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Infection and Drug Resistance 2020The genus are gram-negative bacilli which are completely aerobic, non-motile, non-fermenting and yellow-pigmented with a characteristic fruity odor. species are widely...
The genus are gram-negative bacilli which are completely aerobic, non-motile, non-fermenting and yellow-pigmented with a characteristic fruity odor. species are widely found in the environment, especially in water and soil, and are considered as low-grade opportunistic pathogens for humans. infections are most commonly seen in immunocompromised patients and only rarely occur in immunocompetent patients. We here report the first confirmed catheter-related bloodstream infection (CRBSI) due to in an immunocompetent patient. We also review the literature related to infections.
PubMed: 32021328
DOI: 10.2147/IDR.S234778 -
Scientific Reports Dec 2019The black blow fly, Phormia regina (Meigen) (Diptera: Calliphoridae) is one of the most abundant carrion flies in North America. Calliphorids are important in...
The black blow fly, Phormia regina (Meigen) (Diptera: Calliphoridae) is one of the most abundant carrion flies in North America. Calliphorids are important in agriculture and animal production, veterinary sciences, forensics and medical entomology. While the role of flies in the epidemiology of human and animal diseases is an active area of research, little is known about the microorganisms associated with these insects. We examined the diversity of wild-caught black blow fly endogenous (internal body) and exogenous (external body) microbial communities using 16S rRNA gene sequencing. Overall, 27 phyla, 171 families and 533 genera were detected, and diversity was significantly higher (P < 0.05) on external body surfaces. At the genus level, Dysgonomonas, Ignatzschineria, Acinetobacter, Vagococcus, Myroides, and Wohlfahrtiimonas were predominant. Cloning and sequencing of nearly full-length fragments of the 16S rRNA gene showed that some of the species identified are known to be pathogenic to humans, animals, and plants. Myroides odoratimimus and Acinetobacter radioresistens are well-known, multi-drug resistant bacteria. These results provide a snapshot of the microbial communities harbored by adult black blow flies and call for more comprehensive studies to better characterize the role these flies may play in the transmission of pathogenic microorganisms.
Topics: Animals; Biodiversity; Diptera; Farms; High-Throughput Nucleotide Sequencing; Humans; Metabolome; Metabolomics; Microbiota; Phylogeny; RNA, Ribosomal, 16S
PubMed: 31889104
DOI: 10.1038/s41598-019-56733-z -
IDCases 2019spp., formerly ., are gram-negative, non-motile, traditionally opportunistic pathogens that are infrequent clinical isolates. spp. have been pathogenically implicated...
spp., formerly ., are gram-negative, non-motile, traditionally opportunistic pathogens that are infrequent clinical isolates. spp. have been pathogenically implicated in only 52 reported cases since the discovery of the genus in the 1920s. We present a case of spp. lower extremity cellulitis and bacteremia. To our knowledge, this is the 16th case of cutaneous infection caused by spp. Etiology of this patient's infection was felt to be related to exposure of his pre-existing lower extremity wounds to soil and water versus a dog lick in the context of relative immunosuppression from type 2 diabetes and chronic inhaled steroid use. Given the characteristic multi-drug resistance of spp., resistance to usual empiric antimicrobials given for cellulitis, and potential for fatal infection in cases of pan-resistance, it is important that clinicians remain alert to the possibility of this rare pathogen.
PubMed: 31538047
DOI: 10.1016/j.idcr.2019.e00638 -
Lung India : Official Organ of Indian... 2019
PubMed: 31464224
DOI: 10.4103/lungindia.lungindia_42_19 -
Open Forum Infectious Diseases Jul 2019spp. are environmental bacterial organisms that rarely cause disease in humans. spp. infections are infrequently reported in the literature, and infections are quite... (Review)
Review
spp. are environmental bacterial organisms that rarely cause disease in humans. spp. infections are infrequently reported in the literature, and infections are quite uncommon. spp. usually infect immunocompromised hosts and can have highly resistant antibiotic susceptibility patterns. Here we report a case of bacteremia and severe cellulitis in a patient with cirrhosis and review the literature of other spp. infections.
PubMed: 31334299
DOI: 10.1093/ofid/ofz282 -
Biocontrol Science 2019The prevalence of antibiotic resistant bacteria in aquaculture has reached alarming proportions and intensified the search for microbe derived antimicrobial...
The prevalence of antibiotic resistant bacteria in aquaculture has reached alarming proportions and intensified the search for microbe derived antimicrobial compounds. This study isolated bacteria from the intestine of Sagor catfish (Hexanematichthys sagor) and screened it for antagonistic properties. Five out of 334 bacterial isolates inhibited growth of fish pathogens. The 5 bacterial strains included relatives of Shewanella haliotis, Myroides odoratimimus, Vibrio harveyi, Vibrio alginolyticus and Alcaligenes faecalis. The growth profiles and probiotic properties of these bacteria were examined. The results showed that the isolate 9 (3) 7.5.2.1, whose closest relative was S. haliotis exhibited growth and probiotic advantage compared to the other bacterial strains, such as highest doubling time and the ability to survive at all experimental temperatures (18 to 60℃) , and bile concentrations (0.01 to 1.00%) and pH (pH2 to 9) . While the bacteria with probiotic properties were successfully isolated. Further study is necessary to examine the efficiency of the probiotic candidate bacteria in boosting fish immunity against pathogens.
Topics: Animals; Antibiosis; Bacteria; Catfishes; Fish Diseases; Intestinal Mucosa; Probiotics
PubMed: 30880308
DOI: 10.4265/bio.24.1 -
Avicenna Journal of Medical... 2019The present study focused on the production of L-asparaginase using Solid State Fermentation (SSF) by
BACKGROUND
The present study focused on the production of L-asparaginase using Solid State Fermentation (SSF) by
METHODS
Initially, five significant parameters (Carbon source; Nitrogen source, temperature, pH and incubation period) were identified that affect the production process of L-asparaginase using Classical One Factor at a Time (OFAT) optimization. An optimized L-asparaginase specific activity obtained by OFAT was recorded as 85.7 . Central Composite Design (CCD) was also employed successively to optimize the multiple parameters at a time and their results were compared.
RESULTS
Maximum L-asparaginase enzyme specific activity obtained by CCD method was 295.6 under the hold values of carbon source (wheat bran) 12 , nitrogen source (yeast extract) 7 , temperature 37°, pH=7.5 and incubation period 47 . Upon validation, the obtained results proved that there was a good relation existing between the experimental and the predicted model (p<0.05). L-asparaginase activity was enhanced in statistical method up to 3.4 folds compared to that of classical method.
CONCLUSION
Utilization of wheat bran as a low cost carbon source in SSF for the production of L-asparaginase enzyme makes the process economical and in turn reduces the environmental pollution by biotransformation to commercially useful bio product.
PubMed: 30800244
DOI: No ID Found -
Antimicrobial Resistance and Infection... 2018spp. are common environmental organisms and they can be isolated predominantly in water, soil, food and in sewage treatment plants. In the last two decades, an...
BACKGROUND
spp. are common environmental organisms and they can be isolated predominantly in water, soil, food and in sewage treatment plants. In the last two decades, an increasing number of infections such as urinary tract infections and skin and soft tissue infections, caused by these microorganisms has been reported. Selection of appropriate antibiotic therapy to treat the infections caused by . is difficult due to the production of a biofilm and the organism's intrinsic resistance to many antibiotic classes.
CASE PRESENTATION
We report the case of a 69-year-old immunocompromised patient who presented with repeated episodes of macroscopic haematuria, from Northern Italy.A midstream urine sample cultured a Gram negative rod in significant amounts (> 10 colony-forming units (cfu)/mL), which was identified as The patient was successfully treated with trimethoprim/sulfamethoxazole after antibiotic susceptibility testing confirmed its activity.
CONCLUSION
This case underlines the emergence of multidrug resistant spp. which are ubiquitous in the environment and it demands that clinicians should be more mindful about the role played by atypical pathogens, which may harbour or express multidrug resistant characteristics, in immunocompromised patients or where there is a failure of empiric antimicrobial therapy.
Topics: Aged; Anti-Bacterial Agents; Drug Resistance, Multiple, Bacterial; Flavobacteriaceae; Flavobacteriaceae Infections; Humans; Immunocompromised Host; Male; Microbial Sensitivity Tests; Sulfamethoxazole; Trimethoprim; Urinary Tract Infections
PubMed: 30094005
DOI: 10.1186/s13756-018-0391-4 -
Infection and Drug Resistance 2018We report an outbreak of urinary tract infections (UTIs) caused by , which occurred in the largest clinical hospital in western Romania.
PURPOSE
We report an outbreak of urinary tract infections (UTIs) caused by , which occurred in the largest clinical hospital in western Romania.
PATIENTS AND METHODS
From June to August 2017, four strains of were isolated from the urine samples of patients hospitalized in the urology, diabetes, and surgery departments. Hospital records of all patients whose urine cultures were positive for were reviewed retrospectively. We also reviewed the cases reported in the literature.
RESULTS
All UTIs, except one, were hospital-acquired infections. All patients with UTIs were immunocompromised. Three patients underwent urinary catheterization with a Foley's catheter upon admission in the emergency department and one presented for replacement of ureterostomy tubes. All isolates were resistant to almost all the tested antibiotics. Two patients were successfully treated with tigecycline and one was receiving antimicrobial treatment for another infection at the time of isolation of the microorganism.
CONCLUSION
Although is an uncommon pathogen, clinicians should be aware of its ability to cause UTI outbreaks, especially in the immunocompromised population. Due to its multi-drug resistance, it is important to rapidly identify spp. in order to choose the best treatment regimen.
PubMed: 29849466
DOI: 10.2147/IDR.S161069 -
MicrobiologyOpen Feb 2019Myroides odoratimimus is an important nosocomial pathogen. Management of M. odoratimimus infection is difficult owing to the multidrug resistance and the unknown... (Comparative Study)
Comparative Study
Myroides odoratimimus is an important nosocomial pathogen. Management of M. odoratimimus infection is difficult owing to the multidrug resistance and the unknown pathogenesis mechanisms. Based on our previous genomic sequencing data of M. odoratimimus PR63039 (isolated from a patient with the urinary tract infection), in this study, we further performed comparative genomic analysis for 10 selected Myroides strains. Our results showed that these Myroides genome contexts were very similar and phylogenetically related. Various prophages were identified in the four clinical isolate genomes, which possibly contributed to the genome evolution among the Myroides strains. CRISPR elements were only detected in the two clinical (PR63039 and CCUG10230) isolates and two environmental (CCUG12700 and H1bi) strains. With more stringent cutoff parameters in CARD analysis, the four clinical M. odoratimimus contained roughly equal antibiotic resistance genes, indicating their similar antibiotic resistance profiles. The three clinical (CCUG10230, CCUG12901, CIP101113) and three environmental (CCUG12700, L41, H1bi) M. odoratimimus strains were speculated to carry the indistinguishable virulent factors (VFs), which may involve in the similar pathogenesis mechanism. Moreover, some VFs might confer to the high capacity of dissemination, attacking tissue cells and induction of autoimmune complications. Our results facilitate the research of antibiotic resistance and the development of therapeutic regimens for the M. odoratimimus infections.
Topics: Drug Resistance, Bacterial; Evolution, Molecular; Flavobacteriaceae; Flavobacteriaceae Infections; Genes, Bacterial; Genome, Bacterial; Genomics; Humans; Phylogeny; Virulence Factors
PubMed: 29797432
DOI: 10.1002/mbo3.634