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Clinical Chemistry Aug 1980We describe a simple, sensitive, and reliable radioimmunoassay for prednisolone and prednisolone-21-hemisuccinate in serum. The antiserum produced in rabbits to...
We describe a simple, sensitive, and reliable radioimmunoassay for prednisolone and prednisolone-21-hemisuccinate in serum. The antiserum produced in rabbits to prednisolone-21-hemisuccinate/bovine serum albumin was specific for prednisolone and prednisolone-21-hemisuccinate. A simple dichloromethane extraction permitted the separation of prednisolone from prednisolone-21-hemisuccinate in the serum samples. Interference by cortisol, although not insignificant, is minimized in this assay. We used the method to measure prednisolone and prednisolone-21-hemisuccinate concentrations after a bolus injection of prednisolone-21-hemisuccinate into human beings and mice.
Topics: Animals; Cerebrovascular Disorders; Cross Reactions; Humans; Male; Mice; Prednisolone; Radioimmunoassay
PubMed: 7398044
DOI: No ID Found -
Gut Aug 1985A double blind controlled trial has been conducted in 40 patients to compare the therapeutic effects of prednisolone metasulphobenzoate enemas with those of... (Clinical Trial)
Clinical Trial Comparative Study
A double blind controlled trial has been conducted in 40 patients to compare the therapeutic effects of prednisolone metasulphobenzoate enemas with those of prednisolone-21-phosphate enemas. Both enemas brought about improvement in symptoms and sigmoidoscopic appearances in more than 70% of patients treated. The absorption of prednisolone from the metasulphobenzoate enema in three patients was less than from the 21-phosphate enema. In view of the low plasma prednisolone concentrations obtained, there are theoretical advantages in using a poorly absorbed enema to avoid the possibility of systemic steroid effects in patients requiring long term steroid treatment.
Topics: Adult; Aged; Clinical Trials as Topic; Colitis, Ulcerative; Double-Blind Method; Enema; Female; Humans; Intestinal Absorption; Male; Middle Aged; Prednisolone
PubMed: 3894171
DOI: 10.1136/gut.26.8.822 -
Praxis Jun 2009
Topics: Acyclovir; Anti-Inflammatory Agents; Antiviral Agents; Bell Palsy; Drug Administration Schedule; Drug Therapy, Combination; Follow-Up Studies; Humans; Prednisolone; Prognosis; Randomized Controlled Trials as Topic
PubMed: 19513976
DOI: 10.1024/1661-8157.98.12.655 -
HNO Jul 2001Prednisolone is the drug of first choice for the treatment of cochleovestibular disorders, such as sudden hearing loss. Because of the known side effects, the efficient...
[Permeability of the round window membrane for prednisolone-21-hydrogen succinate. Prednisolone content of the perilymph after local administration vs. systemic injection].
BACKGROUND AND OBJECTIVE
Prednisolone is the drug of first choice for the treatment of cochleovestibular disorders, such as sudden hearing loss. Because of the known side effects, the efficient drug levels to be achieved within inner ear fluids are limited by intravenous administration. The aim of the study was to determine the concentration in the perilymph of prednisolone-21-hydrogen succinate applied into the round window niche in comparison to the concentration after intraperitoneal application.
METHODS
Application of prednisolone-21-hydrogen succinate (5 mg in 0.1 ml) on the round window membrane was performed after sedation under microscopic view directly into the round window niche of the guinea pig. In order to compare the results, perilymph samples after systemic application of 60 mg/kg body weight prednisolone were used. The time between application and taking specimens of perilymph from the cochlea varied. Specimens of perilymph were obtained after 15, 20, 80, 180, 330, and 960 min (10 specimens in each group, n = 60) by dissecting the cochlea and opening the apex cochleae. Levels of prednisolone-21-hydrogen succinate in perilymph were measured by isocratic high-pressure liquid chromatography (HPLC).
RESULTS
The highest levels of prednisolone-21-hydrogen succinate were found after 180 min: 952.3 mg/l (95% confidence interval: 382.7). After 960 min the level was 18.72 mg/l (95% confidence interval: 16.9). In the group with systemic application, the levels measured were below 14.71 mg/l (95% confidence interval: 7.05).
CONCLUSION
The results demonstrate that high levels of prednisolone-21-hydrogen succinate in perilymph are achievable by local application of a single dose into the round window niche. After application of 5 mg, the levels of prednisolone are measurable up to 16 h.
Topics: Administration, Topical; Animals; Biological Availability; Guinea Pigs; Injections, Intraperitoneal; Metabolic Clearance Rate; Perilymph; Prednisolone; Round Window, Ear
PubMed: 11486587
DOI: 10.1007/s001060170078 -
Transactions of the St. John's Hospital... 1960
Topics: Dermatology; Methylprednisolone; Prednisolone; Skin Diseases
PubMed: 13706431
DOI: No ID Found -
Gastroenterologie Clinique Et Biologique 1985In order to determine whether oral prednisolone efficiency in inflammatory bowel disease may be limited by impaired absorption, seven patients were studied. These were...
In order to determine whether oral prednisolone efficiency in inflammatory bowel disease may be limited by impaired absorption, seven patients were studied. These were children with active disease, localized or extensive on the small and/or the large bowel. Serum prednisolone concentrations were compared within each subject after oral and intravenous administration of the same dose. Absorption was found complete, and its rate similar to that in nine normal adults. Thus prednisolone malabsorption did not limit treatment efficiency. In one patient, an alternative explanation was poor compliance with the treatment. Less complete studies in nine other children with active inflammatory bowel disease suggested the same conclusion. Prednisolone malabsorption in children with inflammatory bowel disease, if it exists, could only be transitory or exceptional.
Topics: Administration, Oral; Adolescent; Adult; Child; Child, Preschool; Enterocolitis; Female; Humans; Infusions, Parenteral; Intestinal Absorption; Kinetics; Male; Prednisolone
PubMed: 4076715
DOI: No ID Found -
Lakartidningen
Topics: Bell Palsy; Evidence-Based Medicine; Glucocorticoids; Humans; Prednisolone; Prognosis
PubMed: 19025152
DOI: No ID Found -
Journal of Chromatography May 1982We describe the first method of routine measurement of prednisone, prednisolone and 6 beta-hydroxyprednisolone concomitantly in urine. Urine (3 ml) is extracted with...
Simultaneous measurement of prednisone, prednisolone and 6 beta-hydroxyprednisolone in urine by high-performance liquid chromatography coupled with a radioactivity detector.
We describe the first method of routine measurement of prednisone, prednisolone and 6 beta-hydroxyprednisolone concomitantly in urine. Urine (3 ml) is extracted with ethyl acetate, washed with base and separated by high-performance liquid chromatography on a silica column with a solvent system of hexane--diethyl ether--ethanol--tetrahydrofuran--glacial acetic acid (59.9:31:2.3:6.5:0.3, v/v). The steroids are detected at 254 nm. Because no conventional internal standard was found, 6 beta-[3H]hydroxycortisol and [3H]prednisolone are added to urine prior to extraction; 3H is monitored by a radioactivity detector coupled with the chromatograph. The assay exhibits linearity from 200 to 7500 ng and an inter-day variability of less than 11.4% (C.V.).
Topics: Adult; Chromatography, High Pressure Liquid; Evaluation Studies as Topic; Humans; Injections, Intravenous; Prednisolone; Prednisone; Reference Values
PubMed: 7096466
DOI: 10.1016/s0378-4347(00)84270-x -
British Journal of Rheumatology Aug 1986Forty patients with frozen shoulder were randomly allocated to a treated group who received oral prednisolone or a nontreatment group who received no specific therapy.... (Clinical Trial)
Clinical Trial Randomized Controlled Trial
Forty patients with frozen shoulder were randomly allocated to a treated group who received oral prednisolone or a nontreatment group who received no specific therapy. All the patients were encouraged to perform home pendular exercise. The improvement in pain at night showed a significantly different pattern in the two groups (p less than 0.05), with the treated group improving more rapidly. Pain on movement and at rest and the recovery in the range of movement was not significantly different in the two groups. There was no difference between the patient groups at the end of the study (8 months follow-up) and patients still had a marked restriction in range (p less than 0.0001) when compared to normal controls of similar age and sex.
Topics: Administration, Oral; Humans; Joint Diseases; Prednisolone; Shoulder Joint
PubMed: 3730737
DOI: 10.1093/rheumatology/25.3.288 -
The Journal of Investigative Dermatology Apr 1960
Topics: Methylprednisolone; Prednisolone; Psoriasis; Triamcinolone
PubMed: 13810959
DOI: 10.1038/jid.1960.44