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Orthopadie (Heidelberg, Germany) May 2024The use of allografts and autografts has been met with mixed views on whether allografts are a suitable alternative to autografts.
Peracetic acid sterilized tendon and ligament allografts for knee reconstruction : For anterior cruciate ligament (ACL), posterior cruciate ligament (PCL) and complex knee surgery.
BACKGROUND
The use of allografts and autografts has been met with mixed views on whether allografts are a suitable alternative to autografts.
QUESTION
We aimed to investigate if chemically sterilized allografts show similar rerupture rates to those reported in the literature for allografts and autografts in anterior (ACL) and posterior cruciate ligaments (PCL) and complex knee surgery.
MATERIALS AND METHODS
Retrospective data on knee reconstructions performed between 2011 and 2015 with tendon/ligamnet allografts sterilized with peracetic acid were collected in the form of a questionnaire. The inclusion criteria of 2 years for each patient were met by 38 patients, representing 22 ACL reconstructions, 5 PCL reconstructions, 3 OTHER surgeries, including the Larson technique and medial patellofemoral ligament (MPFL) reconstruction and 8 COMPLEX surgeries. The main endpoints were rerupture and complication rate. Secondary endpoints included stability of the knee (Lachman test, Pivot shift test) and the range of motion.
RESULTS
The rerupture rate was 7.9% (3 grafts). Reruptures only occurred in the ACL group. No reruptures were observed in the PCL, OTHER and COMPLEX surgery groups. Stability improved significantly after surgery and the range of motion returned to values similar to that of healthy knees.
CONCLUSIONS
Tendon allografts sterilized with peracetic acid show promising low rerupture rates and good clinical scores and the results are comparable to the literature on autografts and other allografts.
Topics: Humans; Male; Female; Retrospective Studies; Adult; Tendons; Middle Aged; Sterilization; Allografts; Peracetic Acid; Anterior Cruciate Ligament Reconstruction; Posterior Cruciate Ligament Reconstruction; Posterior Cruciate Ligament; Transplantation, Homologous
PubMed: 38498205
DOI: 10.1007/s00132-024-04486-7 -
Heliyon Mar 2024The exacerbated global water scarcity and stricter water directives are leading to an increment in the recycled water use, requiring the development of new... (Review)
Review
The exacerbated global water scarcity and stricter water directives are leading to an increment in the recycled water use, requiring the development of new cost-effective advanced water treatments to provide safe water to the population. In this sense, peracetic acid (PAA, CHC(O)OOH) is an environmentally friendly disinfectant with the potential to challenge the dominance of chlorine in large wastewater treatment plants in the near future. PAA can be used as an alternative oxidant to HO to carry out the Fenton reaction, and it has recently been proven as more effective than HO towards emerging pollutants degradation at circumneutral pH values and in the presence of anions. PAA activation by homogeneous and heterogeneous iron-based materials generates - besides HO and FeO - more selective CHC(O)O and CHC(O)OO radicals, slightly scavenged by typical HO quenchers (e.g., bicarbonates), which extends PAA use to complex water matrices. This is reflected in an exponential progress of iron-PAA publications during the last few years. Although some reviews of PAA general properties and uses in water treatment were recently published, there is no account on the research and environmental applications of PAA activation by Fe-based materials, in spite of its gratifying progress. In view of these statements, here we provide a holistic review of the types of iron-based PAA activation systems and analyse the diverse iron compounds employed to date (e.g., ferrous and ferric salts, ferrate(VI), spinel ferrites), the use of external ferric reducing/chelating agents (e.g., picolinic acid, l-cysteine, boron) and of UV-visible irradiation systems, analysing the mechanisms involved in each case. Comparison of PAA activation by iron vs. other transition metals (particularly cobalt) is also discussed. This work aims at providing a thorough understanding of the Fe/PAA-based processes, facilitating useful insights into its advantages and limitations, overlooked issues, and prospects, leading to its popularisation and know-how increment.
PubMed: 38495153
DOI: 10.1016/j.heliyon.2024.e27036 -
Chemosphere Apr 2024The presented research concerns the use of nickel cobaltite nanoparticles (NiCoO NPs) for the heterogeneous activation of peracetic acid and application of NiCoO-PAA...
The presented research concerns the use of nickel cobaltite nanoparticles (NiCoO NPs) for the heterogeneous activation of peracetic acid and application of NiCoO-PAA system for degradation 10 organic micropollutants from the group of bisphenols. The bisphenols removal (initial concentration 1 μM) process was optimized by selecting the appropriate process conditions. The optimal amount of catalyst (115 mg/L), peracetic acid (PAA) concentration (7 mM) and pH (7) were determined using response surface analysis in the Design of Experiment. Then, NiCoO NPs were used to check the possibility of reuse in subsequent oxidation cycles. The work also attempts to explain the mechanism of oxidation of the studied micropollutants. The participation of the sorption process on the catalyst was excluded and based on the experiments with radical scavengers it can be concluded that the oxidation proceeds in a radical pathway, mainly with participation of O radicals. Experiments conducted in real water matrices exhibit low impact on degradation efficiency. Toxicity tests with green alga Acutodesmus obliquus and aquatic plant Lemna minor showed that post-reaction mixture influenced growth and the content of photosynthetic pigments in concentration dependent manner.
Topics: Oxidants; Peracetic Acid; Hydrogen Peroxide; Nickel; Water Pollutants, Chemical; Oxidation-Reduction; Benzhydryl Compounds; Minerals; Phenols; Araceae
PubMed: 38494005
DOI: 10.1016/j.chemosphere.2024.141684 -
Frontiers in Microbiology 2024Targeted amplicon sequencing of the 16S rRNA delineates the complex microbial interactions that occur during food spoilage, providing a tool to intensively screen...
INTRODUCTION
Targeted amplicon sequencing of the 16S rRNA delineates the complex microbial interactions that occur during food spoilage, providing a tool to intensively screen microbiota response to antimicrobial processing aids and interventions. The current research determines the microbiota and spoilage indicator (total aerobes and lactic acid bacteria; LAB) response to inorganic and organic antimicrobial intervention use on the shelf-life of fresh, never-frozen, skin-on, bone-in chicken wings.
METHODS
Wings (=200) were sourced from local processor and either not treated (NT) or treated with 15-s dips of tap water (TW), organic (peracetic acid; PAA), inorganic acids (sodium bisulfate; SBS), and their combination (SBS + PAA). Wings were stored (4°C) and rinsed in neutralizing Buffered Peptone Water (BPW) for 1 min on d 0, 7, 14, and 21 post-treatment. Spoilage indicators, aerobic mesophiles and LAB, were quantified from rinsates. Genomic DNA of d 14 and 21 rinsates were extracted, and V4 of 16S rRNA gene was sequenced. Sequences were analyzed using QIIME2.2019.7. APC and LAB counts were reported as Log CFU/g of chicken and analyzed in R Studio as a General Linear Model using ANOVA. Pairwise differences were determined using Tukey's HSD (P£0.05).
RESULTS
Spoilage was indicated for all products by day 21 according to APC counts (>7 Log CFU/g); however, wings treated with SBS and SBS + PAA demonstrated a 7-day extended shelf-life compared to those treated with NT, TW, or PAA. The interaction of treatment and time impacted the microbial diversity and composition ( < 0.05), with those treated with SBS having a lower richness and evenness compared to those treated with the controls (NT and TW; < 0.05, Q < 0.05). On d 14, those treated with SBS and SBS + PAA had lower relative abundance of typical spoilage population while having a greater relative abundance of spp. (~70 and 50% of population; ANCOM < 0.05). By d 21, the spp. populations decreased below 10% of the population among those treated with SBS and SBS + PAA.
DISCUSSION
Therefore, there are differential effects on the microbial community depending on the chemical intervention used with organic and inorganic acids, impacting the microbial ecology differently.
PubMed: 38476936
DOI: 10.3389/fmicb.2024.1348159 -
Infection, Genetics and Evolution :... Apr 2024Listeria monocytogenes is an important human pathogen with a high mortality rate. Consumption of contaminated ready-to-eat food is the main mode of transmission to...
Listeria monocytogenes is an important human pathogen with a high mortality rate. Consumption of contaminated ready-to-eat food is the main mode of transmission to humans. Disinfectant-tolerant L. monocytogenes have emerged, which are believed to have increased persistence potential. Elucidating the mechanisms of L. monocytogenes disinfectant tolerance has been the focus of previous studies using pure cultures. A limitation of such approach is the difficulty to identify strains with reduced susceptibility due to inter-strain variation and the need to screen large numbers of strains and genes. In this study, we applied a novel metagenomic approach to detect genes associated with disinfectant tolerance in mixed L. monocytogenes planktonic communities. Two communities, consisting of 71 and 80 isolates each, were treated with the food industry disinfectants benzalkonium chloride (BC, 1.75 mg/L) or peracetic acid (PAA, 38 mg/L). The communities were subjected to metagenomic sequencing and differences in individual gene abundances between biocide-free control communities and biocide-treated communities were determined. A significant increase in the abundance of Listeria phage-associated genes was observed in both communities after treatment, suggesting that prophage carriage could lead to an increased disinfectant tolerance in mixed L. monocytogenes planktonic communities. In contrast, a significant decrease in the abundance of a high-copy emrC-harbouring plasmid pLmN12-0935 was observed in both communities after treatment. In PAA-treated community, a putative ABC transporter previously found to be necessary for L. monocytogenes resistance to antimicrobial agents and virulence, was among the genes with the highest weight for differentiating treated from control samples. The undertaken metagenomic approach in this study can be applied to identify genes associated with increased tolerance to other antimicrobials in mixed bacterial communities.
Topics: Humans; Listeria monocytogenes; Disinfectants; Benzalkonium Compounds; Listeria; Food Industry; Drug Resistance, Bacterial; Food Microbiology
PubMed: 38467173
DOI: 10.1016/j.meegid.2024.105582 -
Journal of Hazardous Materials May 2024The activation of peracetic acid (PAA) by activated carbon (AC) is a promising approach for reducing micropollutants in groundwater. However, to harness the PAA/AC...
Unlocking the power of activated carbon-mediated peracetic acid activation for efficient antibiotics abatement in groundwater: Coupling the processes of electron transfer, radical production, and adsorption.
The activation of peracetic acid (PAA) by activated carbon (AC) is a promising approach for reducing micropollutants in groundwater. However, to harness the PAA/AC system's potential and achieve sustainable and low-impact groundwater remediation, it is crucial to quantify the individual contributions of active species. In this study, we developed a combined degradation kinetic and adsorption mass transfer model to elucidate the roles of free radicals, electron transfer processes (ETP), and adsorption on the degradation of antibiotics by PAA in groundwater. Our findings reveal that ETP predominantly facilitated the activation of PAA by modified activated carbon (AC600), contributing to ∼61% of the overall degradation of sulfamethoxazole (SMX). The carbonyl group (CO) on the surface of AC600 was identified as a probable site for the ETP. Free radicals contributed to ∼39% of the degradation, while adsorption was negligible. Thermodynamic and activation energy analyses indicate that the degradation of SMX within the PAA/AC600 system requires a relatively low energy input (27.66 kJ/mol), which is within the lower range of various heterogeneous Fenton-like reactions, thus making it easily achievable. These novel insights enhance our understanding of the AC600-mediated PAA activation mechanism and lay the groundwork for developing efficient and sustainable technologies for mitigating groundwater pollution. ENVIRONMENTAL IMPLICATION: The antibiotics in groundwater raises alarming environmental concerns. As groundwater serves as a primary source of drinking water for nearly half the global population, the development of eco-friendly technologies for antibiotic-contaminated groundwater remediation becomes imperative. The innovative PAA/AC600 system demonstrates significant efficacy in degrading micropollutants, particularly sulfonamide antibiotics. By integrating degradation kinetics and adsorption mass transfer models, this study sheds light on the intricate mechanisms involved, emphasizing the potential of carbon materials as sustainable tools in the ongoing battle for clean and safe groundwater.
Topics: Anti-Bacterial Agents; Peracetic Acid; Oxidation-Reduction; Charcoal; Adsorption; Electrons; Water Pollutants, Chemical; Hydrogen Peroxide; Sulfamethoxazole; Groundwater
PubMed: 38430597
DOI: 10.1016/j.jhazmat.2024.133911 -
Clinical Orthopaedics and Related... Jun 2024Peracetic acid and irradiation are common sterilization methods for allograft tendons; however, under some conditions, both methods adversely affect the fiber... (Comparative Study)
Comparative Study
BACKGROUND
Peracetic acid and irradiation are common sterilization methods for allograft tendons; however, under some conditions, both methods adversely affect the fiber arrangement and ultimate load of the tendon. An in vitro study showed that low-dose peracetic acid combined with irradiation may be less detrimental to allograft tendon structure and properties, possibly because the breakdown of peracetic acid can lead to an enlargement of the interstitial spaces and an increase in porosity.
QUESTIONS/PURPOSES
Using a rabbit Achilles tendon model, we asked: What is the effect of peracetic acid-ethanol combined irradiation on (1) the histopathology and fiber diameter of the allograft tendon, (2) tensile creep and load-to-failure biomechanical properties of allograft tendons, and (3) healing of the treated tendon in vivo compared with fresh-frozen allograft and peracetic acid-ethanol sterilization at 4 and 8 weeks?
METHODS
The Achilles tendons used in this study were sourced from euthanized 10-week-old male New Zealand White rabbits previously used for ophthalmic experiments. All allografts were divided into three groups: fresh-frozen group (control group, n = 20), peracetic acid-ethanol sterilization group (n =20), and peracetic acid-ethanol combined irradiation group (n = 20). The sterilization protocols were performed per a predetermined plan. In the peracetic acid-ethanol sterilization group, the tendon tissues were covered with the peracetic acid-ethanol sterilization solution (1% peracetic acid for 30 minutes). In the peracetic acid-ethanol combined irradiation group, the tendon tissues were covered with the peracetic acid-ethanol sterilization solution (0.2% peracetic acid for 30 minutes) and were subjected to 15 kGy gamma irradiation. Thirty 10-week-old male New Zealand White rabbits received bilateral Achilles tendon allografts surgically. Tendon samples from each group were harvested at 4 weeks (n = 30) and 8 weeks (n = 30) postoperatively. For each timepoint, eight tissues were used for histologic staining and electron microscopy, 15 tissues were used for biomechanical testing, and seven tissues were used for hydroxyproline assay and quantitative polymerase chain reaction. Histopathology was determined qualitatively by hematoxylin and eosin and Masson staining, while fiber diameter was measured quantitatively by transmission electron microscopy. Biomechanical properties were measured using cyclic loading tests and load-to-failure tests. The healing outcome was quantitatively judged through healing-related genes and proteins.
RESULTS
At 4 weeks and 8 weeks postoperatively, the peracetic acid-ethanol combined irradiation group visually demonstrated the best continuity and minimal peripheral adhesions. Histologic staining showed that tendon fibers in the peracetic acid-ethanol combined irradiation group maintained consistent alignment without notable disruptions or discontinuities, and there was a qualitatively observed increase in the number of infiltrating cells compared with the control group at the 4-week timepoint (444 ± 49 /mm 2 versus 256 ± 43 /mm 2 , mean difference 188 /mm 2 [95% confidence interval 96 to 281]; p < 0.001). At 8 weeks postoperatively, the tendon fiber diameter in the peracetic acid-ethanol combined irradiation groups was similar to that of the control group (0.23 ± 0.04 µm versus 0.21 ± 0.03 µm, mean difference 0.02 µm [95% CI -0.04 to 0.08]; p = 0.56). At 8 weeks postoperatively, the peracetic acid-ethanol combined irradiation group exhibited better properties in terms of both ultimate load (129 ± 15 N versus 89 ± 20 N, mean difference 40 N [95% CI 7 to 73]; p = 0.02) and energy absorption density (17 ± 6 kJ/m 2 versus 8 ± 4 kJ/m 2 , mean difference 8 kJ/m 2 [95% CI 0.7 to 16]; p = 0.004) compared with the control group. Gene expression analysis revealed higher expression levels of COL1A1 (2.1 ± 0.8 versus 1.0 ± 0, mean difference 1.1 [95% CI 0.1 to 2.1]; p = 0.003) and MMP13 (2.0 ± 0.8 versus 1.0 ± 0, mean difference 1.0 [95% CI 0.4 to 1.6]; p = 0.03) in the peracetic acid-ethanol combined irradiation group than in the control group. There was a higher amount of collagen Type I in tendons treated with peracetic acid-ethanol combined irradiation than in the control group (0.36 ± 0.03 versus 0.31 ± 0.04, mean difference 0.05 [95% CI 0.01 to 0.09]; p = 0.02).
CONCLUSION
Treatment with peracetic acid-ethanol combined irradiation did not have any discernible adverse effect on the histology, fiber diameter, enzymatic resistance, collagen content, or biomechanical strength of the allograft tendons compared with the control group. Peracetic acid-ethanol combined irradiation treatment had a positive impact on remodeling of the extracellular matrix and realignment of collagen fibers.
CLINICAL RELEVANCE
This sterilization method could be helpful to expand the scope and frequency with which allogeneic materials are applied. The long-term healing effect and strength of allograft tendons must be tested before clinical use, and it is necessary to conduct comparative studies on autografts and synthetic materials that are currently widely used clinically.
Topics: Animals; Rabbits; Male; Wound Healing; Peracetic Acid; Allografts; Ethanol; Sterilization; Achilles Tendon; Tensile Strength; Biomechanical Phenomena; Time Factors; Tendon Injuries
PubMed: 38427791
DOI: 10.1097/CORR.0000000000003017 -
Animals : An Open Access Journal From... Feb 2024remains a top bacterial pathogen implicated in several food-borne outbreaks, despite the use of antimicrobials and sanitizers during production and processing. While... (Review)
Review
remains a top bacterial pathogen implicated in several food-borne outbreaks, despite the use of antimicrobials and sanitizers during production and processing. While these chemicals have been effective, has shown the ability to survive and persist in poultry processing environments. This can be credited to its microbial ability to adapt and develop/acquire tolerance and/or resistance to different antimicrobial agents including oxidizers, acids (organic and inorganic), phenols, and surfactants. Moreover, there are several factors in processing environments that can limit the efficacy of these antimicrobials, thus allowing survival and persistence. This mini-review examines the antimicrobial activity of common disinfectants/sanitizers used in poultry processing environments and the ability of to respond with innate or acquired tolerance and survive exposure to persists in such environments. Instead of relying on a single antimicrobial agent, the right combination of different disinfectants needs to be developed to target multiple pathways within .
PubMed: 38396546
DOI: 10.3390/ani14040578 -
Journal of Applied Microbiology Mar 2024This study investigated the antimicrobial efficacy of ultrasound technology (US) in combination with two different disinfectants (Disinfectant A and Disinfectant B),...
AIMS
This study investigated the antimicrobial efficacy of ultrasound technology (US) in combination with two different disinfectants (Disinfectant A and Disinfectant B), containing peracetic acid (PAA) and quaternary ammonium compounds (QACs), respectively, against two sporigenic pathogens, Aspergillus brasiliensis and Bacillus subtilis.
METHODS AND RESULTS
The microbicidal activity of the coupled treatment was compared with the use of the disinfectants alone, and the efficacy of the disinfection strategies was evaluated by the log reduction of the population of the microorganism inoculated onto stainless-steel surface. The combination treatment resulted in a log reduction of 5.40 and 3.88 (Disinfectant A + US) against A. brasiliensis and B. subtilis, at 850 and 500 ppm PAA, compared to 265 and 122 (Disinfectant A only). For Disinfectant B, in combination with US, showed a logarithmic reduction of 5.04 and 4.79 against A. brasiliensis and B. subtilis at 078% v v-1 and 392% v v-1 QACs, respectively, vs. 1.58 and 1.64 (Disinfectant B only). Moreover, no colonies or not statistically significant growth was observed within the US bath containing the disinfectant.
CONCLUSIONS
The antimicrobial efficacy of the two disinfectants was greatly enhanced when used in combination with US, and this also makes it possible to avoid the overuse of chemicals for disinfection.
Topics: Disinfectants; Peracetic Acid; Disinfection; Bacillus subtilis
PubMed: 38373840
DOI: 10.1093/jambio/lxae043 -
Scientific Reports Feb 2024For patients who have difficulty in mechanical cleaning of dental appliances, a denture cleaner that can remove biofilm with dense extracellular polymeric substances is...
For patients who have difficulty in mechanical cleaning of dental appliances, a denture cleaner that can remove biofilm with dense extracellular polymeric substances is needed. The purpose of this study is to evaluate the efficacy of diatom complex with active micro-locomotion for removing biofilms from 3D printed dentures. The diatom complex, which is made by doping MnO nanosheets on diatom biosilica, is mixed with HO to generate fine air bubbles continuously. Denture base resin specimens were 3D printed in a roof shape, and Pseudomonas aeruginosa (10 CFU/mL) was cultured on those for biofilm formation. Cleaning solutions of phosphate-buffered saline (negative control, NC), 3% HO with peracetic acid (positive control, PC), denture cleanser tablet (DCT), 3% HO with 2 mg/mL diatom complex M (Melosira, DM), 3% HO with 2 mg/mL diatom complex A (Aulacoseira, DA), and DCT with 2 mg/mL DM were prepared and applied. To assess the efficacy of biofilm removal quantitatively, absorbance after cleaning was measured. To evaluate the stability of long-term use, surface roughness, ΔE, surface micro-hardness, and flexural strength of the 3D printed dentures were measured before and after cleaning. Cytotoxicity was evaluated using Cell Counting Kit-8. All statistical analyses were conducted using SPSS for Windows with one-way ANOVA, followed by Scheffe's test as a post hoc (p < 0.05). The group treated with 3% HO with DA demonstrated the lowest absorbance value, followed by the groups treated with 3% HO with DM, PC, DCT, DCT + DM, and finally NC. As a result of Scheffe's test to evaluate the significance of difference between the mean values of each group, statistically significant differences were shown in all groups based on the NC group. The DA and DM groups showed the largest mean difference though there was no significant difference between the two groups. Regarding the evaluation of physical and mechanical properties of the denture base resin, no statistically significant differences were observed before and after cleaning. In the cytotoxicity test, the relative cell count was over 70%, reflecting an absence of cytotoxicity. The diatom complex utilizing active micro-locomotion has effective biofilm removal ability and has a minimal effect in physical and mechanical properties of the substrate with no cytotoxicity.
Topics: Humans; Denture Bases; Diatoms; Hydrogen Peroxide; Manganese Compounds; Oxides; Biofilms; Printing, Three-Dimensional; Surface Properties; Materials Testing
PubMed: 38369560
DOI: 10.1038/s41598-024-54408-y