biochemical identification kits are not able to identify this species, MALDI-TOF MS correctly was recommended to identify this isolate. Its identity was confirmed by sequencing of the 16S rRNA gene.
RESULTS
The aligned sequences (16S rRNA gene) were used for a phylogenetic analysis (phylogenetic tree), which was produced using the BLAST pair-wise alignments. The sequence analysis determined that the best matches were with Paracoccus yeei.
CONCLUSIONS
Paracoccus yeei has been reported as a rare opportunistic human pathogen, we should actively com-municate to the clinic to improve the real positive rate.
Topics: Bacterial Typing Techniques; Cysts; DNA, Bacterial; Humans; Male; Middle Aged; Paracoccus; Parotid Diseases; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 31414739
DOI: 10.7754/Clin.Lab.2019.190125 -
Nefrologia : Publicacion Oficial de La... 2016
Topics: Animals; Catheter-Related Infections; Dogs; Environmental Exposure; Female; Gram-Negative Bacterial Infections; Humans; Middle Aged; Paracoccus; Peritoneal Dialysis; Peritonitis; Soil Microbiology
PubMed: 27039709
DOI: 10.1016/j.nefro.2016.02.009 -
Eye & Contact Lens May 2014Paracoccus yeei is an environmental bacterium suspected to be responsible for ocular infections. In this study, culture-based and polymerase chain reaction (PCR)...
OBJECTIVES
Paracoccus yeei is an environmental bacterium suspected to be responsible for ocular infections. In this study, culture-based and polymerase chain reaction (PCR) sequencing-based investigations of a corneal scraping specimen confirmed P. yeei keratitis in one contact lens wearer.
METHODS
A 34-year-old male patient, who was a contact lens wearer, presented with a unilateral corneal ulcer. A corneal scraping was performed to conduct a microbiological investigation. Disease progress was favorable after a 5-week topical treatment, which combined rifampicin, ciprofloxacine, tobramycine, and dexamethasone. The final visual acuity of the right eye was 5/10 with correction, limited by a central corneal scar.
RESULTS
Culturing the corneal scraping specimen yielded P. yeei, which was also directly detected by PCR sequencing the bacterial 16S rDNA in the presence of negative controls. The P. yeei isolate was susceptible to ticarcillin, with a minimal inhibitory concentration (MIC) of <4 mg/L of ceftazidime (MIC of 0.5 mg/L), imipenem (MIC < 0.5 mg/L), gentamycin (MIC of 0.5 mg/L), tobramycin (MIC < 0.06 mg/L), rifampicin (MIC < 0.125 mg/L), ciprofloxacin (MIC < 0.5 mg/L), and colimycin (MIC < 2 mg/L). The isolate was resistant to fosfomycine with a MIC 32 > mg/L.
CONCLUSIONS
P. yeei is a new cause of keratitis that must be diagnosed by a laboratory analysis of corneal scrapings due to the potentially poor outcome of infection.
Topics: Adult; Anti-Bacterial Agents; Contact Lenses; Corneal Ulcer; Drug Therapy, Combination; Humans; Male; Paracoccus; Treatment Outcome
PubMed: 24045834
DOI: 10.1097/ICL.0b013e31829e8fc7 -
3 Biotech May 2020This work investigated the properties of VKM B-3302 bacteria isolated from activated sludge and immobilized in an N-vinylpyrrolidone-modified poly(vinyl alcohol)...
This work investigated the properties of VKM B-3302 bacteria isolated from activated sludge and immobilized in an N-vinylpyrrolidone-modified poly(vinyl alcohol) matrix. The developed hydrogel formed a network structure to enable the entrapment of microbial cells with their viability and biocatalytic properties preserved, which ensured the technological possibility of replicating expendable biosensor receptor elements. A new ratio of the components for the synthesis selected in this work enabled producing a copolymer of an earlier undescribed chemical structure, which can be efficiently used for immobilization of highly sensitive bacteria. A biological oxygen demand (BOD) biosensor with these bacteria and matrix was shown to possess a long-time stability exceeding that described earlier, to have a broad substrate specificity and to exceed approximately tenfold the nearest analogues by its sensitivity and the lower boundary value of 0.05 mg/dm. The biosensor enabled assays of water samples initially attributed to pure samples (the BOD range, 0.05-5.0 mg/dm). BOD assays of water samples from various sources showed the use of the receptor element of this composition to enable the data that closely correlated with the standard method ( = 0.9990).
PubMed: 32346498
DOI: 10.1007/s13205-020-02199-0 -
Therapeutic Apheresis and Dialysis :... Oct 2021
Topics: Anti-Bacterial Agents; Ceftriaxone; Gram-Negative Bacterial Infections; Humans; Male; Middle Aged; Paracoccus; Peritoneal Dialysis; Peritonitis
PubMed: 33135289
DOI: 10.1111/1744-9987.13604 -
Clinical Case Reports May 2021, a Gram-negative coccobacillus, is an emergent opportunistic pathogen. It originates from soil and water. VITEK and MALDI-TOF are used for identification. There are few...
, a Gram-negative coccobacillus, is an emergent opportunistic pathogen. It originates from soil and water. VITEK and MALDI-TOF are used for identification. There are few reports of peritoneal dialysis peritonitis. Its presentation is usually indolent. It can be successfully treated with several antibiotics: β-lactams, aminoglycosides, without removing the catheter.
PubMed: 34026181
DOI: 10.1002/ccr3.4176 -
Genome Announcements Jan 2018TT13 was isolated from human skin because of its ability to degrade propylene glycol. Here, we present the whole-genome sequence of this strain; it possesses one...
TT13 was isolated from human skin because of its ability to degrade propylene glycol. Here, we present the whole-genome sequence of this strain; it possesses one 3.58-Mb chromosome and six plasmids. TT13 genome analysis indicated that this bacterium has denitrification potential.
PubMed: 29348361
DOI: 10.1128/genomeA.01514-17 -
Frontiers in Microbiology 2018Bacteria of the genus are common components of the microbiomes of many naturally- and anthropogenically shaped environments. One species, , is unique within the genus...
Bacteria of the genus are common components of the microbiomes of many naturally- and anthropogenically shaped environments. One species, , is unique within the genus because it is associated with opportunistic human infections. Therefore, strains of may serve as an interesting model to study the transition from a saprophytic to a pathogenic lifestyle in environmental bacteria. Unfortunately, knowledge concerning the biology, genetics and genomic content of is fragmentary; also the mechanisms of pathogenicity of this bacterium remain unclear. In this study we provide the first insight into the genome composition and metabolic potential of a clinical isolate, CCUG 32053. This strain has a multipartite genome (4,632,079 bp) composed of a circular chromosome plus eight extrachromosomal replicons pYEE1-8: 3 chromids and 5 plasmids, with a total size of 1,247,173 bp. The genome has been significantly shaped by the acquisition of genomic islands, prophages ( and phage families) and numerous insertion sequences (ISs) representing seven IS families. Detailed comparative analysis with other complete genomic sequences of spp. (including FDAARGOS_252 and TT13, as well as non-pathogenic strains of other species in this genus) enabled us to identify species-specific genes and to predict putative determinants of virulence. This is the first attempt to identify pathoadaptive genetic information of and to estimate the role of the mobilome in the evolution of pathogenicity in this species.
PubMed: 30410477
DOI: 10.3389/fmicb.2018.02553