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The American Journal of Tropical... Aug 2019is a member of the family Enterobacteriaceae that has been commonly implicated as a causative agent of diarrheal infection in humans and animals. Recent outbreaks of... (Review)
Review
is a member of the family Enterobacteriaceae that has been commonly implicated as a causative agent of diarrheal infection in humans and animals. Recent outbreaks of in both developing and developed countries have raised public health concerns. Several studies have suggested that can cause diarrhea by invading the intestinal mucosa, although its pathogenicity has not been well established. Often routine laboratory investigations that seek etiological agents of diarrhea do not actively pursue detection. Therefore, routine laboratory diagnosis should be given more attention for better understanding the epidemiology and pathogenicity of .
Topics: Animals; Diarrhea; Disease Outbreaks; Enterobacteriaceae Infections; Feces; Foodborne Diseases; Providencia
PubMed: 31218997
DOI: 10.4269/ajtmh.18-0376 -
Emerging Microbes & Infections Dec 2023as an opportunistic pathogen can cause serious infection, and moreover the emergence of multi-drug-resistant strains poses a potentially life-threatening risk to...
as an opportunistic pathogen can cause serious infection, and moreover the emergence of multi-drug-resistant strains poses a potentially life-threatening risk to public health. However, a comprehensive genomic study to reveal the population structure and dissemination of is still lacking. In this study, we conducted a genomic epidemiology analysis on the 580 global sequenced isolates, including 257 ones sequenced in this study (42 ones were fully sequenced). We established a genome sequence-based species classification scheme for , redefining the conventional 11 species into seven genocomplexes that were further divided into 18 genospecies, providing an extensively updated reference for species discrimination based on the largest genome dataset to date. We then dissected the profile of antimicrobial resistance genes and the prevalence of multi-drug-resistant strains among these genocomplexes/genospecies, disclosing the presence of diverse and abundant antimicrobial resistance genes and high resistance ratios against multiple classes of drugs in . We further dissected the genetic basis for the spread of in . genes were mainly carried by five incompatible (Inc) groups of plasmids: IncC, IncW, Inc, Inc, and Inc, and the last three were newly designated in this study. By tracking the spread of -carrying plasmids, IncC, Inc, Inc, and Inc plasmids were found to be highly involved in parallel horizontal transfer or vertical clonal expansion of among . Overall, our study provided a comprehensive genomic view of species differentiation, antimicrobial resistance prevalence, and plasmid-mediated dissemination in .
Topics: Providencia; Anti-Bacterial Agents; Plasmids; beta-Lactamases; Genomics; Microbial Sensitivity Tests
PubMed: 37874004
DOI: 10.1080/22221751.2023.2275596 -
The Journal of Antimicrobial... Mar 1984
Review
Topics: Aminoglycosides; Anti-Bacterial Agents; Drug Resistance, Microbial; Humans; Lactams; Plasmids; Proteus; Proteus Infections; Providencia
PubMed: 6373713
DOI: 10.1093/jac/13.3.209 -
International Journal of Systematic and... Sep 2019Strain WCHPr000369 was recovered from a human rectal swab in China in 2015. Phylogenetic analysis based on its 16S rRNA gene suggested that the strain belonged to the...
Strain WCHPr000369 was recovered from a human rectal swab in China in 2015. Phylogenetic analysis based on its 16S rRNA gene suggested that the strain belonged to the genus . The genome sequence of the strain had a 77.30-90.43% average nucleotide identity (ANI) and 20.9-41.5 % DNA-DNA hybridization (DDH) score with those of type strains of known species. The ANI and DDH values indicated that the strain was likely to belong to a new species. Multi-locus sequence analysis on the , , , and housekeeping genes also revealed that the strain was distinct from any previously described species of the genus . Strain WCHPr000369 could be distinguished from all known species by the combination of positive urease reaction and the ability to utilize citrate. Genotypic and phenotypic characteristics from this study indicated that strain WCHPr000369 should be considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WCHPr000369 (=GDMCC1.1382=KCTC 62577).
Topics: Aged; Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Female; Genes, Bacterial; Humans; Nucleic Acid Hybridization; Phylogeny; Providencia; RNA, Ribosomal, 16S; Rectum; Sequence Analysis, DNA
PubMed: 31162027
DOI: 10.1099/ijsem.0.003502 -
Reviews of Infectious Diseases 1986The long-term-catheterized urinary tract may offer a particular niche to Providencia stuartii, which is otherwise an uncommon clinical isolate. Published accounts of... (Review)
Review
The long-term-catheterized urinary tract may offer a particular niche to Providencia stuartii, which is otherwise an uncommon clinical isolate. Published accounts of bacteriuria in patients catheterized for long periods indicate that P. stuartii has often been found as frequently as familiar uropathogens such as Escherichia coli, Proteus mirabilis, enterococcus, and Pseudomonas aeruginosa. As in most nosocomial infections, the frequency of isolation of a given species has commonly differed among institutions. In the future P. stuartii may be more frequently encountered as a nosocomial pathogen in nursing homes and in acute care hospitals to which bacteriuric patients are transferred. This trend appears likely because of the increasingly large nursing-home population, the predilection of the bacterium for the long-term-catheterized urinary tract, the opportunity for nosocomial transmission from this reservoir, the resistance of the organism to multiple antibiotics, and the occasional systemic illness and bacteremia caused by P. stuartii.
Topics: Adult; Aged; Bacteriuria; Catheters, Indwelling; Escherichia coli; Humans; Middle Aged; Proteus; Proteus Infections; Proteus mirabilis; Providencia; Pseudomonas aeruginosa; Time Factors; Urinary Tract Infections
PubMed: 3081988
DOI: 10.1093/clinids/8.1.61 -
BMC Genomic Data Sep 2023Providencia is a genus of gram-negative bacteria within the order Enterobacterales, closely related to Proteus and Morganella. While ubiquitous in the environment, some...
OBJECTIVES
Providencia is a genus of gram-negative bacteria within the order Enterobacterales, closely related to Proteus and Morganella. While ubiquitous in the environment, some species of Providencia, such as P. rettgeri and P. stuartii, are considered emerging nosocomial pathogens and have been implicated in urinary tract infection, gastrointestinal illness, and travelers' diarrhea. Given their intrinsic resistance to many commonly used antibiotics, this study aimed to isolate and sequence bacteriophages targeting a clinical P. rettgeri isolate.
DATA DESCRIPTION
Here we report the complete genome sequence of three novel Providencia phages, PibeRecoleta, Stilesk and PatoteraRojo, which were isolated against a clinical P. rettgeri strain sourced from a patient in a metropolitan hospital in Victoria, Australia. The three phages contain dsDNA genomes between 60.7 and 60.9 kb in size and are predicted to encode between 72 and 73 proteins. These three new phages, which share high genomic similarity to two other Providencia phages previously isolated on P. stuartii, serve as important resources in our understanding about Providencia bacteriophages and the potential for future phage-based biotherapies.
Topics: Humans; Diarrhea; Providencia; Travel; Bacteriophages; Dysentery; Hospitals, Urban; Victoria
PubMed: 37658299
DOI: 10.1186/s12863-023-01153-2 -
International Journal of Systematic and... Aug 2022A facultatively anaerobic, Gram-negative, rod-shaped bacterial strain designated as LLDRA6, was isolated from heavy metal contaminated soils collected near a ceased...
A facultatively anaerobic, Gram-negative, rod-shaped bacterial strain designated as LLDRA6, was isolated from heavy metal contaminated soils collected near a ceased smelting factory at Zhuzhou, Hunan Province, China. Strain LLDRA6 has the ability to oxidize Mn(II) and generate biogenic manganese oxides. The strain can grow in a wide range of temperature from 10-42°C and pH from 5 to 10. Comparative analysis of its complete 16S rRNA gene sequence suggests that strain LLDRA6 is highly similar to species within the genus . The complete genome of LLDRA6 is 4 342 370 bp with 40.18 mol% of G+C content and contains no plasmids. In comparison to the genomes of type strains in , LLDRA6 shows average nucleotide identity values between 76.60 and 80.89 %, and digital DNA-DNA hybridization values in a range of 21.2-24.6 %. Both multilocus sequence analysis and genomic phylogenetics indicate a new taxonomic status for LLDRA6 in . Chemotaxonomic analyses for LLDRA6 show that the predominant cellular fatty acids are C, C and cyclo-C, accounting for 32.7, 16.1 and 10.3 % of total fatty acids, respectively. The polar lipids consist of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, four unidentified aminolipids, one unidentified phospholipid and three unidentified lipids. Within the cell wall, ribose and -diaminopimelic acid are the characteristic constituents for saccharides and amino acids, respectively. Respiratory quinones on cell membranes are composed of menaquinone (MK) and ubiquinone (coenzyme Q), including MK-8 (100.0 %), Q-7 (13.7 %) and Q-8 (86.3 %). Moreover, the positive results from d-lyxose and d-mannitol fermentation tests indicate that LLDRA6 is totally different from all the type strains within the genus . In summary, strain LLDRA6 represents a novel species in the genus , for which the name sp. nov. (type strain LLDRA6=CCTCC AB 2021154=KCTC 92091) is proposed.
Topics: Bacteria; Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Fatty Acids; Metals, Heavy; Oxidation-Reduction; Phospholipids; Phylogeny; Providencia; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil; Soil Microbiology
PubMed: 35930465
DOI: 10.1099/ijsem.0.005474 -
Biochemistry. Biokhimiia Jul 2013The genus Providencia consists of eight species of opportunistic pathogenic enterobacteria that can cause enteric diseases and urinary tract infections. The existing... (Review)
Review
The genus Providencia consists of eight species of opportunistic pathogenic enterobacteria that can cause enteric diseases and urinary tract infections. The existing combined serological classification scheme of three species, P. alcalifaciens, P. stuartii, and P. rustigianii, is based on the specificity of O-antigens (O-polysaccharides) and comprises 63 O-serogroups. Differences between serogroups are related to polymorphism at a specific genome locus, the O-antigen gene cluster, responsible for O-antigen biosynthesis. This review presents data on structures of 36 O-antigens of Providencia, many of which contain unusual monosaccharides and non-carbohydrate components. The structural data correlate with the immunospecificity of the O-antigens and enable substantiation on a molecular level of serological relationships within the genus Providencia and between strains of Providencia and bacteria of the genera Proteus, Escherichia, and Salmonella. Peculiar features of the O-antigen gene cluster organization in 10 Providencia serogroups and biosynthetic pathways of nucleotide precursors of specific monosaccharide components of the O-antigens also are discussed.
Topics: Carbohydrate Sequence; Escherichia; Genome, Bacterial; Lipopolysaccharides; Molecular Sequence Data; Monosaccharides; Multigene Family; O Antigens; Providencia; Salmonella
PubMed: 24010842
DOI: 10.1134/S0006297913070110 -
Diagnostic Microbiology and Infectious... Dec 2023A member of the Enterobacteriaceae family, Providencia alcalifaciens is typically recognized as a source of gastrointestinal illness. Although its pathogenicity is not...
A member of the Enterobacteriaceae family, Providencia alcalifaciens is typically recognized as a source of gastrointestinal illness. Although its pathogenicity is not well known, many studies have suggested its mechanism of action involves the invasion of the intestinal mucosal layer. Although P. alcalifaciens is a urease producing microorganism, it has not been associated with the formation of a staghorn calculus in the setting of a urinary tract infection. This organism is neither commonly pursued in research or investigation nor is it commonly tested for in the clinical setting. This is especially true when combined with other disease processes, such as calculus formation. The advancement of antibiotic resistance, such as carbapenemase-producing strains, should bring more attention and routine investigation to this organism in the acute stage of infection. In this case report we introduce a 43-year-old Cuban female, who presents with a left-sided staghorn calculi and urine culture positive for carbapenemase-producing P. alcalifaciens.
Topics: Humans; Female; Adult; Staghorn Calculi; Providencia; Enterobacteriaceae
PubMed: 37716218
DOI: 10.1016/j.diagmicrobio.2023.116055 -
Current Microbiology Dec 2021Despites Providencia heimbachae has been isolated from human, penguin, and bovine fetus, relatively little information is available regarding the pathogenicity and...
Despites Providencia heimbachae has been isolated from human, penguin, and bovine fetus, relatively little information is available regarding the pathogenicity and biologic characteristics of P. heimbachae. Here, we report that investigation of post-weaning diarrhea yielded bacterial isolates identified as P. heimbachae based on the biochemical tests and 16S ribosomal DNA sequence analysis. The two isolates were positive for utilization of Malonate, no gas production from glucose, and non-fermentation of D-mannitol, D-Galactose, and L-Rhamnose that were different from those of the type strain, and both of them have the ability of adhesion and invasion to IPEC-J2 cells, and were resistant to 21 out of the 41 antibiotics tested. In addition, the isolate 99101 was highly pathogenic to mice and piglets. Histopathology studies on nerve tissue of piglets that developed hindlimb paralysis showed microglia cell infiltration and neuron damage in the spinal cord. Notably, the strains could grow under low temperature (4 °C), which raise attention of a new risk factor for food safety. To the best of our knowledge, this is the first report of P. heimbachae strain caused post-weaning diarrhea in piglets in both natural and experimental conditions. These findings extended the knowledge of P. heimbachae as an important zoonotic agent, which should be given more attention during surveillance and diagnostics.
Topics: Animals; Cattle; Diarrhea; Mice; Phenotype; Providencia; Swine; Swine Diseases; Weaning
PubMed: 34878563
DOI: 10.1007/s00284-021-02697-1