-
Infection and Immunity May 1985Statistical comparisons of the floras associated with juvenile periodontitis, severe periodontitis, and moderate periodontitis indicated that differences in the... (Comparative Study)
Comparative Study
Statistical comparisons of the floras associated with juvenile periodontitis, severe periodontitis, and moderate periodontitis indicated that differences in the bacterial compositions of affected sites in these populations were not statistically significant. The subgingival flora of affected juvenile periodontitis sites was statistically significantly different from the adjacent supragingival flora and from the subgingival floras of people with healthy gingiva and of children with developing (experimental) gingivitis. However, the subgingival flora of affected juvenile periodontitis sites was not significantly different from the flora of sites with gingival index scores of 1 or 2 in adults with developing (experimental) gingivitis. Of 357 bacterial taxa among over 18,000 isolates, 54 non-treponemal species, 2 treponemal species, and mycoplasma were most associated with diseased periodontal sulci. These species comprised an increasing proportion of the flora during developing gingivitis and constituted over half of the cultivable flora of diseased sites.
Topics: Actinobacillus; Actinomycetales; Adolescent; Adult; Aggressive Periodontitis; Bacteria; Bacteroidaceae; Capnocytophaga; Child; Female; Gingiva; Gingivitis; Humans; Male; Mycoplasma; Periodontal Diseases; Periodontitis; Propionibacteriaceae; Streptococcus; Treponema
PubMed: 3988344
DOI: 10.1128/iai.48.2.507-519.1985 -
Anaerobe Oct 2019Cutibacterium avidum is a gram-positive anaerobic rod belonging to the cutaneous group of human bacteria with preferential colonization of sweat glands in moist areas.... (Review)
Review
Cutibacterium avidum is a gram-positive anaerobic rod belonging to the cutaneous group of human bacteria with preferential colonization of sweat glands in moist areas. The microorganism rarely cause disease, generally delayed prosthetic joint infections (PJIs). We describe the second case of intraperitoneal abscess by C. avidum after an abdominal surgery in an obese female patient and the first case after a non-prosthetic abdominal surgery due to a highly clindamycin resistant strain in a patient with underling conditions. The patient was successfully treated with surgical drainage and beta-lactam antibiotics. Although rare and apparently non-pathogenic, C. avidum may be involved in infections, especially in some high-risk patients with obesity who have undergone surgical incision involving deep folder of the skin. The microorganism was identified by phenotypic methods, MALDI-TOF MS and 16S rRNA gene sequencing. Susceptibility test should be performed in C. avidum because high level resistance to clindamycin could be present. We present a literature review of C. avidum infections.
Topics: Abdominal Abscess; Anti-Bacterial Agents; Clindamycin; DNA, Bacterial; DNA, Ribosomal; Drug Resistance, Bacterial; Gram-Positive Bacterial Infections; Humans; Hysterectomy; Laparotomy; Obesity; Propionibacteriaceae; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 31254654
DOI: 10.1016/j.anaerobe.2019.06.013 -
The Journal of Clinical Investigation Jan 2021TH17 cell subpopulations have been defined that contribute to inflammation and homeostasis, yet the characteristics of TH17 cells that contribute to host defense against...
TH17 cell subpopulations have been defined that contribute to inflammation and homeostasis, yet the characteristics of TH17 cells that contribute to host defense against infection are not clear. To elucidate the antimicrobial machinery of the TH17 subset, we studied the response to Cutibacterium acnes, a skin commensal that is resistant to IL-26, the only known TH17-secreted protein with direct antimicrobial activity. We generated C. acnes-specific antimicrobial TH17 clones (AMTH17) with varying antimicrobial activity against C. acnes, which we correlated by RNA sequencing to the expression of transcripts encoding proteins that contribute to antimicrobial activity. Additionally, we validated that AMTH17-mediated killing of C. acnes and bacterial pathogens was dependent on the secretion of granulysin, granzyme B, perforin, and histone H2B. We found that AMTH17 cells can release fibrous structures composed of DNA decorated with histone H2B that entangle C. acnes that we call T cell extracellular traps (TETs). Within acne lesions, H2B and IL-17 colocalized in CD4+ T cells, in proximity to TETs in the extracellular space composed of DNA decorated with H2B. This study identifies a functionally distinct subpopulation of TH17 cells with an ability to form TETs containing secreted antimicrobial proteins that capture and kill bacteria.
Topics: Acne Vulgaris; Extracellular Traps; Humans; Propionibacteriaceae; RNA-Seq; Skin Diseases, Bacterial; Th17 Cells
PubMed: 33211671
DOI: 10.1172/JCI141594 -
JBJS Case Connector 2020A 32-year-old man developed lumbar discitis and osteomyelitis after receiving a cell-based injection for the treatment of degenerative disc disease. Initial cultures... (Review)
Review
CASE
A 32-year-old man developed lumbar discitis and osteomyelitis after receiving a cell-based injection for the treatment of degenerative disc disease. Initial cultures were negative, but he continued to worsen, and a repeat set of cultures was taken. On day 10, Cutibacterium acnes was isolated. He was then successfully treated with 12 weeks of intravenous antibiotics.
CONCLUSIONS
There is minimal regulation on the preparation or administration of cell-based interventions. It is important to consider slow growing organisms such as C. acnes in patients presenting with spinal infection with insidious onset after these treatments.
Topics: Adult; Anti-Bacterial Agents; Daptomycin; Discitis; Humans; Intervertebral Disc Degeneration; Intervertebral Disc Displacement; Magnetic Resonance Imaging; Male; Osteomyelitis; Propionibacteriaceae; Radionuclide Imaging; Stem Cell Transplantation; Technetium Tc 99m Exametazime; Transplantation, Autologous
PubMed: 32773702
DOI: 10.2106/JBJS.CC.19.00636 -
Biotechnology Journal Feb 2017Traditionally derived from fossil fuels, biological production of propionic acid has recently gained interest. Propionibacterium species produce propionic acid as their...
Traditionally derived from fossil fuels, biological production of propionic acid has recently gained interest. Propionibacterium species produce propionic acid as their main fermentation product. Production of other organic acids reduces propionic acid yield and productivity, pointing to by-products gene-knockout strategies as a logical solution to increase yield. However, removing by-product formation has seen limited success due to our inability to genetically engineer the best producing strains (i.e. Propionibacterium acidipropionici). To overcome this limitation, random mutagenesis continues to be the best path towards improving strains for biological propionic acid production. Recent advances in next generation sequencing opened new avenues to understand improved strains. In this work, we use genome shuffling on two wild type strains to generate a better propionic acid producing strain. Using next generation sequencing, we mapped the genomic changes leading to the improved phenotype. The best strain produced 25% more propionic acid than the wild type strain. Sequencing of the strains showed that genomic changes were restricted to single point mutations and gene duplications in well-conserved regions in the genomes. Such results confirm the involvement of gene conversion in genome shuffling as opposed to long genomic insertions.
Topics: Biotechnology; DNA Shuffling; High-Throughput Nucleotide Sequencing; Propionates; Propionibacterium
PubMed: 27676587
DOI: 10.1002/biot.201600120 -
The British Journal of Dermatology Nov 2018
Topics: Administration, Cutaneous; Propionibacterium; Propionibacterium acnes; Skin
PubMed: 30101491
DOI: 10.1111/bjd.17085 -
MSystems Feb 2023With growing awareness that what we put in and on our bodies affects our health and wellbeing, little is still known about the impact of textiles on the human skin....
With growing awareness that what we put in and on our bodies affects our health and wellbeing, little is still known about the impact of textiles on the human skin. Athletic wear often uses silver threading to improve hygiene, but little is known about its effect on the body's largest organ. In this study, we investigated the impact of such clothing on the skin's chemistry and microbiome. Samples were collected from different body sites of a dozen volunteers over the course of 12 weeks. The changes induced by the antibacterial clothing were specific for individuals, but more so defined by gender and body site. Unexpectedly, the microbial biomass on skin increased in the majority of the volunteers when wearing silver-threaded T-shirts. Although the most abundant taxa remained unaffected, silver caused an increase in diversity and richness of low-abundant bacteria and a decrease in chemical diversity. Both effects were mainly observed for women. The hallmark of the induced changes was an increase in the abundance of various monounsaturated fatty acids (MUFAs), especially in the upper back. Several microbe-metabolite associations were uncovered, including , detected in the upper back area, which was correlated with the distribution of MUFAs, and spp. found in the underarms, which were associated with a series of different bile acids. Overall, these findings point to a notable impact of the silver-threaded material on the skin microbiome and chemistry. We observed that relatively subtle changes in the microbiome result in pronounced shifts in molecular composition. The impact of silver-threaded material on human skin chemistry and microbiome is largely unknown. Although the most abundant taxa remained unaffected, silver caused an increase in diversity and richness of low-abundant bacteria and a decrease in chemical diversity. The major change was an increase in the abundance of various monounsaturated fatty acids that were also correlated with . Additionally, spp., found in the underarms, were associated with different bile acids in the armpit samples. Overall, the impact of the silver-threaded clothing was gender and body site specific.
Topics: Humans; Female; Silver; Clothing; Skin; Textiles; Microbiota; Bacteria; Propionibacteriaceae
PubMed: 36722970
DOI: 10.1128/msystems.00922-22 -
Applied and Environmental Microbiology Jan 1978Propionibacterium acnes, P. avidum, and P. granulosum were quantitatively measured in 50 young adults. The scalp, forehead, external auditory canal, alae nasi, anterior...
Propionibacterium acnes, P. avidum, and P. granulosum were quantitatively measured in 50 young adults. The scalp, forehead, external auditory canal, alae nasi, anterior nares, groin, rectum, and antecubital and popliteal fossa were sampled. These represent various cutaneous microenvironments, differing in moisture, density of sweat, sebaceous glands, and extent of anaerobiosis. These studies show that the propionibacteria are ubiquitous on the skin, with P. acnes predominant in both prevalence and population, especially in areas rich in sebum. P. granulosum recovery paralled that of P. acnes, but the density was significantly lower. P. avidum was found mainly in moist areas and the retum, suggesting an intestinal reservoir.
Topics: Adolescent; Adult; Humans; Male; Propionibacterium; Propionibacterium acnes; Sebum; Skin; Species Specificity; Water
PubMed: 623473
DOI: 10.1128/aem.35.1.62-66.1978 -
Biochimica Et Biophysica Acta Aug 1962
Topics: Fumarate Hydratase; Propionibacterium
PubMed: 13863515
DOI: 10.1016/0006-3002(62)90279-2 -
Bioresource Technology Oct 2022In the present study, a biochemical strategy for improving propionic acid production from kitchen waste acidification by bioaugmentation with Propionibacterium...
In the present study, a biochemical strategy for improving propionic acid production from kitchen waste acidification by bioaugmentation with Propionibacterium acidipropionici (P. acidipropionici) was investigated. When the inoculum of P. acidipropionici was 30% (w/w) of the seeding sludge, the propionic acid production increased by 79.57%. Further, bioaugmentation improved the relative abundance of Firmicute and Actinobacteria. The results of metagenomic analysis further reveal that the ATP-binding cassette (ABC) transporters and all related pathways of Propanoate metabolism (ko00640) were enriched when P. acidipropionici was added. For Propanoate metabolism, most functional genes involved in the conversion from Glycolysis / Gluconeogenesis (ko00010) to Propanoyl-CoA and conversion from Propanoyl-CoA to propionic acid were enhanced after bioaugmentation with P. acidipropionici, thereby promoting propionic acid production. As such, bioaugmentation with P. acidipropionici was effective in the anaerobic acidification of kitchen waste for propionic acid production.
Topics: Anaerobiosis; Coenzyme A; Hydrogen-Ion Concentration; Metagenomics; Propionates; Propionibacteriaceae; Propionibacterium
PubMed: 36031136
DOI: 10.1016/j.biortech.2022.127843