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Microorganisms Dec 2020Mobile phones (MPs) of healthcare workers (HCWs) may represent an important source of transmission of infectious agents. This longitudinal study documents the...
Mobile phones (MPs) of healthcare workers (HCWs) may represent an important source of transmission of infectious agents. This longitudinal study documents the contamination of these tools. Ten MPs handled by senior pediatricians were sampled once a week during 23 weeks in three pediatric wards of the University Hospital of Saint-Etienne, France. Cultures were performed for bacteria and multiplex PCR assays for a panel of respiratory and enteric viruses. A questionnaire on hygiene habits regarding phoning and care was filled-in by pediatricians before and after the study. From a total of 230 samples, 145 (63%) were contaminated by at least one pathogen. The MPs from emergency departments were the most impacted. Viruses were detected in 179 samples; bacteria were isolated in 59 samples. Contamination increased during the winter epidemic peak. A cross-contamination by between hands and MPs of different HCWs was demonstrated. The communication of the study results influenced the hygiene behaviors. This study highlights the contamination of MPs by pathogens that are resistant in the environment, and its sustainability along the winter season. The role of MPs as vectors of nosocomial infection needs to be better investigated.
PubMed: 33339327
DOI: 10.3390/microorganisms8122011 -
Microorganisms Oct 2020Long considered to be a consequence of human antibiotics use by deduction, antibiotic resistance mechanisms appear to be in fact a much older phenomenon as antibiotic...
Long considered to be a consequence of human antibiotics use by deduction, antibiotic resistance mechanisms appear to be in fact a much older phenomenon as antibiotic resistance genes have previously been detected from millions of year-old permafrost samples. As these specimens guarantee the viability of archaic bacteria, we herein propose to apply the culturomics approach to recover the bacterial content of a Siberian permafrost sample dated, using the in situ-produced cosmogenic nuclide chlorine36 (Cl), at 2.7 million years to study the dynamics of bacterial evolution in an evolutionary perspective. As a result, we cultured and sequenced the genomes of 28 ancient bacterial species including one new species. To perform genome comparison between permafrost strains and modern isolates we selected 7 of these species (i.e., and ). We observed a high level of variability in genomic content with a percentage of shared genes in the core genomes ranging from 21.23% to 55.59%. In addition, the Single Nucleotide Polymorphism (SNP) comparison between permafrost and modern strains for the same species did not allow a dating of ancient strains based on genomic content. There were no significant differences in antibiotic resistance profiles between modern and ancient isolates of each species. Acquired resistance to antibiotics was phenotypically detected in all gram-negative bacterial species recovered from permafrost, with a significant number of genes coding for antibiotic resistance detected. Taken together, these findings confirm previously obtained data that antibiotic resistance predates humanity as most of antimicrobial agents are natural weapons used in inter-microbial conflicts within the biosphere.
PubMed: 33023015
DOI: 10.3390/microorganisms8101522 -
3 Biotech May 2020This work investigated the properties of VKM B-3302 bacteria isolated from activated sludge and immobilized in an N-vinylpyrrolidone-modified poly(vinyl alcohol)...
This work investigated the properties of VKM B-3302 bacteria isolated from activated sludge and immobilized in an N-vinylpyrrolidone-modified poly(vinyl alcohol) matrix. The developed hydrogel formed a network structure to enable the entrapment of microbial cells with their viability and biocatalytic properties preserved, which ensured the technological possibility of replicating expendable biosensor receptor elements. A new ratio of the components for the synthesis selected in this work enabled producing a copolymer of an earlier undescribed chemical structure, which can be efficiently used for immobilization of highly sensitive bacteria. A biological oxygen demand (BOD) biosensor with these bacteria and matrix was shown to possess a long-time stability exceeding that described earlier, to have a broad substrate specificity and to exceed approximately tenfold the nearest analogues by its sensitivity and the lower boundary value of 0.05 mg/dm. The biosensor enabled assays of water samples initially attributed to pure samples (the BOD range, 0.05-5.0 mg/dm). BOD assays of water samples from various sources showed the use of the receptor element of this composition to enable the data that closely correlated with the standard method ( = 0.9990).
PubMed: 32346498
DOI: 10.1007/s13205-020-02199-0 -
IDCases 2019is a Gram-negative coccobacilli which is often an environmental organism. However, infection of patients usually with underlying immunosuppression has been described in...
INTRODUCTION
is a Gram-negative coccobacilli which is often an environmental organism. However, infection of patients usually with underlying immunosuppression has been described in the last decades, mainly due to the emergence of diagnostic molecular methods.
CASE PRESENTATION
We describe here a case of peritonitis in a patient undergoing peritoneal dialysis. Turbidity of the peritoneal dialysate was the sole clinical manifestation. Inflammatory markers were not raised. A peritoneal fluid specimen showed increased white-cell count, but no organisms were seen on Gram stain. identified as the infectious agent. Patient was successfully treated with gentamicin. Minimum inhibitory concentration analysis suggested to be sensitive to aminoglycosides and specific betalactams but not to ciprofloxacin and ceftazidime, in line with previous literature.
DISCUSSION
This case of peritoneal-dialysis peritonitis contributes to accumulating evidence on the emergent role of this organism as a relevant human pathogen. It also provides information about antibiotic resistance patterns that helps to guide therapy more specifically and effectively.
PubMed: 30701158
DOI: 10.1016/j.idcr.2019.e00486 -
Frontiers in Microbiology 2018Bacteria of the genus are common components of the microbiomes of many naturally- and anthropogenically shaped environments. One species, , is unique within the genus...
Bacteria of the genus are common components of the microbiomes of many naturally- and anthropogenically shaped environments. One species, , is unique within the genus because it is associated with opportunistic human infections. Therefore, strains of may serve as an interesting model to study the transition from a saprophytic to a pathogenic lifestyle in environmental bacteria. Unfortunately, knowledge concerning the biology, genetics and genomic content of is fragmentary; also the mechanisms of pathogenicity of this bacterium remain unclear. In this study we provide the first insight into the genome composition and metabolic potential of a clinical isolate, CCUG 32053. This strain has a multipartite genome (4,632,079 bp) composed of a circular chromosome plus eight extrachromosomal replicons pYEE1-8: 3 chromids and 5 plasmids, with a total size of 1,247,173 bp. The genome has been significantly shaped by the acquisition of genomic islands, prophages ( and phage families) and numerous insertion sequences (ISs) representing seven IS families. Detailed comparative analysis with other complete genomic sequences of spp. (including FDAARGOS_252 and TT13, as well as non-pathogenic strains of other species in this genus) enabled us to identify species-specific genes and to predict putative determinants of virulence. This is the first attempt to identify pathoadaptive genetic information of and to estimate the role of the mobilome in the evolution of pathogenicity in this species.
PubMed: 30410477
DOI: 10.3389/fmicb.2018.02553 -
Genome Announcements Jan 2018TT13 was isolated from human skin because of its ability to degrade propylene glycol. Here, we present the whole-genome sequence of this strain; it possesses one...
TT13 was isolated from human skin because of its ability to degrade propylene glycol. Here, we present the whole-genome sequence of this strain; it possesses one 3.58-Mb chromosome and six plasmids. TT13 genome analysis indicated that this bacterium has denitrification potential.
PubMed: 29348361
DOI: 10.1128/genomeA.01514-17 -
Nefrologia : Publicacion Oficial de La... 2016
Topics: Animals; Catheter-Related Infections; Dogs; Environmental Exposure; Female; Gram-Negative Bacterial Infections; Humans; Middle Aged; Paracoccus; Peritoneal Dialysis; Peritonitis; Soil Microbiology
PubMed: 27039709
DOI: 10.1016/j.nefro.2016.02.009