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Cureus Feb 2024Background Teeth serve many functions, and aesthetics is one of the most important aspects served by teeth, perceived by the limbic system of the human brain. The golden...
Background Teeth serve many functions, and aesthetics is one of the most important aspects served by teeth, perceived by the limbic system of the human brain. The golden divine ratio is the unique proportion often correlated with beauty. The present study was devised to estimate the dimension of human permanent canines and approximation to the golden divine ratio. Materials and methods The present study included 47 extracted human permanent canines retrieved from the tooth repository of our institute's Department of Oral Biology. Using digital vernier calipers (Themisto TH-M61 digital vernier caliper, 0-150mm/ 6 inch, JIPVI Ecommerce Pvt. Ltd, India, 2022), the following measurements were taken: Mesio-distal and labiolingual dimensions of the crown, crown length, root length, root to crown ratio (R/C) and the tooth to root ratio (T/R). The data were analyzed using Statistical Package for Social Sciences (SPSS) software version 26. Results All the dimensions' mean and standard deviations were calculated for both maxillary and mandibular canines. While the means of mesio-distal and labiolingual dimensions of the crown approximated the values reported in the literature, there was some variation in crown and root lengths. The mean crown lengths of the upper and lower canines were 10.34mm and 9.76mm, respectively, while the root lengths were 16.52 and 15.54mm, respectively. The R/C of both sets and the T/R of the upper canine only followed the golden ratio. T/R of the lower canine was slightly higher (1.64) Conclusion Although the number of included teeth was less, owing to the fact that canines are rarely extracted, our results provided new values of canines for updation in a unique population. More studies are required for comparative anthropological data updates.
PubMed: 38533140
DOI: 10.7759/cureus.54854 -
PloS One 2024Freshwater fauna is facing an uphill task for survival in the Ganga Basin, India, due to a range of factors causing habitat degradation and fragmentation, necessitating...
Freshwater fauna is facing an uphill task for survival in the Ganga Basin, India, due to a range of factors causing habitat degradation and fragmentation, necessitating conservation interventions. As part of the ongoing efforts to conserve the freshwater fauna of the Basin, we are working on rehabilitating rescued freshwater chelonians. We carry out various interventions to restore rescued individuals to an apparent state of fitness for their release in suitable natural habitats. Morphometric measurements are crucial to managing captive wild animals for assessing their growth and well-being. Measurements are made using manual methods like vernier caliper that are prone to observer error experience and require handling the specimens for extended periods. Digital imaging technology is rapidly progressing at a fast pace and with the advancement of technology. We acquired images of turtles using smartphones along with manual morphometric measurements using vernier calipers of the straight carapace length and straight carapace width. The images were subsequently processed using ImageJ, a freeware and compared with manual morphometric measurements. A significant decrease in the time spent in carrying out morphometric measurements was observed in our study. The difference in error in measurements was, however, not significant. A probable cause for this may have been the extensive experience of the personnel carrying out the measurements using vernier caliper. Digital image processing technology can cause a significant reduction in the stress of the animals exposed to handling during measurements, thereby improving their welfare. Additionally, this can be used in the field to carry out morphometric measurements of free-ranging individuals, where it is often difficult to capture individuals, and challenges are faced in obtaining permission to capture specimens.
Topics: Animals; Animals, Wild; Fresh Water; Image Processing, Computer-Assisted; Turtles
PubMed: 38484004
DOI: 10.1371/journal.pone.0300253 -
Cureus Feb 2024Ankle sprains are prevalent injuries leading to functional impairment. The lateral ankle ligament complex (LLC), comprising the anterior talofibular ligament (ATFL),...
BACKGROUND
Ankle sprains are prevalent injuries leading to functional impairment. The lateral ankle ligament complex (LLC), comprising the anterior talofibular ligament (ATFL), posterior talofibular ligament (PTFL), and calcaneofibular ligament (CFL), is weak and prone to injury. The morphometric data of these ligaments are essential for orthopedic practices, including techniques like direct repair or ATFL reconstruction with autograft/allograft, which are limited in the literature. The present study aims to document the anatomy and morphometry of the LLC.
METHODS
Fifteen adult Indian-origin embalmed cadavers were selected for the study. Ankles with antemortem or postmortem injuries or previous surgical interventions were excluded from the study. After precise dissection of the ankle's anterior and lateral aspects as per Cunningham's dissection manual, ligaments were exposed. Length and width were measured using a digital vernier caliper. Morphological attributes such as shape, orientation, and inter-fiber angles were documented.
RESULTS
The most common shape in ATFL was a single band (53.33%). Inner ATFL fibers merged with the ankle joint capsule in 73.33%. ATFL mean length and width were 14 ± 2.4 mm and 7.6 ± 2.0 mm. The angle between the fibula's long axis and ATFL fibers was 107 ± 22°, and the angle between tibiotalar joint lines and parallel ATFL fibers was 30 ± 9.5°. A single band of CFL was predominant (73.33%). The mean length and width of CFL were 18.4 ± 3.9 mm and 5.2 ± 1.3 mm; the angle between the anterior fibula border's long axes and parallel CFL line was 131°. PTFL length was 20.9 ± 3.3 mm and width was 6.2 ± 1.4 mm. The mean length and width of the anterior inferior talofibular ligament (AiTFL) were 11.7 ± 2.6 mm and 9.5 ± 1.6 mm, and of the posterior inferior talofibular ligament (PiTFL) were 12.8 ± 2.1 mm and 10.4 ± 2 mm.
CONCLUSION
Comprehensive knowledge of these ligaments' anatomy and relationships is vital for clinical examination and ultrasonography. Understanding LLC details aids radiologists and orthopedic surgeons in graft selection, sizing, and precise anatomical structure placement during surgical reconstruction.
PubMed: 38465086
DOI: 10.7759/cureus.53826 -
Journal of Conservative Dentistry and... Feb 2024Esthetic appearance of the face is a major concern in self-image among the majority of the population. The dimensions, anatomy, and arrangement of maxillary anterior are...
CONTEXT
Esthetic appearance of the face is a major concern in self-image among the majority of the population. The dimensions, anatomy, and arrangement of maxillary anterior are the key factors in the esthetic appearance of the face.
AIM
The present study aimed to measure the mesiodistal dimensions of maxillary anterior teeth and analyzing the golden proportion in the representative North Indian population.
MATERIALS AND METHODS
Subjects were seated in the upright position in a dental clinical chair. A digital Vernier caliper was used to record the mesiodistal dimensions of central incisors (CIs), lateral incisor (LI), and canine (C) teeth in maximum smile position. A total of three sets of recordings were made by a single observer to avoid the measurement bias of both left and right sides of teeth. Digital images were also captured. Obtained measurements were used for the calculation of ratios and statistical analysis was done.
RESULTS
The prevalence of golden ratio between right CI/LI was observed in 3.1% males and 3.2% females. The calculated median ratio was 1.2 for males and 1.3 for females which differs significantly ( > 0.05) from the golden ratio. The divine proportion between visible portion of right C/LI was seen in 39.5% males and 32.3% females with calculated median value for males being 0.6 and that for females was 0.7.
CONCLUSION
The prevalence of golden proportion between the CI/LI in esthetically pleasing smiles was too small (mean-3.9 in males and 2.75 in females) while the golden ratio of 0.6 was found in the majority of the population irrespective of the gender.
PubMed: 38463479
DOI: 10.4103/JCDE.JCDE_235_23 -
Cureus Jan 2024Introduction Golden ratio and beauty are two inseparable sides of the same coin and have been studied for centuries by the Greeks. This divine ratio is defined as an...
Introduction Golden ratio and beauty are two inseparable sides of the same coin and have been studied for centuries by the Greeks. This divine ratio is defined as an invincible parameter in aesthetic dentistry to measure looks, symmetry, and balance. Being beautiful and handsome also boosts confidence in today's children and therefore is a top priority for young growing kids. However, there is no study done to define facial measurements based on the golden ratio in preschool and school-going children that can succor aesthetics in formative years. The purpose of this research was to evaluate facial proportions in the vertical dimension, quantify them in reference to the golden ratio, and analyze the association with gender among preschool and school-going children of the Santhal tribe in the Bankura district. Materials and methods A total of 399 subjects, 198 children of 3-5 years with primary teeth and 201 children of 6-12 years with mixed dentition, were selected from schools in villages of Bankura district, West Bengal, India. The subjects were made to relax in a sitting position and a digital vernier caliper was used to record the following vertical facial parameters: total facial height (TFH), trichion-gnathion distance (Tr-Gn), and subnasale-gnathion distance (Sn-Gn). The total facial height was correlated with sexual dimorphism and dentition. The ratio of Tr-Sn/Sn-Gn was calculated and compared with the golden ratio. The ratio was classified as normal (if it was between 1.6 and 1.699, i.e., normal to the golden ratio value), long (if it was more than 1.699, i.e., more than the golden ratio value), or short (if it was below 1.6, i.e., less than the golden ratio value). This facial analysis based on the golden ratio was correlated to sexual dimorphism and dentition. The data were recorded, compared with gender, and statistically analyzed using the unpaired t-test and Chi-square test. Results The total facial height was larger in males than females in both primary and mixed dentition; however, the value was highly significant in primary dentition. Tr-Sn/Sn-Gn ratios were lower in the long facial category in both males and females in both preschool and school-going children. The values were statistically significant in these ratios for both dentitions. Conclusion The majority of children in the Santhal tribe of Bankura in West Bengal did not conform to the golden ratio and showed long faces. There was a significant association of facial features with sexual dimorphism. Clinical significance The early prediction of facial features in children and its confirmation with the established golden ratio can be considered an imperative parameter to comprehend facial aesthetics and symmetry.
PubMed: 38425581
DOI: 10.7759/cureus.53200 -
In Vivo (Athens, Greece) 2024Postoperative scar formation is inevitable, and a gold standard management has not been established to date. Due to the fact long and large scar formation occurs in...
BACKGROUND
Postoperative scar formation is inevitable, and a gold standard management has not been established to date. Due to the fact long and large scar formation occurs in reconstructive surgery, this study analyzed the relationship between various factors in patients who received breast reconstruction using latissimus dorsi (LD) flap to investigate appropriate and effective management approaches.
PATIENTS AND METHODS
Twenty-seven patients who underwent breast reconstruction between June 2014 and January 2015 received laser therapy on their LD donor site at the Kyungpook National University Chilgok Hospital. Scar evaluation was performed on both the surgical scar and intact skin on the contralateral side. Scar evaluation was conducted at five specific points, 2 cm from the midpoint of the scar on each side. Laser treatment was performed at 4-week intervals, and patients were then followed-up for 6 months. To assess scars, gross images were taken using the same settings. In addition, spectrophotometry was used for color assessment, durometer for texture and pressure evaluation, and Vernier calipers and height gauges for a more precise and objective approach.
RESULTS
The mean age of the participants was 45.7 years, and the mean body mass index was 22.1 kg/m The operator-evaluated scar scale scores were 107.2 and 97.3 in the experimental and control groups, respectively. In the patient-rated questionnaire, the scores were 62.3 and 59.4 in the experimental and control groups, respectively.
CONCLUSION
When analyzing early-stage postoperative scars based on various factors, laser therapy is considered a very useful scar management approach. Additionally, when performing reconstructive surgery, tension force is regarded as a significant factor to take into account since it affects scar widening.
Topics: Humans; Middle Aged; Cicatrix; Superficial Back Muscles; Surgical Flaps; Mammaplasty; Laser Therapy; Treatment Outcome
PubMed: 38418124
DOI: 10.21873/invivo.13509 -
Journal of Personalized Medicine Jan 2024Minimally invasive periodontic (perio) surgical procedures, piezocision, and micro-osteoperforation are useful techniques for accelerating tooth movement. These...
BACKGROUND
Minimally invasive periodontic (perio) surgical procedures, piezocision, and micro-osteoperforation are useful techniques for accelerating tooth movement. These techniques also offer advantages in the orthodontic (ortho) and aesthetic domains. This study aimed to evaluate and compare the rates of lower anterior decrowding with piezocision and micro-osteoperforation.
METHODS
This clinical study included 24 patients requiring fixed orthodontic treatments. Two periodontic techniques (piezocision (PZ) and micro-osteoperforation (MOP)) were considered for the orthodontic treatments. Each patient was randomly allocated to either the piezocision (PZ) group or the micro-osteoperforation (MOP) group. The piezocision group received five radiographically guided incisions on the labial surface of the alveolar bone, whereas the micro-osteoperforation group received one to three MOPs each using a mini-implant drill between the six lower anterior teeth, and later, an initial arch wire was ligated to each bracket. Little's irregularity index (LII) was calculated using a digital vernier caliper on study models every four weeks until decrowding was achieved. The difference in the rates of lower anterior crowding between the piezocision and micro-osteoperforation groups was analyzed to determine the statistical significance.
RESULTS
The rates of irregularity index change during decrowding were 4.38 ± 0.61 in the piezocision group and 3.82 ± 0.47 in the micro-osteoperforation group. Piezocision was found to be 1.2 times faster than micro-osteoperforation in terms of the rate of decrowding.
CONCLUSION
The advanced perio-ortho combination technique was advantageous in accelerated decrowding. In comparison to MOP, there was an increase in the rate of decrowding with PZ. Decrowding can be completed quickly with PZ, and it can thus be used to treat crowding effectively in a limited time frame.
PubMed: 38392606
DOI: 10.3390/jpm14020173 -
Human Reproduction (Oxford, England) Apr 2024Do different boys with different types of cryptorchidism exhibit different anogenital distances (AGDs)?
STUDY QUESTION
Do different boys with different types of cryptorchidism exhibit different anogenital distances (AGDs)?
SUMMARY ANSWER
Length of AGD seemed to differ in different groups of patients with cryptorchidism.
WHAT IS KNOWN ALREADY
AGD, which is used as an indicator of prenatal androgen action, tends to be shorter in boys with cryptorchidism compared to unaffected boys. Shorter AGDs have also been reported in boys with hypospadias, in men with poor semen quality, and in men with testicular cancer.
STUDY DESIGN, SIZE, DURATION
A prospective descriptive cohort study was performed using data from consecutively selected boys with cryptorchidism (n = 169) operated in a single center over a period of 3 years (September 2019 to October 2022).
PARTICIPANTS/MATERIALS, SETTING, METHODS
AGD was measured in 169 infant boys, at 3 to 26 months of age, during anesthesia with a vernier caliper measuring the distance from the anus to the base of the scrotum (AGDAS) and from the anus to the anterior base of the penis (AGDAP) in two body positions according to the methods by 'The Infant Development and the Environment Study' (TIDES) and 'Cambridge Baby Growth Study', resulting in four mean values per patient (TIDES AGDAS/AP and Cambridge AGDAS/AP). Normal values for AGD by age were set by our hospital Department of Growth and Reproduction based on a large cohort of healthy infant boys (n = 1940). Testicular biopsies were performed at orchidopexy as a clinical routine. The germ cell number (G/T) and type Ad spermatogonia number (AdS/T) per cross-sectional tubule of at least 100 and 250 tubules, respectively were measured and related to normal samples. Blood samples were obtained by venipuncture for measuring serum LH, FSH, and inhibin B. They were analyzed in our hospital Department of Growth and Reproduction where the normal reference was also established. Correlations between the four mean AGD measurements for each boy were evaluated by Spearman rank correlation analyses. The AGD measurement of every boy was transferred to the multiple of the median (MoM) of the normal AGD for age and named MoM AGD.
MAIN RESULTS AND THE ROLE OF CHANCE
There were 104 boysoperated for unilateral, and 47 boys operated for bilateral, undescended testes, whereas 18 boys had vanished testis including one boy with bilateral vanished testes. Only 6% of cases with vanished testes had a MoM AGD higher than the normal median compared to 32% with undescended testes (P < 0.05). MoM AGD increased with the age at surgery for boys with vanished testis (Spearman r = 0.44), but not for boys with undescended testes (Spearman r = 0.14). Boys with bilateral cryptorchidism had longer AGDs and more often had hypogonadotropic hypogonadism than boys with unilateral cryptorchidism (P < 0.005) and (P < 0.000001).
LIMITATIONS, REASONS FOR CAUTION
Although being the largest published material of AGD measurements of infant boys with cryptorchidism, one limitation of this study covers the quite small number of patients in the different groups, which may decrease the statistical power. Another limitation involves the sparse normal reference material on G/T and AdS/T. Finally, there are currently no longitudinal studies evaluating AGD from birth to adulthood and evaluating childhood AGD in relation to fertility outcome. Our study is hypothesis generating and therefore the interpretation of the results should be regarded as exploratory rather than reaching definite conclusions.
WIDER IMPLICATIONS OF THE FINDINGS
The study findings are in agreement with literature as the total included group of boys with cryptorchidism exhibited shorter than normal AGDs. However, new insights were demonstrated. Boys with vanished testis had shorter AGDs compared to unaffected boys and to boys with undescended testes. This finding challenges the current concept of AGD being determined in 'the masculinization programming window' in Week 8 to 14 of gestation. Furthermore, boys with bilateral cryptorchidism had longer AGDs and more often had hypogonadotropic hypogonadism than boys with unilateral cryptorchidism, suggesting that the lack of fetal androgen in hypogonadotropic hypogonadism is not that significant.
STUDY FUNDING/COMPETING INTEREST(S)
No external funding was used and no competing interests are declared.
TRIAL REGISTRATION NUMBER
The trial was not registered in an ICMJE-recognized trial registry.
Topics: Male; Pregnancy; Infant; Female; Child; Humans; Cryptorchidism; Testicular Neoplasms; Androgens; Semen Analysis; Cohort Studies; Cross-Sectional Studies; Prospective Studies; Hypogonadism; Gonadal Dysgenesis, 46,XY; Testis
PubMed: 38373213
DOI: 10.1093/humrep/deae025 -
Phytomedicine : International Journal... Mar 2024In our previous study, we provided evidence that Astragalus mongholicus Bunge(AM) and its extracts possess a protective capability against radiation-induced damage,...
BACKGROUND
In our previous study, we provided evidence that Astragalus mongholicus Bunge(AM) and its extracts possess a protective capability against radiation-induced damage, potentially mediated through the reduction of reactive oxygen species (ROS) and nitric oxide (NO). However, we were pleasantly surprised to discover during our experimentation that AM not only offers protection against radiation damage but also exhibits a radiation sensitization effect. This effect may be attributed to a specific small molecule present in AM known as ononin. Currently, radiation sensitizers are predominantly found in nitrazole drugs and nanomaterials, with no existing reports on the radiation sensitization properties of ononin, nor its underlying mechanism.
PURPOSE
This study aims to investigate the sensitization effect of the small molecule ononin derived from AM on lung cancer radiotherapy, elucidating its specific molecular mechanism of action. Additionally, the safety profile of combining astragalus small molecule ononin with radiation therapy will be evaluated.
METHODS
The effective concentration of ononin was determined through cell survival experiments, and the impact of ononin combined with varying doses of radiation on lung cancer cells was observed using CCK-8 and cell cloning experiments. The apoptotic effect of ononin combined with radiation on lung cancer cells was assessed using Hochester staining, flow cytometry, and WB assay. Additionally, WB and immunofluorescence analysis were conducted to investigate the influence of ononin on HIF-1α/VEGF pathway. Furthermore, Molecular Dynamics Simulation was employed to validate the targeted binding ability of ononin and HIF-1α. A lung cancer cell line was established to investigate the effects of knockdown and overexpression of HIF-1α. Subsequently, the experiment was repeated using tumor bearing nude mice and C57BL/6 mouse models in an in vivo study. Tumor volume was measured using a vernier caliper, while HE, immunohistochemistry, and immunofluorescence techniques were employed to observe the effects of ononin combined with radiation on tumor morphology, proliferation, and apoptosis. Additionally, Immunofluorescence was employed to examine the impact of ononin on HIF-1α/VEGF pathway in vivo, and its effect on liver function in mice was assessed through biochemistry analysis.
RESULTS
At a concentration of 25 μM, ononin did not affect the proliferation of lung epithelial cells but inhibited the survival of lung cancer cells. In vitro experiments demonstrated that the combination of ononin and radiation could effectively inhibit the growth of lung cancer cells, induce apoptosis, and suppress the excessive activation of the Hypoxia inducible factor 1 alpha/Vascular endothelial growth factor pathway. In vivo experiments showed that the combination of ononin and radiation reduced the size and proliferation of lung cancer tumors, promoted cancer cell apoptosis, mitigated abnormal activation of the Hypoxia inducible factor 1 alpha pathway, and protected against liver function damage.
CONCLUSION
This study provides evidence that the combination of AM and its small molecule ononin can enhance the sensitivity of lung cancer to radiation. Additionally, it has been observed that this combination can specifically target HIF-1α and exert its effects. Notably, ononin exhibits the unique ability to protect liver function from damage while simultaneously enhancing the tumor-killing effects of radiation, thereby demonstrating a synergistic and detoxifying role in tumor radiotherapy. These findings contribute to the establishment of a solid basis for the development of novel radiation sensitizers derived from traditional Chinese medicine.
Topics: Mice; Animals; Lung Neoplasms; Vascular Endothelial Growth Factor A; Mice, Nude; Cell Line, Tumor; Mice, Inbred C57BL; Vascular Endothelial Growth Factors; Radiation Tolerance; Radiation-Sensitizing Agents; Hypoxia-Inducible Factor 1; Hypoxia-Inducible Factor 1, alpha Subunit; Glucosides; Isoflavones
PubMed: 38308918
DOI: 10.1016/j.phymed.2023.155290 -
BMC Veterinary Research Feb 2024The aim of this study was to introduce a novel intraoral technique for performing mandibular nerve blocks in dromedary camels (Camelus dromedarius). In this study, 18...
The aim of this study was to introduce a novel intraoral technique for performing mandibular nerve blocks in dromedary camels (Camelus dromedarius). In this study, 18 adult camel skulls of varying ages and breeds were examined to determine the position of the mandibular foramen. Using a Vernier caliper, three dimensions in millimeters were measured: (1) the distance between the mandibular foramen (MF) and the caudal edge of the third molar tooth at the occlusal surface level, (2) the distance between the MF and the rostral edge border of the mandible's ramus (RER) at the occlusal surface level, and (3) the distance between the MF and the ventral margin border of the mandible (VM). The technique was evaluated using five intact camel cadaver heads (n = 5), and a total of ten mandibular nerve blocks were described. An 18-gauge 80-mm Tuohy needle was inserted into the mouth commissure and advanced caudally while injecting a saline-methylene blue solution. The accuracy of the injection was confirmed through the infiltration of the contrast dye into the target area using computed tomography (CT) and post procedural dissection. Anatomical study of the mandibular nerve site was performed to aid the blind insertion of the needle. The findings contribute to the development of veterinary anesthesia techniques and provide anatomical considerations for clinicians performing oral surgeries in sedated camels. The results demonstrated the successful implementation of the intraoral technique, highlighting its efficacy and reliability in achieving local anesthesia for oral surgeries involving the lower jaw and teeth in sedated camels. Further research studies are needed to evaluate the long-term efficacy and safety of the technique and to compare it with existing approaches.
Topics: Animals; Camelus; Reproducibility of Results; Nerve Block; Mandible; Tomography, X-Ray Computed; Mandibular Nerve; Analgesia; Cadaver
PubMed: 38308261
DOI: 10.1186/s12917-024-03885-4