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Infectious Disease Reports May 2020is a gram-positive organism found in food products as well as naturally occurring in air and on surfaces. We present the first known case of osteomyelitis caused by...
is a gram-positive organism found in food products as well as naturally occurring in air and on surfaces. We present the first known case of osteomyelitis caused by machine injection injury. The patient was treated with emergent surgical debridement as well as doxycycline for a soft tissue infection. Despite targeted therapy, the infection progressed to osteomyelitis and was treated successfully with additional surgical debridement and trimethoprim-sulfamethoxazole. There is sparse information on both infections and treatment of . We present our case report as well as a review of the literature on the epidemiology, susceptibility and treatment recommendations for infections.
PubMed: 32913620
DOI: 10.4081/idr.2020.8523 -
Journal of Chemical Ecology Jan 2022Mammals have microbes resident in their reproductive tract, some of which can be pathogenic while others may play a role in protecting the tract from infection. Volatile...
Mammals have microbes resident in their reproductive tract, some of which can be pathogenic while others may play a role in protecting the tract from infection. Volatile compounds play a role as sex pheromones that attract males for coitus during female estrus or heat. It is likely that these compounds themselves are secondary metabolites of bacterial flora resident in the vagina. In order to substantiate this hypothesis, bacteria were isolated from cervico-vaginal mucus (CVM) of buffalo during various phases of the estrous cycle and identified, using morphological, biochemical and molecular characteristics, as Bacillus during preestrus and diestrus, and as Staphylococcus during all three phases of the estrous cycle. Populations of Staphylococcus differed between different phases of the estrous cycle, the predominant forms being S. warneri (BCVMPE1_1) during preestrus, S. pastueri (BCVME2) during estrus and S. epidermis (BCVMDE3) during diestrus. Mice were used as chemosensors to differentiate the estrus-specific S. pasteuri (BCVME2) from the others. Chemical analysis showed that S. pasteuri (BCVME2) produced acetic, propanoic, isobutyric, butyric, isovaleric and valeric acids. In addition, it was shown that S. pasteuri (BCVME2) volatiles influenced the sexual behaviors, flehmen and mounting, of the bull. Thus, S. pasteuri (BCVME2) is a potential source of vaginal pheromone(s) during estrus in buffalo.
Topics: Animals; Buffaloes; Estrus; Female; Male; Mice; Mucus; Sex Attractants; Staphylococcus; Vagina
PubMed: 34542784
DOI: 10.1007/s10886-021-01311-9 -
IDCases 2019is a coagulase negative bacterium which although formally described in 1993, has only recently become possible to reliably speciate in diagnostic microbiology...
is a coagulase negative bacterium which although formally described in 1993, has only recently become possible to reliably speciate in diagnostic microbiology laboratories. remains an extremely infrequent cause of human infection to date, namely bacteremia in an individual suffering acute myeloid leukemia, catheter-associated urinary tract infection in a patient receiving chemotherapy and endocarditis within a case series without specific clinical information. As such, our report provides the first detailed account of infective endocarditis entailing a subacute community-onset infection involving native aortic and mitral valves, multiple systemic emboli, and ultimately cardiothoracic surgery.
PubMed: 31720224
DOI: 10.1016/j.idcr.2019.e00656 -
Biomolecules Mar 2022Bacterial extracellular proteins participate in the host cell communication by virtue of the modulation of pathogenicity, commensalism and mutualism. Studies on the...
Bacterial extracellular proteins participate in the host cell communication by virtue of the modulation of pathogenicity, commensalism and mutualism. Studies on the microbiome of cervical mucus of the water buffalo () have shown the occurrence of and that the presence of this bacterium is indicative of various physiological and reproductive states in the host. Recently, has been isolated from the cervical mucus of the buffalo during the different phases of estrous cycle, and has proved to be much more pronounced during the estrus phase. The basis underlying the availability of a significantly increased population, specifically during the estrus phase, is not known. Consequently, it is important to determine the significance of the specific abundance of during the estrus phase of the buffalo host, particularly from the perspective of whether this bacterial species is capable of contributing to sexual communication via its extracellular proteins and volatiles. Therefore, the relevance of exoproteome in the buffalo cervical mucus during the estrus phase was analyzed using LC-MS/MS. As many as 219 proteins were identified, among which elongation factor Tu (EF-Tu), 60-kDa chaperonin (Cpn60), enolase, fructose-bisphosphate aldolase class 1 (FBP aldolase), enoyl-[acyl-carrier-protein] reductase [NADPH] (ENR) and lipoprotein (Lpp) were the functionally important candidates. Most of the proteins present in the exoproteome of were those involved in cellular-metabolic functions, as well as catalytic- and binding activities. Moreover, computational studies of Lpp have shown enhanced interaction with volatiles such as acetic-, butanoic-, isovaleric- and valeric acids, which were identified in the cervical mucus . culture supernatant. The present findings suggest that extracellular proteins may play an important role in buffalo sexual communication during the estrus phase.
Topics: Animals; Buffaloes; Cervix Mucus; Chromatography, Liquid; Estrus; Female; Staphylococcus; Tandem Mass Spectrometry
PubMed: 35327642
DOI: 10.3390/biom12030450 -
Recent Patents on Anti-infective Drug... Jun 2009Staphylococcus pasteuri is a coagulase-negative, Gram positive organism which is emerging as an agent of nosocomial infections and a blood derivatives contaminant,... (Review)
Review
Staphylococcus pasteuri is a coagulase-negative, Gram positive organism which is emerging as an agent of nosocomial infections and a blood derivatives contaminant, though its role in causing human disease mostly remains controversial. Despite the paucity of isolates recovered, this bacterium has recently appeared to express resistance against several classes of antibiotic compounds, such as methicillin/oxacillin, macrolides, lincosamides, streptogramins, tetracyclines, chloramphenicol, streptomycin, fosfomycin, as well as quaternary ammonium compounds. Also, authors will discuss some essential patents related to the topic reviewed.
Topics: Animals; Anti-Bacterial Agents; Coagulase; Drug Resistance, Multiple, Bacterial; Humans; Lampreys; Mammals; Patents as Topic; Staphylococcal Infections; Staphylococcus
PubMed: 19519547
DOI: 10.2174/157489109788490352 -
Case Reports in Cardiology 2023A 45-year-old woman was admitted with severe pain in the right leg and dyspnea. Her medical history included previous endocarditis, biological aortic valve replacement,...
A 45-year-old woman was admitted with severe pain in the right leg and dyspnea. Her medical history included previous endocarditis, biological aortic valve replacement, and intravenous drug abuse. She was febrile but did not have any focal signs of infection. Blood tests showed raised infectious markers and troponin levels. Electrocardiogram showed sinus rhythm without signs of ischemia. Ultrasound revealed thrombosis of the right popliteal artery. The leg was not critically ischemic, and therefore, treatment with dalteparin was chosen. Transesophageal echocardiography showed an excrescence on the biological aortic valve. Empiric treatment for endocarditis was started with intravenous vancomycin, gentamicin, and oral rifampicin. Blood cultures subsequently grew . On day 2, treatment was changed to intravenous cloxacillin. Due to the comorbidity, the patient was not a candidate for the surgical treatment. On day 10, the patient developed moderate expressive aphasia and weakness in the right upper limb. Magnetic resonance imaging showed micro-embolic lesions scattered across both hemispheres of the brain. Treatment was changed from cloxacillin to cefuroxime. On day 42, infectious markers were normal, and echocardiography showed regression of the excrescence. Antibiotic treatment was stopped. Follow-up on day 52 did not show any signs of active infection. However, on day 143, the patient was readmitted with cardiogenic shock due to aortic root fistulation to the left atrium. She quickly deteriorated and died.
PubMed: 37013024
DOI: 10.1155/2023/4624492 -
Journal of Food Protection Nov 2018Staphylococcus aureus is an important pathogen implicated in various diseases, including staphylococcal food poisoning. Bacteriocins are considered safe and effective...
Staphylococcus aureus is an important pathogen implicated in various diseases, including staphylococcal food poisoning. Bacteriocins are considered safe and effective antimicrobial substances for the prevention of the growth of pathogenic bacteria. In this article, we describe the purification and characterization of pasteuricin, a novel bacteriocin produced by Staphylococcus pasteuri RSP-1. A cell-free supernatant of S. pasteuri RSP-1 exerted strong antimicrobial activity against staphylococci, including methicillin-resistant S. aureus (MRSA), and gram-positive bacteria. The loss of antimicrobial activity upon treatment with proteolytic enzymes confirmed the proteinaceous nature of pasteuricin. A rapid and pronounced bactericidal effect of pasteuricin was confirmed by a live-dead bacterial viability assay. To our knowledge, pasteuricin is the first reported S. pasteuri bacteriocin that inhibits S. aureus. Because pasteuricin is characterized by strong antimicrobial activity and high stability, it has potential as an alternative antimicrobial agent to antibiotics.
Topics: Anti-Bacterial Agents; Bacteriocins; Food Contamination; Food Preservation; Humans; Methicillin-Resistant Staphylococcus aureus; Staphylococcal Infections; Staphylococcus; Staphylococcus aureus
PubMed: 30280936
DOI: 10.4315/0362-028X.JFP-18-111 -
FEMS Microbiology Letters Oct 1995A rapid polymerase chain reaction method was developed to differentiate Staphylococcus pasteuri from other staphylococcal species, especially the phenotypically similar... (Comparative Study)
Comparative Study
A rapid polymerase chain reaction method was developed to differentiate Staphylococcus pasteuri from other staphylococcal species, especially the phenotypically similar S. warneri. The oligonucleotide probes used as primers were designed from the sequence of a S. pasteuri random amplified polymorphic DNA fragment.
Topics: Bacteriological Techniques; Base Sequence; DNA, Bacterial; Gene Amplification; Molecular Sequence Data; Oligonucleotide Probes; Phenotype; Phylogeny; Polymerase Chain Reaction; Polymorphism, Genetic; Sequence Analysis, DNA; Staphylococcus
PubMed: 7590156
DOI: 10.1111/j.1574-6968.1995.tb07824.x -
International Journal of Systematic... Apr 1993A new novobiocin-susceptible species of the genus Staphylococcus, Staphylococcus pasteuri, is described on the basis of the results of a study of seven strains isolated... (Comparative Study)
Comparative Study
A new novobiocin-susceptible species of the genus Staphylococcus, Staphylococcus pasteuri, is described on the basis of the results of a study of seven strains isolated from human, animal, and food specimens. DNA relatedness experiments (S1 nuclease method) showed that these strains form a homogeneous genomic species related at DNA homology levels of 2 to 13% to 27 type strains representing known Staphylococcus species. The use of a method based on rRNA gene restriction site polymorphism provides clear-cut distinction between this new species and Staphylococcus warneri, which is the most similar species phenotypically. The type strain of the new species is strain BM9357 (= ATCC 51129).
Topics: Amino Acids; Animals; Bacterial Typing Techniques; Cell Wall; DNA, Bacterial; DNA, Ribosomal; Food Microbiology; Humans; Microbial Sensitivity Tests; Novobiocin; Phenotype; Polymorphism, Restriction Fragment Length; Staphylococcus; Teichoic Acids
PubMed: 8098615
DOI: 10.1099/00207713-43-2-237 -
Journal of Global Antimicrobial... Jun 2018
Topics: Bacterial Proteins; Glycopeptides; Humans; Membrane Proteins; Microbial Sensitivity Tests; Mutation; Polymorphism, Single Nucleotide; Staphylococcal Infections; Staphylococcus
PubMed: 29660411
DOI: 10.1016/j.jgar.2018.04.002