Did you mean: peptoniphilus haeri
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Gastroenterology Dec 2023Pien Tze Huang (PZH) is a well-established traditional medicine with beneficial effects against inflammation and cancer. We aimed to explore the chemopreventive effect...
BACKGROUND & AIMS
Pien Tze Huang (PZH) is a well-established traditional medicine with beneficial effects against inflammation and cancer. We aimed to explore the chemopreventive effect of PZH in colorectal cancer (CRC) through modulating gut microbiota.
METHODS
CRC mouse models were established by azoxymethane plus dextran sulfate sodium treatment or in Apc mice treated with or without PZH (270 mg/kg and 540 mg/kg). Gut barrier function was determined by means of intestinal permeability assays and transmission electron microscopy. Fecal microbiota and metabolites were analyzed by means of metagenomic sequencing and liquid chromatography mass spectrometry, respectively. Germ-free mice or antibiotic-treated mice were used as models of microbiota depletion.
RESULTS
PZH inhibited colorectal tumorigenesis in azoxymethane plus dextran sulfate sodium-treated mice and in Apc mice in a dose-dependent manner. PZH treatment altered the gut microbiota profile, with an increased abundance of probiotics Pseudobutyrivibrio xylanivorans and Eubacterium limosum, while pathogenic bacteria Aeromonas veronii, Campylobacter jejuni, Collinsella aerofaciens, and Peptoniphilus harei were depleted. In addition, PZH increased beneficial metabolites taurine and hypotaurine, bile acids, and unsaturated fatty acids, and significantly restored gut barrier function. Transcriptomic profiling revealed that PZH inhibited PI3K-Akt, interleukin-17, tumor necrosis factor, and cytokine-chemokine signaling. Notably, the chemopreventive effect of PZH involved both microbiota-dependent and -independent mechanisms. Fecal microbiota transplantation from PZH-treated mice to germ-free mice partly recapitulated the chemopreventive effects of PZH. PZH components ginsenoside-F2 and ginsenoside-Re demonstrated inhibitory effects on CRC cells and primary organoids, and PZH also inhibited tumorigenesis in azoxymethane plus dextran sulfate sodium-treated germ-free mice.
CONCLUSIONS
PZH manipulated gut microbiota and metabolites toward a more favorable profile, improved gut barrier function, and suppressed oncogenic and pro-inflammatory pathways, thereby suppressing colorectal carcinogenesis.
Topics: Mice; Animals; Signal Transduction; Gastrointestinal Microbiome; Dextran Sulfate; Phosphatidylinositol 3-Kinases; Apoptosis; Medicine, Traditional; Colorectal Neoplasms; Carcinogenesis; Azoxymethane
PubMed: 37704113
DOI: 10.1053/j.gastro.2023.08.052 -
Frontiers in Cellular and Infection... 2021Gram-positive anaerobic cocci (GPAC) are a commensal part of human flora but are also opportunistic pathogens. This is possibly the first study to report a case of...
Gram-positive anaerobic cocci (GPAC) are a commensal part of human flora but are also opportunistic pathogens. This is possibly the first study to report a case of bacteremia in an abdominal aortic aneurysm (AAA) patient. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) failed to identify the isolate and molecular analysis confirmed it as . A comprehensive literature review revealed that is an emergent pathogen. This study serves as a reminder for practicing clinicians to include anaerobic blood cultures as part of their blood culture procedures; this is particularly important situations with a high level of suspicion of infection factors in some noninfectious diseases, as mentioned in this publication. Clinical microbiologists should be aware that the pathogenic potential of GPAC can be greatly underestimated leading to incorrect diagnosis on using only one method for pathogen identification. Upgradation and correction of the MALDI-TOF MS databases is recommended to provide reliable and rapid identification of GPAC at species level in medical diagnostic microbiology laboratories.
Topics: Aortic Aneurysm; Blood Culture; Firmicutes; Gram-Positive Cocci; Humans; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 35004343
DOI: 10.3389/fcimb.2021.755225 -
Anaerobe Apr 2017Peptoniphilus harei is a Gram-positive anaerobic coccus mainly involved in polymicrobial infections. We report a case of peritoneal infection in a patient with...
Peptoniphilus harei is a Gram-positive anaerobic coccus mainly involved in polymicrobial infections. We report a case of peritoneal infection in a patient with intestinal occlusion. A 48-year-old woman presented with ascitis after an occlusive syndrome. Culture of peritoneal fluid resulted on P. harei isolation. Treatment was performed and resolution of this infection was documented.
Topics: Anti-Bacterial Agents; Ascites; Bacteriological Techniques; Female; Firmicutes; Gram-Positive Bacterial Infections; Humans; Intestinal Obstruction; Laparotomy; Middle Aged; Peritonitis
PubMed: 28286023
DOI: 10.1016/j.anaerobe.2017.03.009 -
Acta Clinica Belgica Feb 2022Peptoniphilus species are Gram-positive anaerobic cocci that are commensals of the human vagina and gut. (Review)
Review
BACKGROUND
Peptoniphilus species are Gram-positive anaerobic cocci that are commensals of the human vagina and gut.
METHODS AND RESULTS
We describe a case of mixed Escherichia coli and Peptoniphilus spp. osteomyelitis identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as Peptoniphilus harei and present a short literature review.
CONCLUSION
To our knowledge, only six cases of P. harei osteomyelitis have been reported to date.
Topics: Escherichia coli; Female; Humans; Osteomyelitis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 32552621
DOI: 10.1080/17843286.2020.1783908 -
Medecine Et Maladies Infectieuses Mar 2018
Topics: Adenocarcinoma; Amoxicillin-Potassium Clavulanate Combination; Bacteria, Anaerobic; Ceftriaxone; Combined Modality Therapy; Drainage; Drug Resistance, Microbial; Fever; Firmicutes; Gram-Positive Bacterial Infections; Gram-Positive Cocci; Humans; Lymph Node Excision; Lymphocele; Male; Middle Aged; Postoperative Complications; Prostatectomy; Prostatic Neoplasms
PubMed: 29153289
DOI: 10.1016/j.medmal.2017.10.003 -
BMJ Case Reports Dec 2019Our case report describes a patient with a common presenting complaint yet an uncommon infection. Our patient presented with a fluctuant breast mass diagnosed as a...
Our case report describes a patient with a common presenting complaint yet an uncommon infection. Our patient presented with a fluctuant breast mass diagnosed as a breast abscess. An aspirate sample was sent for culture and sensitivities, which revealed the presence of and the anaerobe She was therefore prescribed several weeks of amoxicillin and metronidazole, and made a full recovery. There are only three case reports describing as a causative organism for breast abscess, one of which had also occurred in our department. One case also showed the additional presence of Our findings reveal a growing need for increasing clinician awareness of and the importance of aspirate sample culture and sensitivity.
Topics: Abscess; Actinomycetaceae; Adult; Amoxicillin; Anti-Bacterial Agents; Breast Diseases; Female; Firmicutes; Humans; Metronidazole
PubMed: 31796455
DOI: 10.1136/bcr-2019-231194 -
Anaerobe Apr 2012Two groups of previously undescribed anaerobic, gram-positive cocci recovered from human clinical infections were characterized using phenotypic and molecular genotypic...
Two groups of previously undescribed anaerobic, gram-positive cocci recovered from human clinical infections were characterized using phenotypic and molecular genotypic methods. Comparative genotypic analysis showed that the strains within each of these two groups were homogeneous within the group and that each group was unique within the genus Peptoniphilus. The first group is most closely related to Peptoniphilus ivorii and the second group to Peptoniphilus harei. Based on these findings we propose two novel species, Peptoniphilus coxii sp. nov. and Peptoniphilus tyrrelliae sp. nov. The type strains are P. coxii sp. nov., RMA 16757(T) (= JCM 16892(T) = CCUG 59622(T) = ATCC BAA-2106(T)) and P. tyrrelliae sp. nov., RMA 19911(T) (= JCM 16893(T) = CCUG 59621(T) = ATCC BAA-2105(T)).
Topics: Anti-Bacterial Agents; Bacteria, Anaerobic; Bacterial Typing Techniques; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Gram-Positive Bacteria; Gram-Positive Bacterial Infections; Humans; Microbial Sensitivity Tests; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 22178538
DOI: 10.1016/j.anaerobe.2011.11.008 -
MicrobiologyOpen Mar 2019Three previously unidentified Gram-positive anaerobic coccoid bacteria, strains KhD-2 , KHD4 , and Kh-D5 , isolated from a vaginal swab, were characterized using the...
Description of three new Peptoniphilus species cultured in the vaginal fluid of a woman diagnosed with bacterial vaginosis: Peptoniphilus pacaensis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilus vaginalis sp. nov.
Three previously unidentified Gram-positive anaerobic coccoid bacteria, strains KhD-2 , KHD4 , and Kh-D5 , isolated from a vaginal swab, were characterized using the taxonogenomics concept. The phylogenic analysis, phenotypic characteristics, and genotypic data presented in this report attest that these three bacteria are distinct from previously known bacterial species with standing in nomenclature and represent three new Peptoniphilus species. Strain KhD-2 is most closely related to Peptoniphilus sp. DNF00840 and Peptoniphilus harei (99.7% and 98.2% identity, respectively); strain KHD4 to Peptoniphilus lacrimalis (96%) and strain Kh-D5 to Peptoniphilus coxii (97.2%). Strains KhD-2 , KHD4 , and Kh-D5 DNA G+C contents are, respectively, 34.23%, 31.87%, and 49.38%; their major fatty acid was C (41.6%, 32.0%, and 36.4%, respectively). We propose that strains KhD-2 (=CSUR P0125 = DSM 101742), KHD4 (=CSUR P0110 = CECT 9308), and Kh-D5 (=CSUR P2271 = DSM 101839) be the type strains of the new species for which the names Peptoniphilus vaginalis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilu pacaensis sp. nov., are proposed, respectively.
Topics: Adult; Anaerobiosis; Bacterial Typing Techniques; Base Composition; Body Fluids; Cytosol; Fatty Acids; Female; Firmicutes; Humans; Phylogeny; Sequence Homology, Nucleic Acid; Vaginosis, Bacterial
PubMed: 29931836
DOI: 10.1002/mbo3.661 -
Journal of Clinical Microbiology Jun 2007Three groups of previously unknown gram-positive, anaerobic, coccus-shaped bacteria were characterized using phenotypic and molecular taxonomic methods. Phenotypic and...
Three groups of previously unknown gram-positive, anaerobic, coccus-shaped bacteria were characterized using phenotypic and molecular taxonomic methods. Phenotypic and genotypic data demonstrate that these organisms are distinct, and each group represents a previously unknown subline within Clostridium cluster XIII. Two groups are most closely related to Peptoniphilus harei in the genus Peptoniphilus, and the other group is most closely related to Anaerococcus lactolyticus in the genus Anaerococcus. Based on the findings, three novel species, Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov., are proposed. The type strains of Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov. are WAL 10418(T) (= CCUG 53341(T) = ATCC BAA-1383(T)), WAL 12922(T) (= CCUG 53342(T) = ATCC BAA-1384(T)), and WAL 17230(T) (= CCUG 53340(T) = ATCC BAA-1385(T)), respectively.
Topics: Anaerobiosis; Anti-Bacterial Agents; Bacterial Typing Techniques; Genotype; Gram-Positive Bacterial Infections; Gram-Positive Cocci; Humans; Microbial Sensitivity Tests; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 17428937
DOI: 10.1128/JCM.00213-07 -
Cureus Jun 2023Mastitis and breast abscesses are most common in lactating women but can also be observed in non-lactating women, adolescent girls, and neonates. However, breast...
A Case of Pediatric Breast Abscess Caused by Rarely Observed Bacteria in a Three-Year-Old Boy With an Inverted Nipple: Peptoniphilus harei, Actinotignum sanguinis, and Porphyromonas somerae.
Mastitis and breast abscesses are most common in lactating women but can also be observed in non-lactating women, adolescent girls, and neonates. However, breast abscesses are extremely rare in young boys. Herein, we report the case of a three-year-old boy with a swollen and painful right nipple, later diagnosed with a breast abscess. In this case, we suspected that the patient's inverted nipple was the possible site of the infection. To our best knowledge, this is the first case report of breast abscess in a young boy after the neonatal period. Although is the most common pathogen, our patient showed three rare bacteria, namely, , , and , in the culture of the aspirated pus. Furthermore, this case study is the first report of a breast abscess caused by .
PubMed: 37519494
DOI: 10.7759/cureus.41011