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Animals : An Open Access Journal From... Mar 2022Performing Sectio Caesarea in sheep under general anaesthesia is a common procedure in veterinary practice. The abdominal cavity can be accessed via linea alba, for...
BACKGROUND
Performing Sectio Caesarea in sheep under general anaesthesia is a common procedure in veterinary practice. The abdominal cavity can be accessed via linea alba, for which the ewe is positioned in the supine position, whereby rumen and uterus can compromise lung function. Although the rumen represents an important reservoir for fluid and electrolytes, and kidney function during anaesthesia is essential, these parameters have not been focused on in research. Therefore, the objective of this study is to contribute data on blood parameters, ruminal fluid, and kidney function tests during laparotomy.
METHODS
Laparotomy was performed in 14 ewes, whereof five animals were pregnant ewes (PE) and nine non-pregnant ewes (NPE). A total of seven animals received isoflurane in addition to oxygen (inhalational anaesthesia (InhA)) and seven ewes were anaesthetised with xylazine and ketamine (total intravenous anaesthesia (TIVA)); all ewes received lumbosacral anaesthesia. Blood, urine, and ruminal fluid were sampled every hour over a three-hour period.
RESULTS
On comparing InhA to TIVA, higher values were detected for TIVA in haemoglobin, paced cell volume, sodium, phosphate, glucose concentration in the blood, and phosphate in ruminal fluid. Lower values were detected for TIVA in partial pressure of oxygen, oxygen saturation, and creatinine clearance. On comparing PE to NPE, higher values were detected in PE in magnesium and ruminal calcium. Lower values in PE were detected in chloride, base excess in the blood, and ruminal phosphate. Over time, an increase in partial pressure of carbon dioxide, glucose in the blood, glucose in urine, and a decrease in protein and albumin could be observed.
CONCLUSION
Surgery in sheep in the supine position should be performed with additional oxygen to maintain physiological pO and sO values. Kidney function could be maintained with a minimal electrolyte infusion regime. Additional glucose is not necessary, even in pregnant ewes. Further research should be conducted on parameters in ruminal fluid.
PubMed: 35405824
DOI: 10.3390/ani12070834 -
Animals : An Open Access Journal From... Mar 2022The gastrointestinal microbiota greatly affects the health status and production performance of bovines. Presently, many studies have used high-throughput sequencing...
The gastrointestinal microbiota greatly affects the health status and production performance of bovines. Presently, many studies have used high-throughput sequencing methods to investigate the gastrointestinal microbiome in bovines. However, the microbiome profile of crossbred cattle across the whole gastrointestinal tract (GIT) has not been thoroughly reported. In this study, the digesta at ten regions (including the rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon, and rectum) of the GIT were collected in three Simmental × Holstein crossbred heifers aged 17 months, and microbial DNA was extracted and amplified for sequencing of the V3-V4 regions of the 16S rRNA gene. Functional orthologs of the microbiota genome were predicted and analyzed. We found that samples were categorized into three groups (the stomach, small intestine, and large intestine) by principal coordinate analysis (PCoA) based on Bray-Curtis dissimilarity in both the bacterial composition and functional profile. Samples from small intestine had the lowest alpha diversity of bacteria composition and highest alpha diversity of the functional composition. Three groups of GIT regions were characterized by several microbiome features. The stomach was characterized by and at the phylum level, and KEGG pathways related to the metabolism of cofactors and vitamins, glycan biosynthesis, and metabolism were enriched in the stomach. The small intestine was characterized by and at the phylum level, and KEGG pathways related to xenobiotics biodegradation and metabolism were enriched in the small intestine. The large intestine featured , , and at the family level, and KEGG pathways, including steroid hormone biosynthesis, linoleic acid metabolism, and cysteine and methionine metabolism were enriched in the large intestine. The results of the current study revealed the spatial heterogeneity of microbiota across the GIT in Simmental × Holstein crossbreeds and identified microbial biomarkers of different regions. The results can provide useful information for the study of the gastrointestinal microbiome in bovines.
PubMed: 35405815
DOI: 10.3390/ani12070825 -
Frontiers in Microbiology 2022Myostatin (MSTN), a major negative regulator of skeletal muscle mass and an endocrine factor, can regulate the metabolism of various organisms. Inhibition of the gene...
Myostatin (MSTN), a major negative regulator of skeletal muscle mass and an endocrine factor, can regulate the metabolism of various organisms. Inhibition of the gene can improve meat production from livestock. Rumen microorganisms are associated with production and health traits of cattle, but changes in the microbial composition and metabolome in the four stomach compartments of gene-edited cattle have not previously been studied. Our results indicated that microbial diversity and dominant bacteria in the four stomach compartments were very similar between gene-edited and wild-type (WT) cattle. The microbiota composition was significantly different between gene-edited and WT cattle. Our results show that the relative abundance of the phylum Proteobacteria in the reticulum of gene-edited cattle was lower than that of WT cattle, whereas the relative abundance of the genus in the omasum of gene-edited cattle was significantly higher than that of WT cattle. Metabolomics analysis revealed that the intensity of L-proline and acetic acid was significantly different in the rumen, reticulum, and abomasum between the two types of cattle. Meanwhile, pathway topology analysis indicated that the differential metabolites were predominantly involved in arginine biosynthesis and glutamate metabolism in the rumen, reticulum, and omasum but were mainly involved in pyruvate metabolism and glycolysis/gluconeogenesis in the abomasum. Spearman correlation network analysis further demonstrated that there was a significant correlation between microflora composition and metabolic pathways. These findings provide clues for studying nutrient digestion and absorption ability of gene-edited cattle.
PubMed: 35401485
DOI: 10.3389/fmicb.2022.844962 -
Brazilian Journal of Microbiology :... Mar 2022Klebsiella variicola is generally known as endophyte as well as lignocellulose-degrading strain. However, their roles in goat omasum along with lignocellulolytic genetic...
Klebsiella variicola is generally known as endophyte as well as lignocellulose-degrading strain. However, their roles in goat omasum along with lignocellulolytic genetic repertoire are not yet explored. In this study, five different pectin-degrading bacteria were isolated from a healthy goat omasum. Among them, a new Klebsiella variicola strain HSTU-AAM51 was identified to degrade lignocellulose. The genome of the HSTU-AAM51 strain comprised 5,564,045 bp with a GC content of 57.2% and 5312 coding sequences. The comparison of housekeeping genes (16S rRNA, TonB, gyrase B, RecA) and whole-genome sequence (ANI, pangenome, synteny, DNA-DNA hybridization) revealed that the strain HSTU-AAM51 was clustered with Klebsiella variicola strains, but the HSTU-AAM51 strain was markedly deviated. It consisted of seventeen cellulases (GH1, GH3, GH4, GH5, GH13), fourteen beta-glucosidase (2GH3, 7GH4, 4GH1), two glucosidase, and one pullulanase genes. The strain secreted cellulase, pectinase, and xylanase, lignin peroxidase approximately 76-78 U/mL and 57-60 U/mL, respectively, when it was cultured on banana pseudostem for 96 h. The catalytically important residues of extracellular cellulase, xylanase, mannanase, pectinase, chitinase, and tannase proteins (validated 3D model) were bound to their specific ligands. Besides, genes involved in the benzoate and phenylacetate catabolic pathways as well as laccase and DiP-type peroxidase were annotated, which indicated the strain lignin-degrading potentiality. This study revealed a new K. variicola bacterium from goat omasum which harbored lignin and cellulolytic enzymes that could be utilized for the production of bioethanol from lignocelluloses.
Topics: Animals; Goats; Klebsiella; Omasum; Phylogeny; RNA, Ribosomal, 16S
PubMed: 35088248
DOI: 10.1007/s42770-021-00660-7 -
Journal of Dairy Science Mar 2022The digestive tract development in goat kids around weaning is vital to the establishment of digestion and absorption function, growth, and health of adults. The...
The digestive tract development in goat kids around weaning is vital to the establishment of digestion and absorption function, growth, and health of adults. The objective was to explore the effects of age and solid feed on the anatomical and morphological development of the gastrointestinal tract of Laiwu Black goat kids. Forty-eight female Laiwu Black goats at 8 ages (1, 7, 14, 28, 42, 56, 70, and 84 d; 6 goats per group) were selected and killed for anatomical and morphological analysis. The goats experienced the following 4 diet phases: maternal colostrum (MC; d 1, d 7), maternal milk (MM; d 14, d 28), maternal milk plus solid diet (MMSD; d 42, d 56) and only solid diet (OSD; d 70, d 84). The body and carcass weights were not significantly changed during MC and MM phases but changed during the MMSD phase. The absolute growth of body and carcass weights were higher in the MMSD phase than in MM phase. In addition, the dressing percentage was the highest in the MMSD phase. The body size indices evolved progressively and increased over time. The percentage of internal and external organs to body weight decreased over time, whereas the percentage to complex stomach percentage increased. The rumen and omasum weight experienced synchronous absolute growth over time, especially in the OSD phase. In contrast, the absolute growth of the reticulum and abomasum was the highest in MMSD and MC phases, respectively. After weaning, the goats showed the highest papillae height, lamina propria, muscle layer thickness, and epithelial thickness. The OSD phase showed the highest colonic mucosa thickness, ileal villus height, and ileal muscle layer thickness. The crypt depth was higher in the MMSD phase than in the MM phase. Moreover, the crypt depth and muscle layer thickness of jejunum increased over time. Furthermore, duodenal crypt depth, muscle layer thickness, and epithelial thickness increased in the OSD phase compared with other stages. In conclusion, the histological investigation supports the improvement of the morphological development of the digestive tract and the growth performance in the solid feed phase. It is recommended to add solid food as early as 4 wk old.
Topics: Animal Feed; Animals; Colostrum; Diet; Female; Gastrointestinal Tract; Goats; Milk; Pregnancy; Rumen; Weaning
PubMed: 35086701
DOI: 10.3168/jds.2021-21056 -
Animal Microbiome Jan 2022Targeted modification of the dairy calf ruminal microbiome has been attempted through rumen fluid inoculation to alter productive phenotypes later in life. However,...
BACKGROUND
Targeted modification of the dairy calf ruminal microbiome has been attempted through rumen fluid inoculation to alter productive phenotypes later in life. However, sustainable effects of the early life interventions have not been well studied, particularly on the metabolically active rumen microbiota and its functions. This study investigated the sustained effects of adult-derived rumen fluid inoculations in pre-weaning dairy calves on the active ruminal microbiome of post-weaned dairy calves analyzed via RNA-sequencing.
RESULTS
Two different adult-derived microbial inocula (bacterial- or protozoal-enriched rumen fluid; BE or PE, respectively) were administered in pre-weaned calves (3-6 weeks) followed by analyzing active rumen microbiome of post-weaned calves (9 weeks). The shared bacterial community at the genus level of 16S amplicon-seq and RNA-seq datasets was significantly different (P = 0.024), 21 out of 31 shared major bacterial genera differed in their relative abundance between the two analytic pipelines. No significant differences were found in any of the prokaryotic alpha- and beta-diversity measurements (P > 0.05), except the archaeota that differed for BE based on the Bray-Curtis dissimilarity matrix (P = 0.009). Even though the relative abundances of potentially transferred microbial and functional features from the inocula were minor, differentially abundant prokaryotic genera significantly correlated to various fermentation and animal measurements including butyrate proportion, body weight, and papillae length and counts. The overall microbial functions were affected quantitatively by BE and qualitatively by PE (P < 0.05), and this might be supported by the individual KEGG module and CAZymes profile differences. Exclusive networks between major active microbial (bacterial and archaeal genera) and functional features (KEGG modules) were determined which were differed by microbial inoculations.
CONCLUSIONS
This study demonstrated that actively transcribed microbial and functional features showed reliable connections with different fermentations and animal development responses through adult rumen fluid inoculations compared to our previous 16S amplicon sequencing results. Exclusive microbial and functional networks of the active rumen microbiome of dairy calves created by BE and PE might also be responsible for the different ruminal and animal characteristics. Further understanding of the other parts of the gastrointestinal tract (e.g., abomasum, omasum, and small intestine) using metatranscriptomics will be necessary to elucidate undetermined biological factors affected by microbial inoculations.
PubMed: 34983694
DOI: 10.1186/s42523-021-00142-z -
Animal Nutrition (Zhongguo Xu Mu Shou... Dec 2021The unique structure of the stomach, including the rumen, reticulum, omasum, and abomasum, indicates the differences between the ruminant and monogastric animals in the... (Review)
Review
The unique structure of the stomach, including the rumen, reticulum, omasum, and abomasum, indicates the differences between the ruminant and monogastric animals in the digestion of nutrients. This difference is reflected in the majority of dietary nutrients that may be fermented in the rumen. Significant proteins and a certain amount of starch can flow to the small intestine apart from rumen. The initial phase of small intestinal digestion requires pancreatic digestive enzymes. In theory, the enzymatic digestion and utilization efficiency of starch in the small intestine are considerably higher than that in the rumen, but the starch digestibility in the small intestine is quite low in ruminants. Therefore, improving the digestion of nutrients, especially starch in the small intestine is more urgent for high-yield ruminants. Although the pancreas plays a central role in nutrient digestion, the progress of research investigating pancreatic exocrine regulation in the ruminant is slow due to some factors, such as the complex structure of the pancreas, the selection of experimental model and duration, and internal (hormones or ages) and external (diet) influences. The present review is based on the research findings of pancreatic exocrine regulation of dairy animals and expounded from the physiological structure of the ruminant pancreas, the factors affecting the digestion and exocrine processing of carbohydrates, and the regulatory mechanism governing this process. The review aims to better understand the characteristics of enzymatic digestion, thereby advancing pancreatic exocrine research and improving the digestion and utilization of nutrients in ruminants. Additionally, this review provides the theoretical basis for improving nutrient utilization efficiency, reducing wastage of feed resources, and promoting the efficient development of the dairy industry.
PubMed: 34754956
DOI: 10.1016/j.aninu.2021.09.004 -
PloS One 2021The purpose of this study was to investigate the effects of oscillating crude protein (CP) concentration diet on the nitrogen utilization efficiency (NUE) of calves and...
The purpose of this study was to investigate the effects of oscillating crude protein (CP) concentration diet on the nitrogen utilization efficiency (NUE) of calves and determine its mechanism. Twelve Holstein calves were assigned randomly into static protein diet (SP, 149 g/kg CP) and oscillating protein diet (OP, 125 and 173 g/kg CP diets oscillated at 2-d intervals) groups. After 60 days of feeding, the weights of total stomach, rumen and omasum tended to increase in calves fed OP. The apparent crude fat digestibility, NUE and energy metabolism also increased. In terms of urea-N kinetics evaluated by urea-15N15N isotope labeling method, the urea-N production and that entry to gastrointestinal tended to increase, and urea-N reused for anabolism increased significantly in calves fed OP during the low protein phase. These data indicate that urea-N recycling contributed to improving NUE when dietary protein concentration was low. In addition, the differentially expressed genes in rumen epithelium and the rumen bacteria involved in protein and energy metabolism promoted the utilization of dietary protein in calves fed OP.
Topics: Animal Feed; Animals; Bacteria; Biodiversity; Cattle; Diet; Dietary Proteins; Epithelium; Gene Expression Profiling; Kinetics; Liver; Male; Metabolomics; Nitrogen; Oscillometry; RNA, Ribosomal, 16S; Rumen; Transcriptome; Urea
PubMed: 34506606
DOI: 10.1371/journal.pone.0257417 -
Schweizer Archiv Fur Tierheilkunde Jun 2021This study was aimed to determine the effect of advanced pregnancy on the topography and size of the omasum in 22 healthy Murrah buffaloes. The omasum was scanned 15-20...
This study was aimed to determine the effect of advanced pregnancy on the topography and size of the omasum in 22 healthy Murrah buffaloes. The omasum was scanned 15-20 days before and after parturition, as per the standard procedure. The dorsal and ventral margins of the omasum were identified and marked at each intercostal space (ICS). The dorsal and ventral limits up to the dorsal midline were measured. The omasum was scanned in 6th to 11th ICS during advanced pregnancy and 7th to 11th ICS after the parturition. Irrespective of the pregnancy, the dorsal and ventral margins of the omasum were located farther dorsal and close to the spine in the 6th, 7th and 11th ICS. Except in one buffalo, the omasum was scanned in four consecutive ICS during the advanced pregnancy. After parturition the omasum was scanned in four and five consecutive ICS in 17 and five buffaloes, respectively. The mean dorsal and ventral limits of the omasum increased significantly (P .
Topics: Animals; Buffaloes; Female; Lactation; Omasum; Parturition; Pregnancy; Ultrasonography
PubMed: 34097635
DOI: 10.17236/sat00305 -
Animals : An Open Access Journal From... Apr 2021The reticulum and rumen are considered a single functional unit (the reticulo-rumen) with regards to myoelectrical and contractile activities. The specialized...
The reticulum and rumen are considered a single functional unit (the reticulo-rumen) with regards to myoelectrical and contractile activities. The specialized contractions of the reticulo-rumen provide constant mixing of partially digested material (cycle A), its flow into the omasum during eructation (cycle B), and regurgitation-rumination (cycle C). This study aimed to investigate the feasibility of electromyography (EMG) registered by a long-term telemetry method for assessment of the basic reticulo-rumen myoelectrical activity in sheep, to develop the effective recognition of the reticulo-rumen cycles at rest with no food stimulation, and to investigate the relationship between cycles A, B, and C in such basic conditions. The experiment was carried out on nine ewes. Myoelectric activity of the rumen, reticulum, and abomasum was recorded by the combination of three silver bipolar electrodes and a 3-channel transmitter implant. The myoelectrical activity registered successfully in the reticulum and rumen was determined as three characteristic patterns of cycles A, B, and C. The percentage of each type of cycle changed at different intervals from equally cycles A (43-50%) and B (50-56%), occurring when cycle C was not observed to the domination of cycle C (57-73%) with a decrease of cycles A (6-14%) and B (20-28%). The long-term EMG telemetry registration is feasible in the assessment of the reticulo-rumen myoelectrical activity in sheep.
PubMed: 33917991
DOI: 10.3390/ani11041052