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The Journal of Hospital Infection Jun 1985A selective differential medium has been used to search for Providencia stuartii in sewage, sewage contaminated natural waters and the faeces and skin of a small... (Comparative Study)
Comparative Study
A selective differential medium has been used to search for Providencia stuartii in sewage, sewage contaminated natural waters and the faeces and skin of a small population of healthy non-hospitalized males. Colonization of 12 male patients with long-term indwelling bladder catheters and the general environment of the spinal injury unit was also examined. Providencia stuartii was not isolated from the non-hospital samples, but colonization of the urine (two patients) faeces (five patients) and skin (eight patients) was observed. Apart from equipment that had been in contact with patients urine or skin there was no general contamination of the ward environment suggesting that colonized patients were the main reservoir of this multiply antibiotic-resistant nosocomial pathogen.
Topics: Bacteriuria; Catheters, Indwelling; Environmental Microbiology; Feces; Groin; Humans; Male; Proteus; Providencia; Sewage; Skin; Spinal Injuries; Urinary Bladder; Urinary Catheterization; Wales; Water Microbiology
PubMed: 2862199
DOI: No ID Found -
Nihon Rinsho. Japanese Journal of... Jun 2002Providencia alcalifaciens, a member of the family Enterobacteriaceae, has been thought to be a normal flora or an opportunistic pathogen. In 1996, a large outbreak... (Review)
Review
Providencia alcalifaciens, a member of the family Enterobacteriaceae, has been thought to be a normal flora or an opportunistic pathogen. In 1996, a large outbreak (including 270 patients) of acute gastroenteritis occurred in Fukui. No known enteropathogens were detected in fecal samples of the patients, but P. alcalifaciens was detected from 7 of the 18 samples. The isolates were a clonal by pulsed-field gel electrophoresis. The specific antibody against the isolated strain was elevated in the patients' sera. The isolates showed invasiveness into Caco-2 cells and fluid-accumulation in rabbit ileal loops. These results indicate that P. alcalifaciens can be one of the causative agents of gastroenteritis or food-poisoning.
Topics: Acute Disease; Animals; Disease Outbreaks; Electrophoresis, Gel, Pulsed-Field; Foodborne Diseases; Gastroenteritis; Humans; Japan; Providencia; Rabbits
PubMed: 12078099
DOI: No ID Found -
Japanese Journal of Infectious Diseases 2012In the present study, we examined the prevalence of Providencia spp. strains among children with diarrhea in Japan. We developed a Providencia genus-specific polymerase...
In the present study, we examined the prevalence of Providencia spp. strains among children with diarrhea in Japan. We developed a Providencia genus-specific polymerase chain reaction (PCR) method, and the specificity and sensitivity was evaluated to be 100% with various bacterial strains including 7 genera and 13 species. Five of 345 samples (1.4%) were positive by PCR using a Providencia genus-specific primer targeting the 16S rRNA gene. The single species Providencia rettgeri was isolated from 4 stool samples of children with diarrhea. The prevalence of Providencia spp. in children with diarrhea in Japan is lower than that previously reported for Japanese travelers abroad with diarrhea.
Topics: Bacteriological Techniques; Child; Child, Preschool; DNA Primers; DNA, Ribosomal; Diarrhea; Enterobacteriaceae Infections; Feces; Female; Humans; Infant; Infant, Newborn; Japan; Male; Molecular Diagnostic Techniques; Polymerase Chain Reaction; Prevalence; Providencia; RNA, Ribosomal, 16S; Sensitivity and Specificity
PubMed: 23183210
DOI: 10.7883/yoken.65.545 -
Current Microbiology Mar 2014Investigation of a serious pig disease with high mortality and typical lung lesions yielded a bacterial isolate identified as Providencia alcalifaciens based on the 16S...
Investigation of a serious pig disease with high mortality and typical lung lesions yielded a bacterial isolate identified as Providencia alcalifaciens based on the 16S ribosomal DNA sequence analysis. The pathogenicity of this bacterial isolate was confirmed in piglets and mice. The bacterial strain caused the typical illness in piglets, which suffered serious dyspnea and hemorrhagic pneumonia. The drug resistance spectrum of the bacterium was also determined. The results indicated that the isolate is resistant to 12 antibiotics and intermediately resistant to 10 antibiotics out of the 34 antibiotics tested. The current study is the first to report a serious lung disease in piglets caused by a multidrug resistant P. alcalifaciens isolate, which should be given more attention during surveillance and diagnostics.
Topics: Animals; Anti-Bacterial Agents; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae Infections; Histocytochemistry; Lung; Mice; Microscopy; Molecular Sequence Data; Phylogeny; Pneumonia, Bacterial; Providencia; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Survival Analysis; Swine; Swine Diseases
PubMed: 24129837
DOI: 10.1007/s00284-013-0470-y -
Enfermedades Infecciosas Y... 2017In Colombia, between 2012 and 2013, 19 isolates with NDM were identified, of which 14 corresponded to Providencia rettgeri.
INTRODUCTION
In Colombia, between 2012 and 2013, 19 isolates with NDM were identified, of which 14 corresponded to Providencia rettgeri.
METHODS
The isolates were identified by Vitek-2, and antimicrobial susceptibility was evaluated by broth microdilution. The carbapenemase phenotypes were determined with Modified Hodge Test and synergy tests with EDTA/SMA and APB, the genotypes by PCR using specific primers for KPC, GES, IMP, VIM, OXA-48 and NDM, and genetic relationships were established with DiversiLab.
RESULTS
The isolates were resistant to carbapenems, third-generation cephalosporins, piperacillin-tazobactam, amikacin, gentamicin and tigecycline, except aztreonam. All isolates were positive for EDTA/SMA and NDM-1, and negative for APB and other carbapenemases. Two genetic groups, group 1 (n=9 isolates), group 2 (n=4 isolates) and an isolate defined as not genetically related.
CONCLUSION
This work describes the circulating of NDM-1-producing P. rettgeri in Colombia.
Topics: Adolescent; Adult; Aged; Aged, 80 and over; Anti-Bacterial Agents; Bacterial Proteins; Bacterial Typing Techniques; Colombia; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae Infections; Female; Genes, Bacterial; Genotype; Humans; Male; Middle Aged; Providencia; Young Adult; beta-Lactam Resistance; beta-Lactamases
PubMed: 26130312
DOI: 10.1016/j.eimc.2015.05.011 -
Infection and Immunity Dec 1992Providencia alcalifaciens is a member of the family Enterobacteriaceae. There are reports that P. alcalifaciens can cause diarrhea, but the mechanism(s) by which it...
Providencia alcalifaciens is a member of the family Enterobacteriaceae. There are reports that P. alcalifaciens can cause diarrhea, but the mechanism(s) by which it causes diarrhea is known. We studied P. alcalifaciens isolated from a child and two adults with diarrhea for enteropathogenicity. The three isolates did not exhibit any characteristic adherence to cultured HEp-2 cell monolayers, and they did not produce enterotoxins, cytotoxins, or keratoconjunctivitis in the Sereny test. Two isolates invaded cultured HEp-2 cell monolayers, producing localized bacterial clusters and actin condensation. The pattern of actin condensation was different from that produced by enteropathogenic Escherichia coli but similar to that produced by Shigella flexneri. Invasion and actin condensation were poor for the third isolate. Histology of adult rabbit small intestinal loops inoculated with all three isolates revealed bacterial attachment to, penetration of, and microulcer formation on the surface epithelium and hyperemia, edema, and polymorphonuclear cell infiltration of lamina propria. All the isolates produced diarrhea in rabbits with removable intestinal ties, and some of these rabbits developed hindlimb paralysis. Intestinal histology of the rabbits with removable intestinal ties which developed diarrhea showed changes similar to that in adult rabbits on which ileal loop assays had been performed. Transmission electron microscopy of intestinal tissues also confirmed tissue penetration by the isolates. Nerve tissue histology of two rabbits that developed hindlimb paralysis showed focal mononuclear cell infiltration around peripheral nerve sheaths. It is concluded that some strains of P. alcalifaciens are enteropathogenic and that they cause diarrhea by invading the intestinal mucosal epithelium. However, the relevance to human disease of the hindlimb paralysis observed in this animal model is not clear.
Topics: Animals; Diarrhea; Intestines; Paralysis; Providencia; Rabbits
PubMed: 1452332
DOI: 10.1128/iai.60.12.5017-5024.1992 -
Frontiers in Cellular and Infection... 2021is a nosocomial pathogen associated with urinary tract infections related to hospital-acquired Infections. In recent years, clinical strains producing New Delhi...
is a nosocomial pathogen associated with urinary tract infections related to hospital-acquired Infections. In recent years, clinical strains producing New Delhi Metallo-β-lactamase (NDM) and other β-lactamase which reduce the efficiency of antimicrobial therapy have been reported. However, there are few reports of co-producing two metallo-β-lactamases in one isolate. Here, we first reported a strain (P138) co-harboring , , and . The specie were identified using MALDI-TOF MS. The results of antimicrobial susceptibility testing by broth microdilution method indicated that P138 was resistant to meropenem (MIC = 64μg/ml), imipenem (MIC = 64μg/ml), and aztreonam (MIC = 32μg/ml). Conjugation experiments revealed that the -carrying plasmid was transferrable. The carbapenemase genes were detected using PCR and confirmed by PCR-based sequencing. The complete genomic sequence of the was identified using Illumina (Illumina, San Diego, CA, USA) short-read sequencing (150bp paired-end reads), and many common resistance genes had been identified, including , , , , , , and . The gene was characterized by the following structure: IS-TnpA-IntI1-aadB-IS-GroEL-GroES-DsbD-PAI-ble--IS-QnrS1-IS. Blast comparison revealed that the gene structure shared >99% similarity with plasmid p5_SCLZS62 (99% nucleotide identity and query coverage). In summary, we isolated a strain coproducing , , and blaOXA-10. To the best of our acknowledge, this was first reported in the world. The occurrence of the strain needs to be closely monitored.
Topics: Anti-Bacterial Agents; China; Enterobacteriaceae Infections; Humans; Microbial Sensitivity Tests; Providencia; beta-Lactamases
PubMed: 35047418
DOI: 10.3389/fcimb.2021.789646 -
PloS One 2017Biofilms are organized communities of bacterial cells that are responsible for the majority of human chronic bacterial infections. Providencia stuartii is a...
Biofilms are organized communities of bacterial cells that are responsible for the majority of human chronic bacterial infections. Providencia stuartii is a Gram-negative biofilm-forming bacterium involved in high incidence of urinary tract infections in catheterized patients. Yet, the structuration of these biofilms, and their resistance to environmental insults remain poorly understood. Here, we report on planktonic cell growth and biofilm formation by P. stuartii, in conditions that mimic its most common pathophysiological habitat in humans, i.e. the urinary tract. We observed that, in the planktonic state, P. stuartii forms floating communities of cells, prior to attachment to a surface and subsequent adoption of the biofilm phenotype. P. stuartii planktonic and biofilm cells are remarkably resistant to calcium, magnesium and to high concentrations of urea, and show the ability to grow over a wide range of pHs. Experiments conducted on a P. stuartii strain knocked-out for the Omp-Pst2 porin sheds light on the role it plays in the early stages of growth, as well as in the adaptation to high concentration of urea and to varying pH.
Topics: Biofilms; Calcium; Environment; Gene Knockdown Techniques; Hydrogen-Ion Concentration; Magnesium; Providencia; Urea
PubMed: 28334028
DOI: 10.1371/journal.pone.0174213 -
Lancet (London, England) Nov 1975
Topics: Aminoglycosides; Cross Infection; Drug Resistance, Microbial; Humans; Proteus; Proteus Infections; Providencia
PubMed: 53535
DOI: 10.1016/s0140-6736(75)90341-4 -
International Journal of Systematic and... Mar 2006In the course of isolating bacteria from infective juveniles of the entomopathogenic nematode Steinernema thermophilum Ganguly & Singh, 2000, three isolates were...
In the course of isolating bacteria from infective juveniles of the entomopathogenic nematode Steinernema thermophilum Ganguly & Singh, 2000, three isolates were obtained (OP1T, OP29 and VS3). On the basis of 16S rRNA gene sequence analysis and riboprint patterns, these three strains were identical to each other but distinct from the type strains of the five recognized species of the genus Providencia. Based on biochemical and genomic analysis and supported by the low (<35 %) DNA-DNA relatedness between strain OP1T and the type strain of its phylogenetically closest relative, Providencia rettgeri (99.5 % 16S rRNA gene sequence similarity), strain OP1T was considered to be sufficiently distinct from recognized Providencia species to warrant the description of a novel species. The name Providencia vermicola sp. nov. is proposed, with OP1T (= DSM 17385T = CIP 108829T) as the type strain.
Topics: Animals; DNA, Ribosomal; Nematoda; Phylogeny; Providencia; RNA, Ribosomal, 16S; Ribotyping; Sequence Analysis, DNA
PubMed: 16514040
DOI: 10.1099/ijs.0.63973-0