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The Journal of General and Applied... 2013The bacterial strain C1112(T) was isolated from seafood processing wastewater collected from a treatment pond of the seafood factory in Songkhla Province, Thailand....
The bacterial strain C1112(T) was isolated from seafood processing wastewater collected from a treatment pond of the seafood factory in Songkhla Province, Thailand. Phylogenetic analysis based on concatenated sequences from the 16S rRNA gene and five housekeeping genes, fusA, lepA, leuS, gyrB and ileS respectively showed that the strain C1112(T) belonged to the genus Providencia, and share 91.75% similarity with P. stuartii DSM 4539(T). DNA-DNA hybridization between the strain C1112(T) and P. stuartii KCTC 2568(T) was 48.1% relatedness. Moreover, some results from biochemical properties indicated that the strain C1112(T) was distinguished from the phylogenetically closest relatives. The major fatty acids of the strain C1112(T) were C16:0, iso-C15:0, C14:0 and C17:0 cyclo and the DNA G+C content was 41 mol%. Based on the genotypic and phenotypic considerations, it should be classified as a novel species of the genus Providencia for which the name Providencia thailandensis sp. nov. is proposed. The type strain is C1112(T) (= KCTC 23281(T) =NBRC 106720(T)).
Topics: Bacterial Proteins; Bacterial Typing Techniques; Base Composition; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Industrial Waste; Microscopy, Phase-Contrast; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; Providencia; RNA, Ribosomal, 16S; Seafood; Sequence Analysis, DNA; Thailand; Wastewater
PubMed: 23863288
DOI: 10.2323/jgam.59.185 -
The Journal of Hospital Infection Jun 1981
Topics: Adolescent; Adult; Aged; Anti-Bacterial Agents; Child; Child, Preschool; Drug Resistance, Microbial; Female; Gentamicins; Humans; Infant; Male; Middle Aged; Proteus; Proteus Infections; Providencia; Pseudomonas aeruginosa; Sepsis; Skin Diseases, Infectious; Sputum; Urinary Tract Infections
PubMed: 6174578
DOI: 10.1016/0195-6701(81)90024-4 -
The Journal of Hospital Infection Oct 2019Several Romanian hospitals have noted increasing isolation of Providencia stuartii strains in recent years, with an alarming rate of carbapenem resistance. In order to...
Several Romanian hospitals have noted increasing isolation of Providencia stuartii strains in recent years, with an alarming rate of carbapenem resistance. In order to provide molecular epidemiological data regarding their dissemination, 77 P. stuartii strains collected from five hospitals located in different regions of Romania were analysed. All strains harboured IncA/C plasmid, and 67 carried the blaNDM-1 gene. Six clonal clusters were differentiated by pulsed-field gel electrophoresis. The predominant subtype was found in all five hospitals. Our study highlights the need for efficient infection-control measures, the optimization of antibiotic use and the targeted surveillance for carbapenemase-producing P. stuartii.
Topics: Cross Infection; Enterobacteriaceae Infections; Genotype; Hospitals; Humans; Molecular Epidemiology; Molecular Typing; Plasmids; Providencia; Romania; beta-Lactamases
PubMed: 31039380
DOI: 10.1016/j.jhin.2019.04.015 -
Antimicrobial Agents and Chemotherapy Apr 2001The sequences of the bla(TEM) genes encoding TEM-92 in Proteus mirabilis and Providencia stuartii isolates were determined and were found to be identical. Except for...
The sequences of the bla(TEM) genes encoding TEM-92 in Proteus mirabilis and Providencia stuartii isolates were determined and were found to be identical. Except for positions 218 (Lys-6) and 512 (Lys-104), the nucleotide sequence of bla(TEM-92) was identical to that of bla(TEM-20), including the sequence of the promoter region harboring a 135-bp deletion combined with a G-162-->T substitution. The deduced amino acid sequence of TEM-92 differed from that of TEM-52 by the presence of a substitution (Gln-6-->Lys) in the peptide signal.
Topics: Amino Acid Substitution; Enterobacteriaceae Infections; Genes, Bacterial; Humans; Microbial Sensitivity Tests; Promoter Regions, Genetic; Proteus Infections; Proteus mirabilis; Providencia; Sequence Analysis, DNA; beta-Lactam Resistance; beta-Lactamases
PubMed: 11257046
DOI: 10.1128/AAC.45.4.1278-1280.2001 -
Journal of Medical Microbiology Aug 2003The ability of Providencia alcalifaciens strains, isolated from patients with diarrhoeal disease, to translocate from the gastrointestinal tract and their resistance to...
The ability of Providencia alcalifaciens strains, isolated from patients with diarrhoeal disease, to translocate from the gastrointestinal tract and their resistance to serum complement lytic activity were investigated and compared with previously characterized differential invasive capabilities in HeLa cells. Translocation ability to several extraintestinal sites and resistance to lysis by human serum complement were observed in both highly invasive and non-invasive strains. These characteristics have not been previously described in P. alcalifaciens and their potential role in causing disseminated infections should therefore be considered.
Topics: Complement System Proteins; Diarrhea; Digestive System; Enterobacteriaceae Infections; Feces; HeLa Cells; Humans; Providencia
PubMed: 12867555
DOI: 10.1099/jmm.0.04993-0 -
Proceedings of the National Academy of... Mar 2018The gram-negative pathogen forms floating communities within which adjacent cells are in apparent contact, before depositing as canonical surface-attached biofilms....
The gram-negative pathogen forms floating communities within which adjacent cells are in apparent contact, before depositing as canonical surface-attached biofilms. Because porins are the most abundant proteins in the outer membrane of gram-negative bacteria, we hypothesized that they could be involved in cell-to-cell contact and undertook a structure-function relationship study on the two porins of , Omp-Pst1 and Omp-Pst2. Our crystal structures reveal that these porins can self-associate through their extracellular loops, forming dimers of trimers (DOTs) that could enable cell-to-cell contact within floating communities. Support for this hypothesis was obtained by studying the porin-dependent aggregation of liposomes and model cells. The observation that facing channels are open in the two porin structures suggests that DOTs could not only promote cell-to-cell contact but also contribute to intercellular communication.
Topics: Bacterial Outer Membrane Proteins; Biofilms; Crystallography, X-Ray; Dimerization; Porins; Providencia
PubMed: 29476011
DOI: 10.1073/pnas.1714582115 -
Food Additives & Contaminants. Part A,... 2013Providencia alcalifaciens is a member of the Enterobacteriaceae family that occasionally causes diarrheagenic illness in humans via the intake of contaminated foods....
Providencia alcalifaciens is a member of the Enterobacteriaceae family that occasionally causes diarrheagenic illness in humans via the intake of contaminated foods. Despite the epidemiological importance of P. alcalifaciens, little is known about its pathobiology. Here we report that P. alcalifaciens causes barrier dysfunction in Caco-2 cell monolayers and induces apoptosis in calf pulmonary artery endothelial cells. P. alcalifaciens infection caused a 30% reduction in transepithelial resistance in Caco-2 cell monolayers, which was greater than that for cells infected with Shigella flexneri or non-pathogenic Escherichia coli. As with viable bacteria, bacterial lysates treated with heat, benzonase or proteinase, but not with polymixin B, were also involved in the cellular response. TLR4 antibody neutralisation significantly restored the P. alcalifaciens-induced transepithelial resistance reduction in Caco-2 cells, suggesting that lipopolysaccharides (LPSs) might play a central role in this cellular response. Western blotting further indicated that P. alcalifaciens LPSs reduced occludin levels, whereas LPSs from Shigella or E. coli did not. Although the viability of Caco-2 cells was not altered significantly, the calf pulmonary artery endothelial cell line was highly sensitive to P. alcalifaciens infection. This sensitivity was indeed dependent on LPS, which induced rapid apoptosis. Together, these data show that P. alcalifaciens LPSs participate in epithelial barrier dysfunction and endothelial apoptosis. The findings give insight into the LPS-dependent cell signal events affecting diarrheagenicity during infection with P. alcalifaciens.
Topics: Apoptosis; Caco-2 Cells; Cell Culture Techniques; Cell Survival; Diarrhea; Humans; Lipopolysaccharides; Providencia
PubMed: 23710559
DOI: 10.1080/19440049.2013.790086 -
Journal of Microbiology (Seoul, Korea) Jun 2007During May to July 2004, three strains of Providencia spp. with multidrug-resistance (MDR) were isolated from urinary specimen of three patients hospitalized with a same...
During May to July 2004, three strains of Providencia spp. with multidrug-resistance (MDR) were isolated from urinary specimen of three patients hospitalized with a same hospital room. By PCR analysis, all three strains have been found to carry both VIM-2 type metallo-beta-lactamase gene and PER-1 type extendedspectrum beta-lactamase gene. One out of three strains carried additional resistance gene, armA, 16S rRNA methylase gene responsible for high level resistance to aminoglycosides. To our knowledge, this is the first report on the identification of Providencia spp. simultaneously carrying blaVIM-2, blaPER-1, and armA genes.
Topics: Anti-Bacterial Agents; Bacterial Proteins; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae Infections; Humans; Methyltransferases; Microbial Sensitivity Tests; Polymerase Chain Reaction; Providencia; Urinary Tract Infections; beta-Lactamases
PubMed: 17618235
DOI: No ID Found -
Journal of Clinical Microbiology May 1998It has been demonstrated in previous studies that Providencia alcalifaciens can produce diarrhea by an invasive mechanism. In the present study, P. alcalifaciens was...
It has been demonstrated in previous studies that Providencia alcalifaciens can produce diarrhea by an invasive mechanism. In the present study, P. alcalifaciens was isolated from the stool specimens of 17 of 814 diarrheal children younger than 5 years of age (2.1%) and from those of 4 of 814 matched controls (0.49%) (P = 0.004), indicating that the organism is significantly associated with diarrhea. However, 71% of P. alcalifaciens-positive diarrheal children had simultaneous infections with other recognized enteric pathogens.
Topics: Child, Preschool; Diarrhea; Enterobacteriaceae Infections; Feces; Female; Humans; Infant; Male; Providencia
PubMed: 9574723
DOI: 10.1128/JCM.36.5.1433-1435.1998 -
Journal of Environmental Sciences... Oct 2024Tri (2-chloropropyl) phosphate (TCPP) was an emerging contaminant of global concern because of its frequent occurrence, potential toxic effects, and persistence in the...
Tri (2-chloropropyl) phosphate (TCPP) was an emerging contaminant of global concern because of its frequent occurrence, potential toxic effects, and persistence in the environment. Microbial degradation might be an efficient and safe removal method, but limited information was available. In this study, Providencia rettgeri was isolated from contaminated sediment and showed it could use TCPP as unique phosphorus source to promote growth, and decompose 34.7% of TCPP (1 mg/L) within 5 days. The microbial inoculation and the initial concentration of TCPP could affect the biodegradation efficient. Further study results indicated that TCPP decomposition by Providencia rettgeri was mainly via phosphoester bond hydrolysis, evidenced by the production of bis (2-chloropropyl) phosphate (CHClPO) and mono-chloropropyl phosphate (CHClPO). Both intracellular and extracellular enzymes could degrade TCPP, but intracellular degradation was dominant in the later reaction stage, and the presence of Cu ions had a promoting effect. These findings developed novel insights into the potential mechanism of TCPP microbial degradation.
Topics: Providencia; Biodegradation, Environmental; Phosphates; Water Pollutants, Chemical
PubMed: 38802235
DOI: 10.1016/j.jes.2023.07.023