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Australian Veterinary Journal Nov 1996
Topics: Alligators and Crocodiles; Animal Diseases; Animals; Animals, Newborn; Brain; Enterobacteriaceae Infections; Kidney; Liver; Meningitis, Bacterial; Providencia; Queensland
PubMed: 8941426
DOI: 10.1111/j.1751-0813.1996.tb15456.x -
Journal of Global Antimicrobial... Sep 2022
Topics: beta-Lactamases; Colombia; Providencia
PubMed: 35569756
DOI: 10.1016/j.jgar.2022.05.005 -
Journal of Medical Microbiology Nov 2005In this study the importance of Providencia species as a cause of travellers' diarrhoea was examined using a selective medium developed by the authors. Providencia...
In this study the importance of Providencia species as a cause of travellers' diarrhoea was examined using a selective medium developed by the authors. Providencia species could easily be distinguished from other enteric pathogens by the colour of the colonies obtained. Nine strains of Providencia alcalifaciens, nine of Providencia rettgeri and five of Providencia stuartii were isolated from 130 specimens, representing a surprisingly high incidence of infection compared with other pathogens isolated on SS agar and TCBS agar. Patients infected with P. rettgeri complained of abdominal pain, as for other Providencia species, but also of vomiting, which is rather characteristic of P. rettgeri infection. To analyse the pathogenicity of these isolates, their invasiveness was examined using Caco-2 cells. Most of the P. rettgeri strains invaded Caco-2 cells. Random amplified polymorphic DNA (RAPD) fingerprinting showed the same profile for two P. rettgeri isolates from individuals travelling in the same tour group. The results show that Providencia species, especially P. rettgeri, might cause diarrhoea, and that these species are important pathogens.
Topics: Abdominal Pain; Adolescent; Adult; Aged; Caco-2 Cells; Culture Media; DNA Fingerprinting; DNA, Bacterial; Diarrhea; Enterobacteriaceae Infections; Feces; Humans; Middle Aged; Molecular Epidemiology; Providencia; Random Amplified Polymorphic DNA Technique; Travel; Vomiting
PubMed: 16192440
DOI: 10.1099/jmm.0.45846-0 -
The Journal of Infectious Diseases Mar 2020Imipenemase (IMP) is a metallo-β-lactamase that confers resistance to almost all β-lactams. Identification of IMP genes is essential for understanding and combatting...
A Pandrug-Resistant Providencia Carrying Two blaIMP Carbapenemase-Encoding Genes Including blaIMP-69, a New blaIMP Variant, on a Newly Identified Worldwide-Distributed IncC Plasmid.
Imipenemase (IMP) is a metallo-β-lactamase that confers resistance to almost all β-lactams. Identification of IMP genes is essential for understanding and combatting antibiotic resistance. In this study, we report a pandrug-resistant Providencia strain from a human rectal swab. This strain carried 2 blaIMP carbapenemase genes, blaIMP-69 and blaIMP-4. IMP-69 is a novel IMP variant with an amino acid substitution at A21T compared with IMP-8. blaIMP-69 was found in a blaIMP-69-aacA4 array of an integron on a 165-kilobase (kb) IncC self-transmissible plasmid, whereas blaIMP-4 was located in a blaIMP-4-qacG-aacA4-catB3 array of an integron on a 19-kb nonself-transmissible plasmid. Such coexistence has the potential to allow the generation of new, hybrid blaIMP variants by homologous recombination. The blaIMP-69-carrying IncC plasmid belonged to the core-genome plasmid multilocus sequence typing (cgPMLST) 3.5 type. We found that cgPMLST 3.5 IncC plasmids have been circulating worldwide for decades and may represent a common vehicle mediating the spread of antimicrobial resistance.
Topics: Bacterial Proteins; Carbapenems; Drug Resistance, Multiple, Bacterial; Humans; Integrons; Microbial Sensitivity Tests; Multilocus Sequence Typing; Plasmids; Providencia; beta-Lactamases
PubMed: 32176782
DOI: 10.1093/infdis/jiz476 -
Diagnostic Microbiology and Infectious... May 2024Providencia rettgeri, belonging to the genus Providencia, had gained significant interest due to its increasing prevalence as a common pathogen responsible for...
Providencia rettgeri, belonging to the genus Providencia, had gained significant interest due to its increasing prevalence as a common pathogen responsible for healthcare-associated infections in hospitals. P. rettgeri isolates producing carbapenemases have been reported to reduce the efficiency of carbapenems in clinical antimicrobial therapy. However, coexistence with other resistance determinants is rarely reported. The goal of this study was the molecular characterization of carbapenemase-producing Providencia spp. clinical isolates. Among 23 Providencia spp. resistant to imipenem, 21 were positive to bla; one positive to bla and bla; and one isolate co-producing bla, bla, and bla were identified. We observed a low clonal relationship, and the incompatibility groups Col3M and ColRNAI were identified in the plasmid harboring bla. We report for the first time a P. rettgeri strain co-producing bla, bla, and bla. The analysis of these resistance mechanisms in carbapenemase co-producing clinical isolates reflects the increased resistance.
Topics: Humans; Anti-Bacterial Agents; Providencia; Mexico; Microbial Sensitivity Tests; beta-Lactamases; Bacterial Proteins
PubMed: 38452556
DOI: 10.1016/j.diagmicrobio.2024.116246 -
Genome Biology and Evolution Sep 2023Strict blood-feeding animals are confronted with a strong B-vitamin deficiency. Blood-feeding leeches from the Glossiphoniidae family, similarly to hematophagous...
Strict blood-feeding animals are confronted with a strong B-vitamin deficiency. Blood-feeding leeches from the Glossiphoniidae family, similarly to hematophagous insects, have evolved specialized organs called bacteriomes to harbor symbiotic bacteria. Leeches of the Haementeria genus have two pairs of globular bacteriomes attached to the esophagus which house intracellular "Candidatus Providencia siddallii" bacteria. Previous work analyzing a draft genome of the Providencia symbiont of the Mexican leech Haementeria officinalis showed that, in this species, the bacteria hold a reduced genome capable of synthesizing B vitamins. In this work, we aimed to expand our knowledge on the diversity and evolution of Providencia symbionts of Haementeria. For this purpose, we sequenced the symbiont genomes of three selected leech species. We found that all genomes are highly syntenic and have kept a stable genetic repertoire, mirroring ancient insect endosymbionts. Additionally, we found B-vitamin pathways to be conserved among these symbionts, pointing to a conserved symbiotic role. Lastly and most notably, we found that the symbiont of H. acuecueyetzin has evolved an alternative genetic code, affecting a portion of its proteome and showing evidence of a lineage-specific and likely intermediate stage of genetic code reassignment.
Topics: Animals; Providencia; Phylogeny; Leeches; Bacteria; Insecta; Vitamins; Genetic Code; Symbiosis
PubMed: 37690114
DOI: 10.1093/gbe/evad164 -
Microbial Drug Resistance (Larchmont,... Jul 2016Providencia stuartii has emerged as an important nosocomial pathogen. We describe an outbreak due to a multidrug-resistant strain over a 4-month period in a critical...
Providencia stuartii Isolates from Greece: Co-Carriage of Cephalosporin (blaSHV-5, blaVEB-1), Carbapenem (blaVIM-1), and Aminoglycoside (rmtB) Resistance Determinants by a Multidrug-Resistant Outbreak Clone.
Providencia stuartii has emerged as an important nosocomial pathogen. We describe an outbreak due to a multidrug-resistant strain over a 4-month period in a critical care unit in Athens. Molecular typing revealed each of the isolates to be clonally related with coresistance to cephalosporins, carbapenems, aminoglycosides, and quinolones. Each isolate contained a 220-kb multi-replicon (IncA/C and IncR) conjugative plasmid encoding TEM-1, SHV-5, VEB-1, and VIM-1 β-lactamases and the 16S rDNA methylase RmtB. Antimicrobial therapy was unsuccessful in 3 of 6 cases, and resistance was readily transmissible to susceptible strains of Escherichia coli by transformation and conjugation. This highlights the clinical importance of P. stuartii and its ability to disseminate critical resistance determinants to other bacterial pathogens.
Topics: Adult; Aged; Aminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; Carbapenems; Cephalosporins; Clone Cells; Conjugation, Genetic; Cross Infection; Disease Outbreaks; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae Infections; Female; Gene Expression; Gene Transfer, Horizontal; Greece; Humans; Male; Methyltransferases; Microbial Sensitivity Tests; Middle Aged; Plasmids; Providencia; Quinolones; Replicon; Survival Analysis; beta-Lactamases
PubMed: 27380549
DOI: 10.1089/mdr.2015.0215 -
Journal of Microbiological Methods Feb 2021Cytolethal distending toxins (CDTs) produced by P. alcalifaciens are considered as potential virulence factors. A loop-mediated isothermal amplification (LAMP) method...
Cytolethal distending toxins (CDTs) produced by P. alcalifaciens are considered as potential virulence factors. A loop-mediated isothermal amplification (LAMP) method for the detection of cdtA, cdtB, and cdtC genes was established which showed high specificity and strong sensitivity. The LAMP assay showed a detection threshold was 3.13 pg/μl within 40 min.
Topics: Bacterial Toxins; Enterobacteriaceae Infections; Humans; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques; Providencia; Virulence Factors
PubMed: 33428969
DOI: 10.1016/j.mimet.2021.106143 -
The Journal of International Medical... Oct 2020To investigate the clinical and drug resistance characteristics of infections in the Huainan region of Anhui and provide a reference for the clinical selection of...
OBJECTIVES
To investigate the clinical and drug resistance characteristics of infections in the Huainan region of Anhui and provide a reference for the clinical selection of antimicrobial agents.
METHODS
This single-center retrospective analysis included 76 patients with infection in Huainan during the period from October 2018 to March 2020. The hospital department in which the patients were treated and the drug susceptibility characteristics of the isolates were recorded.
RESULTS
Among the 76 patients, the lung was the most common site of infection, and intensive care unit was the main hospital department. Extended spectrum beta-lactamase screening revealed expression by all 76 isolates of . Of the 76 isolates, 92.1% exhibited multiple drug resistance or extensive drug resistance. isolates were sensitive to cefepime and imipenem, but not to other beta-lactam antibiotics. Twenty isolates were resistant to all 21 types of antibiotics. Of the 20 patients infected with extensively drug-resistant isolates, nine (45%) died.
CONCLUSIONS
Drug resistance is increasing in . The antimicrobial agent imipenem may be effective for treatment of infections. Fluoroquinolones, aminoglycosides, and fourth-generation cephalosporins are suitable options for antibiotic therapy.
Topics: Anti-Bacterial Agents; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae Infections; Humans; Microbial Sensitivity Tests; Providencia; Retrospective Studies
PubMed: 33081537
DOI: 10.1177/0300060520962296 -
Shokuhin Eiseigaku Zasshi. Journal of... Aug 2005
Review
Topics: Antibodies, Bacterial; Biomarkers; DNA, Bacterial; Disease Outbreaks; Food Microbiology; Foodborne Diseases; Humans; Japan; Nucleic Acid Amplification Techniques; Providencia
PubMed: 16180691
DOI: No ID Found