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Chemosphere Jan 2024The worldwide and intensive use of phytosanitary compounds results in environmental and food contamination by chemical residues. Human exposure to multiple pesticide...
Low concentrations of ethylene bisdithiocarbamate pesticides maneb and mancozeb impair manganese and zinc homeostasis to induce oxidative stress and caspase-dependent apoptosis in human hepatocytes.
The worldwide and intensive use of phytosanitary compounds results in environmental and food contamination by chemical residues. Human exposure to multiple pesticide residues is a major health issue. Considering that the liver is not only the main organ for metabolizing pesticides but also a major target of toxicities induced by xenobiotics, we studied the effects of a mixture of 7 pesticides (chlorpyrifos-ethyl, dimethoate, diazinon, iprodione, imazalil, maneb, mancozeb) often detected in food samples. Effects of the mixture was investigated using metabolically competent HepaRG cells and human hepatocytes in primary culture. We report the strong cytotoxicity of the pesticide mixture towards hepatocytes-like HepaRG cells and human hepatocytes upon acute and chronic exposures at low concentrations extrapolated from the Acceptable Daily Intake (ADI) of each compound. Unexpectedly, we demonstrated that the manganese (Mn)-containing dithiocarbamates (DTCs) maneb and mancozeb were solely responsible for the cytotoxicity induced by the mixture. The mechanism of cell death involved the induction of oxidative stress, which led to cell death by intrinsic apoptosis involving caspases 3 and 9. Importantly, this cytotoxic effect was found only in cells metabolizing these pesticides. Herein, we unveil a novel mechanism of toxicity of the Mn-containing DTCs maneb and mancozeb through their metabolization in hepatocytes generating the main metabolite ethylene thiourea (ETU) and the release of Mn leading to intracellular Mn overload and depletion in zinc (Zn). Alteration of the Mn and Zn homeostasis provokes the oxidative stress and the induction of apoptosis, which can be prevented by Zn supplementation. Our data demonstrate the hepatotoxicity of Mn-containing fungicides at very low doses and unveil their adverse effect in disrupting Mn and Zn homeostasis and triggering oxidative stress in human hepatocytes.
Topics: Humans; Maneb; Manganese; Pesticides; Zineb; Fungicides, Industrial; Apoptosis; Oxidative Stress; Zinc; Hepatocytes; Ethylenes; Homeostasis
PubMed: 37923018
DOI: 10.1016/j.chemosphere.2023.140535 -
Analytical and Bioanalytical Chemistry Dec 2023A novel deep learning-enabled smartphone platform is developed to assist a colorimetric aptamer biosensor for fast and highly sensitive detection of dimethoate. The...
A novel deep learning-enabled smartphone platform is developed to assist a colorimetric aptamer biosensor for fast and highly sensitive detection of dimethoate. The colorimetric determination of dimethoate is based on the specific binding of dimethoate and aptamer, which leads to the aggregation of AuNPs in high-concentration NaCl solution, resulting in an obvious color change from red to blue. This color change provides sufficient data for self-learning enabled by a convolutional neural network (CNN) model, which is established to predict dimethoate concentration based on images acquired from a smartphone. To enhance user-friendliness for non-experts, the CNN model is then embedded into a smartphone app, enabling offline detection of dimethoate pesticide in real environments within just 15 min using a pre-configured colorimetric probe. The developed platform exhibits superior performance, achieving a regression coefficient of 0.9992 in the concentration range of 0-10 μM. Moreover, the app's performance is found to be consistent with the ELISA kit. These remarkable findings demonstrate the potential of combining colorimetric biosensors with smartphone-based deep learning methods for the development of portable and affordable tools for pesticide detection.
Topics: Colorimetry; Dimethoate; Pesticides; Smartphone; Metal Nanoparticles; Gold; Deep Learning; Limit of Detection; Aptamers, Nucleotide; Biosensing Techniques
PubMed: 37770666
DOI: 10.1007/s00216-023-04978-z -
Environmental Science and Pollution... Oct 2023In this work, metal-organic frameworks (MOFs) including Fe-MIL-101 and Ti-MIL-125 were prepared and fixed on the melamine foam (MF) by polyvinylidene fluoride (PVDF) to...
In this work, metal-organic frameworks (MOFs) including Fe-MIL-101 and Ti-MIL-125 were prepared and fixed on the melamine foam (MF) by polyvinylidene fluoride (PVDF) to prepare MF/PVDF/MOFs, which was used as adsorbents in pipette-tip solid-phase extraction (PT-SPE) for rapid extraction of organophosphorus pesticides (OPPs). Then, a gas chromatograph-flame thermionic detector (GC-FTD) was used for simultaneous analysis of Dimethoate (DMT), Iprobenfos (IBF), Parathion-methyl (PAM), and Chlorpyrifos (CPF). The morphology, crystal structure, and functional groups of MF/PVDF/MOFs were characterized, indicating that Ti-MIL-125 and Fe-MIL-101 were successfully synthesized and distributed on MF. The Fe-MIL-101 and Ti-MIL-125 showed good extraction ability for OPPs, which was mainly due to the π-π interaction and the multiple porous structures. Under the optimal conditions, the limit of detection (LODs) of four OPPs was 0.03-0.14 μg L and the RSDs were less than 9.9%. The developed PT-SPE method showed a short extraction time (<3 min). The recoveries in fruits and vegetables (Celery, cabbages, and oranges) ranged from 75.3%-118.8% (RSDs<9.6%). The prepared MF/PVDF/MOFs demonstrated the efficient extraction performance of OPPs, contributing to the rapid pretreatment of OPPs from food and the environment.
Topics: Pesticides; Metal-Organic Frameworks; Organophosphorus Compounds; Vegetables; Fruit; Solid Phase Extraction; Limit of Detection
PubMed: 37755595
DOI: 10.1007/s11356-023-30055-0 -
Gene Jan 2024The insecticide dimethoate, an organophosphate, has been used on crops, soybeans, fruits, and vegetables since the 1960s and is considered one of the most widely used...
The insecticide dimethoate, an organophosphate, has been used on crops, soybeans, fruits, and vegetables since the 1960s and is considered one of the most widely used pesticides. However, the understanding of the molecular mechanisms of dimethoate in crops, especially crop seedlings, is still limited. The green vegetable soya bean (Glycine max merr) is usually used as a vegetable-like fruit of soybean in many Asian countries. This study aimed to analyze the effect of dimethoate on the growth of green vegetable soya bean seedlings at the metabolic and transcriptional levels. An integrated analysis of the transcriptome and metabolome was performed to determine the responses of green vegetable soya bean seedlings to different concentrations (D1 for low dose, D2 for high dose and C for control) of dimethoate. In omics analyses, 4156 differentially expressed genes (DEGs) and 1935 differentially abundant metabolites (DAMs) were identified in the D1/C comparison, and 11,162 DEGs and 819 DAMs were identified in D2/C. Correlation analyses revealed dimethoate affected the metabolic pathways of green vegetable soya beans such as the biosynthesis of secondary metabolites and microbial metabolism in diverse environmental pathways, demonstrating that even small doses of dimethoate can affect green vegetable soya bean seedlings in a short period of time. Our study further enriches our understanding of the molecular mechanisms by which green vegetable soya beans are treated with dimethoate and provides a deeper understanding of the effects of dimethoate on crops.
Topics: Glycine max; Vegetables; Dimethoate; Transcriptome; Seedlings
PubMed: 37739194
DOI: 10.1016/j.gene.2023.147799 -
Anais Da Academia Brasileira de Ciencias 2023Although it is known that organophosphate insecticides are harmfull to aquatic ecosystems, oxidative damages caused by Dimethoate and Chlorpyrifos are not studied on...
Although it is known that organophosphate insecticides are harmfull to aquatic ecosystems, oxidative damages caused by Dimethoate and Chlorpyrifos are not studied on Arthrospira platensis Gomont. In this study, various Chlorpyrifos (0-150 µg mL-1) and Dimethoate (0-250 µg mL-1) concentrations were added to the culture medium in laboratory to evaulate growth rate, chlorophyll-a content and antioxidant parameters of A. platensis. Optical Density (OD560) and chlorophyll-a decreased compared to the control for seven days in both pesticide applications. Superoxide dismutase (SOD) activity increased at 50 µg mL-1 Chlorpyrifos concentration but it decreased at all concentrations. Although Ascorbate peroxidase (APX) and glutathione reductase (GR) activities increased with Chlorpyrifos application, they did not change with Dimethoate application. Malondialdehyde (MDA) amount decreased at 150 µg mL-1 Chlorpyrifos concentration but it increased in Dimethoate application. The H2O2 content were increased in both applications. Proline decreased in 50 and 75 µg mL-1 Chlorpyrifos concentrations and increased at 150 µg mL-1 concentration, while it increased at 25 µg mL-1 Dimethoate concentration. The results were tested at 0.05 significance level. These pesticides inhibit A. platensis growth and chlorophyll-a production and cause oxidative stress. The excessive use may affect the phytoplankton and have negative consequences in the aquatic ecosystem.
Topics: Insecticides; Chlorpyrifos; Dimethoate; Ecosystem; Hydrogen Peroxide; Oxidative Stress; Pesticides; Antioxidants; Chlorophyll; Chlorophyll A; Organophosphorus Compounds
PubMed: 37729300
DOI: 10.1590/0001-3765202320200463 -
Journal of Environmental Management Nov 2023Natural soil components, such as clays, have recently piqued interest because of their potential as pesticide adsorbents. This research work sheds light on the...
Natural soil components, such as clays, have recently piqued interest because of their potential as pesticide adsorbents. This research work sheds light on the possibility of the application of natural Albanian clays as adsorbents for methomyl and dimethoate pesticides from aqueous solutions. Natural Albanian clays from the regions of Brari, Currila, Dardha, and Prrenjasi were employed in the study and were characterized by granulometric analysis and powder X-ray diffraction. Each clay's adsorption capacity and desorption behavior were investigated toward the chosen pesticides. Within 48 h of contact time, methomyl and dimethoate solutions with different concentrations were evaluated at 25 °C to see how the insecticide concentration affected the adsorption & desorption processes for each natural clay type. The experimental data were fitted to Freundlich, Temkin and Dubinin-Radushkevich isotherm like functions and the results showed the best correlation on Freundlich like adsorption isotherm for almost all cases. Brari clay performed better adsorptive properties toward dimethoate, followed by Dardha, Currila and Prrenjasi clays. The dimethoate adsorbed quantities varied from 0.250 mg/g for C = 0.200 g/L to 0.822 mg/g for C = 0.500 g/L. In comparison to Dardha and Prrenjasi clays, Brari and Currila clays exhibit longer saturation times and improved methomyl retention. In the first 2 h of contact, 96.5% of methomyl and 81% of dimethoate were desorbed from Brari clay. The adsorption process was also investigated employing pseudo first-order and pseudo second-order kinetic models, with the results indicating that all clay-pesticide systems studied demonstrated second-order kinetic behavior. Based on the studied desorption process, it is possible to impregnate clays with various insecticides in agriculture and completely control the quantities of the insecticide released.
Topics: Clay; Pesticides; Methomyl; Dimethoate; Insecticides; Adsorption; Kinetics
PubMed: 37717393
DOI: 10.1016/j.jenvman.2023.118989 -
Toxicology Reports Dec 2023This paper reviewed the published data on the levels of different pesticide residues in vegetables (tomato, eggplant, beans, gourds, cauliflower, cabbage, cucumber,... (Review)
Review
This paper reviewed the published data on the levels of different pesticide residues in vegetables (tomato, eggplant, beans, gourds, cauliflower, cabbage, cucumber, potato, carrot, onion, red chilli, red amaranth, lady's finger, spinach, coriander, and lettuce) from Bangladesh in the last decade. Vegetable production in Bangladesh has increased tremendously (37.63%) compared to the last decades, along with its pesticide use. The most observed pesticide groups used in vegetable production were organophosphorus, pyrethroids, carbamate, organochlorine, nereistoxin analogue group, and neonicotinoids. More specifically, chlorpyrifos, dimethoate, diazinon, and malathion were the most used pesticides. More than 29% of the vegetable samples (1577) were contaminated with pesticide residue; among the contaminated samples (458), most cases (73%) exceeded the maximum residue limits (MRLs). The pesticide-contaminated vegetables were cucumber (51%), tomato (41%), cauliflower (31%), miscellaneous vegetables (36%), eggplant (29%), beans (23%), cabbage (18%), and gourds (16%). Among the pesticide-contaminated samples, vegetables with above MRL were gourds (100%), beans (92), tomato (78%), eggplant (73%), miscellaneous vegetables (69%), cucumber (62%), cabbage (50%), cauliflower (50%) (p < 0.05). It was also observed that a single vegetable was often contaminated with multiple pesticides, and farmers did not follow a proper withdrawal period while using pesticides. Hazard quotation (HQ>1) was observed in adolescents and adults in tomato, eggplant, beans, cauliflower, cabbage, cucumber, lady's finger, lettuce, and coriander. There was no health risk observed (HQ<1) in gourds, potato, carrot, onion, red chilli, red amaranth, spinach, and okra. The highest acute and chronic HQ (aHQ, cHQ) was observed for cypermethrin (bean) in adolescents (aHQ=255, cHQ= 510) and adults (aHQ=131, cHQ=263). It was also observed that these pesticides harmed air, soil, water, and non-target organisms. Nevertheless, the review will help the government develop policies that reduce pesticide use and raise people's awareness of its harmful effects.
PubMed: 37711360
DOI: 10.1016/j.toxrep.2023.09.003 -
Environmental Research Nov 2023More than 200 genetic variants have been independently associated with prostate cancer risk. Studies among farmers have also observed increased prostate cancer risk...
More than 200 genetic variants have been independently associated with prostate cancer risk. Studies among farmers have also observed increased prostate cancer risk associated with exposure to specific organophosphate (fonofos, terbufos, malathion, dimethoate) and organochlorine (aldrin, chlordane) insecticides. We examined the joint associations between these pesticides, established prostate cancer loci, and prostate cancer risk among 1,162 cases (588 aggressive) and 2,206 frequency-matched controls nested in the Agricultural Health Study cohort. History of lifetime pesticide use was combined with a polygenic risk score (PRS) generated using 256 established prostate cancer risk variants. Logistic regression models estimated the joint associations of the pesticides, the PRS, and the 256 individual genetic variants with risk of total and aggressive prostate cancer. Likelihood ratio tests assessed multiplicative interaction. We observed interaction between ever use of fonofos and the PRS in relation to total and aggressive prostate cancer risk. Compared to the reference group (never use, PRS < median), men with ever use of fonofos and PRS > median had elevated risks of total (OR 1.35 [1.06-1.73], p-interaction = 0.03) and aggressive (OR 1.49 [1.09-2.04], p-interaction = 0.19) prostate cancer. There was also suggestion of interaction between pesticides and individual genetic variants occurring in regions associated with DNA damage response (CDH3, EMSY genes) and with variants related to altered androgen receptor-driven transcriptional programs critical for prostate cancer. Our study provides evidence that men with greater genetic susceptibility to prostate cancer may be at higher risk if they are also exposed to pesticides and suggests potential mechanisms by which pesticides may increase prostate cancer risk.
PubMed: 37659638
DOI: 10.1016/j.envres.2023.117063 -
Environmental Toxicology and Chemistry Dec 2023Currently, only Apis mellifera is used in environmental regulation to evaluate the hazard of pesticides to pollinators. The low representativeness of pollinators and bee...
Currently, only Apis mellifera is used in environmental regulation to evaluate the hazard of pesticides to pollinators. The low representativeness of pollinators and bee diversity in this approach may result in insufficient protection for the wild species. This scenario is intensified in tropical environments, where little is known about the effects of pesticides on solitary bees. We aimed to calculate the medium lethal dose (LD50) and medium lethal concentration (LC50) of the insecticide dimethoate in the Neotropical solitary bee Centris analis, a cavity-nesting, oil-collecting bee distributed from Brazil to Mexico. Males and females of C. analis were exposed orally to dimethoate for 48 h under laboratory conditions. Lethality was assessed every 24 h until 144 h after the beginning of the test. After the LD50 calculation, we compared the value with available LD50 values in the literature of other bee species using the species sensitivity distribution curve. In 48 h of exposure, males showed an LD50 value 1.33 times lower than females (32.78 and 43.84 ng active ingredient/bee, respectively). Centris analis was more sensitive to dimethoate than the model species A. mellifera and the solitary bee from temperate zones, Osmia lignaria. However, on a body weight basis, C. analis and A. mellifera had similar LD50 values. Ours is the first study that calculated an LD50 for a Neotropical solitary bee. Besides, the results are of crucial importance for a better understanding of the effects of pesticides on the tropical bee fauna and will help to improve the risk assessment of pesticides to bees under tropical conditions, giving attention to wild species, which are commonly neglected. Environ Toxicol Chem 2023;42:2758-2767. © 2023 SETAC.
Topics: Female; Bees; Animals; Insecticides; Pesticides; Dimethoate; Hymenoptera; Risk Assessment
PubMed: 37638658
DOI: 10.1002/etc.5738 -
Ecotoxicology and Environmental Safety Oct 2023Pesticide exposure is an important driver of bee declines. Laboratory toxicity tests provide baseline information on the potential effects of pesticides on bees, but...
Pesticide exposure is an important driver of bee declines. Laboratory toxicity tests provide baseline information on the potential effects of pesticides on bees, but current risk assessment schemes rely on one species, the highly social honey bee, Apis mellifera, and there is uncertainty regarding the extent to which this species is a suitable surrogate for other pollinators. For this reason, Osmia cornuta and Osmia bicornis have been proposed as model solitary bee species in the EU risk assessment scheme. The use of solitary bees in risk assessment requires the development of new methodologies adjusted to the biology of these species. For example, oral dosing methods used with honey bees cannot be readily applied to solitary bees due to differences in feeding behaviour and social interactions. In this study, we describe the "petal method", a laboratory feeding method, and validate its use in acute and chronic exposure oral tests with Osmia spp. We conducted five experiments in which we compared the performance of several artificial flowers combining visual and olfactory cues against the petal method, or in which variations of the petal method were confronted. We then use the results of these experiments to optimize the feeding arenas and propose standardized methods for both acute and chronic exposure tests. The petal method provides high levels of feeding success, thus reducing the number of bees needed. It works with a wide variety of petal species and with both female and male Osmia spp., thus ensuring reproducibility across studies. To validate the use of the petal method in ecotoxicology tests, we assess the toxicity of a standard reference insecticide, dimethoate, in O. cornuta adults and determine LD50 values for this species. The petal method should facilitate the inclusion of solitary bees in risk assessment schemes therefore increasing the protection coverage of pesticide regulation.
Topics: Male; Bees; Female; Animals; Pesticides; Reproducibility of Results; Insecticides; Flowers; Toxicity Tests
PubMed: 37634482
DOI: 10.1016/j.ecoenv.2023.115398