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Canadian Journal of Microbiology Nov 2022Iron is essential for almost all bacteria, and iron homeostasis is precisely controlled by the ferric uptake regulator (Fur). The Fur regulons have been well...
Iron is essential for almost all bacteria, and iron homeostasis is precisely controlled by the ferric uptake regulator (Fur). The Fur regulons have been well characterized in some model bacteria, yet little is known in the common opportunistic pathogen . In this study, Fur regulon and iron-responsive genes in were mainly defined by in silico and proteomic analyses. The results showed that about 250 potential Fur-regulated operons including 14 transcriptional factors were predicted, while 559 proteins exhibited differential expression in response to iron deficiency, not all being directly regulated by Fur, such as transcriptional factors , and . Collectively, these results demonstrated that Fur functioned as a global regulatory protein to repress or activate expression of a large repertoire of genes in ; besides, not all the iron-responsive genes were directly regulated by Fur, whereas indirectly regulated through other mechanisms such as additional transcriptional regulatory proteins.
Topics: Gene Expression Regulation, Bacterial; Iron; Proteus vulgaris; Repressor Proteins; Bacterial Proteins; Proteomics; Regulon; Transcription Factors
PubMed: 36214343
DOI: 10.1139/cjm-2021-0310 -
American Journal of Diseases of... Apr 1949
Topics: Bacteria; Humans; Meningitis; Proteus vulgaris
PubMed: 18119697
DOI: 10.1001/archpedi.1949.02030040466004 -
Journal of Biosciences 2021is a rod-shaped Gram-negative bacterium known to be the member of Enterobacteriaceae that is able to cause disease in human being. Generally, non-protein-coding RNAs...
is a rod-shaped Gram-negative bacterium known to be the member of Enterobacteriaceae that is able to cause disease in human being. Generally, non-protein-coding RNAs (npcRNAs) do not code for proteins, but they play a vital role in gene regulation at the RNA level including pathogenicity. The present study aims at elucidating homologous npcRNAs from other bacteria in . A comparative genomic analysis was carried out to identify npcRNA homolog of other Enterobacteriaceae pathogens in . A total of 231 npcRNAs previously reported in and were screened using BLASTn tool against genome. Interestingly, 33 npcRNAs are homologs to . Northern blot analysis of 6 out of 33 npcRNA candidates confirmed their expression and showed that most of them are differentially expressed during lag, exponential and stationary growth phases. This study is the first approach of identification and characterization of npcRNAs in . Hence, this could be a pioneer study to further validate the regulatory functions of these npcRNAs to fill the gaps in understanding of the pathogenicity of .
Topics: Genomics; Humans; Proteus vulgaris; RNA, Untranslated
PubMed: 34845992
DOI: No ID Found -
Microbial Drug Resistance (Larchmont,... Oct 2021is an important foodborne opportunistic pathogen, both environmentally and clinically. The use of appropriate antibiotics has significant therapeutic effects, but has...
is an important foodborne opportunistic pathogen, both environmentally and clinically. The use of appropriate antibiotics has significant therapeutic effects, but has led to the emergence and spread of drug-resistant strains. In this study, a strain, designated "P3M," was isolated from in Tianjin, China. The whole genome of P3M was sequenced, generating detailed information, including the key genes involved in important metabolic pathways and their physiological functions. A total of 218 antibiotic resistance genes (ARGs) were predicted in the genome. The determination of various minimum inhibitory concentrations indicated that P3M is a multidrug-resistant (MDR) bacterium, with significant resistance to 16 antibiotics in seven categories. Determination of fractional inhibitory concentration index showed that the combination of ciprofloxacin plus tetracycline exhibited synergistic antimicrobial activity. Bioinformatics and phylogenetic analyses detected the presence of two two-component systems that mediate multidrug resistance and several mobile genetic elements involved in the horizontal transfer of ARGs in P3M. strains represent a serious challenge to clinicians and infection control teams for its ubiquity worldwide and close relevance with human life. To the best of our knowledge, we report the first isolation and characterization of an important foodborne MDR strain, and this study will provide necessary theoretical basis for the selection and clinical use of the appropriate antibiotics.
Topics: Animals; Anti-Bacterial Agents; China; Drug Resistance, Multiple, Bacterial; Foodborne Diseases; Genes, Bacterial; Microbial Sensitivity Tests; Penaeidae; Proteus vulgaris
PubMed: 33877915
DOI: 10.1089/mdr.2020.0502 -
Genomics Jan 2022Proteus phage vB_PvuS_Pm34 (Pm34) isolated from the sewage, is a novel virus specific to Proteus vulgaris. Pm34 belonged to the family Siphovirodae with an icosahedron...
Proteus phage vB_PvuS_Pm34 (Pm34) isolated from the sewage, is a novel virus specific to Proteus vulgaris. Pm34 belonged to the family Siphovirodae with an icosahedron capsid head and a non-contractile tail. Its genome was 39,558 bp in length with a G + C content of 41.4%. Similarity analysis showed that Pm34 shared low identities of 27.6%-38.4% with any other Proteus phages, but had the 96% high identity with Proteus mirabilis AOUC-001. In the genome of Pm34, 70 open reading frames was deduced and 32 had putative functions including integrase and host lysis proteins. No tRNAs, antibiotic resistance and virulence genes were detected. Pm 34 presented a broad pH (4-8) and good temperature tolerance (<40 °C). This is the first report of the bacteriophage specific to P. vulgaris, which can enrich the knowledge of bacteriophages of Prouteus bacteria and provide the possibility for the alternative treatment of P. vulgaris infection.
Topics: Bacteriophages; Genome, Viral; Genomics; Open Reading Frames; Proteus mirabilis; Proteus vulgaris; Siphoviridae
PubMed: 34839020
DOI: 10.1016/j.ygeno.2021.11.033 -
Polish Journal of Microbiology Sep 2020species are common opportunistic bacteria and foodborne pathogens. The proper detection of can effectively reduce the occurrence of food-borne public health events....
species are common opportunistic bacteria and foodborne pathogens. The proper detection of can effectively reduce the occurrence of food-borne public health events. and are the two most important pathogens in the genus. In this study, a dual TaqMan Real-Time PCR method was established to simultaneously detect and distinguish and in samples. The method exhibited good specificity, stability, and sensitivity. Specifically, the minimum detection concentrations of and in pure bacterial cultures were 6.08 × 10 colony forming units (CFU)/ml and 4.46 × 10 CFU/ml, respectively. Additionally, the minimum detectable number of and in meat and milk was 10 CFU/g. In addition, the method can be used to distinguish between strains of and within two hours. Overall, it is a sensitive, easy-to-use, and practical test for the identification and classification of in food.
Topics: Animals; DNA, Bacterial; Food Microbiology; Foodborne Diseases; Genes, Bacterial; Limit of Detection; Milk; Pork Meat; Proteus mirabilis; Proteus vulgaris; Real-Time Polymerase Chain Reaction; Reproducibility of Results
PubMed: 33574858
DOI: 10.33073/pjm-2020-032 -
Journal of Infection and Chemotherapy :... Jun 2023A hemin-requiring Proteus vulgaris small-colony variant (SCV) was isolated from a urine culture. This isolate was grown on 5% sheep blood agar but not on modified...
A hemin-requiring Proteus vulgaris small-colony variant (SCV) was isolated from a urine culture. This isolate was grown on 5% sheep blood agar but not on modified Drigalski agar. The single nucleotide substitution was found in the SCV of the hemC gene (c.55C > T), and this substitution caused a nonsense mutation (p.Gln19Ter). Porphyrin test results showed that the biosynthesis of δ-aminolevulinic acid stopped up to porphobilinogen and not pre-uroporphyrinogen due to a mutation in the hemC gene. To our knowledge, this is the first report of hemin-requiring P. vulgaris.
Topics: Animals; Sheep; Hemin; Proteus vulgaris; Agar; Porphyrins; Culture Media
PubMed: 36996937
DOI: 10.1016/j.jiac.2023.03.015 -
Bioelectrochemistry (Amsterdam,... Aug 2018One of the most challenging problems when trying to recycle urine for different purposes is the removal of urea. In this project we studied an ureolysis system using the...
One of the most challenging problems when trying to recycle urine for different purposes is the removal of urea. In this project we studied an ureolysis system using the bacterium Proteus vulgaris for the transformation of urea to ammonia and its subsequent oxidation to nitrogen at a Pt working electrode. Our system was tested under different pH, microbial reaction times, and urea and bacteria concentrations. Our results indicate that a pH8 is optimal for the combined Proteus vulgaris urease activity and the ammonia oxidation reaction at a Pt electrode. The reaction time and concentration dependence on the ammonia oxidation reaction current densities was also studied. Results showed limited ammonia oxidation under high urea concentrations in ~2.5×10cfu/mL Proteus vulgaris in synthetic urine.
Topics: Ammonia; Biotransformation; Electrochemical Techniques; Electrodes; Hydrogen-Ion Concentration; Nitrogen; Oxidation-Reduction; Platinum; Proteus vulgaris; Urea
PubMed: 29679910
DOI: 10.1016/j.bioelechem.2018.03.017 -
International Journal of Systematic and... Sep 2000Strains traditionally identified as Proteus vulgaris formed three biogroups. Biogroup 1, characterized by negative reactions for indole production, salicin fermentation...
Strains traditionally identified as Proteus vulgaris formed three biogroups. Biogroup 1, characterized by negative reactions for indole production, salicin fermentation and aesculin hydrolysis, is now known as Proteus penneri. Biogroup 2, characterized by positive reactions for indole, salicin and aesculin, was shown by DNA hybridization (hydroxyapatite method) to be a genetic species separate from biogroup 1 and from biogroup 3 which is positive for indole production and negative for salicin and aesculin. In this study, 52 strains were examined, of which 36 strains were Proteus vulgaris biogroup 3, which included the current type strain of the species P. vulgaris (ATCC 29905T), and compared to seven strains of Proteus vulgaris biogroup 2 and nine type strains of other species in the genera Proteus, Providencia and Morganella. By DNA hybridization, these 36 strains were separated into four distinct groups, designated as Proteus genomospecies 3, 4, 5 and 6. DNAs within each separate Proteus genomospecies were 74-99% related to each other in 60 degrees C hybridization reactions with < or = 4.5% divergence between related sequences. Proteus genomospecies 3 contained the former P. vulgaris type strain and one other strain and was negative in reactions for salicin fermentation, aesculin hydrolysis and deoxyribonuclease, unlike the reactions associated with strains considered as typical P. vulgaris which are positive in reactions for salicin, aesculin and DNase. Genomospecies 3 can be distinguished from Proteus genomospecies 4, 5 and 6 because it is negative for Jordan's tartrate. Proteus genomospecies 4, containing five strains, was differentiated from Proteus penneri, genomospecies 3 and 6 and most, but not all, strains of genomospecies 5, by its ability to ferment L-rhamnose. Proteus genomospecies 5 and 6, containing 18 and 11 strains, respectively, could not be separated from each other by traditional biochemical tests, by carbon source utilization tests or SDS-PAGE of whole-cell proteins. In an earlier publication, a request was made to the Judicial Commission that the former type strain of P. vulgaris (ATCC 13315) be replaced by P. vulgaris biogroup 2 strain ATCC 29905T, a strain considered more biochemically typical of P. vulgaris strains. This would have the effect of assigning the name P. vulgaris to P. vulgaris biogroup 2. Since this request has been acceded to, the name Proteus hauseri is herein proposed for Proteus vulgaris genomospecies 3. Its type strain is ATCC 700826T. Proteus genomospecies 4, 5 and 6 will remain unnamed until better phenotypic differentiation can be accomplished. All Proteus genomospecies were similar in their antimicrobial susceptibility patterns. Nineteen strains were isolated from urine, four from faeces, two from wounds, nine from other human sources and two from animals.
Topics: Anti-Bacterial Agents; Bacterial Proteins; Bacterial Typing Techniques; DNA, Bacterial; Electrophoresis; Genome, Bacterial; Humans; Microbial Sensitivity Tests; Nucleic Acid Hybridization; Nucleic Acids; Phenotype; Proteus; Proteus Infections; Proteus vulgaris
PubMed: 11034498
DOI: 10.1099/00207713-50-5-1869 -
Archivum Immunologiae Et Therapiae... 2007Proteus rods are currently subdivided into five named species, i.e. Proteus mirabilis, P. vulgaris, P. penneri, P. hauseri, and P. myxofaciens, and three unnamed Proteus...
INTRODUCTION
Proteus rods are currently subdivided into five named species, i.e. Proteus mirabilis, P. vulgaris, P. penneri, P. hauseri, and P. myxofaciens, and three unnamed Proteus genomospecies 4 to 6. Based on the serospecificity of the lipopolysaccharide (LPS; O-antigen), strains of P. mirabilis and P. vulgaris were divided into 49 O-serogroups and 11 additional O-serogroups were proposed later. About 15 further O-serogroups have been proposed for the third medically important species, P. penneri. Here the serological classification of P. vulgaris strain TG 251, which does not belong to these serogroups, is reported. Serological investigations also allowed characterization of the epitope specificity of its LPS.
MATERIALS AND METHODS
Purified LPSs from five Proteus strains were used as antigens in enzyme immunosorbent assay (EIA), SDS/PAGE, and Western blot and alkali-treated LPSs in the passive immunohemolysis (PIH) test, inhibition of PIH and EIA, and absorption of the rabbit polyclonal O-antisera with the respective LPS.
RESULTS
The serological studies of P. vulgaris TG 251 LPS indicated the identity of its O-polysaccharide with that of P. penneri O65. The antibody specificities of P. vulgaris TG 251 and P. penneri O65 O-antisera, were described.
CONCLUSIONS
P. vulgaris TG 251 was classified to the Proteus O65 serogroup. Two disaccharide-associated epitopes present in P. vulgaris TG 251 and P. penneri O65 LPSs are suggested to be responsible for cross-reactions with three heterologous Proteus strains.
Topics: Animals; Antigens, Bacterial; Cross Reactions; Epitopes; Lipopolysaccharides; O Antigens; Proteus penneri; Proteus vulgaris; Serotyping
PubMed: 17557147
DOI: 10.1007/s00005-007-0020-z