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Journal of Chemotherapy (Florence,... Sep 2020species have been recognized to cause infections in immunocompromised individuals. The purpose of this study was to systemically review all published cases of... (Review)
Review
species have been recognized to cause infections in immunocompromised individuals. The purpose of this study was to systemically review all published cases of infections in humans and describe the epidemiology, microbiology, antimicrobial susceptibility, treatment and outcomes of these infections in humans. We performed a systematic review of PubMed (through 20 Octrober 2019) for studies providing epidemiological, clinical, microbiological as well as treatment data and outcomes of species infections. A total of 37 studies, containing data of 99 patients, were included in the analysis. The most common infections were those of the bloodstream in 74.7% (74 patients), musculoskeletal infections in 8.1% (8 patients), skin and soft tissue infections (SSTIs) and peritoneal dialysis-associated peritonitis in 6.1% (6 patients) each. Epidemiology of these infections differed, with bacteremias being more prevalent in patients with malignancy and central venous lines, musculoskeletal infections being more prevalent after orthopedic surgery, and SSTIs occurring without any reported underlying cause. Resistance to beta-lactams was very high with penicillin, piperacillin/tazobactam resistance and cephalosporin resistance at 96.6%, 90.7% and 77.8% respectively, while quinolone resistance was 9.1%. Quinolones, carbapenems and cephalosporins are the most common agents used for treatment, irrespectively of the infection site. Overall mortality was 3% (3 patients), with the mortality attributed to being at 1% (1 patient).
Topics: Antifungal Agents; Gram-Negative Bacterial Infections; Humans; Methylobacteriaceae
PubMed: 32619387
DOI: 10.1080/1120009X.2020.1785742 -
Journal of Pediatric Hematology/oncology Jul 2020Roseomonas gilardii is a Gram-negative coccobacillus identified in immunocompromised pediatric patients. A 5-year-old male with a history of HbSβ thalassemia status... (Review)
Review
Roseomonas gilardii is a Gram-negative coccobacillus identified in immunocompromised pediatric patients. A 5-year-old male with a history of HbSβ thalassemia status postsurgical splenectomy presented to the emergency department with fever. Blood cultures grew R. gilardii at 63 hours, but the patient had been discharged home at 48 hours. The patient was readmitted for repeat cultures and initiated on meropenem for 10 days as Roseomonas spp. are often resistant to third generation cephalosporins. R. gilardii is a rare cause of bacteremia in immunocompromised patients. Clinicians should consider Roseomonas in slow growing Gram-negative rod bacteremias, and consider meropenem as empiric coverage.
Topics: Bacteremia; Child, Preschool; Gram-Negative Bacterial Infections; Humans; Male; Methylobacteriaceae; Prognosis; Thalassemia
PubMed: 30951022
DOI: 10.1097/MPH.0000000000001476 -
BMC Infectious Diseases Feb 2019Roseomonas mucosa, as a Gram-negative coccobacilli, is an opportunistic pathogen that has rarely been reported in human infections. Here we describe a case of bacteremia... (Review)
Review
BACKGROUND
Roseomonas mucosa, as a Gram-negative coccobacilli, is an opportunistic pathogen that has rarely been reported in human infections. Here we describe a case of bacteremia in an infective endocarditis patient with systemic lupus erythematosus (SLE).
CASE PRESENTATIONS
A 44-year-old female patient with SLE suffered bacteremia caused by Roseomonas mucosa complicated with infective endocarditis (IE). The patient started on treatment with piperacillin-tazobactam and levofloxacin against Roseomonas mucosa, which was switched after 4 days to meropenem and amikacin for an additional 2 weeks. She had a favorable outcome with a 6-week course of intravenous antibiotic therapy.
DISCUSSION AND CONCLUSIONS
Roseomonas mucosa is rarely reported in IE patients; therefore, we report the case in order to improve our ability to identify this pathogen and expand the range of known bacterial causes of infective endocarditis.
Topics: Adult; Amikacin; Anti-Bacterial Agents; Bacteremia; Endocarditis; Endocarditis, Bacterial; Female; Gram-Negative Bacterial Infections; Humans; Levofloxacin; Lupus Erythematosus, Systemic; Methylobacteriaceae; Piperacillin, Tazobactam Drug Combination
PubMed: 30755159
DOI: 10.1186/s12879-019-3774-0 -
International Journal of Systematic and... Feb 2022Three pale-red-pigmented, Gram-stain-negative, coccobacilli-shaped, motile and strictly aerobic bacteria, strains MO17, MO41 and NPKOSM1, were isolated from rice paddy...
Three pale-red-pigmented, Gram-stain-negative, coccobacilli-shaped, motile and strictly aerobic bacteria, strains MO17, MO41 and NPKOSM1, were isolated from rice paddy soil. Colonies were circular with entire edges, convex and pale red. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains MO17, MO41 and NPKOSM1 belonged to the genus and were closely related to FW-3 (98.2 %), YC6724 (98.0 %), 5N26 (98.0 %), BUT-5 (97.8 %), YIM 78007 (97.7 %), TH-G33 (97.6 %) and DS-48 (96.8 %). The DNA-DNA hybridization values between strains MO17, MO41 and NPKOSM1 were 84-92 %, and the values between the three strains and their close phylogenetic relatives were also below 70 %. The major cellular fatty acids were C 7, C and summed feature 3 (C 7c and/or iso-C 2OH). The predominant respiratory quinone was identified as Q-10. The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified aminophospholipid and two unknown phospholipids. Based on their distinctive phenotypic, phylogenetic and chemotaxonomic characteristics, the three strains are considered to represent novel species of the genus for which the name sp. nov. is proposed. The type strain is MO17 (=KACC 19933=NBRC 114495).
Topics: Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Methylobacteriaceae; Oryza; Phospholipids; Phylogeny; Pigmentation; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Ubiquinone
PubMed: 35171088
DOI: 10.1099/ijsem.0.005251 -
The Pan African Medical Journal 2020Appendicitis is one of the most common abdominal conditions requiring emergency surgery. However, acute appendicitis in patients with leukemia is a rare condition. We... (Review)
Review
Appendicitis is one of the most common abdominal conditions requiring emergency surgery. However, acute appendicitis in patients with leukemia is a rare condition. We report herein the case of an 18-year-old female with acute lymphoblastic leukemia (ALL), who was hospitalized in hematology department because of abdominal pain and fever. Ultrasound (US) of the abdomen revealed appendicitis and the patients underwent open appendectomy. The patient recovered without complications and was discharged in a good condition. The day of the operation blood and peritoneal fluid cultures were taken and Roseomonas gilardii was detected and healed empirically. The correct diagnosis of appendicitis in patients with leukemia and their management is challenging for physicians. Very rare microorganisms can be detected in these patients.
Topics: Acute Disease; Adolescent; Appendectomy; Appendicitis; Diagnosis, Differential; Female; Gram-Negative Bacterial Infections; Humans; Leukemia; Methylobacteriaceae
PubMed: 33117477
DOI: 10.11604/pamj.2020.36.283.24834 -
Archives of Microbiology Jul 2022Three bacterial isolates, Gram-stain-negative, non-motile, coccobacilli-shaped bacteria, strains OP-27, OP-5 and OP-30, were isolated from rice paddy soil. Phylogenetic...
Three bacterial isolates, Gram-stain-negative, non-motile, coccobacilli-shaped bacteria, strains OP-27, OP-5 and OP-30, were isolated from rice paddy soil. Phylogenetic analyses based on 16S rRNA gene sequences revealed that three isolates belonged to the genus Roseomonas, showing the highest sequence similarities to Roseomonas sediminicola FW-3 (98.1%) and Roseomonas lacus TH-G33 (98.0%). The genome size of strain OP-27 was 5.2 Mb in a single contig with DNA G+C content of 71.2%. The genome included 5164 predicted protein-coding genes, as well as 48 tRNA, 4 rRNA and 4 mRNA genes. The average nucleotide identity value between strain OP-27 and type strains of related species of the genus Roseomonas were 81.1-83.1%, and the digital DNA-DNA hybridization values of strain OP-27 and the related strains were 24.6-26.8%, respectively. The DNA-DNA hybridization values between strains OP-27, OP-5 and OP-30 were 84-100% and its closest relative, Roseomonas sediminicola KACC 16616 was 21.1%. The major fatty acids were C ω7c, C 2-OH and C and predominant quinone was Q-10. Based on its distinctive phenotypic, phylogenetic, and chemotaxonomic characteristics, the three strains are considered to represent novel species of the genus Roseomonas, for which the name Roseomonas rosulenta sp. nov. is proposed. The type strain is OP-27 (=KACC 21501= NBRC 114497).
Topics: Bacterial Typing Techniques; DNA, Bacterial; Methylobacteriaceae; Oryza; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil
PubMed: 35778570
DOI: 10.1007/s00203-022-03014-5 -
International Journal of Systematic and... Feb 2023A non-motile, rod-shaped, pink-pigmented bacterium NAR14 was isolated from coral from Daya Bay, Shenzhen, PR China. Cells were Gram-stain-negative, aerobic,...
A non-motile, rod-shaped, pink-pigmented bacterium NAR14 was isolated from coral from Daya Bay, Shenzhen, PR China. Cells were Gram-stain-negative, aerobic, catalase-positive and oxidase-negative. NAR14 grew with 0-6 % (w/v) NaCl (optimum, 2-4 %), at 10-41 °C (optimum, 28 °C) and at pH 4.0-9.5 (optimum, 5.0). The major respiratory quinone was Q-10. The predominant fatty acids (more than 10%) were summed feature 8 (65.6 %) and C (17.6%). The DNA G+C content of NAR14 was 73.6 %. The polar lipids of NAR14 comprised one diphosphatidylglycerol, one phosphatidylethanolamine, one phosphatidylglycerol, one phosphatidylcholine, one aminolipid and three unknown polar lipids. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that NAR14 formed a lineage within the genus of the family , and it was distinct from the most closely related species JCM 19527 and JCM 17520 with the 16S rRNA gene sequence similarities of 94.61 and 93.98 %, respectively. Phenotypic characteristics (physiological, biochemical and chemotaxonomic) also supported the taxonomic novelty of this isolate. Thus, NAR14 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is NAR14 (=KCTC 92174 = MCCC 1K07275).
Topics: Animals; Fatty Acids; Phylogeny; RNA, Ribosomal, 16S; Anthozoa; Ubiquinone; DNA, Bacterial; Base Composition; Bacterial Typing Techniques; Sequence Analysis, DNA; Methylobacteriaceae; Phospholipids
PubMed: 36821368
DOI: 10.1099/ijsem.0.005745 -
International Journal of Systematic and... Nov 2020A Gram-stain-negative, aerobic, non-motile, pink-pigmented, coccus bacterium, designated CPCC 101081, was isolated from a gravel soil sample collected from Badain Jara...
A Gram-stain-negative, aerobic, non-motile, pink-pigmented, coccus bacterium, designated CPCC 101081, was isolated from a gravel soil sample collected from Badain Jara desert, PR China. Growth of the isolate occurred at 10-37 °C and pH 5.0-8.0, with optimal growth at 28-32 °C and pH 7.0, respectively. The major cellular fatty acids were summed feature 8 (Cω7/C ω6), summed feature 3 (C ω6/Cω7) and C2-OH. Q-10 was detected as the main respiratory quinone. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipid, an amino-containing lipid and an unidentified glycophospholipid were examined in the polar lipids extraction. The 16S rRNA gene sequence comparison of strain CPCC 101081 with the available sequences in the GenBank database showed that the isolate was closely related to members of the genus , with the highest similarity to DSM 14916 (97.4 %). In the phylogenetic trees based on 16S rRNA gene sequences and the core genomes, strain CPCC 101081 was included within the clade of the genus , representing a species level, with the closest neighbor of DSM 14916 . The genomic DNA G+C content was 68.7 mol%. The average nucleotide identity and the digital DNA-DNA hybridization values between strain CPCC 101081 and the related type strains of the genus were all far lower than the cut-off values for definition species. On the basis of above phenotypic and genotypic characteristics, strain CPCC 101081 is proposed to represent a novel species of the genus with the name sp. nov. strain CPCC 101081 (=KCTC 62852=NBRC 113512) is the type strain of the species.
Topics: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Desert Climate; Fatty Acids; Methylobacteriaceae; Nucleic Acid Hybridization; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Ubiquinone
PubMed: 32936752
DOI: 10.1099/ijsem.0.004467 -
International Journal of Systematic and... Oct 2021A novel species of the genus , designated SYSU M41301, was isolated from water sample of the Pearl River estuary in Guangdong, China. Polyphasic, taxonomic and...
A novel species of the genus , designated SYSU M41301, was isolated from water sample of the Pearl River estuary in Guangdong, China. Polyphasic, taxonomic and phylogenomic analyses were used to determine the taxonomy position of the strain. Phylogenetic analysis using 16S rRNA gene sequence indicated that strain SYSU M41301 showed the highest sequence similarity to KCTC 22213 (97.9 %) and KCTC 23339 (96.4 %). The novel species could be differentiated from other species of the genus by its distinct phenotypic and genotypic characteristics. The isolate was Gram-staining-negative, aerobic, short rod-shape, oxidase-positive and non-motile. The predominant respiratory quinone was ubiquinone 8 (Q-8). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, and one unidentified polar lipid. The major fatty acids (>10 % of total) were 11-methyl C 7, summed feature 3 (C c / or C c) and summed feature 8 (C 7 and/or C 6). The G+C content of the novel isolate based on genomic DNA was 72.0 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU M41301 should be considered to represent a novel species in the genus for which the name sp. nov. is proposed with the type strain SYSU M41301 (=KCTC 72726=CGMCC 1.18613).
Topics: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Estuaries; Fatty Acids; Methylobacteriaceae; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Rivers; Sequence Analysis, DNA; Ubiquinone
PubMed: 34623235
DOI: 10.1099/ijsem.0.004994 -
International Journal of Systematic and... Feb 2021A Gram-stain-negative, aerobic, mesophilic, non-motile bacterium, designated M0104, was isolated from a gorgonian coral collected from Xieyang island, Guangxi Province,...
A Gram-stain-negative, aerobic, mesophilic, non-motile bacterium, designated M0104, was isolated from a gorgonian coral collected from Xieyang island, Guangxi Province, PR China. Colonies of the strain were non-motile cocci and pink. The strain grew at 15-34 °C (optimum, 28 °C), pH 4.5-8.0 (optimum, pH 7.0) and with 0-4% (w/v) NaCl (optimum, 0-2 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0104 was closely related to JCM 31275 (96.2 %), KCTC 62043 (96.0 %), JCM 31878 (95.9 %) and KCTC 42542 (95.7 %). The strain had an assembly size of 5.0 Mb and a G+C content of 71.0mol%. Genes involved in copper, cadmium, lead, arsenic and zinc resistance were identified in the genome of strain M0104. The digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain M0104 and those of closely related type strains were 19.4-24.9 % and 74.3-81.8 %, respectively. Strain M0104 contained C 7, C 3, anteiso C and C as the major fatty acids (>7 %) and ubiquinone Q-10 as the sole isoprenoid quinone. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine were its major polar lipids. Based on its phenotypic, phylogenetic and chemotaxonomic properties, strain M0104 is proposed to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is M0104 (=KCTC 62359=MCCC 1K03632).
Topics: Animals; Anthozoa; Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Fatty Acids; Metals, Heavy; Methylobacteriaceae; Nucleic Acid Hybridization; Phospholipids; Phylogeny; Pigmentation; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Ubiquinone
PubMed: 33406032
DOI: 10.1099/ijsem.0.004624