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Research (Washington, D.C.) 2023Newborn ruminants are considered functionally monogastric animals. The poor understanding of cellular differences between newborn and mature ruminants prevents the...
Newborn ruminants are considered functionally monogastric animals. The poor understanding of cellular differences between newborn and mature ruminants prevents the improvement of health and performance of domestic ruminants. Here, we performed the single-cell RNA sequencing on the rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon, rectum, liver, salivary gland, and mammary gland from newborn and adult cattle. A comprehensive single-cell transcriptomic atlas covering 235,941 high-quality single cells and 78 cell types was deciphered. A Cattle Cell Landscape database (http://cattlecelllandscape.zju.edu.cn) was established to elaborately display the data and facilitate effective annotation of cattle cell types and subtypes for the broad research community. By measuring stemness states of epithelial cells in each tissue type, we revealed that the epithelial cells from newborn forestomach (rumen, reticulum, and omasum) were more transcriptionally indistinct and stochastic compared with the adult stage, which was in contrast to those of abomasum and intestinal tissues. The rapid forestomach development during the early life of calves was driven by epithelial progenitor-like cells with high DNA repair activities and methylation. Moreover, in the forestomach tissues of newborn calves, the genus was involved in regulating the transcriptional plasticity of the epithelial progenitor-like cells by DNA methylation regulation. A novel cell type, the cell, was found to be newborn-specific. It apparently plays a crucial role in stemness maintenance of its own and cholangiocytes in the hepatic microenvironment. Our results reveal that the age- and microbiota-dependent cell stemness plasticity drives the postnatal functional maturity of ruminants.
PubMed: 37040481
DOI: 10.34133/research.0025 -
Journal of Dairy Science Jun 2018Promotion of microbial butyrate production in the reticulorumen is a widely used method for enhancing forestomach development in calves. Additional acceleration of... (Review)
Review
Promotion of microbial butyrate production in the reticulorumen is a widely used method for enhancing forestomach development in calves. Additional acceleration of gastrointestinal tract (GIT) development, both the forestomach and lower parts of the GIT (e.g., abomasum, intestine, and also pancreas), can be obtained by dietary butyrate supplementation. For this purpose, different sources (e.g., butyrate salts or butyrins), forms (e.g., protected or unprotected), methods (e.g., in liquid feed or solid feed), and periods (e.g., before or after weaning) of butyrate administration can be used. The aim of this paper was to summarize the knowledge in the field of butyrate supplementation in feeds for newborn calves in practical situations, and to suggest directions of future studies. It has been repeatedly shown that supplementation of unprotected salts of butyrate (primarily sodium salt) in milk replacer (MR) stimulates the rumen, small intestine, and pancreas development in calves, with a supplementation level equating to 0.3% of dry matter being sufficient to exert the desired effect on both GIT development and growth performance. On the other hand, the effect of unprotected butyrins and protected forms of butyrate supplementation in MR has not been extensively investigated, and few studies have documented the effect of butyrate addition into whole milk (WM), with those available focusing mainly on the growth performance of animals. Protected butyrate supplementation at a low level (0.3% of protected product in DM) in solid feed was shown to have a potential to enhance GIT development and performance of calves fed MR during the preweaning period. Justification of this form of butyrate supplementation in solid feed when calves are fed WM or after weaning needs to be documented. After weaning, inclusion of unprotected butyrate salts in solid feed was shown to increase solid feed intake, but the effect on GIT development and function has not been determined in detail, and optimal levels of supplementation are also difficult to recommend based on available reports. Future studies should focus on comparing different sources (e.g., salts vs. esters), forms (e.g., protected vs. unprotected), and doses of supplemental butyrate in liquid feeds and solid feeds and their effect not only on the development of rumen, abomasum, and small intestine but also the omasum and large intestine. Furthermore, the most effective source, form, and dose of supplemental butyrate in solid feed depending on the liquid feed program (e.g., MR or WM), stage of rearing (e.g., pre- or postweaning), and solid composition (e.g., lack or presence of forage in the diet) need to be determined.
Topics: Animal Feed; Animals; Butyric Acid; Cattle; Diet; Dietary Supplements; Gastrointestinal Tract
PubMed: 29525310
DOI: 10.3168/jds.2017-14086 -
Journal of Dairy Science Jun 2018Many early studies laid the foundation for our understanding of the mechanics of chewing, the physiological role of chewing for the cow, and how chewing behavior is... (Review)
Review
Many early studies laid the foundation for our understanding of the mechanics of chewing, the physiological role of chewing for the cow, and how chewing behavior is affected by dietary characteristics. However, the dairy cow has changed significantly over the past decades, as have the types of diets fed and the production systems used. The plethora of literature published in recent years provides new insights on eating and ruminating activity of dairy cows. Lactating dairy cows spend about 4.5 h/d eating (range: 2.4-8.5 h/d) and 7 h/d ruminating (range: 2.5-10.5 h/d), with a maximum total chewing time of 16 h/d. Chewing time is affected by many factors, most importantly whether access to feed is restricted, intake of neutral detergent fiber from forages, and mean particle size of the diet. Feed restriction and long particles (≥19 mm) have a greater effect on eating time, whereas intake of forage neutral detergent fiber and medium particles (4-19 mm) affects rumination time. It is well entrenched in the literature that promoting chewing increases salivary secretion of dairy cows, which helps reduce the risk of acidosis. However, the net effect of a change in chewing time on rumen buffing is likely rather small; therefore, acidosis prevention strategies need to be broad. Damage to plant tissues during mastication creates sites that provide access to fungi, adhesion of bacteria, and formation of biofilms that progressively degrade carbohydrates. Rumination and eating are the main ways in which feed is reduced in particle size. Contractions of the rumen increase during eating and ruminating activity and help move small particles to the escapable pool and into the omasum. Use of recently developed low-cost sensors that monitor chewing activity of dairy cows in commercial facilities can provide information that is helpful in management decisions, especially when combined with other criteria. Although accuracy and precision can be somewhat variable depending on sensor and conditions of use, relative changes in cow behavior, such as a marked decrease in rumination time of a cow or sustained low rumination time compared with a contemporary group of cows, can be used to help detect estrus, parturition, and some illnesses. This review provides a comprehensive understanding of the dietary, animal, and management factors that affect eating and ruminating behavior in dairy cows and presents an overview of the physiological importance of chewing with emphasis on recent developments and practical implications for feeding and managing the modern housed dairy cow.
Topics: Animal Feed; Animals; Cattle; Digestion; Eating; Female; Lactation; Rumen
PubMed: 29627250
DOI: 10.3168/jds.2017-13706 -
Frontiers in Veterinary Science 2023The development of the four stomachs of yak is closely related to its health and performance, however the underlying molecular mechanisms are largely unknown. Here, we...
The development of the four stomachs of yak is closely related to its health and performance, however the underlying molecular mechanisms are largely unknown. Here, we systematically analyzed mRNAs of four stomachs in five growth time points [0 day, 20 days, 60 days, 15 months and 3 years (adult)] of yaks. Overall, the expression patterns of DEmRNAs were unique at 0 d, similar at 20 d and 60 d, and similar at 15 m and adult in four stomachs. The expression pattern in abomasum was markedly different from that in rumen, reticulum and omasum. Short Time-series Expression Miner (STEM) analysis demonstrated that multi-model spectra are drastically enriched over time in four stomachs. All the identified mRNAs in rumen, reticulum, omasum and abomasum were classified into 6, 4, 7, and 5 cluster profiles, respectively. Modules 9, 38, and 41 were the most significant three colored modules. By weighted gene co-expression network analysis (WGCNA), a total of 5,486 genes were categorized into 10 modules. , , , and were the hub genes of the turquoise module, and , , , , and were the hub genes of the blue module. Furthermore, functional KEGG enrichment analysis suggested that the turquoise module was involved in gastric acid secretion, sphingolipid metabolism, ether lipid metabolism, etc., and the blue module was enriched in pancreatic secretion, pantothenate and CoA biosynthesis, and starch and sucrose metabolism, etc. Our study aims to lay a molecular basis for the study of the physiological functions of rumen, reticulum, omasum and abomasum in yaks. It can further elucidate the important roles of these mRNAs in regulation of growth, development and metabolism in yaks, and to provide a theoretical basis for age-appropriate weaning and supplementary feeding in yaks.
PubMed: 37808112
DOI: 10.3389/fvets.2023.1204706 -
Journal of Applied Genetics Aug 2015The digestive systems of mammals harbor a complex gut microbiome, comprising bacteria and other microorganisms that confer metabolic and immunological benefits to the...
The digestive systems of mammals harbor a complex gut microbiome, comprising bacteria and other microorganisms that confer metabolic and immunological benefits to the host. Ruminants that digest plant-based foods have a four-compartment stomach consisting of the rumen, reticulum, omasum, and abomasum. The microorganisms in the stomach are essential for providing the host with critical nutrients. However, the majority of these microorganisms are unknown species. The microbiome of the stomach is diverse, and the majority of these organisms cannot be cultured. Next-generation sequencing (NGS) combined with bioinformatic analysis tools have allowed the dissection of the composition of the microbiome in samples collected from a specific environment. In this study, for the first time, the bacterial composition in two compartments, the reticulum and the omasum, of bovine were analyzed using a metagenomic approach and compared to the bacterial composition of the rumen. These data will assist in understanding the biology of ruminants and benefit the agricultural industry. The diversity and composition of the bacterial community in samples collected from the rumen, reticulum, and omasum of bovines in the Changchun Region of Northeast China were analyzed by sequencing the V3 region of the 16S rRNA gene using a barcoded Illumina paired-end sequencing technique, and the primary composition of the microbiome in the rumen, reticulum, and omasum of the bovines was determined. These microbiomes contained 17 phyla and 107 genera in all three samples. Five phyla, Bacteroidetes, Firmicutes, Proteobacteria, Spirochaetes, and Lentisphaerae, were the most abundant taxonomic groups. Additionally, the different stomach compartments harbored different compositions of the microorganisms.
Topics: Animals; Bacteria; Cattle; DNA, Bacterial; High-Throughput Nucleotide Sequencing; Metagenome; Metagenomics; Omasum; RNA, Ribosomal, 16S; Reticulum; Rumen; Sequence Analysis, DNA
PubMed: 25604266
DOI: 10.1007/s13353-014-0258-1 -
Schweizer Archiv Fur Tierheilkunde Jul 2012The reticulum, rumen, omasum, and abomasum were assessed via ultrasonography before, during, and 15, 30, and 120 minutes after feeding milk to 10 healthy calves. The...
The reticulum, rumen, omasum, and abomasum were assessed via ultrasonography before, during, and 15, 30, and 120 minutes after feeding milk to 10 healthy calves. The ultrasonographic examinations were conducted using a 5.0 MHz linear transducer. Loops were recorded on video for further evaluation. The reticulum could be visualised before feeding in seven calves. Its appearance and pattern of contractions were similar to those in adult cattle, although the amplitude (1.7 ± 0.75 cm) and velocity (2.7 ± 1.34 cm/s) of the first contraction were smaller than in adult cattle. The reticulum could not be visualised in any of the calves during feeding as it was displaced cranially and laterally and therefore being obscured by the lungs as the abomasum expanded with the ingested milk. 2 hours post ingestion it remained obscured in 5 individual and was visualized again the other 5. The position and size of the entire rumen including the dorsal and ventral sacs and the ruminal contents were assessed. There were no changes in the ultrasonographic appearance of the rumen during or after feeding. Except for its smaller size, the ultrasonographic appearance of the omasum of calves was similar to that of adult cattle. Milk flow through the omasum could not be seen in any of the calves, and there were no changes in the appearance of the omasum during and after feeding. The abomasum was seen to the left and right of the ventral midline before feeding in all calves; it occupied considerably more space on the left than the right. The flow of milk into the abomasum and milk clotting, which occurred 15 minutes after feeding, could be seen in all calves. The milk clots started to slowly disintegrate 30 minutes after the start of feeding, and by 2 hours post feeding, this process was greatly advanced but remained incomplete. Ultrasonography is an ideal tool for the evaluation of the reticulum, rumen, omasum, and abomasum before, during, and after the ingestion of milk in calves.
Topics: Animals; Blood Chemical Analysis; Blood Gas Analysis; Cattle; Female; Gastrointestinal Contents; Male; Milk; Stomach, Ruminant; Ultrasonography
PubMed: 22753252
DOI: 10.1024/0036-7281/a000348 -
Schweizer Archiv Fur Tierheilkunde Mar 2013This review article summarizes the ultrasonographic findings of the reticulum, rumen, omasum, abomasum and intestines of goats. Ultrasonographic examination is done on... (Review)
Review
This review article summarizes the ultrasonographic findings of the reticulum, rumen, omasum, abomasum and intestines of goats. Ultrasonographic examination is done on both sides with the goat in a standing position using a linear array or convex transducer with a frequency of 5.0 to 7.5 MHz. The shape, contour and motility of the reticulum are assessed; this organ appears as a crescent-shaped structure with a smooth contour immediately adjacent to the diaphragm. There are 0.8 to 2.1 reticular contractions per minute, which may be mono-, bi- and triphasic. The rumen is examined from the 8th to 12th intercostal spaces (ICSs) and flank on the left, and from the 12th ICS and flank on the right. The ruminal wall appears as a thick echoic line. The dorsal and ventral sacs of the rumen are differentiated based on the longitudinal groove, which forms an echoic notch. Differentiation of the dorsal gas cap, fibre mat and fluid layer is not always straightforward and varies among goats. The omasum is examined from the 6th to 11th ICSs on the right. Only the wall closest to the transducer can be imaged and it appears as a crescent-shaped echoic line medial to the liver. The omasal folds and the wall furthest from the transducer cannot be seen. In about two thirds of goats, active omasal motility is apparent as a transient reduction in size of several centimetres, followed by relaxation and return to its original size. There is an average of 1.1 contractions per minute. The abomasum is examined from the ventral midline and the left and right paramedian regions and can always be seen from the ventral midline. In all but a few goats the abomasum is also visible from the left and right paramedian areas. It appears as a heterogeneous, moderately echoic structure with echogenic stippling. The abomasal folds appear as prominent echoic bands in about two thirds of all goats. The small and large intestines are examined on the right from the 8th to the 12th ICSs. Loops of jejunum and ileum are seen mainly in cross-section and have a strong motility. The intestinal content is usually homogeneous and echoic and the diameter of the intestinal loops varies from 0.8 and 2.7 cm. The spiral colon and in many cases also the caecum can be imaged. The former is recognized by its garland-like appearance brought about by the centripetal and centrifugal coils of the intestine. In the spiral colon and the caecum, only the wall closest to the transducer can be imaged because of intraluminal gas. The wall appears as a thick echoic and slightly undulating line 5.6 to 8.0 cm in length. Ultrasonography is an imaging technique that is very well suited for the examination of the gastrointestinal tract of goats.
Topics: Abomasum; Animals; Gastrointestinal Motility; Goat Diseases; Goats; Intestinal Diseases; Intestines; Omasum; Reticulum; Rumen; Stomach Diseases; Stomach, Ruminant; Transducers; Ultrasonography
PubMed: 23454503
DOI: 10.1024/0036-7281/a000441 -
Veterinary Medicine International 2011Omasal impaction is a serious disease problem in cattle in India, but it is difficult to diagnose clinically. Ultrasonography has been proposed for the noninvasive...
Omasal impaction is a serious disease problem in cattle in India, but it is difficult to diagnose clinically. Ultrasonography has been proposed for the noninvasive evaluation of omasal disease. The objectives of this study were to compare the in vitro and in vivo ultrasonographic appearance of the omasum and to compare omasal appearance, limits, and size in clinically healthy cows with those in cows having confirmed omasal impaction. A 3.5 MHz curvilinear transducer was used to image and record the appearance of the omasum in vitro in a water bath, and its appearance, dorsal and ventral limits, and size in 10 healthy Indian Jersey/Red Sindhi crossbred cows. The results were compared with the ultrasonographic data collected from 5 cows with omasal impaction, as confirmed at necropsy. On moving the transducer dorsoventrally in each intercostal space and below the costal arch, the wall of omasum could be seen as an echogenic arc-like structure. The difference between mean dorsoventral extents of the normal and impacted omasums was statistically insignificant. These results suggest that ultrasonographic imaging may not be useful in the diagnosis of omasal impaction in Indian crossbred cows, however, additional studies may be warranted.
PubMed: 21647337
DOI: 10.4061/2011/485031 -
PloS One 2022Despite the growing interest in the ruminants' gastrointestinal tract (GIT) microbiomes' ability to degrade plant materials by animal husbandry and industrial sectors,...
Despite the growing interest in the ruminants' gastrointestinal tract (GIT) microbiomes' ability to degrade plant materials by animal husbandry and industrial sectors, only a few studies addressed browsing ruminants. The present work describes the taxonomic and functional profile of the bacterial and archaeal communities from five different gastrointestinal sections (rumen, omasum-abomasum, jejunum, cecum and colon) of browsing Capra hircus, by metabarcoding using 16S rRNA genes hypervariable regions. The bacterial communities across the GITs are mainly composed of Bacillota and Bacteroidota. Prevotella was the leading bacterial group found in the stomachs, Romboutsia in the jejuna, and Rikenellaceae_RC9_gut_group, Bacteroides, UCG-010_ge, UCG-005, and Alistipes in large intestines. The archaeal communities in the stomachs and jejuna revealed to be mainly composed of Methanobrevibacter, while in the large intestines its dominance is shared with Methanocorpusculum. Across the GITs, the main metabolic functions were related to carbohydrate, amino acid, and energy metabolisms. Significant differences in the composition and potential biological functions of the bacterial communities were observed among stomachs, jejuna and large intestines. In contrast, significant differences were observed among stomachs and jejuna verse large intestines for archaeal communities. Overall different regions of the GIT are occupied by different microbial communities performing distinct biological functions. A high variety of glycoside hydrolases (GHs) indispensable for degrading plant cell wall materials were predicted to be present in all the GIT sections.
Topics: Amino Acids; Animals; Archaea; Bacteria; Bacteroidetes; Carbohydrates; Gastrointestinal Microbiome; Glycoside Hydrolases; Goats; RNA, Ribosomal, 16S; Rumen
PubMed: 36251671
DOI: 10.1371/journal.pone.0276262 -
American Journal of Veterinary Research Jun 2008To describe ultrasonographic appearance of the liver, small and large intestines, and omasum in cows with right displacement of the abomasum (RDA) and with abomasal...
OBJECTIVE
To describe ultrasonographic appearance of the liver, small and large intestines, and omasum in cows with right displacement of the abomasum (RDA) and with abomasal volvulus (AV) and to determine whether RDA and AV can be differentiated on the basis of ultrasonographic findings.
ANIMALS
17 cows with RDA, 9 cows with AV, and 10 healthy control cows.
PROCEDURES
A linear transducer was used to examine the abomasum, liver, omasum, and small and large intestines from the right side. Results-The liver was imaged less frequently in cows with RDA or AV, compared with control cows. In 9 cows with RDA or AV, the liver could not be imaged. The small intestine was imaged less frequently in cows with RDA or AV than in control cows; in cows with AV, the small intestine could not be imaged in the 8th, 9th, or 10th intercostal space. The large intestine was imaged less frequently in the 11th and 12th intercostal spaces and the cranial region of the flank in cows with RDA or AV. The omasum was also imaged less frequently in the 8th and 9th intercostal spaces in cows with RDA or AV. Cows with RDA or AV could not be differentiated on the basis of ultrasonographic findings.
CONCLUSIONS AND CLINICAL RELEVANCE
Compared with control cows, cows with RDA and AV had changes in positioning and therefore extent of ultrasonographic imaging of the liver, omasum, and small and large intestines; however, these findings were not useful in differentiating between cows with RDA and AV.
Topics: Animals; Cattle; Cattle Diseases; Female; Intestine, Large; Intestine, Small; Liver; Omasum; Stomach Volvulus; Ultrasonography
PubMed: 18518658
DOI: 10.2460/ajvr.69.6.777