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Acta Dermato-venereologica Aug 2018
Topics: Adolescent; Dermoscopy; Female; Humans; Molluscum Contagiosum; Molluscum contagiosum virus; Sacrococcygeal Region; Skin
PubMed: 29701237
DOI: 10.2340/00015555-2955 -
The Journal of Dermatology Oct 2018
Topics: Administration, Cutaneous; Combined Modality Therapy; Cryotherapy; DNA, Viral; Humans; Imiquimod; Male; Middle Aged; Molluscum Contagiosum; Molluscum contagiosum virus; Nitrogen; Penis; Skin; Treatment Outcome
PubMed: 29667708
DOI: 10.1111/1346-8138.14319 -
The Journal of General Virology Feb 2018Molluscum contagiosum virus (MCV) causes persistent, benign skin neoplasm in children and adults. MCV is refractive to growth in standard tissue culture and there is no... (Comparative Study)
Comparative Study
Molluscum contagiosum virus (MCV) causes persistent, benign skin neoplasm in children and adults. MCV is refractive to growth in standard tissue culture and there is no relevant animal model of infection. Here we investigated whether another poxvirus (vaccinia virus; VACV) could be used to examine MCV immunoevasion protein properties in vivo. The MCV MC159L or MC160L genes, which encode NF-κB antagonists, were inserted into an attenuated VACV lacking an NF-κB antagonist (vΔA49), creating vMC159 and vMC160. vMC160 slightly increased vΔA49 virulence in the intranasal and intradermal routes of inoculation. vMC159 infection was less virulent than vΔA49 in both inoculation routes. vMC159-infected ear pinnae did not form lesions, but virus replication still occurred. Thus, the lack of lesions was not due to abortive virus replication. This system provides a new approach to examine MCV immunoevasion proteins within the context of a complete and complex immune system.
Topics: Administration, Intranasal; Animals; Child; Female; Humans; Injections, Intradermal; Mice, Inbred BALB C; Molluscum contagiosum virus; NF-kappa B; Vaccinia virus; Viral Proteins; Virulence
PubMed: 29393023
DOI: 10.1099/jgv.0.001006 -
The British Journal of Dermatology Jul 2018
Topics: Case-Control Studies; Cell Cycle Proteins; Humans; Intracellular Signaling Peptides and Proteins; Molluscum Contagiosum; Molluscum contagiosum virus; Nuclear Proteins; Protein Serine-Threonine Kinases; Skin; Trans-Activators; Transcription Factors; Transcriptional Coactivator with PDZ-Binding Motif Proteins
PubMed: 29330849
DOI: 10.1111/bjd.16333 -
Journal of the American Academy of... Jun 2018
Topics: Eyelid Diseases; Follow-Up Studies; Humans; Keratomileusis, Laser In Situ; Molluscum Contagiosum; Molluscum contagiosum virus; Surgical Instruments; Treatment Outcome
PubMed: 29274346
DOI: 10.1016/j.jaad.2017.12.036 -
Autoinoculation as a treatment modality for molluscum contagiosum: A preliminary uncontrolled trial.Indian Journal of Dermatology,... 2018
Topics: Follow-Up Studies; Humans; Immunotherapy; Molluscum Contagiosum; Molluscum contagiosum virus; Prospective Studies; Treatment Outcome
PubMed: 29251278
DOI: 10.4103/ijdvl.IJDVL_1033_16 -
Current Protocols in Microbiology Nov 2017Molluscum contagiosum virus (MCV) is a common skin pathogen of children and young adults. Infection with MCV causes benign skin tumors in children and young adults and...
Molluscum contagiosum virus (MCV) is a common skin pathogen of children and young adults. Infection with MCV causes benign skin tumors in children and young adults and is mostly self-limiting. In contrast to orthopoxviruses, MCV infections tend to take a subacute clinical course but may persist for up to 12 months. Current numbers for MCV seroprevalence in different geographical areas are based on a variety of historical serological methods from complement fixation assays to MCV ELISAs based on purified MCV virions and MC133 antigen expressed in a Semliki Forest Virus expression system. A standardized ELISA for the assessment of MCV seroprevalence would be useful to determine global MCV seroprevalence. The methods described show that polypeptides derived from MCV open reading frames MC084 (residues V123 to R230 and V33 to G117), mc133 (residues M1 to N370), and glutathione S-transferase (GST)-H3L (residues I142 to W251) expressed in E. coli RIL+ as GST fusion proteins can be used to assess antibody binding in a GST capture ELISA. We show how the ELISA can be used to screen a panel of patient sera previously characterized with the mc084 V123-R230 ELISA. © 2017 by John Wiley & Sons, Inc.
Topics: Antibodies, Viral; Antigens, Viral; Enzyme-Linked Immunosorbent Assay; Humans; Molluscum Contagiosum; Molluscum contagiosum virus; Recombinant Proteins; Seroepidemiologic Studies
PubMed: 29120484
DOI: 10.1002/cpmc.42 -
International Journal of STD & AIDS Nov 2017
Topics: Adult; Dermoscopy; Hair Removal; Humans; Immunocompetence; Male; Middle Aged; Molluscum Contagiosum; Molluscum contagiosum virus
PubMed: 29027896
DOI: 10.1177/0956462417732650 -
Virus Research Oct 2017Poxviruses have previously been detected in macropods with cutaneous papillomatous lesions, however to date, no comprehensive analysis of a poxvirus from kangaroos has...
Poxviruses have previously been detected in macropods with cutaneous papillomatous lesions, however to date, no comprehensive analysis of a poxvirus from kangaroos has been performed. Here we report the genome sequences of a western grey kangaroo poxvirus (WKPV) and an eastern grey kangaroo poxvirus (EKPV), named for the host species from which they were isolated, western grey (Macropus fuliginosus) and eastern grey (Macropus giganteus) kangaroos. Poxvirus DNA from WKPV and EKPV was isolated and entire coding genome regions determined through Roche GS Junior and Illumina Miseq sequencing, respectively. Viral genomes were assembled using MIRA and SPAdes, and annotations performed using tools available from the Viral Bioinformatics Resource Centre. Histopathology and transmission electron microscopy analysis was also performed on WKPV and its associated lesions. The WKPV and EKPV genomes show 96% identity (nucleotide) to each other and phylogenetic analysis places them on a distinct branch between the established Molluscipoxvirus and Avipoxvirus genera. WKPV and EKPV are 170 kbp and 167 kbp long, containing 165 and 162 putative genes, respectively. Together, their genomes encode up to 47 novel unique hypothetical proteins, and possess virulence proteins including a major histocompatibility complex class II inhibitor, a semaphorin-like protein, a serpin, a 3-β-hydroxysteroid dehydrogenase/δ 5→4 isomerase, and a CD200-like protein. These viruses also encode a large putative protein (WKPV-WA-039 and EKPV-SC-038) with a C-terminal domain that is structurally similar to the C-terminal domain of a cullin, suggestive of a role in the control of host ubiquitination. The relationship of these viruses to members of the Molluscipoxvirus and Avipoxvirus genera is discussed in terms of sequence similarity, gene content and nucleotide composition. A novel genus within subfamily Chordopoxvirinae is proposed to accommodate these two poxvirus species from kangaroos; we suggest the name, Thylacopoxvirus (thylaco-: [Gr.] thylakos meaning sac or pouch).
Topics: Animals; Genome, Viral; Histocytochemistry; Macropodidae; Microscopy, Electron; Molecular Sequence Annotation; Phylogeny; Poxviridae; Poxviridae Infections; Sequence Analysis, DNA
PubMed: 28958947
DOI: 10.1016/j.virusres.2017.09.016 -
Clinical and Experimental Dermatology Dec 2017
Clinical Trial
Topics: Administration, Topical; Child; Child, Preschool; Female; Follow-Up Studies; Humans; Linoleic Acids; Male; Molluscum Contagiosum; Molluscum contagiosum virus; Oenothera biennis; Outcome Assessment, Health Care; Plant Oils; Skin; gamma-Linolenic Acid
PubMed: 28940438
DOI: 10.1111/ced.13226