-
Nihon Rinsho. Japanese Journal of... Sep 2002
Review
Topics: Antigen-Antibody Complex; Humans; Hyperplasia; Hypersensitivity, Delayed; Immunoglobulin M; Inclusion Bodies; Lymph Nodes; Macrophages; Propionibacteriaceae; Sarcoidosis
PubMed: 12434785
DOI: No ID Found -
International Journal of Systematic and... Jul 2020A novel, facultatively anaerobic actinobacterium, designated strain CBA3103, was isolated from sediment of the Geum River in South Korea. Phylogenetic analysis indicated...
A novel, facultatively anaerobic actinobacterium, designated strain CBA3103, was isolated from sediment of the Geum River in South Korea. Phylogenetic analysis indicated that strain CBA3103 is most closely related to LZ-22 (98.47 % 16S rRNA gene sequence similarity). The genome of strain CBA3103 was 3 649 865 bp with a DNA G+C content of 69.6 mol%. The average nucleotide identity value between strain CBA3103 and LZ-22 was 79.22 %. Cells of strain CBA3103 were Gram-positive, rod-shaped, 0.6-0.9 µm wide and 1.4-2.4 µm long. Growth occurred at 15-40 °C (optimum, 35 °C), at pH 6.0-7.0 (optimum, pH 7.0) and with 0-2 % NaCl (w/v) (optimum, 0-1 %, w/v). The major cellular fatty acids in strain CBA3103 were anteiso-C, anteiso-C A and iso-C. The major respiratory quinone was menaquinone-9(H). The polar lipids of strain CBA3103 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified glycolipids and three unidentified phospholipids. Based on the genotypic, phenotypic and chemotaxonomic analyses, strain CBA3103 represents a novel species of the genus , for which the name sp. nov. (type strain CBA3103=KACC 21446=DSM 110288) is proposed.
Topics: Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Geologic Sediments; Glycolipids; Phospholipids; Phylogeny; Propionibacteriaceae; RNA, Ribosomal, 16S; Republic of Korea; Rivers; Sequence Analysis, DNA; Vitamin K 2
PubMed: 32589569
DOI: 10.1099/ijsem.0.004289 -
Research in Microbiology 2017Progress in next-generation sequencing technologies has facilitated investigations into microbial dynamics. An important bacterium in the dairy industry is...
Progress in next-generation sequencing technologies has facilitated investigations into microbial dynamics. An important bacterium in the dairy industry is Propionibacterium freudenreichii, which is exploited to manufacture Swiss cheeses. A healthy culture of these bacteria ensures a consistent cheese with formed 'eyes' and pleasant flavour profile, and the investigation of prophages and their interactions with these bacteria could assist in the maintenance of the standard of this food product. Two bacteriophages, termed PFR1 and PFR2, were chemically induced using mitomycin C from two different dairy strains of P. freudenreichii. Both phages have identical genomes; however, PFR2 was found to contain an insertion sequence, IS204. Host range characterisation showed that PFR1 was able to form plaques on a wild type Propionibacterium acnes strain, whereas PFR2 could not. The lytic plaques observed on P. acnes were a result of PFR1 inducing the lytic cycle of a pseudolysogenic phage in P. acnes. Further investigation revealed that both PFR1 and PFR2 could infect P. acnes but not replicate. This study demonstrates the dynamic interactions between phages, which may alter their lytic capacity under certain conditions. To our knowledge, this is the first report of two phages interacting to kill their host.
Topics: Bacteriolysis; Cheese; Genome, Viral; Host Specificity; Lysogeny; Microbial Interactions; Mitomycin; Prophages; Propionibacterium; Propionibacterium acnes
PubMed: 27639668
DOI: 10.1016/j.resmic.2016.09.004 -
Cell Host & Microbe Nov 2021Saccharibacteria (TM7) are obligate epibionts living on the surface of their host bacteria and are strongly correlated with dysbiotic microbiomes during periodontitis...
Saccharibacteria (TM7) are obligate epibionts living on the surface of their host bacteria and are strongly correlated with dysbiotic microbiomes during periodontitis and other inflammatory diseases, suggesting they are putative pathogens. However, due to the recalcitrance of TM7 cultivation, causal research to investigate their role in inflammatory diseases is lacking. Here, we isolated multiple TM7 species on their host bacteria from periodontitis patients. These TM7 species reduce inflammation and consequential bone loss by modulating host bacterial pathogenicity in a mouse ligature-induced periodontitis model. Two host bacterial functions involved in collagen binding and utilization of eukaryotic sialic acid are required for inducing bone loss and are altered by TM7 association. This TM7-mediated downregulation of host bacterial pathogenicity is shown for multiple TM7/host bacteria pairs, suggesting that, in contrast to their suspected pathogenic role, TM7 could protect mammalian hosts from inflammatory damage induced by their host bacteria.
Topics: Actinobacteria; Actinomyces; Alveolar Bone Loss; Animals; Bacteria; Bacterial Infections; Bacterial Physiological Phenomena; Collagen; Dental Plaque; Down-Regulation; Genes, Bacterial; Gingivitis; Humans; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Microbiota; N-Acetylneuraminic Acid; Periodontitis; Propionibacteriaceae; Symbiosis; Virulence
PubMed: 34637779
DOI: 10.1016/j.chom.2021.09.009 -
Wei Sheng Wu Xue Bao = Acta... Feb 2017Members of the genus Microlunatus exhibit many potential advantages in managing the environmental pollution caused by phosphorus. The genus was proposed by Nakamura and... (Review)
Review
Members of the genus Microlunatus exhibit many potential advantages in managing the environmental pollution caused by phosphorus. The genus was proposed by Nakamura and co-workers with the name Microlunatus phosphovorus as the type species in 1995. Up to date, the genus Microlunatus encompasses seven validly described species, which were isolated from various environments. Members of the genus Microlunatus share the following genus-specific characteristics, possessing LL-2, 6-diaminopimelic acid in the cell wall peptidoglycan, MK-9(H4) as the predominant menaquinone and diphosphatidylglycerol and phosphatidylglycerol as the phospholipid pattern. Based on the taxonomic results of two newly isolated strains of the genus Microlunatus and the related reference reports, this review summarizes the research advances of the genus Microlunatus, including the genus establishment, taxonomic characteristics, their distribution in the environments, as well as the application prospect in chemical and medical industry.
Topics: Biotechnology; Cell Wall; Environmental Microbiology; Phospholipids; Phylogeny; Propionibacteriaceae
PubMed: 29750480
DOI: No ID Found -
Probiotics and Antimicrobial Proteins Dec 2021In this study, the potential of certain lactic acid bacteria-classified as probiotics and known to be antimicrobially active against pathogens or food-poisoning...
In this study, the potential of certain lactic acid bacteria-classified as probiotics and known to be antimicrobially active against pathogens or food-poisoning microorganisms-was evaluated with respect to their activity against bacterial skin pathogens. The aim of the study was to develop a plaster/bandage for the application of inhibitory substances produced by these probiotics when applied to diseased skin. For this purpose, two Streptococcus salivarius strains and one Lactobacillus plantarum were tested for production of antimicrobials (bacteriocin-like substances) active against Gram-positive and Gram-negative pathogens using established methods. A newly designed membrane test ensured that the probiotics produce antimicrobials diffusible through membranes. Target organisms used were Cutibacterium acnes, Staphylococcus aureus, and Pseudomonas aeruginosa. Moreover, the L. plantarum 8P-A3 strain was tested against additional bacteria involved in skin disorders. The Lactobacillales used were active against all potential skin pathogens tested. These probiotics could be enclosed between polymer membranes-one tight, the other permeable for their products, preserved by vacuum drying, and reactivated after at least three months storage. Importantly, the reactivated pads containing the probiotics demonstrated antibacterial activity on agar plates against all pathogens tested. This suggests that the probiotic containing pads may be topically applied for the treatment of skin disorders without the need for a regular antibiotic treatment or as an adjunctive therapy.
Topics: Bacteriocins; Bandages; Lactobacillus plantarum; Probiotics; Propionibacteriaceae; Pseudomonas aeruginosa; Skin Diseases, Bacterial; Staphylococcus aureus; Streptococcus salivarius
PubMed: 33855669
DOI: 10.1007/s12602-021-09783-7 -
Scientific Reports Jul 2020Our skin provides a physical barrier to separate the internal part of our body from the environment. Maintenance of complex barrier functions is achieved through...
Our skin provides a physical barrier to separate the internal part of our body from the environment. Maintenance of complex barrier functions is achieved through anatomical structures in the skin, the stratified squamous epithelium specialized junctional organelles, called tight junctions (TJs). Several members of our microbial communities are known to affect the differentiation state and function of the colonized organ. Whether and how interactions between skin cells and cutaneous microbes, including Cutibacterium acnes (C. acnes), modify the structure and/or function of our skin is currently only partly understood. Thus, in our studies, we investigated whether C. acnes may affect the epidermal barrier using in vitro model systems. Real-time cellular analysis showed that depending on the keratinocyte differentiation state, the applied C. acnes strains and their dose, the measured impedance values change, together with the expression of selected TJ proteins. These may reflect barrier alterations, which can be partially restored upon antibiotic-antimycotic treatment. Our findings suggest that C. acnes can actively modify the barrier properties of cultured keratinocytes, possibly through alteration of tight cell-to-cell contacts. Similar events may play important roles in our skin, in the maintenance of cutaneous homeostasis.
Topics: Acne Vulgaris; Cell Differentiation; Cells, Cultured; Epidermis; Humans; Keratinocytes; Propionibacteriaceae; Skin Physiological Phenomena; Tight Junction Proteins; Tight Junctions
PubMed: 32733073
DOI: 10.1038/s41598-020-69677-6 -
Carcinogenesis Aug 2020Recent evidence demonstrates the existence of diversified microbiota in the lung. However, the effect of lung carcinogenesis on the flora in lung microenvironment has...
Recent evidence demonstrates the existence of diversified microbiota in the lung. However, the effect of lung carcinogenesis on the flora in lung microenvironment has yet not been well investigated. In this study, we surveyed the microbial composition and diversity in lung tumor and paired adjacent normal tissues obtained from 55 lung cancer patients to test whether any specific tumor-associated microbial features in lung microenvironment can be identified. Compared with non-malignant adjacent tissues, the tumor samples showed significantly lower community richness (α diversity), but no significant difference in overall microbiome dissimilarity (β diversity). Strong intrasubject correlations were observed between tumor sample and its paired non-malignant adjacent tissues. In addition, correlation network analysis found more significant taxa-taxa correlations (adjusted q-value < 0.05) in tumor microenvironment than non-malignant adjacent tissues. At taxa level, we found Propionibacterium genus were significantly reduced in tumor tissues compared with non-malignant adjacent tissues. In summary, the microbiota in tumor tissues showed the lower richness, higher taxa-taxa interaction, and reduction of potential pro-inflammatory microbial genera compared with non-malignant tissues, suggesting the potential link between the tumor microbiota and the altered tumor microenvironment for the further investigation.
Topics: Aged; Carcinogenesis; Female; Humans; Lung Neoplasms; Male; Microbiota; Middle Aged; Propionibacterium; Tumor Microenvironment
PubMed: 32658980
DOI: 10.1093/carcin/bgaa044 -
Antimicrobial Agents and Chemotherapy Feb 2020Antimicrobial-resistant strains have emerged and disseminated throughout the world. The 23S rRNA mutation and (X) gene are known as the major resistance determinants of...
Antimicrobial-resistant strains have emerged and disseminated throughout the world. The 23S rRNA mutation and (X) gene are known as the major resistance determinants of macrolides and clindamycin in We isolated eight high-level macrolide-clindamycin-resistant strains with no known resistance determinants, such as 23S rRNA mutation and (X), from different acne patients in 2008 between 2013 and 2015. The aim of this study was to identify the novel mechanisms of resistance in Whole-genome sequencing revealed the existence of a plasmid DNA, denoted pTZC1 (length, 31,440 bp), carrying the novel macrolide-clindamycin resistance gene (50) and tetracycline resistance gene (W). pTZC1 was detected in all isolates (eight strains) exhibiting high-level macrolide-clindamycin resistance, with no known resistance determinants (MIC of clarithromycin, ≥256 μg/ml; clindamycin, ≥256 μg/ml). Transconjugation experiments demonstrated that the pTZC1 was horizontally transferred among strains and conferred resistance to macrolides, clindamycin, and tetracyclines. Our data showed, for the first time, the existence of a transferable multidrug-resistant plasmid in Increased prevalence of this plasmid will be a great threat to antimicrobial therapy for acne vulgaris.
Topics: Acne Vulgaris; Anti-Bacterial Agents; Bacterial Proteins; Clindamycin; Conjugation, Genetic; Drug Resistance, Multiple, Bacterial; Gene Expression; Gene Transfer, Horizontal; Genome, Bacterial; Humans; Macrolides; Microbial Sensitivity Tests; Phylogeny; Plasmids; Propionibacteriaceae; RNA, Ribosomal, 23S; Tetracycline Resistance; Tetracyclines; Whole Genome Sequencing
PubMed: 31844016
DOI: 10.1128/AAC.01810-19 -
Journal of Bacteriology Jul 2011As part of a larger project to sequence multiple clinical isolates of Propionibacterium acnes, we have produced a draft genome sequence of a novel Propionibacterium...
As part of a larger project to sequence multiple clinical isolates of Propionibacterium acnes, we have produced a draft genome sequence of a novel Propionibacterium species that is closely related to, yet distinct (by sequence) from P. acnes. We have tentatively named this new species Propionibacterium humerusii.
Topics: Actinomycetales Infections; Base Sequence; DNA, Bacterial; Genome, Bacterial; Humans; Molecular Sequence Data; Phylogeny; Propionibacterium
PubMed: 21571999
DOI: 10.1128/JB.05036-11