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Food Science & Nutrition Jun 2023The purpose of this study was to investigate the effects of the active ingredients of barley lees on the physiological indexes, intestinal flora, and liver transcriptome...
Effects of liposoluble components of highland barley spent grains on physiological indexes, intestinal microorganisms, and the liver transcriptome in mice fed a high-fat diet.
The purpose of this study was to investigate the effects of the active ingredients of barley lees on the physiological indexes, intestinal flora, and liver transcriptome of mice fed a high-fat diet. Twenty-four male C57BL/6J mice were randomly divided into 4 groups and fed the experimental diets for 5 weeks. The results showed that the fat-soluble components of distillers' grains significantly reduced body weight, abdominal fat, perirenal fat, blood glucose, low-density lipoprotein cholesterol, triglycerides, and total cholesterol in the high-fat diet-fed mice ( < .05), significantly decreased alanine aminotransferase and malondialdehyde levels, and significantly increased total superoxide dismutase, catalase, reduced glutathione and glutathione peroxidase levels ( < .05). At the phylum level, lipid-soluble components significantly increased the abundance of and decreased the ratio. At the genus level, the relative abundances of and were increased. Transcriptomic analysis showed that lipid-soluble components of spent grains reduced the mRNA expression of ANGPTL8, CD36, PLTP, and SOAT1 and increased the mRNA expression of CYP7A1 and ABCA1 in the cholesterol metabolism pathway, promoted the transport of cholesterol, and inhibited the absorption of cholesterol, which can decrease cholesterol levels by speeding up the conversion of cholesterol into bile acids.
PubMed: 37324893
DOI: 10.1002/fsn3.3291 -
Journal of Kidney Cancer and VHL 2023Renal angiomyolipoma is an uncommon, benign-mixed mesenchymal tumor consisting of thick-walled blood vessels, smooth muscles, and mature adipose tissues. Twenty percent...
Renal angiomyolipoma is an uncommon, benign-mixed mesenchymal tumor consisting of thick-walled blood vessels, smooth muscles, and mature adipose tissues. Twenty percent of these tumors are associated with tuberous sclerosis. Wunderlich syndrome (WS), an acute nontraumatic spontaneous perirenal hemorrhage, can be a presentation of large angiomyolipoma. This study evaluated the presentation, management, and complications of renal angiomyolipoma with WS in eight patients who presented to the emergency department between January 2019 and December 2021. The presenting symptoms included flank pain, palpable mass, hematuria, and bleeding in the perinephric space on computerized tomography. Demographic data, symptoms at presentation, comorbidities, hemodynamic parameters, the association with tuberous sclerosis, transfusion requirements, need for angioembolization, surgical management, Clavien-Dindo complication, duration of hospital stay, and 30-day readmission rates were evaluated. The mean age of presentation was 38 years. Of the eight patients, five (62.5%) were females and 3(37.5%) were males. Two (25%) patients had tuberous sclerosis with angiomyolipoma, and three (37.5%) patients presented with hypotension. The mean packed cell transfusion was three units, and the mean tumor size was 7.85 cm (3.5-25 cm). Three of them (37.5%) required emergency angioembolization to prevent exsanguination. Embolization was unsuccessful in one patient (33%) who underwent emergency open partial nephrectomy, and one (33%) patient developed post-embolization syndrome. A total of six patients underwent elective surgery-four underwent partial nephrectomy (laparoscopic - 1, robotic - 1, open - 2) and two underwent open nephrectomy. Three patients encountered Clavien-Dindo complications (Grade 1, n = 2 and IIIA, n = 2). WS is a rare, life-threatening complication in patients with large angiomyolipoma. Judicious optimization, angioembolization, and prompt surgical intervention will help deliver better outcomes.
PubMed: 37303946
DOI: 10.15586/jkcvhl.v10i2.265 -
Case Reports in Hematology 2023Erdheim-Chester disease (ECD), a rare form of non-Langerhans histiocytosis, is a multisystem disorder. The case reported here refers to a 49-year-old man presenting at...
Erdheim-Chester disease (ECD), a rare form of non-Langerhans histiocytosis, is a multisystem disorder. The case reported here refers to a 49-year-old man presenting at the emergency room with respiratory symptoms. While undergoing diagnostic tests for COVID-19 infection, tomography revealed asymptomatic bilateral perirenal tumors, while renal function remained unaltered. ECD was suggested as an incidental diagnosis and confirmed by core needle biopsy. This report provides a brief description of the clinical, laboratory, and imaging findings in this case of ECD. This diagnosis, albeit rare, should be taken into consideration in the context of incidental findings of abdominal tumors to ensure that treatment, when required, is instituted early.
PubMed: 37303482
DOI: 10.1155/2023/4683188 -
World Journal of Surgical Oncology Jun 2023Targeted therapy combined with immunotherapy is the current first-line treatment for metastatic renal cell carcinoma (mRCC), but patients with tumor thrombus (TT) may...
Long-term outcomes after cytoreductive nephrectomy and thrombectomy of patients with metastatic renal cell carcinoma with venous tumor thrombus: a retrospective study from a large Chinese center.
BACKGROUND
Targeted therapy combined with immunotherapy is the current first-line treatment for metastatic renal cell carcinoma (mRCC), but patients with tumor thrombus (TT) may suffer from lower limb edema or even sudden cardiac death, so the purpose of this study is to investigate the efficacy and safety of surgical treatment in patients with mRCC and TT and explore worse factors to affect the prognosis in this series of patients.
PATIENTS AND METHODS
A total of 85 mRCC patients with TT who received cytoreductive nephrectomy and thrombectomy at our medical center from 2014 to 2023 are included. All patients received postoperative systemic therapy. Overall survival (OS) is defined as the time from surgery to death due to any reason or the last follow-up. Kaplan-Meier analysis was performed to evaluate OS and differences among groups were tested by log-rank. Multivariable Cox proportional hazards analysis was performed to ascertain independent relationships between clinicopathological factors and OS.
RESULTS
The median age of patients was 58 years old. Eleven patients (12.9%) had no symptoms, 39 patients (45.9%) had local symptoms, 15 patients (17.6%) had systemic symptoms, and 20 patients (23.5%) had both. Mayo grade of TT was 0, 1, 2, 3, and 4 for 12, 27, 31, 7, and 8 patients respectively. Fifty-five patients had lung metastasis, 23 had bone metastasis, 16 had liver metastasis, 13 had adrenal metastasis, and 9 had lymph node metastasis. Of all patients, 17 patients had multiple metastases. The median operation time is 289 min and the median intraoperative hemorrhage is 800 ml. Twenty-eight patients experienced postoperative complications, 8 of which were serious complications of modified Clavien grade III or higher. The median OS of all patients was 33 months and median follow up time was 26 months. In multivariate analysis, systemic symptom (p = 0.00753), pathological type (p = 0.0166), sarcomatous degeneration (p = 0.0334), and perirenal fat infiltration (p = 0.0202) are independent predictors of OS.
CONCLUSION
Cytoreductive nephrectomy and thrombectomy is relatively safe and effective for patients with mRCC accompanied by TT. In this series of patients, the worse prognosis is associated with systemic symptoms, non-clear cell carcinoma, sarcomatous degeneration and perirenal fat infiltration.
Topics: Humans; Middle Aged; Carcinoma, Renal Cell; Retrospective Studies; Kidney Neoplasms; Cytoreduction Surgical Procedures; East Asian People; Thrombosis; Nephrectomy; Prognosis; Thrombectomy
PubMed: 37280590
DOI: 10.1186/s12957-023-03048-z -
Frontiers in Pediatrics 2023Indocyanine Green (ICG) fluorescence imaging has been widely used in the surgical treatment of adult renal cancers, but its application in pediatric renal cancers has...
BACKGROUND
Indocyanine Green (ICG) fluorescence imaging has been widely used in the surgical treatment of adult renal cancers, but its application in pediatric renal cancers has rarely been reported. This study aims to summarize the experience of ICG fluorescence imaging in pediatric renal cancers and explores its safety and feasibility.
METHODS
The clinical features, surgical information, ICG administration regimen, near infrared radiography data and ex vivo and pathological results of children with renal cancers using ICG navigation were analyzed and summarized.
RESULTS
There were 7 cases of renal cancer, including 4 cases of Wilms tumor (WT), 1 case of malignant rhabdoid tumor of the kidney (MRTK) and 2 cases of renal cell carcinoma (RCC). By intraoperative intravenous injection of ICG from 2.5 to 5 mg (0.05-0.67 mg/kg), the tumors were visualized in 6 cases or ex vivo, and the tumor visualization failed in 1 case due to renal artery embolization before operation. By injecting 5 mg ICG into the normal renal tissue during the operation, 3 patients achieved fluorescent localization of sentinel lymph nodes. No ICG-related adverse reactions were found in any of the patients during or after operation.
CONCLUSIONS
ICG fluorescence imaging is safe and feasible for renal cancers in children. Intraoperative administration can achieve tumor and sentinel lymph node visualization which will facilitate the development of nephron sparing surgery (NSS). However, the technique is affected by ICG dose, anatomical conditions around the tumor, and renal blood flow. A proper dose of ICG and the complete removal of perirenal fat are helpful for the fluorescence imaging of the tumor. It has potential in the operation of renal cancer in children.
PubMed: 37215593
DOI: 10.3389/fped.2023.1108997 -
Bulletin of Emergency and Trauma 2023Wunderlich syndrome, also known as the spontaneous non-traumatic retroperitoneal hemorrhage, is an uncommon condition characterized by acute, spontaneous, non-traumatic...
Wunderlich syndrome, also known as the spontaneous non-traumatic retroperitoneal hemorrhage, is an uncommon condition characterized by acute, spontaneous, non-traumatic renal hemorrhage into the subcapsular or perirenal spaces. The majority of the cases are caused by renal cell carcinoma or renal angiomyolipoma. Other causes are arteriovenous malformation, cystic renal disease, and anticoagulation medications. The classic presentation is "Lenk's triad" of acute flank pain, palpable flank mass, and hypovolemia. The diagnosis is based on clinical suspicion and confirmed by a CT scan, which is the preferred imaging modality. Due to the rarity of these cases and the wide range of clinical manifestations, the treatment is divergent ranging from conservative management to nephrectomy. Herein, we present a case of massive right renal hemorrhage caused by warfarin toxicity that was initially misdiagnosed as acute renal colic due to the patient's refusal to refer to the clinic during Corona Virus Disease- 19 era and was later managed with a right nephrectomy.
PubMed: 37193006
DOI: 10.30476/BEAT.2023.97913.1415 -
Revista Espanola de Enfermedades... May 2024Cullen´s sign and Grey Turner sign are, respectively, the cutaneous ecchymoses located in periumbilical region and tissues flanks along the lower portion of the...
Cullen´s sign and Grey Turner sign are, respectively, the cutaneous ecchymoses located in periumbilical region and tissues flanks along the lower portion of the abdomen. Both have been observed in <1% of individuals with acute pancreatitis, suggesting poor prognosis in terms of gravity and mortality. However, these signs are not exclusive for acute pancreatitis, as they can appear in other identities such as: ruptured ectopic pregnancy, aortic aneurysm, rectus abdominis muscle hematoma, perforated duodenal ulcer, common bile duct rupture and biliary peritonitis, idiopathic perirenal hemorrhage, infectious mononucleosis with splenic rupture, metastatic esophageal and thyroid cancer, non-Hodgkin lymphoma, amoebic liver abscess, portal hypertension, and liver tumor disease. Based on the review of the literature, it is patent that Cullen´s sign and Grey Turner sign are neither sensitive nor specific for acute pancreatitis, therefore it may be best to relate these findings in the physical examination to conditions associated with abdominal pathology and retroperitoneal hemorrhage. We report the case of a 60-year-old Spanish female, with previous history of squamous small cell neuroendocrine lung carcinoma with hepatic extension in follow-up by palliative care services. Due to disease progression after two palliative chemotherapy sessions, the patient came to the emergency room with jaundice and abdominal pain in superior regions. On physical examination, notable findings included ecchymoses in the right flank and periumbilical region. Laboratory findings showed elevated bilirubin and transaminase levels, as well as a small increase in the serum amylase and lipase levels. Cholangioresonance was performed to discard acute biliary pancreatitis. The imaging revealed no enlargement of the pancreas, dilatation of the common bile duct without visible stone, and a liver full of new cancerous liver implants. These findings were consistent with diffuse distribution metastases lesions, concluding the progression of liver disease.
Topics: Humans; Female; Middle Aged; Ecchymosis; Pancreatitis; Lung Neoplasms; Liver Neoplasms
PubMed: 37170592
DOI: 10.17235/reed.2023.9655/2023 -
Animal : An International Journal of... Jun 2023A total of 197 nulliparous rabbits (from three paternal lines) were used to test potential strategies to overcome the consequences on reproduction associated with the...
A total of 197 nulliparous rabbits (from three paternal lines) were used to test potential strategies to overcome the consequences on reproduction associated with the selection for high growth rate. The R line was selected for growth rate during the growing period for 37 generations, the RF line was founded through a high selection intensity of elite animals of the R line, and the RFLP line, which was obtained by backcrossing RF animals with the LP line (a long-lived productive maternal line, characterised by high resilience). BW, perirenal fat thickness, fertility, daily feed intake, milk yield and blood metabolites of females were controlled from 1st artificial insemination to 3rd parturition. Litter size, litter weight, individual weight and feed ingestion of kits were controlled from birth to weaning. Our results show that RF females were significantly lighter than R and RFLP females throughout the trial (-5.0%; P < 0.05). Furthermore, RF animals had a higher fertility rate than RFLP females, at first cycle (+10.5 percentage points; P < 0.05). However, RFLP had a higher fertility rate than RF females at second cycle (+21.5 percentage points; P < 0.01). On average, RFLP females had higher perirenal fat thickness than R females at parturition (+3.0%; P < 0.05) and higher daily feed intake than of R and RF females during gestation and late lactation (+9.7 and +8.7%, respectively; P < 0.05). RFLP females produced more milk than R and RF females in the two first lactations (+18.5%; P < 0.001). In the first three parturitions, R females delivered fewer kits born alive (-1.7 kits than RF and RFLP; P < 0.05). In addition, R females' blood had a higher concentration of glutamine and glutamate than RFLP (+24 and +22.7%, respectively; P < 0.05). RFLP litters were heavier than both R and RF litters throughout lactation. However, R kits were heavier at birth than RF and RFLP (+7.9%). Results suggest that the foundation of a paternal line using elite animals could generate females with better early reproductive performance. In addition, backcrossing the RF line with a maternal LP line resulted in a genetic line whose females had a different resource allocation strategy to foster reproduction during the studied period.
Topics: Pregnancy; Rabbits; Female; Animals; Reproduction; Lactation; Eating; Weaning; Parturition; Litter Size
PubMed: 37167819
DOI: 10.1016/j.animal.2023.100729 -
Journal of Medical Case Reports May 2023Spontaneous perirenal hemorrhage (Wunderlich syndrome) in the fetus is a rare urinary system disease. Prenatal ultrasound diagnosis presents challenges due to the lack...
BACKGROUND
Spontaneous perirenal hemorrhage (Wunderlich syndrome) in the fetus is a rare urinary system disease. Prenatal ultrasound diagnosis presents challenges due to the lack of specific clinical features.
CASE PRESENTATION
A 27-year-old Chinese woman gravida 2 para 0 found her fetus with the left Wunderlich syndrome accompanying bilateral hydronephroses and bladder dysfunction with an early diagnosis through prenatal ultrasound and postnatal magnetic resonance imaging. After a timely emergency cesarean section, the infant was administrated antimicrobial prophylaxis and an indwelling catheter treatment. Ultrasound follow-up showed his urinary system gradually developed normally.
CONCLUSION
A fetus with bilateral hydronephroses accompanying bladder dysfunction should be observed because of the risk of spontaneous renal rupture with hemorrhage formation. Ultrasound and magnetic resonance imaging play a vital role in the diagnosis and follow-up of Wunderlich syndrome. Early diagnosis facilitates better pregnancy planning and appropriate care of newborns.
Topics: Humans; Infant, Newborn; Pregnancy; Female; Adult; Cesarean Section; Kidney Diseases; Hemorrhage; Abnormalities, Multiple; Fetus; Hydronephrosis
PubMed: 37147736
DOI: 10.1186/s13256-023-03867-4 -
Zhongguo Ying Yong Sheng Li Xue Za Zhi... Sep 2022The effects of Alternate-day modified fasting combined exercise on fat mass, muscle mass, and serum Irisin, FNDC5 and UCP1 proteins were investigated in rats with 4...
OBJECTIVE
The effects of Alternate-day modified fasting combined exercise on fat mass, muscle mass, and serum Irisin, FNDC5 and UCP1 proteins were investigated in rats with 4 weeks of aerobic exercise and modified alternate-day fasting intervention.
METHODS
Thirty-two healthy 8-week-old SPF male SD rats were randomly divided into control group, exercise group, alternate-day modified fasting and alternate-day modified fasting combined with exercise group, 8 rats in each group. The exercise group performed treadmill exercise with moderate exercise intensity(60 min/d,5 d/w), the alternate-day modified fasting group alternated between fasting and free feeding every other day, and fed 25% basal energy feed on fasting days, and the alternate-day modified fasting combined exercise group received two combined interventions. After 4 weeks of intervention, the body fat rate of rats was measured by apical blood sampling and abdominal aortic blood sampling, and the serum was preserved and centrifuged, and the wet weights of bilateral gastrocnemius, bilateral perirenal fat and brown fat at the scapula were weighed, and samples were collected for paraffin sectioning and HE staining, and the cell areas were counted; serum Irisin levels were measured by ELISA, and FNDC5 protein expression in gastrocnemius and UCP1 protein expression in adipose tissue were detected by Western blot.
RESULTS
After 4 weeks of intervention, compared with the Con group, energy intake, body weight and body fat were decreased significantly in the Exer, ADMF and ADMF-Exer groups (<0.05), the wet weight/body weight and adipocyte area of white fat were reduced significantly (<0.01), and there was no significant difference in scapular fat wet weight/body weight (>0.05). Compared with the Con group, the gastrocnemius wet weight/body weight in the ADMF group was reduced significantly (<0.05), while that in the ADMF-Exer group was increased significantly (<0.05), the muscle cross-sectional areas in the Exer group and the ADMF-Exer group were increased (<0.05), and the content of gastrocnemius FNDC5 protein, serum Irisin level and expression of adipose UCP1 protein in the ADMF-Exer group were increased significantly (<0.05). After 4 weeks of intervention, energy intake was reduced significantly in both ADMF and ADMF-Exer groups (<0.01) and body weight of ADMF-Exer group was decreased (<0.05) compared with the Exer group. Compared with the Exer group, there were no significant differences in body fat content, white fat wet weight/body weight and scapular fat wet weight/body weight between ADMF group and ADMF-Exer group (>0.05), and adipocyte area in ADMF-Exer group was reduced significantly (<0.05). Compared with the Exer group, the gastrocnemius muscle wet weight/body weight was reduced significantly in the ADMF group (<0.05), and the expression of FNDC5 protein, serum Irisin and adipose UCP1 protein in the ADMF-Exer group were increased significantly compared with the Exer group (<0.05).
CONCLUSION
Alternate-day modified fasting combined with exercise intervention can effectively control body weight and reduce body fat in rats, and the mechanism may be through the FNDC5/Irisin-UCP1 pathway to induce browning of white adipose tissue and increase thermogenesis of brown fat.
Topics: Male; Rats; Animals; Fibronectins; Uncoupling Protein 1; Rats, Sprague-Dawley; Obesity; Transcription Factors; Adipose Tissue, Brown; Fasting; Blood Proteins; Muscle, Skeletal
PubMed: 37088773
DOI: 10.12047/j.cjap.6323.2022.107