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Journal of Cataract and Refractive... Jul 2022To establish whether difluprednate 0.05% nanoemulsion (DIFL) twice a day is as effective as prednisolone acetate 1% + phenylephrine hydrochloride 0.12% suspension (PRED)... (Randomized Controlled Trial)
Randomized Controlled Trial
PURPOSE
To establish whether difluprednate 0.05% nanoemulsion (DIFL) twice a day is as effective as prednisolone acetate 1% + phenylephrine hydrochloride 0.12% suspension (PRED) 4 times a day for postsurgical inflammation treatment.
SETTING
4 private Argentine ophthalmological centers.
DESIGN
Noninferiority, prospective, multicenter, double-blind, randomized, parallel-group, comparative trial.
METHODS
A total of 259 patients who underwent phacoemulsification randomly received DIFL or PRED, starting the day before surgery and continuing for 28 days. The primary endpoint was central corneal thickness. Noninferior anti-inflammatory efficacy was considered if the difference of corneal thickness between baseline and day 4 did not differ beyond 17 μm between treatments. Secondary endpoints were cell and flare, corrected distance visual acuity (CDVA), endothelial cell count, optical coherence tomography (OCT) central macular thickness, and intraocular pressure. All outcomes were evaluated at baseline and day 1, 4, and 28 postoperatively.
RESULTS
225 patients finished the study. The difference in corneal thickness at baseline and day 4 did not differ beyond 17 μm between treatments (95% CI -2.78 μm to 14.84 μm), with no statistically significant difference ( P = .523). No statistically significant differences were found between groups in total anterior chamber clearance at any study timepoint ( P > .05). Moreover, no statistically significant differences were reported between treatments in CDVA ( P = .455), endothelial cell count ( P = .811), OCT central macular thickness ( P = .869), and intraocular pressure outcome ( P = .316).
CONCLUSIONS
Difluprednate administered twice a day was at least as effective as prednisolone acetate administered 4 times a day for inflammatory treatment after cataract surgery.
Topics: Cataract; Eye Diseases; Fluprednisolone; Humans; Inflammation; Phacoemulsification; Postoperative Complications; Prednisolone; Prospective Studies
PubMed: 34759176
DOI: 10.1097/j.jcrs.0000000000000863 -
British Medical Journal Jun 1979
Topics: Acute Disease; Humans; Intestinal Absorption; Intestinal Obstruction; Male; Middle Aged; Prednisolone; Tablets, Enteric-Coated
PubMed: 466230
DOI: 10.1136/bmj.1.6180.1795-c -
Journal of Mass Spectrometry : JMS Mar 2015Glucocorticosteroids are prohibited in sports when used by systemic administrations (e.g. oral), whereas they are allowed using other administration ways. Strategies to...
Glucocorticosteroids are prohibited in sports when used by systemic administrations (e.g. oral), whereas they are allowed using other administration ways. Strategies to discriminate between administrations routes have to be developed by doping control laboratories. For this reason, the metabolism of prednisolone (PRED) was studied using liquid chromatography coupled to tandem mass spectrometry. A single oral (10 mg) dose of PRED was administered to two healthy male volunteers. Urine samples were collected up to 6 days after administration. Samples were hydrolyzed with β-glucuronidase and subjected to liquid-liquid extraction with ethyl acetate in alkaline conditions. The extracts were analyzed by liquid chromatography coupled to tandem mass spectrometry. Precursor ion scan methods (m/z 77, 91, 105, 121, 147 and 171) in positive ionization and neutral loss scan methods (76 and 94 Da) in negative ionization modes were applied for the open detection of PRED metabolites. Using these methods, PRED parent compound plus 20 metabolites were detected. PRED and 11 metabolites were characterized by comparison with standards of the compounds (PRED, prednisone, 20β-dihydro-PRED and 20α-dihydro-PRED, 20β-dihydro-prednisone and 20α-dihydro-prednisone, 6β-hydroxy-PRED and 6α-hydroxy-PRED, 20β isomers and 20α isomers of 6β,11β,17α,20,21-pentahydroxypregnan-1,4-diene-3-one, 6α,11β,17α,20β,21-pentahydroxypregnan-1,4-diene-3-one and Δ(6) -PRED). Using mass spectrometric data, feasible structures were proposed for seven of the remaining nine detected metabolites, including several 6-hydroxy-metabolites. Eleven of the characterized metabolites have not been previously described. Maximum excretion rates for PRED metabolites were achieved in first 24 h; however, most of the metabolites were still detectable in the last collected samples (day 6).
Topics: Adult; Chromatography, Liquid; Doping in Sports; Humans; Male; Molecular Weight; Prednisolone; Tandem Mass Spectrometry; Young Adult
PubMed: 25800201
DOI: 10.1002/jms.3571 -
American Journal of Veterinary Research Mar 1985The pharmacokinetics of prednisolone were studied in a group of 6 cows given prednisolone 21-sodium succinate IV and IM (600 micrograms/kg of body weight expressed as... (Comparative Study)
Comparative Study
The pharmacokinetics of prednisolone were studied in a group of 6 cows given prednisolone 21-sodium succinate IV and IM (600 micrograms/kg of body weight expressed as prednisolone alcohol) and prednisolone acetate IM (600 micrograms/kg of body weight expressed as prednisolone alcohol). After IV administration of prednisolone 21-sodium succinate, the half-life of elimination was 3.6 +/- 1.177 hours. After IM administration of prednisolone 21-sodium succinate, absorption was rapid and complete. After IM administration of prednisolone acetate, absorption was very slow with an absorption half-life of 48 hours, but was still complete. Basal plasma hydrocortisone was about 7.5 ng/ml. After IV and IM administration of prednisolone 21-sodium succinate, plasma hydrocortisone returned to normal values within 48 hours. In contrast, after IM administration of prednisolone acetate, a long adrenal suppression lasting from 4 to 6 weeks was observed.
Topics: Adrenal Glands; Animals; Cattle; Chromatography, High Pressure Liquid; Female; Half-Life; Hydrocortisone; Injections, Intramuscular; Injections, Intravenous; Prednisolone
PubMed: 3994138
DOI: No ID Found -
Collegium Antropologicum Jan 2007134 patients with Age-related Macular Degeneration (AMD) (aging 47-75 years) were treated in therapy procedure with parabulbar injections of Methylprednisolone Acetate... (Clinical Trial)
Clinical Trial
134 patients with Age-related Macular Degeneration (AMD) (aging 47-75 years) were treated in therapy procedure with parabulbar injections of Methylprednisolone Acetate and Prednisolone Acetate. In the first group of patients with AMD (n = 71 patients) were treated with Methylprednisolone acetate, and second group (n = 63 patients) with Prednisolone acetate. Each patient was given doses of 60 mg, through two weeks, 10 mg every second day. It's estimated in all patients ameliorate in macular threshold, so that it's in the group with Methylprednisolone treatment, ameliorate effect begins after first week, than in second group, treated with Prednisolone, initial ameliorate effect is after second week. Complete effect in both groups is after 2 months. It can be concluded that the treatment of AMD with glucocorticoids has the ameliorate effect in vision loss and it is decided that earlier effect in the group treated with Methylprednisolone, is probably of higher affinity for glucocorticoid receptors.
Topics: Aged; Glucocorticoids; Humans; Macular Degeneration; Methylprednisolone; Methylprednisolone Acetate; Middle Aged; Neuroprotective Agents; Prednisolone
PubMed: 17469755
DOI: No ID Found -
Journal of Pharmaceutical Sciences Nov 1981An animal model was sought that would mimic humans with regard to the dose-dependent pharmacokinetics of prednisolone. Four rabbits were each given 0.5 and 10 mg iv of...
An animal model was sought that would mimic humans with regard to the dose-dependent pharmacokinetics of prednisolone. Four rabbits were each given 0.5 and 10 mg iv of prednisolone, and timed blood samples were obtained. Plasma prednisolone and prednisone concentrations were assayed by high-performance liquid chromatography, and protein binding was assessed using equilibrium dialysis at 37 degrees. Increases in the systemic clearance, volume of distribution at steady state, mean residence time (in three of four rabbits), and variance of residence time occurred as dose was increased. As in humans, prednisolone was partly converted to prednisone in the rabbit. Transcortin and albumin concentrations and their affinity constants for binding prednisolone were also similar to humans.
Topics: Animals; Blood Proteins; Prednisolone; Protein Binding; Rabbits; Time Factors; Tissue Distribution
PubMed: 7299663
DOI: 10.1002/jps.2600701105 -
La Prensa Medica Mexicana May 1960
Topics: Dermatology; Methylprednisolone; Prednisolone
PubMed: 13741115
DOI: No ID Found -
Journal of Clinical Pharmacy and... Oct 1990The stability of prednisolone and prednisolone acetate has been investigated at 37 degrees C in seven semi-solid vehicles, including almond oil, beeswax, Cremophor RH... (Comparative Study)
Comparative Study
The stability of prednisolone and prednisolone acetate has been investigated at 37 degrees C in seven semi-solid vehicles, including almond oil, beeswax, Cremophor RH 40, Lanette N, and hydrogel formulations of hydroxy-propylcellulose, carbomer and bentonite. Extraction methods and a high-performance liquid chromatographic (HPLC) procedure have been developed in order to assay the content of undegraded steroid at time 0 and after 28 days. Prednisolone acetate showed superior stability compared to prednisolone. Bentonite induced considerable degradation of both steroid compounds, whereas the two absorption bases, Cremophor RH 40 and Lanette N, affected mainly the stability of prednisolone. In the carbomer hydrogel, prednisolone acetate hydrolysed to a small extent during the trituration process. The most effective steroid stability was found in the two lipophilic bases and the hydroxypropylcellulose hydrogel.
Topics: Administration, Topical; Chromatography, High Pressure Liquid; Drug Stability; Gels; Pharmaceutical Vehicles; Prednisolone
PubMed: 2289934
DOI: 10.1111/j.1365-2710.1990.tb00392.x -
Wound Repair and Regeneration :... 2006Macrophages play an important role in the inflammatory phase of wound healing and their activity regulates fibroblasts and keratinocytes. Modulation of macrophage...
Macrophages play an important role in the inflammatory phase of wound healing and their activity regulates fibroblasts and keratinocytes. Modulation of macrophage function may result in improvement of the wound healing process. Prednisolone phosphate (PLP) encapsulated into liposomes was administered to partial-thickness wounds in rats. A single dose of 75 microg/kg, applied directly after wounding, resulted in up to a 30% reduction of wound contraction at 28 days after wounding. This effect could not be achieved in the group that was administered free PLP or liposomes containing phosphate-buffered saline to the wound. The number of myofibroblasts was up to 50% lower in wounds treated with the liposomal PLP at 4 days after wounding. The number of macrophages present in the wounds was not statistically different between groups. Most probably, the production of cytokines and growth factors by macrophages is altered after phagocytosing the liposomes, resulting in reduced wound contraction.
Topics: Administration, Topical; Analysis of Variance; Animals; Disease Models, Animal; Fibroblasts; Immunohistochemistry; Liposomes; Male; Prednisolone; Rats; Rats, Wistar; Wound Healing
PubMed: 17014673
DOI: 10.1111/j.1743-6109.2006.00167.x -
Journal of Visualized Experiments : JoVE Oct 2018Zebrafish are able to regenerate various organs, including appendages (fins) after amputation. This involves the regeneration of bone, which regrows within roughly two...
Zebrafish are able to regenerate various organs, including appendages (fins) after amputation. This involves the regeneration of bone, which regrows within roughly two weeks after injury. Furthermore, zebrafish are able to heal bone rapidly after trepanation of the skull, and repair fractures that can be easily introduced into zebrafish bony fin rays. These injury assays represent feasible experimental paradigms to test the effect of administered drugs on rapidly forming bone. Here, we describe the use of these 3 injury models and their combined use with systemic glucocorticoid treatment, which exerts bone inhibitory and immunosuppressive effects. We provide a workflow on how to prepare for immunosuppressive treatment in adult zebrafish, illustrate how to perform fin amputation, trepanation of calvarial bones, and fin fractures, and describe how the use of glucocorticoids affects both bone forming osteoblasts and cells of the monocyte/macrophage lineage as part of innate immunity in bone tissue.
Topics: Animals; Disease Models, Animal; Prednisolone; Regeneration; Zebrafish
PubMed: 30394396
DOI: 10.3791/58429