-
BMC Microbiology Nov 2020Providencia rettgeri is a nosocomial pathogen associated with urinary tract infections and related to Healthcare-Associated Infection (HAI). In recent years isolates...
BACKGROUND
Providencia rettgeri is a nosocomial pathogen associated with urinary tract infections and related to Healthcare-Associated Infection (HAI). In recent years isolates producing New Delhi Metallo-β-lactamase (NDM) and other β-lactamases have been reported that reduce the efficiency of clinical antimicrobial treatments. In this study, we analyzed antibiotic resistance, the presence of resistance genes and the clonal relationship of two P. rettgeri isolates obtained from male patients admitted to the same hospital in Bogotá - Colombia, 2015.
RESULTS
Antibiotic susceptibility profile evaluated by the Kirby-Bauer method revealed that both isolates were resistant to third-generation carbapenems and cephalosporins. Whole-genome sequencing (Illumina HiSeq) followed by SPAdes assembling, Prokka annotation in combination with an in-house Python program and resistance gene detection by ResFinder identified the same six β-lactamase genes in both isolates: bla, bla, bla, bla, bla and bla. Additionally, various resistance genes associated with antibiotic target alteration (arnA, PmrE, PmrF, LpxA, LpxC, gyrB, folP, murA, rpoB, rpsL, tet34) were found and four efflux pumps (RosAB, EmrD, mdtH and cmlA). The additional resistance to gentamicin in one of the two isolates could be explained by a detected SNP in CpxA (Cys191Arg) which is involved in the stress response of the bacterial envelope. Genome BLAST comparison using CGView, the ANI value (99.99%) and the pangenome (using Roary) phylogenetic tree (same clade, small distance) showed high similarity between the isolates. The rMLST analysis indicated that both isolates were typed as rST-61,696, same as the RB151 isolate previously isolated in Bucaramanga, Colombia, 2013, and the FDAARGOS_330 isolate isolated in the USA, 2015.
CONCLUSIONS
We report the coexistence of the carbapenemase genes bla, and bla, together with the β-lactamase genes bla, bla, bla and bla, in P. rettgeri isolates from two patients in Colombia. Whole-genome sequence analysis indicated a circulation of P. rettgeri rST-61,696 strains in America that needs to be investigated further.
Topics: Anti-Bacterial Agents; Bacterial Proteins; Colombia; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae Infections; Genome, Bacterial; Humans; Male; Microbial Sensitivity Tests; Providencia; beta-Lactam Resistance; beta-Lactamases
PubMed: 33183231
DOI: 10.1186/s12866-020-02030-z -
Journal of Bacteriology Jul 2012Here we present the complete genome sequence of Providencia stuartii MRSN 2154, isolated from an Afghan national. P. stuartii is a Gram-negative bacillus capable of...
Here we present the complete genome sequence of Providencia stuartii MRSN 2154, isolated from an Afghan national. P. stuartii is a Gram-negative bacillus capable of causing infections in a wide variety of human tissues. Because Providencia readily acquires plasmids bearing drug resistance loci, it is of growing clinical significance.
Topics: Enterobacteriaceae Infections; Gene Expression Regulation, Bacterial; Genome, Bacterial; Humans; Molecular Sequence Data; Providencia
PubMed: 22740665
DOI: 10.1128/JB.00615-12 -
The Journal of Antimicrobial... Aug 2013
Topics: Adolescent; Anti-Bacterial Agents; Carbapenems; Enterobacteriaceae Infections; Female; Humans; Male; Mexico; Microbial Sensitivity Tests; Middle Aged; Plasmids; Providencia; Young Adult; beta-Lactam Resistance; beta-Lactamases
PubMed: 23620464
DOI: 10.1093/jac/dkt124 -
The Journal of Antimicrobial... Dec 2013
Topics: Anti-Bacterial Agents; Brazil; DNA Transposable Elements; DNA, Bacterial; Enterobacteriaceae Infections; Humans; Male; Microbial Sensitivity Tests; Providencia; Sequence Analysis, DNA; beta-Lactamases
PubMed: 23869051
DOI: 10.1093/jac/dkt298 -
Environmental Technology Sep 2016Providencia rettgeri strain YL shows the capability of nitrogen removal under sole aerobic conditions. By using isotope ratio mass spectrometry, (15)N-labelled N2O and...
Providencia rettgeri strain YL shows the capability of nitrogen removal under sole aerobic conditions. By using isotope ratio mass spectrometry, (15)N-labelled N2O and N2 were detected in aerobic batch cultures containing [Formula: see text], [Formula: see text] or [Formula: see text]. Strain YL converted [Formula: see text], [Formula: see text] and [Formula: see text] to produce more N2O than N2 in the presence of [Formula: see text]. An (15)N isotope tracing experiment confirmed that the nitrogen removal pathway of strain YL was heterotrophic nitrification-aerobic denitrification. The optimal treatment conditions for nitrogen removal were pH of 8, C/N ratio of 12, temperature of 25°C and shaking speed of 105 rpm. A continuous aerobic bioreactor inoculated with strain YL was developed. With an influent [Formula: see text] concentration of 90-200 mg/L, the [Formula: see text] removal efficiency ranged from 80% to 97% and the total nitrogen removal efficiency ranged from 72% to 95%. The nitrogen balance in the continuous bioreactor revealed that approximately 35-52% of influent [Formula: see text] was denitrified aerobically to form gaseous nitrogen. These findings show that the P. rettgeri strain YL has potential application in wastewater treatment for nitrogen removal under sole aerobic conditions.
Topics: Aerobiosis; Ammonium Compounds; Bioreactors; Denitrification; Heterotrophic Processes; Nitrogen; Nitrogen Isotopes; Providencia; Wastewater; Water Purification
PubMed: 26824874
DOI: 10.1080/09593330.2016.1146338 -
Infection Control and Hospital... Jun 2012Outbreaks by carbapenem-resistant Providencia stuartii (CRPS) are rarely described. Clinical characteristics of patients with CRPS in an intensive care unit and...
Outbreaks by carbapenem-resistant Providencia stuartii (CRPS) are rarely described. Clinical characteristics of patients with CRPS in an intensive care unit and resistance mechanisms were investigated. Carbapenemase production and/or outer membrane alterations were not detected; only CTX-M-2 and AmpC hyperproduction were noted. The outbreak was ultimately controlled in a 3-month period.
Topics: Adolescent; Adult; Anti-Bacterial Agents; Bacterial Proteins; Biomarkers; Brazil; Carbapenems; Cross Infection; Disease Outbreaks; Enterobacteriaceae Infections; Female; Humans; Infection Control; Intensive Care Units; Male; Microbial Sensitivity Tests; Middle Aged; Providencia; beta-Lactam Resistance; beta-Lactamases
PubMed: 22561721
DOI: 10.1086/665730 -
Journal of Industrial Microbiology &... Jun 2010Providencia rettgeri strain YL was found to be efficient in heterotrophic nitrogen removal under aerobic conditions. Maximum removal of NH(4) (+)-N occurred under the...
Providencia rettgeri strain YL was found to be efficient in heterotrophic nitrogen removal under aerobic conditions. Maximum removal of NH(4) (+)-N occurred under the conditions of pH 7 and supplemented with glucose as the carbon source. Inorganic ions such as Mg(2+), Mn(2+), and Zn(2+) largely influenced the growth and nitrogen removal efficiency. A quantitative detection of nitrogen gas by gas chromatography was conducted to evaluate the nitrogen removal by strain YL. From the nitrogen balance during heterotrophic growth with 180 mg/l of NH(4) (+)-N, 44.5% of NH(4) (+)-N was in the form of N(2) and 49.7% was found in biomass, with only a trace amount of either nitrite or nitrate. The utilization of nitrite and nitrate during the ammonium removal process demonstrated that the nitrogen removal pathway by strain YL was heterotrophic nitrification-aerobic denitrification. A further enzyme assay of nitrate reductase and nitrite reductase activity under the aerobic condition confirmed this nitrogen removal pathway.
Topics: Aerobiosis; Biomass; Carbon; Hydrogen-Ion Concentration; Ions; Molecular Sequence Data; Nitrate Reductase; Nitrates; Nitrite Reductases; Nitrites; Nitrogen; Phylogeny; Providencia; Quaternary Ammonium Compounds
PubMed: 20333440
DOI: 10.1007/s10295-010-0708-7 -
Bioresource Technology Jan 2021Azo dyes pose hazards to ecosystems and human health and the cosubstrate strategy has become the focus for the bioremediation of azo dyes. Herein, Brilliant Crocein...
Azo dyes pose hazards to ecosystems and human health and the cosubstrate strategy has become the focus for the bioremediation of azo dyes. Herein, Brilliant Crocein (BC), a model pollutant, was biodegraded by Providencia rettgeri domesticated from activated sludge. Additional ethanol, as a cosubstrate, could accelerate P. rettgeri growth and BC biodegradation, as reflected by the Gompertz models. This phenomenon was attributed to the smaller metabolites and greater number of potential pathways observed under the synergistic effect of ethanol. Genomic analysis of P. rettgeri showed that functional genes related to azo bond cleavage, redox reactions, ring opening and hydrolysis played crucial roles in azo dye biodegradation. Furthermore, the mechanism proposed was that ethanol might stimulate the production of additional reducing power via the expression of related genes, leading to the cleavage of azo bonds and aromatic rings. However, biodegradation without ethanol could only partly cleave the azo bonds.
Topics: Azo Compounds; Biodegradation, Environmental; Coloring Agents; Ecosystem; Ethanol; Genomics; Humans; Kinetics; Providencia
PubMed: 32979594
DOI: 10.1016/j.biortech.2020.124117 -
Protein Science : a Publication of the... Jun 2015The existence of N-formylated sugars in the O-antigens of Gram-negative bacteria has been known since the middle 1980s, but only recently have the biosynthetic pathways...
The existence of N-formylated sugars in the O-antigens of Gram-negative bacteria has been known since the middle 1980s, but only recently have the biosynthetic pathways for their production been reported. In these pathways, glucose-1-phosphate is first activated by attachment to a dTMP moiety. This step is followed by a dehydration reaction and an amination. The last step in these pathways is catalyzed by N-formyltransferases that utilize N(10) -formyltetrahydrofolate as the carbon source. Here we describe the three-dimensional structure of one of these N-formyltransferases, namely VioF from Providencia alcalifaciens O30. Specifically, this enzyme catalyzes the conversion of dTDP-4-amino-4,6-dideoxyglucose (dTDP-Qui4N) to dTDP-4,6-dideoxy-4-formamido-d-glucose (dTDP-Qui4NFo). For this analysis, the structure of VioF was solved to 1.9 Å resolution in both its apoform and in complex with tetrahydrofolate and dTDP-Qui4N. The crystals used in the investigation belonged to the space group R32 and demonstrated reticular merohedral twinning. The overall catalytic core of the VioF subunit is characterized by a six stranded mixed β-sheet flanked on one side by three α-helices and on the other side by mostly random coil. This N-terminal domain is followed by an α-helix and a β-hairpin that form the subunit:subunit interface. The active site of the enzyme is shallow and solvent-exposed. Notably, the pyranosyl moiety of dTDP-Qui4N is positioned into the active site by only one hydrogen bond provided by Lys 77. Comparison of the VioF model to that of a previously determined N-formyltransferase suggests that substrate specificity is determined by interactions between the protein and the pyrophosphoryl group of the dTDP-sugar substrate.
Topics: Bacterial Proteins; Catalytic Domain; Crystallography, X-Ray; Formyltetrahydrofolates; Hydroxymethyl and Formyl Transferases; Models, Molecular; Protein Conformation; Providencia
PubMed: 25752909
DOI: 10.1002/pro.2675 -
Journal of Clinical Microbiology Sep 1999Providencia heimbachae was first described in 1986. It has been isolated from penguin feces and an aborted bovine fetus. To date, there has been no reported isolation of...
Providencia heimbachae was first described in 1986. It has been isolated from penguin feces and an aborted bovine fetus. To date, there has been no reported isolation of this organism from human specimens. We now report the isolation of P. heimbachae from the stool of a 23-year-old woman with idiopathic diarrhea. The identity of the human strain was determined biochemically and by DNA relatedness to the type strain of P. heimbachae.
Topics: Adult; DNA, Bacterial; Feces; Female; Humans; Microbial Sensitivity Tests; Providencia
PubMed: 10449504
DOI: 10.1128/JCM.37.9.3048-3050.1999