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Plant Disease Jan 2018Common guava (Psidium guajava L.) is a fruit tree of global economic importance. It is grown in Asia, South and Central America, and Hawaii for its exquisite aroma and...
Common guava (Psidium guajava L.) is a fruit tree of global economic importance. It is grown in Asia, South and Central America, and Hawaii for its exquisite aroma and flavor, and nutritional and medical properties. However, guava production is limited by guava scab, caused by fungi in the Pestalotiopsis genus. Characteristic symptoms of guava scab are corky, ovoid or round lesions on fruit surfaces. These lesions may thicken, affecting the flesh below and reducing fruit quality and commercial value. We characterized 81 isolates isolated from guava scab lesions on guava leaves and fruit in different regions of Colombia, and identified them as Pestalotiopsis and Neopestalotiopsis spp. We analyzed the morphology, pathogenicity, and genetic diversity of the isolates based on the sequences of the internal transcribed spacer, β-tubulin, and elongation factor genes. Isolates were morphologically, pathogenically, and genetically diverse but the diversity did not correlate with geographical origin, or guava cultivar or tissue from which the isolates were recovered. Selected monosporic isolates included in the multiple-gene analysis were identified as belonging to two genera: Neopestalotiopsis (65 isolates with versicolorous conidia) and Pestalotiopsis (4 isolates with concolorous conidia).
Topics: Colombia; Fruit; Genes, Fungal; Genetic Variation; Plant Diseases; Plant Leaves; Psidium; Sequence Analysis, DNA; Xylariales
PubMed: 30673452
DOI: 10.1094/PDIS-01-17-0068-RE -
Molecular Plant-microbe Interactions :... May 2022
Topics: Camellia sinensis; MicroRNAs; Pestalotiopsis; Plant Leaves; Tea
PubMed: 35179950
DOI: 10.1094/MPMI-10-21-0262-A -
MycoKeys 2024, commonly referred to as pestalotiopsis-like fungi, exhibit a broad distribution and are frequently found as endophytes, saprobes and pathogens across various plant...
, commonly referred to as pestalotiopsis-like fungi, exhibit a broad distribution and are frequently found as endophytes, saprobes and pathogens across various plant hosts. The taxa within pestalotiopsis-like fungi are classified into three genera viz. , and , based on the conidial colour of their median cells and multi-locus molecular phylogenies. In the course of a biodiversity investigation focusing on pestalotiopsis-like fungi, a total of 12 fungal strains were identified. These strains were found to be associated with stromata of , and in various regions of Taiwan from 2018 to 2021. These strains were evaluated morphologically and multi-locus phylogenetic analyses of the ITS (internal transcribed spacer), (translation elongation factor 1-α) and (beta-tubulin) gene regions were conducted for genotyping. The results revealed seven well-classified taxa and one tentative clade in and . One novel species, and four new records, , , and , were reported for the first time in Taiwan. In addition, and an unclassified strain of were identified, based on similarities of phylogeny and morphology. However, the data obtained in the present study suggest that the currently recommended loci for species delimitation of pestalotiopsis-like fungi do not deliver reliable or adequate resolution of tree topologies. The mycelial growth rates of selected strains from these taxa had an optimum temperature of 25 °C, but growth ceased at 5 °C and 35 °C, while all the strains grew faster under alkaline than acidic or neutral pH conditions. This study provides the first assessment of pestalotiopsis-like fungi, associated with entomopathogenic taxa.
PubMed: 38333551
DOI: 10.3897/mycokeys.101.113090 -
The Journal of Antibiotics Nov 2018Secondary metabolites of microorganisms have proven to be an excellent source of drugs. We isolated a new antibiotic, named pestiocandin (1), from a culture broth of a...
Secondary metabolites of microorganisms have proven to be an excellent source of drugs. We isolated a new antibiotic, named pestiocandin (1), from a culture broth of a filamentous fungus, Pestalotiopsis humus FKI-7473, using a multidrug-sensitive budding yeast, S. cerevisiae 12geneΔ0HSR-iERG6. The structure of 1 was elucidated by various NMR studies. All geometric isomerisms of 1 were shown to be the E-form and two pyranose units of 1 were found to be glucose and galactose types. Compound 1 showed weak growth inhibition against Gram-positive and Gram-negative bacteria, yeasts and a filamentous fungus. It displayed more potent growth inhibition against multidrug-sensitive yeasts than wild-type yeasts.
Topics: Aminoglycosides; Anti-Bacterial Agents; Antifungal Agents; Ascomycota; Drug Resistance, Fungal; Echinocandins; Gram-Negative Bacteria; Gram-Positive Bacteria; Magnetic Resonance Spectroscopy; Microbial Sensitivity Tests; Saccharomyces cerevisiae; Stereoisomerism; Yeasts
PubMed: 30232378
DOI: 10.1038/s41429-018-0102-7 -
The Journal of Antibiotics Aug 2020Three new carboxylic acid derivatives, pestallic acids F and G (1 and 2), pestalotiopyrone N (3), and a new diphenylketone derivative named neopestalone (5) were...
Three new carboxylic acid derivatives, pestallic acids F and G (1 and 2), pestalotiopyrone N (3), and a new diphenylketone derivative named neopestalone (5) were obtained from the liquid cultures of marine alga-derived endophytic fungus Pestalotiopsis neglecta SCSIO41403, along with six known compounds (4, 6-10). The structures of those new compounds were elucidated mainly by analysis of their NMR and MS data. The isolated compounds were evaluated for their anti-Dengue virus and COX-2 inhibitory activities, and two diphenylketone derivatives (5 and 6) exhibited obvious COX-2 inhibitory activities, with the IC values being 5.8 and 3.4 μM, respectively.
Topics: Antiviral Agents; Aquatic Organisms; Carboxylic Acids; Cyanobacteria; Cyclooxygenase 2 Inhibitors; Dengue Virus; Endophytes; Ketones; Neglecta; Pestalotiopsis
PubMed: 32286514
DOI: 10.1038/s41429-020-0308-3 -
Journal of Natural Products Nov 2020Seven new β-caryophyllene derivatives, pestalotiphains A-G (-), along with six known analogues (-), were isolated from the plant-associated . Compound represents the...
Seven new β-caryophyllene derivatives, pestalotiphains A-G (-), along with six known analogues (-), were isolated from the plant-associated . Compound represents the first example of a caryophyllene-adenine hybrid, and contains a novel oxatricyclo[4.3.1.0] system. Their structures and absolute configurations were assigned by interpretation of a combination of spectroscopic data and electronic circular dichroism calculations. Compound exhibited moderate inhibition of HL-60 and THP-1 cell lines (IC, 6.2 and 2.0 μM, respectively). A candidate biosynthetic gene cluster responsible for these compounds was uncovered by bioinformatics analyses and confirmed by a biochemical approach.
Topics: Biosynthetic Pathways; Cell Line, Tumor; Drug Screening Assays, Antitumor; Genes, Fungal; Humans; Multigene Family; Oxygen; Pestalotiopsis; Phylogeny; Polycyclic Sesquiterpenes; Spectrum Analysis
PubMed: 33064488
DOI: 10.1021/acs.jnatprod.0c00501 -
Journal of Fungi (Basel, Switzerland) May 2023Bayberry twig blight caused by the ascomycete fungus is a devastating disease threatening worldwide bayberry production. However, the molecular basis underlying the...
Bayberry twig blight caused by the ascomycete fungus is a devastating disease threatening worldwide bayberry production. However, the molecular basis underlying the pathogenesis of is largely unknown. Here, we identified and functionally characterized the MAP kinase PvMk1 in through genetic and cellular biochemical approaches. Our analysis reveals a central role of PvMk1 in regulating virulence on bayberry. We demonstrate that PvMk1 is involved in hyphal development, conidiation, melanin biosynthesis, and cell wall stress responses. Notably, PvMk1 regulates autophagy and is essential for hyphal growth under nitrogen-depleting conditions. These findings suggest the multifaceted role of PvMk1 in regulating development and virulence. More remarkably, this evidence of virulence-involved cellular processes regulated by PvMk1 has paved a fundamental way for further understanding the impact of pathogenesis on bayberry.
PubMed: 37367542
DOI: 10.3390/jof9060606 -
Pesticide Biochemistry and Physiology May 2018Dendrobium nobile (D. nobile) is a valuable Chinese herbal medicine. The discovery of microbial resources from has provided a wealth of raw materials. Stalk rot, which...
Dendrobium nobile (D. nobile) is a valuable Chinese herbal medicine. The discovery of microbial resources from has provided a wealth of raw materials. Stalk rot, which is caused by Pestalotiopsis, is one of the most serious diseases of D nobile and has resulted in serious losses in production. However, an effective method for the prevention and control of stalk rot remains lacking. In this study, we aimed to identify a biocontrol strain against Pestalotiopsis. We isolated Paenibacillus polymyxa Y-1, an endophytic bacterium, from the stem of D. nobile. Three pairs of active metabolites isolated from this bacterium were identified as fusaricidin compounds. We then investigated the mechanism of fusaricidin compounds on Pestalotiopsis via proteomics. Proteomics data showed that the compounds mainly inhibit energy generation in the respiratory chain and amino acid biosynthesis of Pestalotiopsis.
Topics: Amino Acids; Antifungal Agents; Ascomycota; Bacterial Proteins; China; DNA, Fungal; Dendrobium; Electron Transport; Energy Metabolism; Paenibacillus polymyxa; Plant Diseases; Proteomics; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic
PubMed: 29933995
DOI: 10.1016/j.pestbp.2017.08.012 -
Molecules (Basel, Switzerland) Jan 2020Two new xanthone derivatives, pestalotiones A () and B (), one new diphenyl ketone riboside, pestalotione C (), and one new diphenyl ether, pestalotione D (), along with...
Two new xanthone derivatives, pestalotiones A () and B (), one new diphenyl ketone riboside, pestalotione C (), and one new diphenyl ether, pestalotione D (), along with five known compounds isosulochrin dehydrate (), 3,8-dihydroxy-6-methyl-9-oxo-9-xanthene-1-carboxylate (), isosulochrin (), chloroisosulochrin (), and pestalotether D (), were isolated from the crude extract of the plant endophytic fungus (N635). The structures of the new compounds were unambiguously deduced by HRESIMS and 1D/2D-NMR spectroscopic data. Compound showed modest cytotoxicity against the HeLa cell line with an IC value of 35.2 μM. Compound also showed cytotoxic to the HeLa and MCF-7 cell lines, with IC values of 60.8 and 22.6 μM, respectively. Additionally, compounds and exhibited antioxidant activity in scavenging DPPH radical with IC values of 54.2 and 59.2 μg/mL, respectively.
Topics: Antioxidants; Benzoates; Biphenyl Compounds; Cell Survival; Endophytes; HeLa Cells; Humans; Hydrocarbons, Chlorinated; Inhibitory Concentration 50; MCF-7 Cells; Magnetic Resonance Spectroscopy; Molecular Structure; Picrates
PubMed: 31979166
DOI: 10.3390/molecules25030470 -
Phytochemistry Nov 2020Four undescribed pyrone derivatives, pestalopyrones A-D, containing unusual tricyclic 5/6/6 polycyclic skeletons, were isolated from the ethyl acetate extract of the...
Four undescribed pyrone derivatives, pestalopyrones A-D, containing unusual tricyclic 5/6/6 polycyclic skeletons, were isolated from the ethyl acetate extract of the endophytic fungus Pestalotiopsis neglecta S3 derived from the fresh stems of Rubia podantha Diels (Rubiaceae). Their planar structures were elucidated mainly by NMR and HRESIMS. Pestalopyrones A-D contained six contiguous chiral carbons, and the relative configurations of C-4, C-5, and C-8 in tricyclic 5/6/6 polycyclic skeletons were determined by ROESY spectra. For pestalopyrone B, the absolute configuration of C-16 was determined by the Mosher's method. All isolated compounds were tested for their cytotoxicity against cancer cell lines, antibacterial activity, and inhibitory effect on the lipopolysaccharide (LPS)-induced nitric oxide (NO) production, and the results showed that pestalopyrone A inhibited LPS-induced NO production with an IC value of 35.8 μM.
Topics: Molecular Structure; Pyrones; Xylariales
PubMed: 32919290
DOI: 10.1016/j.phytochem.2020.112505