-
Diagnostics (Basel, Switzerland) Feb 2023The objective of this research is to evaluate cervical regeneration after large loop excision of the transformation zone (LLETZ) through the identification of a new...
The objective of this research is to evaluate cervical regeneration after large loop excision of the transformation zone (LLETZ) through the identification of a new sonographic reference point at the level of the uterine margins. In the period March 2021-January 2022, a total of 42 patients affected by CIN 2-3 were treated with LLETZ at the University Hospital of Bari (Italy). Before performing LLETZ, cervical length and volume were measured with trans-vaginal 3D ultrasound. From the multiplanar images, the cervical volume was obtained using the Virtual Organ Computer-aided AnaLysis (VOCAL™) program with manual contour mode. The line that connects the points where the common trunk of the uterine arteries reaches the uterus splitting into the ascending major branch and the cervical branch was considered as the upper limit of the cervical canal. From the acquired 3D volume, the length and the volume of the cervix were measured between this line and the external uterine os. Immediately after LLETZ, the removed cone was measured using Vernier's caliper, and before fixation in formalin, the volume of the excised tissue was evaluated by the fluid displacement technique based on the Archimedes principle. The proportion of excised cervical volume was 25.50 ± 17.43%. The volume and the height of the excised cone were 1.61 ± 0.82 mL and 9.65 ± 2.49 mm corresponding to 14.74 ± 11.91% and 36.26 ± 15.49% of baseline values, respectively. The volume and length of the residual cervix were also assessed using 3D ultrasound up to the sixth month after excision. At 6 weeks, about 50% of cases reported an unchanged or lower cervical volume compared to the baseline pre-LLETZ values. The average percentage of volume regeneration in examined patients was equal to 9.77 ± 55.33%. In the same period, the cervical length regeneration rate was 69.41 ± 14.8%. Three months after LLETZ, a volume regeneration rate of 41.36 ± 28.31% was found. For the length, an average regeneration rate of 82.48 ± 15.25% was calculated. Finally, at 6 months, the percentage of regeneration of the excised volume was 90.99 ± 34.91%. The regrowth percentage of the cervical length was 91.07 ± 8.03%. The cervix measurement technique that we have proposed has the advantage of identifying an unequivocal reference point in 3D cervical measurement. Ultrasound 3D evaluation could be useful in the clinical practice to evaluate the cervical tissue deficit and express the "potential of cervical regeneration" as well as provide the surgeon useful information about the cervical length.
PubMed: 36832279
DOI: 10.3390/diagnostics13040791 -
Journal of Functional Biomaterials Feb 2023To evaluate the effectiveness of hyaluronic acid (HA) gel injection with and without plasma rich in growth factors (PRGF) for the management of interdental papillary...
BACKGROUND
To evaluate the effectiveness of hyaluronic acid (HA) gel injection with and without plasma rich in growth factors (PRGF) for the management of interdental papillary loss.
METHODS
A single blinded randomized clinical trial was carried out on 21 subjects with 34 sites. Patients within the age group 18-45 years who had Class I and II papillary recession in the maxillary anterior region were selected. The sites involved were randomly assigned to Group HA alone and Group HA + PRGF. The patients were recalled 4 weeks after receiving supragingival and subgingival instrumentation. HA or HA + PRGF was injected into the defective papilla at baseline and at 3 and 6 weeks. Image based measurements of Papillary Width (PW), Papillary Deficient Height (PDH), Deficient Area (DA), Deficient Volume (DV) were registered at baseline, 3 weeks, 6 weeks and 12 weeks. A vernier caliper was used to measure the papillary depth in the impression made using additional silicone impression material pre- and post-intervention.
RESULTS
There was a significant improvement in the within-group comparison of PW, PDH, DA and DV in both the groups. Group HA + PRGF showed significantly greater improvement in comparison to Group HA alone in terms of PDH, DA and DV at 6 and 12 weeks.
CONCLUSIONS
Even though HA gel has already been established as a promising injectable agent in the minimally invasive treatment of interdental papillary deficiency, PRGF may also have a significant adjuvant effect when used along with HA. Further clinical studies with longer follow up duration, larger sample size and standardization of the tooth shape are required for a better understanding of the adjuvant effect of PRGF when used along with HA.
PubMed: 36826913
DOI: 10.3390/jfb14020114 -
Cureus Jan 2023Anthropometric measurements of the distal femoral fragment play a crucial role in prosthesis design during arthroplastic knee surgeries and offer valuable clues for...
INTRODUCTION
Anthropometric measurements of the distal femoral fragment play a crucial role in prosthesis design during arthroplastic knee surgeries and offer valuable clues for stature estimation in forensic investigations. The present study is an attempt to assess various anthropometric parameters of the distal femur in this regard.
MATERIALS AND METHODS
A total of 96 intact dry femora were anthropometrically assessed using digital Vernier calipers. The femoral length was measured using an osteometric table. The torsion angle was calculated with an analog goniometer. The various parameters studied included: medial condyle length and thickness, lateral condyle length and thickness, bicondylar width, intercondylar width, intercondylar depth, torsional angle, and femoral length. The data obtained were statistically analyzed using SPSS software (IBM Corp., Armonk, NY, USA).
RESULTS
Mean medial condyle length was 57.38±4.47mm and thickness was 24.53±2.27mm. Mean lateral condyle length and thickness were found to be 58.49±4.3mm and 25.33±3.15mm respectively. Mean bicondylar width was 71.96±6.73mm, mean intercondylar width 21.86±2.71mm, and the intercondylar depth 27.04±2.59mm respectively. The average femur length was 41.87±3.31mm and the average torsion angle was 20.19°±6.99°. Significant correlations were observed between distal femoral parameters. Lateral condyle length showed maximum correlation with other parameters. Femur length was found to correlate significantly with all parameters except medial condyle thickness. Torsion angle was significantly correlated with lateral condyle length and femur length only.
DISCUSSION
The findings of this study show considerable variation from those of other studies done within India. This proves that distal femoral anthropometry has regional variations. These data can aid sports physicians and orthopedic surgeons with implant designing and forensic experts during investigations.
PubMed: 36820105
DOI: 10.7759/cureus.33945 -
Current Medical Imaging 2023This study proposes a method for improving the accuracy of three-dimensional (3D) models generated through cone-beam computed tomography (CBCT).
PURPOSE
This study proposes a method for improving the accuracy of three-dimensional (3D) models generated through cone-beam computed tomography (CBCT).
METHODS
A 3D cuboid model fitted with a ¼-scale dentition on its top surface was constructed to simulate an alveolar bone with teeth. A physical specimen of the model was printed and the distance between its opposite sides was measured using a vernier caliper. The physical model was light-scanned, and the surface data of the generated 3D model were corrected by calibrating the distance between opposite sides against the vernier caliper measurements. The physical model was also scanned using CBCT to reconstruct a second 3D model. The overall deviation between the two models and the distance deviation in each direction of the cuboid and dentition were quantified and statistically analyzed.
RESULTS
The overall deviation between the reconstructed CBCT model and the calibrated structured light-scanned model was 0.098 ± 0.001 mm. Following calibration, the overall deviation was 0.010 ± 0.006 mm. A one-way variance analysis suggested that the overall deviations' differences were not statistically significant (P < 0.05).
CONCLUSION
This study lays a solid foundation for accurate dental implantation.
Topics: Humans; Imaging, Three-Dimensional; Calibration; Spiral Cone-Beam Computed Tomography; Cone-Beam Computed Tomography
PubMed: 36799416
DOI: 10.2174/1573405619666230217121745 -
JNMA; Journal of the Nepal Medical... Oct 2022The measurement of the teeth is one of the most reliable method of identification. The teeth represent the most durable, resilient, and chemically stable part of the...
INTRODUCTION
The measurement of the teeth is one of the most reliable method of identification. The teeth represent the most durable, resilient, and chemically stable part of the skeleton. The comparison of tooth dimensions is one of the tools of sex determination. Canines, in particular, are found to have the greatest degree of sexual dimorphism. The aim of the study was to determine the mean mesiodistal width of canines in patients visiting a tertiary care centre.
METHODS
This descriptive cross-sectional study was done among patients visiting a tertiary care centre from 25 August 2021 to 28 December 2021. The ethical approval was taken from the Institutional Review Committee [Reference number: 61 (6-11)E2 078/079]. The maximum mesiodistal width of the permanent maxillary canine was measured by a digital vernier caliper. The formula given by Garn and Len was used to calculate sexual dimorphism. Convenience sampling was used. Point estimate and 95% Confidence Interval were calculated.
RESULTS
Among 104 maxillary casts studied, the mean mesiodistal width of the maxillary canine was 7.85±0.45 mm (7.76-7.93, 95% Confidence Interval). The mean mesiodistal width of the right maxillary canine in males and females were 7.90±0.48 mm and 7.83±0.45 mm respectively and that of the left maxillary canines in males and females were 7.92±0.44 mm and 7.75±0.45 mm respectively. The sexual dimorphism in the right and left maxillary canine was seen in 10 (0.96%) and 22 (2.12%) respectively.
CONCLUSIONS
The mean mesiodistal width of the maxillary canine was similar to other studies done in similar settings.
KEYWORDS
canine teeth; maxillary bone; sexual dimorphism.
Topics: Male; Female; Humans; Cross-Sectional Studies; Tertiary Care Centers; Cuspid; Maxilla; Sex Characteristics
PubMed: 36705164
DOI: 10.31729/jnma.7099 -
Journal of Microscopy and Ultrastructure 2022The meniscal cartilages are fibrous discs that are important for knee structures and have the ability to bear weight and stabilize joints. However, morphological and...
BACKGROUND
The meniscal cartilages are fibrous discs that are important for knee structures and have the ability to bear weight and stabilize joints. However, morphological and standard data for the meniscus are limited. Therefore, this work will compare anatomical and histological parameters of meniscal cartilages. The results will be important for the different measurements that are necessary for knee joint surgery.
MATERIALS AND METHODS
A total of 24 aged cadavers (12 males and 12 females) were included. Knee joints were dissected and the menisci were excised and labeled as medial or lateral, right or left, male or female. Then, the menisci were kept in 10% formalin solution. Morphological variations of the meniscal shapes were macroscopically categorized. Different measurements, including the distance between anterior and posterior horns, outer and inner circumferences, width (breadth), and thickness, were done using a digital Vernier caliper and recorded manually.
RESULTS
48 medial menisci (MMi) cartilages were studied, they were 54.6% crescent-shaped, 34.6% V-shaped, and 10.8% U-shaped. 48 lateral menisci (LMi) cartilages were studied, 41.6% were crescent-shaped, 56.4% were C-shaped, and 2% were disc-shaped articular cartilage. Findings included differences in their lengths and thickness.
CONCLUSION
The findings of this study were significant in providing new information on various morphological and morphometric parameters of the MMi and LMi in aged males and females, which are necessary to require more precise and comprehensive fundamental data that will be helpful for many specialists for better diagnostic and therapeutic approaches; aiming to restore normal joint conditions in senile people complaining of different meniscal pathologies.
PubMed: 36687326
DOI: 10.4103/jmau.jmau_91_21 -
Frontiers in Neuroscience 2022Obtaining accurate axial length (AL) is very important for the establishment of animal models of myopia. The purpose of this study is to compare the accuracy of Quantel...
BACKGROUND
Obtaining accurate axial length (AL) is very important for the establishment of animal models of myopia. The purpose of this study is to compare the accuracy of Quantel A-B scan, OD-1 A scan, and vernier caliper in measuring AL in Sprague Dawley (SD) rats.
METHODS
In total, 60 5-week-old SD rats were divided into female rat group ( = 30) and male rat group ( = 30). Quantel A-B scan and OD-1 A scan were, respectively, used to measure the AL of both eyes of each living rat, and vernier caliper was used to measure the anterior-posterior diameter of each rat's eyeball. Besides, the correlation between refractive error (RE) and AL measured by different instruments was evaluated, and the accuracy of the three measurement methods was compared according to gender and left/right eyes.
RESULTS
There were significant differences in AL and diopter of SD rats at the same age ( < 0.05). the AL of male rats was greater than that of female rats, while diopter (D) was the opposite; There was no significant difference in AL and D between left and right eyes in the same SD rats ( > 0.05); There were statistical differences among the three measurement methods ( < 0.05), AL measured by vernier caliper was the largest, followed by Quantel A-B scan, OD-1 A scan; Difference in AL between male and female was not statistically significant between the results obtained by Quantel A-B scan and vernier caliper ( > 0.05), but there were statistically significant differences between the other two measurement methods ( < 0.05).
CONCLUSION
Sex is the influencing factor of AL and RE. Imaging measurement can accurately measure the AL in living small rodents. Compared with OD-1 A scan, Quantel A-B scan may be more accurate.
PubMed: 36685229
DOI: 10.3389/fnins.2022.1106904 -
F1000Research 2022In the issue of biodiversity, the domestication of birds as pets and trade animals requires special attention as a conservation effort. Lovebirds ( ) are popular birds...
BACKGROUND
In the issue of biodiversity, the domestication of birds as pets and trade animals requires special attention as a conservation effort. Lovebirds ( ) are popular birds worldwide, due to their varied ornamentation and melodic chirping sound. Syrinx structure is suspected to be the main source of sound production during the chirping period. This study aimed to investigate syrinx morphometry and its correlation with sound frequency produced in lovebirds.
METHODS
A total of 24 lovebirds of different ages and gender were investigated. Polymerase chain reaction method was performed to determine lovebird gender, meanwhile bird age was identified based on post-hatch recordings at the breeding farm. Thus, we enrolled male (n=12) and female (n=12) lovebirds aged 2 (n=4), 3 (n=4), and 4 (n=4) months in the investigation group, respectively. Fast Fourier Transform (FFT) was performed to evaluate sound frequency during chirping period. Then, syrinx morphometry was identified using a topographic approach and methylene blue staining. Each variable was evaluated with Image J software and vernier caliper.
RESULTS
Based on a topographical approach, we reported the general cartilage structure of the tracheosyringeal, bronchosyringeal, paired protrusions, tracheolateral muscles, sternotracheal muscles, and syringeal muscles in lovebird syrinx. In particular, the tympaniform membranes lateral lead a crucial role in modulating the frequency of male lovebirds more significantly (p=0,009) compared to female. On the other hand, the tympaniform membranes lateral dexter (p=0,02) and sinister (p=0,05) in females showed wider compared to male. We also reported a negative correlation between sound frequency compared to tympaniform membranes lateral dexter (y = -913,56x + 6770,8) and sinister (y = -706,16x + 5736).
CONCLUSIONS
It can be concluded that the tympaniform membranes lateral produced the lovebirds' primary sound. The sound frequency of male lovebirds was higher compared to female, however negatively correlated with the area of tympaniform membranes lateral.
PubMed: 38779459
DOI: 10.12688/f1000research.108884.2 -
Journal of Advanced Pharmaceutical... Nov 2022Stature is the height of an individual in an upright position. It is one of the key characteristics, which helps in defining any individual. Stature estimation is needed...
Stature is the height of an individual in an upright position. It is one of the key characteristics, which helps in defining any individual. Stature estimation is needed mostly in medicolegal cases where only a limited number of human fragments are left for forensic analysis. The teeth provide essential measurements that can be used to determine stature. The aim of the study is to find out whether the second maxillary interpremolar distance can be used to effectively estimate stature of individuals. For the study, the sample size taken was 60 (30 male and 30 females), and their second maxillary interpremolar distance was measured using a digital vernier caliper. The collected data were tabulated and statistical analysis was done using SPSS software (23), followed by linear regression. For male: Y = 178.65 - 1.09X, correlation coefficient, = 0.05, For female: Y = 169.30 - 1.99X, correlation coefficient, = 0.13. Since < 0.2, there is no correlation between the interpremolar distance of the second maxillary premolar and the stature. From our study, we conclude that the interpremolar distance of the second maxillary premolar cannot be a reliable parameter for stature estimation in both genders.
PubMed: 36643103
DOI: 10.4103/japtr.japtr_216_22 -
Morphometric analysis of Bonwill's triangle and its dental applications in dry human mandible bones.Journal of Advanced Pharmaceutical... Nov 2022Bonwill's triangle is an imaginary equilateral triangle formed when the centers of two condyles and each condyle with the medial mandibular incisal midpoint are joined....
Bonwill's triangle is an imaginary equilateral triangle formed when the centers of two condyles and each condyle with the medial mandibular incisal midpoint are joined. The dimensions of the tooth, with other bones of cranium, and the entire anatomical structure of body are in persistent association with the distance of the borders of the triangle. It can be used to study articulation of the mandible, complete dentures, and dental occlusion and can be related when treating mandibular fractures. The aim is to analyze the variations in the length of Bonwill's triangle in dry human mandibles and its dental implications. Forty dry human mandibles were taken to carry out this study. Distances between the center of the right condylar process to the inner medial mandibular incisal midpoint (A), the distance between the center of the left condylar process to the inner medial mandibular incisal midpoint (B), and the distance between the centers of right and left condylar process of mandible (C) were measured with the aid of digital vernier caliper. The statistics was evaluated and observed using the statistical analysis software SPSS (Version 20.0) and the mean and standard deviation was calculated. The mean length between right condyle center and medial mandibular incisal midpoint is 97.76 mm, between left condyle center and medial mandibular incisal midpoint is 98.55 mm, and between the right and left condyle centers is 97.39 mm. The values are almost equal to 4 inches. The mandibular measurements serve as an important factor for many clinical conditions especially related to dentistry. The results of this study can be of great significance when treating mandibular fractures and defects.
PubMed: 36643100
DOI: 10.4103/japtr.japtr_151_22