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Marine Drugs Dec 2023Chemical epigenetic cultivation of the sponge-derived fungus sp. SWMU-WZ04-1 contributed to the identification of twelve polyketide derivatives, including six new...
Chemical epigenetic cultivation of the sponge-derived fungus sp. SWMU-WZ04-1 contributed to the identification of twelve polyketide derivatives, including six new pestalotiopols E-J (-) and six known analogues (-). Their gross structures were deduced from 1D/2D NMR and HRESIMS spectroscopic data, and their absolute configurations were further established by circular dichroism (CD) Cotton effects and the modified Mosher's method. In the bioassay, the cytotoxic and antibacterial activities of all compounds were evaluated. Chlorinated benzophenone derivatives and exhibited inhibitory effects on and , with MIC values varying from 3.0 to 50 μg/mL. In addition, these two compounds were cytotoxic to four types of human cancer cells, with IC values of 16.2~83.6 μM. The result showed that compound had the probability of being developed into a lead drug with antibacterial ability.
Topics: Humans; Pestalotiopsis; Anti-Bacterial Agents; Bacillus subtilis; Fungi; Polyketides
PubMed: 38248640
DOI: 10.3390/md22010015 -
Microorganisms Jun 2023Species of were mainly introduced as endophytes, plant pathogens or saprobes from various hosts. In this study, ten strains were isolated from , and in China. Based...
Species of were mainly introduced as endophytes, plant pathogens or saprobes from various hosts. In this study, ten strains were isolated from , and in China. Based on multilocus phylogenies from the internal transcribed spacer (ITS), the partial translation elongation factor 1-alpha gene () and the partial beta-tubulin gene (), in conjunction with morphological characteristics, we describe three new species, viz., sp. nov., sp. nov. and sp. nov.
PubMed: 37512800
DOI: 10.3390/microorganisms11071627 -
Journal of Asian Natural Products... Jun 2022A new pair of enantiomeric isoprenylated chromone derivatives, (±)-pestaloficiol X [(±)-], along with a known compound pestaloficiol J (), were isolated from the plant...
A new pair of enantiomeric isoprenylated chromone derivatives, (±)-pestaloficiol X [(±)-], along with a known compound pestaloficiol J (), were isolated from the plant endophytic fungus sp. The racemic mixture was separated through chiral HPLC. The structures of new compounds (±)- were elucidated on the basis of extensive spectroscopic data and their absolute configurations were further configured through computational analysis of their electronic circular dichroism (ECD) spectra. Compound (+)- showed significant inhibitory potency against HL-60 and HEP-3B cell lines, with IC values of 1.35 ± 0.15 and 3.70 ± 0.33 μM, respectively, while compound (-)- showed significant inhibitory potency against HL-60 and HEP-3B cell lines, with IC values of 2.39 ± 0.26 and 2.99 ± 0.35 μM, respectively.
Topics: Antineoplastic Agents; Chromones; Molecular Structure; Pestalotiopsis; Stereoisomerism
PubMed: 34236260
DOI: 10.1080/10286020.2021.1946042 -
Marine Pollution Bulletin Apr 2020The Pestalotiopsis sp. genus comprises filamentous fungi whose species present both pathogenic and clinical-industrial importance. The cultivation and production of...
The Pestalotiopsis sp. genus comprises filamentous fungi whose species present both pathogenic and clinical-industrial importance. The cultivation and production of bivalve molluscs in regions of Rio de Janeiro is lucrative both artisanally and industrially, as the climate, geography and water quality favor the practice of this activity throughout the year at reduced costs, making the region competitive in the national market. The aim of this study was to isolate and identify filamentous fungi associated with the internal tissue anatomy of bivalve molluscs (Perna perna) from mariculture farms. Samples collected from BEMAR marine farms were dissected and transferred to 1% hypochlorite, washed in sterile distilled water and sown on Petri dishes containing potato dextrose agar (BDA). After four days, a white colony, displaying vigorous mycelium, cotton-like with abundant sporulation and black conidia masses was isolated. Observations concerning vegetative and reproductive structures were performed by microcultures stained with Amann's Lactophenol andCotton Blue. Micromorphology analyses indicated spindle and septated conidia, with two to three apical filiform appendages and a short basal pedicel. The result indicates that bivalve mollusks may be bioindicators for the presence of Pestalotiopsis sp; associated with water transport, possibly due to diluted sediments in the medium. No infectious processes or lesions in the processed material were observed. This is, to the best of our knowledge, the first report of Pestalotiopsis sp; in Perna perna mytilids.
Topics: Animals; Aquaculture; Brazil; Food Contamination; Fungi; Humans; Mytilidae; Perna
PubMed: 32275531
DOI: 10.1016/j.marpolbul.2020.110976 -
Plant Disease Aug 2022
Topics: Camellia sinensis; Gene Expression Profiling; Pestalotiopsis; Tea
PubMed: 35728096
DOI: 10.1094/PDIS-12-21-2698-A -
Natural Product Research Jan 2023Two new hydroquinones bearing a 1,3-enyne moiety, pestalotioquinols G and H, together with four known compounds, including pestalotioquinol A, phomonitroester,...
Two new hydroquinones bearing a 1,3-enyne moiety, pestalotioquinols G and H, together with four known compounds, including pestalotioquinol A, phomonitroester, ()-4,6,8-trihydroxy-3,4-dihydronaphthalen-1(2)-one, and scylatone were isolated from the marine fungus (BCC 35283). The structures of these compounds were elucidated by analysis of 2D-NMR and HR-MS data. The known pestalotioquinol A displayed antimalarial activity against K1 with an IC value of 19.0 μM, while pestalotioquinol G displayed weak cytotoxic activity against Vero cell lines with an IC value of 47.9 μM.
Topics: Hydroquinones; Molecular Structure; Fungi; Pestalotiopsis; Plants
PubMed: 34187248
DOI: 10.1080/14786419.2021.1946536 -
Chinese Journal of Natural Medicines Apr 2020Two new caryophyllene-type sesquiterpenoids, pestathenols A (1) and B (2) and one new α-furanone, pestatheranone A (6), along with five known compounds (3-5, 7 and 8)...
Two new caryophyllene-type sesquiterpenoids, pestathenols A (1) and B (2) and one new α-furanone, pestatheranone A (6), along with five known compounds (3-5, 7 and 8) have been isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae. Their structures were unambiguously established by extensive spectroscopic analyses. The absolute configuration of the 5,6-diol moiety in 1 was assigned using Snatzke's method. Compounds 1 and 2 showed weak cytotoxicity against HeLa cell line.
Topics: Furans; HeLa Cells; Humans; Molecular Structure; Pestalotiopsis; Plant Extracts; Polycyclic Sesquiterpenes; Sesquiterpenes
PubMed: 32402402
DOI: 10.1016/S1875-5364(20)30032-7 -
Journal of Basic Microbiology Mar 2023There is an urgent call to search for novel natural compounds against developing multidrug-resistant microorganisms. The present work focuses on the characterization of...
There is an urgent call to search for novel natural compounds against developing multidrug-resistant microorganisms. The present work focuses on the characterization of a plant-associated fungus having bioactivity against methicillin-resistant Staphylococcus aureus (MRSA) strains. A fungal strain P31 was isolated from bark of Dillenia pentagyna and identified as Pestalotiopsis microspora. The maximum anti-MRSA activity was observed from extract of P31 grown in sabouraud dextrose broth. The minimum inhibitory concentrations (MIC) values of P31 extract were 14 μg/ml for methicillin-sensitive S. aureus (MSSA) and 32 μg/ml for MRSA strain, respectively. A crude P31 extract showed strong bactericidal activity by killing all treated MRSA cells within 24 h of treatment at their respective MIC value. A scanning electron microscopic study visualized morphological damage of MRSA cells. The membrane permeability of P31 extract-treated MRSA cells gradually increased which caused release of internal cytoplasmic nucleic acids, proteins and potassium ions (K ) from cells suggesting cell lysis or leakage from cells. A very low concentration of P31 extract was able to inhibit biofilm formed by MRSA cells. Thin layer chromatographic separation followed by gas chromatography-mass spectrometry analysis of the P31 extract revealed a number of antimicrobial compounds along with an anti-MRSA compound 2,4-di-tert-butylphenol. In addition, the P31 extract also showed in-vitro human blood clot lysis activity at various concentrations. The clot lysis activity of P31 extract was found maximum at 500 µg/ml. These findings suggest that fungal isolate P31 has potential as a source of anti-MRSA compounds useful in staph infections.
Topics: Humans; Methicillin-Resistant Staphylococcus aureus; Staphylococcus aureus; Dilleniaceae; Pestalotiopsis; Microbial Sensitivity Tests; Anti-Bacterial Agents
PubMed: 36002312
DOI: 10.1002/jobm.202200294 -
Organic Letters May 2019Cuautepestalorin (4), a 7,8-dihydrochromene-oxoisochromane adduct bearing a spiro-polycyclic (6/6/6/6/6/6) ring system, along with its putative biosynthetic precursors,...
Cuautepestalorin (4), a 7,8-dihydrochromene-oxoisochromane adduct bearing a spiro-polycyclic (6/6/6/6/6/6) ring system, along with its putative biosynthetic precursors, cytosporin M (1), cytosporin N (2), and oxopestalochromane (3), were isolated from the bioactive extract of Pestalotiopsis sp. using a combination of molecular networking and dereplication techniques. Their structures were elucidated using a set of spectroscopic, spectrometric, chiroptical (experimental and theoretical), and X-ray crystallography data. Compounds 3 and 4 exhibited modest potency when evaluated in vitro as α-glucosidase inhibitors.
PubMed: 31033302
DOI: 10.1021/acs.orglett.9b00962 -
MSystems Apr 2022Symbiosis with bacteria is widespread among eukaryotes, including fungi. Bacteria that live within fungal mycelia (endohyphal bacteria) occur in many plant-associated...
Transcriptional Profiles of a Foliar Fungal Endophyte (, Ascomycota) and Its Bacterial Symbiont (, ) Reveal Sulfur Exchange and Growth Regulation during Early Phases of Symbiotic Interaction.
Symbiosis with bacteria is widespread among eukaryotes, including fungi. Bacteria that live within fungal mycelia (endohyphal bacteria) occur in many plant-associated fungi, including diverse Mucoromycota and Dikarya. sp. strain 9143 is a filamentous ascomycete isolated originally as a foliar endophyte of Platycladus orientalis (Cupressaceae). It is infected naturally with the endohyphal bacterium sp. strain 9143, which influences auxin and enzyme production by its fungal host. Previous studies have used transcriptomics to examine similar symbioses between endohyphal bacteria and root-associated fungi such as arbuscular mycorrhizal fungi and plant pathogens. However, currently there are no gene expression studies of endohyphal bacteria of Ascomycota, the most species-rich fungal phylum. To begin to understand such symbioses, we developed methods for assessing gene expression by sp. and sp. when grown in coculture and when each was grown axenically. Our assays showed that the density of sp. in coculture was greater than in axenic culture, but the opposite was true for sp. Dual-transcriptome sequencing (RNA-seq) data demonstrate that growing in coculture modulates developmental and metabolic processes in both the fungus and bacterium, potentially through changes in the balance of organic sulfur via methionine acquisition. Our analyses also suggest an unexpected, potential role of the bacterial type VI secretion system in symbiosis establishment, expanding current understanding of the scope and dynamics of fungal-bacterial symbioses. Interactions between microbes and their hosts have important outcomes for host and environmental health. Foliar fungal endophytes that infect healthy plants can harbor facultative endosymbionts called endohyphal bacteria, which can influence the outcome of plant-fungus interactions. These bacterial-fungal interactions can be influential but are poorly understood, particularly from a transcriptome perspective. Here, we report on a comparative, dual-RNA-seq study examining the gene expression patterns of a foliar fungal endophyte and a facultative endohyphal bacterium when cultured together versus separately. Our findings support a role for the fungus in providing organic sulfur to the bacterium, potentially through methionine acquisition, and the potential involvement of a bacterial type VI secretion system in symbiosis establishment. This work adds to the growing body of literature characterizing endohyphal bacterial-fungal interactions, with a focus on a model facultative bacterial-fungal symbiosis in two species-rich lineages, the Ascomycota and .
Topics: Symbiosis; Endophytes; Pestalotiopsis; Gammaproteobacteria; Type VI Secretion Systems; Ascomycota; Bacteria; Fungi, Unclassified; Xanthomonadaceae; Plants; Methionine
PubMed: 35293790
DOI: 10.1128/msystems.00091-22