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The Lancet. Infectious Diseases Jul 2024
Topics: Humans; Peritonitis; Listeriosis; Listeria monocytogenes; Anti-Bacterial Agents; Male; Female; Middle Aged
PubMed: 38608699
DOI: 10.1016/S1473-3099(24)00182-8 -
The Lancet. Infectious Diseases Jul 2024Listeriosis is a foodborne infection caused by Listeria monocytogenes. Three main forms of listeriosis are well characterised, but little is known about L... (Observational Study)
Observational Study
BACKGROUND
Listeriosis is a foodborne infection caused by Listeria monocytogenes. Three main forms of listeriosis are well characterised, but little is known about L monocytogenes-associated spontaneous bacterial peritonitis. We used data from the French national surveillance of listeriosis to perform a nationwide retrospective study.
METHODS
All patients with L monocytogenes isolated by culture from a peritoneal fluid sample in France between April 1, 1993, and Dec 31, 2022, were included. Individuals for whom bacterial peritonitis was not confirmed and those who also had another type of invasive listeriosis were excluded. A standardised checklist was used to collect demographic, clinical, and biological data as well as antibiotic treatment and follow-up data. The primary outcome was to determine the characteristics of L monocytogenes-associated spontaneous bacterial peritonitis. We did descriptive analyses and assessed risk factors for 1-month mortality using an exploratory multivariable Cox model analysis.
FINDINGS
Among the 8768 L monocytogenes cases reported, 208 (2%) were patients with L monocytogenes-associated spontaneous bacterial peritonitis. Mean age was 65 years (SD 13), 50 (24%) of 208 patients were female, and 158 (76%) were male (no data on race or ethnicity were available). 200 (98%) of 205 patients with L monocytogenes-associated spontaneous bacterial peritonitis with available data had immunosuppressive comorbidities, including cirrhosis (148 [74%] of 201 with available data), ongoing alcoholism (58 [62%] of 94), and ongoing neoplasia (60 [31%] of 195). Causes of ascites included cirrhosis (146 [70%] of 208), ongoing neoplasia (26 [13%]), end-stage heart failure (13 [6%]), and peritoneal dialysis (11 [5%]). Among those with available data, presentation was pauci-symptomatic and non-specific; only 67 (50%) of 135 patients presented with fever, 49 (37%) of 132 with abdominal pain, and 27 (21%) of 129 with diarrhoea. 61 (29%) of 208 patients were dead at 1 month, 92 (44%) were dead at 3 months, and 109 (52%) were dead at 6 months after diagnosis. Ongoing neoplasia (hazard ratio 2·42 [95% CI 1·05-5·56]; p=0·039), septic shock (8·03 [2·66-24·02]; p=0·0021), and high blood leukocyte count (1·05 [1·00-1·09]; p=0·045) were independently associated with 1-month mortality.
INTERPRETATION
Despite the non-specific and mild presentation of L monocytogenes-associated spontaneous bacterial peritonitis, the outcome is poor and similar to that of neurolisteriosis, and so identification of L monocytogenes in ascitic fluid samples requires urgent parenteral amoxicillin-based treatment to avoid a fatal outcome.
FUNDING
Institut Pasteur, Inserm, and French Public Health Agency.
TRANSLATION
For the French translation of the abstract see Supplementary Materials section.
Topics: Humans; Male; Female; Peritonitis; Listeriosis; France; Aged; Listeria monocytogenes; Middle Aged; Retrospective Studies; Risk Factors; Aged, 80 and over; Anti-Bacterial Agents; Adult
PubMed: 38608698
DOI: 10.1016/S1473-3099(24)00151-8 -
Foodborne Pathogens and Disease Apr 2024Listeriosis is a globally rare foodborne disease that causes fetal-placental infection, leading to adverse pregnancy outcome, while limited research among pregnant women...
Listeriosis is a globally rare foodborne disease that causes fetal-placental infection, leading to adverse pregnancy outcome, while limited research among pregnant women is available in China. This study was therefore aimed at analyzing the incidence, clinical manifestations, and clinical outcome of listeriosis among pregnant women and its associated dietary behavior risk factors in prevention. A hospital-based case-control study had been conducted from January 2017 to December 2021. Clinical data, laboratory information, and questionnaires including dietary behaviors and personal hygiene were collected within 2 days after case diagnosis. There were 48 pregnant women, including 12 cases and 36 controls, with an average age of 31.19 ± 3.75 years. The incidence of admission-based listeriosis among pregnant women was 1.6058 per 10,000. The 12 strains were divided into 3 serotypes: 1/2a(83.33%), 1/2b(8.33%), and 4b(8.33%). Among the cases, 5 cases (41.67%) resulted in abortion, 3 cases (25%) induced preterm labor, and 4 cases (33.33%) had full-term deliveries after treatment. There were 7 live births in the case group, among which 6 were admitted to the neonatal intensive care unit (NICU), while 1 case had a healthy fetal outcome. All patients in the control group gave birth to live fetuses. Epidemiological investigation revealed that pregnant women dining at restaurants three or more times per week might increase the risk of having infection. There were no significant differences in dietary consumed behaviors, hand hygiene, and refrigerator usage behaviors between case and control groups. The study suggested that dining at restaurants might be associated with infection among pregnant women. Therefore, it is essential to enhance education on listeriosis serious consequences and promote healthy dietary and hygiene habits among pregnant women.
PubMed: 38607615
DOI: 10.1089/fpd.2023.0114 -
Veterinary Medicine (Auckland, N.Z.) 2024Listeriosis is an important foodborne zoonotic disease affecting humans and animals in Ethiopia. This review aims to synthesize the epidemiology, prevalence,... (Review)
Review
Listeriosis is an important foodborne zoonotic disease affecting humans and animals in Ethiopia. This review aims to synthesize the epidemiology, prevalence, distribution, and antimicrobial resistance of Listeria species in the country. The literature reveals a widespread occurrence of Listeria infection in humans, animals, and food products, with an average prevalence of 21.6% for Listeria species and 6.9% for . Three sequence types (STs) of (2, 145, and 18) and twelve STs of (1489, 1619, 603, 537, 1010, 3186, 492, 3007, 1087, 474, 1008, and 637) were reported from milk and dairy products. Contamination rates ranged from 4.1% to 42.9% across livestock, dairy, slaughterhouses, and processing facilities, indicating faults in production practices. Sporadic human listeriosis outbreaks have occurred since 1967, causing meningitis, perinatal infections, and deaths, with recent studies showing isolation in up to 10.4% of febrile patients, confirming foodborne transmission. Non-pathogenic Listeria species were also common on farms and in facilities. Ovine listeriosis poses a threat to Ethiopia's sheep and goat industries, with over 40% seroprevalence in some herds. Comprehensive control measures across the food chain are needed to curb contamination and protect public health. Isolates from various foods show antibiotic resistance to first-line agents but susceptibility to others like gentamicin and cephalosporins. In conclusion, this review synthesizes evidence on Listeria distribution in Ethiopia's food system and disease burden, highlighting the need for improved food safety policies and awareness.
PubMed: 38601062
DOI: 10.2147/VMRR.S451837 -
Journal of Immunology (Baltimore, Md. :... Jun 2024Ag-specific effector CD4+ T cells play a crucial role in defending against exogenous pathogens. However, the mechanisms governing the differentiation and function of...
Ag-specific effector CD4+ T cells play a crucial role in defending against exogenous pathogens. However, the mechanisms governing the differentiation and function of IFN-γ-producing effector CD4+ Th1 cells in immune responses remain largely unknown. In this study, we elucidated the pivotal role of zinc finger protein 335 (Zfp335) in regulating effector Th1 cell differentiation and survival during acute bacterial infection. Mice with Zfp335 knockout in OT-II cells exhibited impaired Ag-specific CD4+ T cell expansion accompanied by a significant reduction in resistance to Listeria infection. Furthermore, Zfp335 deficiency restricted the effector CD4+ Th1 cell population and compromised their survival upon Listeria challenge. The expression of T-bet and IFN-γ was accordingly decreased in Zfp335-deficient Th1 cells. Mechanistically, Zfp335 directly bound to the promoter region of the Lmna gene and regulated its expression. Overexpression of Lmna was able to rescue the survival and function of Zfp335-deficient effector Th1 cells. Therefore, our study provides novel insights into the mechanisms governing effector Th1 cell differentiation and survival during acute infection.
Topics: Animals; Mice; Cell Differentiation; Cell Survival; DNA-Binding Proteins; Gene Expression Regulation; Interferon-gamma; Lamin Type A; Listeriosis; Mice, Inbred C57BL; Mice, Knockout; Th1 Cells; Transcription Factors
PubMed: 38598411
DOI: 10.4049/jimmunol.2300833 -
Heliyon Apr 2024Listeriosis is a disease caused by , a relevant microorganism as a causative agent of foodborne diseases - FBD. This study aimed to evaluate the distribution of spp.,...
Listeriosis is a disease caused by , a relevant microorganism as a causative agent of foodborne diseases - FBD. This study aimed to evaluate the distribution of spp., and in different production areas in two small plants (A and B) and two micro-food processing plants (C and D) producing meat derivatives, located in different cities of Colombia. The methodology implemented was . The analysis of sampling points is based on a harmonised tool. . Four samplings in each production plant between 2019 and 2020. . Isolation and identification of microorganisms through conventional microbiology, a semi-automated system, molecular serotyping and clonal characterisation by ERIC-PCR. frequency in the production plants belonging to the study ranged between 5.9 and 28.6 %; for spp., plants A and D had isolated, plant A had the highest proportion, while for geno-serotypes found were: 1/2a, 1/2c, 4a-4c, 4b, 4d - 4e, with geno-serotype 4b as the most frequent. Furthermore, possible persistent isolates were detected in plant C as the feasible sources of contamination, based on failures in flow management, raw material contaminated with lack of standardised cooking processes and transfer of the microorganism through equipment and surfaces. Finally, in three of the four production plants assayed, or spp. were present in the packaging area in some of the samples taken during the study, which calls for increased and frequent monitoring, as well as constant technical support for the control of in micro and small-scale production plants.
PubMed: 38596116
DOI: 10.1016/j.heliyon.2024.e28662 -
Revista Espanola de Quimioterapia :... Jun 2024
Topics: Aged; Humans; Male; Anti-Bacterial Agents; Endocarditis, Bacterial; Listeria monocytogenes; Listeriosis
PubMed: 38594903
DOI: 10.37201/req/141.2023 -
BMJ Case Reports Apr 2024Listeriosis is a rare foodborne infection caused by It has been reported to be commonly found among the obstetric population, immunocompromised group and elderly,...
Listeriosis is a rare foodborne infection caused by It has been reported to be commonly found among the obstetric population, immunocompromised group and elderly, presumably due to the lower immunity status in these populations. Presentation in pregnancy is usually non-specific like fever, diarrhoea, respiratory tract symptoms and preterm rupture of membrane. These make the diagnosis challenging and may delay the correct management. We present a case of a female in her early 40s, gravida 4 para 0+3 at 27 weeks who presented with fever. She later developed preterm rupture of membrane 24 hours after admission. The leaking of liquor later changed from clear to meconium stained raising the suspicion of listeria chorioamnionitis, necessitating an emergency preterm delivery via caesarean section. The newborn acquired listeria infection and required ventilation support. He subsequently was discharged from neonatal unit after nearly 3 months of life.
Topics: Adult; Female; Humans; Infant, Newborn; Pregnancy; Cesarean Section; Chorioamnionitis; Fever; Listeria monocytogenes; Listeriosis; Premature Birth; Male
PubMed: 38589245
DOI: 10.1136/bcr-2024-259938 -
Microorganisms Feb 2024() is a pathogen that is transmitted through contaminated food and causes the illness known as listeriosis. The virulence factor InlA plays a crucial role in the...
() is a pathogen that is transmitted through contaminated food and causes the illness known as listeriosis. The virulence factor InlA plays a crucial role in the invasion of into the human intestinal epithelium. In addition, InlA enhances the pathogenicity of host strains, and different strains of contain varying variations of InlA. Our study analyzed a total of 4393 published genomes from 511 sequence types (STs) of diverse origins. We identified 300 unique InlA protein sequence types (PSTs) and revealed 45 highly mutated amino acid sites. The leucine-rich repeat (LRR) region was found to be the most conserved among the InlA, while the protein A (PA) region experienced the highest mutation rate. Two new types of mutations were identified in the B-repeat region of InlA. Correspondence analysis (CA) was used to analyze correlations between the lineages or 10 most common sequence types (STs) and amino acid (aa) sites. ST8 was strongly correlated with site 192_F, 454_T. ST7 exhibited a strong correlation with site 51_A, 573_E, 648_S, and 664_A, and it was also associated with ST6 and site 544_N, 671_A, 738_B, 739_B, 740_B, and 774_Y. Additionally, a strong correlation between ST1 and site 142_S, 738_N, ST2 and site 2_K, 142_S, 738_N, as well as ST87 and site2_K, 738_N was demonstrated. Our findings contribute significantly to the understanding of the distribution, composition, and conservation of InlA in These findings also suggest a potential role of InlA in supporting molecular epidemiological tracing efforts.
PubMed: 38543536
DOI: 10.3390/microorganisms12030485 -
International Journal of Molecular... Mar 2024() is a food-borne pathogenic bacteria that frequently contaminates animal-derived food and low-temperature preserved food. caused by its infection has a high...
() is a food-borne pathogenic bacteria that frequently contaminates animal-derived food and low-temperature preserved food. caused by its infection has a high mortality rate and poses a serious threat to human health. Therefore, it is crucial to establish a sensitive, rapid and easy-to-operate technique. In this study, a Recombinase Aided Amplification (RAA) assisted CRISPR/Cas12a (RAA-CRISPR/Cas12a) fluorescence platform was established for highly sensitive nucleic acid detection of . The established RAA-CRISPR/Cas12a showed high sensitivity and high specificity, with the sensitivity of 350 CFU/mL and 5.4 × 10 ng/μL for pure bacterial solution and genomic DNA, and good specificity for 5 strains of spp. and 14 strains of other common pathogenic bacteria. could be detected at an initial concentration of 2.3 CFU/25g within 2 h of enriching the beef in the food matrix, and this method could be applied to food samples that were easily contaminated with The results of RAA-CRISPR/Cas12a could be observed in 5 min, while the amplification was completed in 20-30 min. The speed and sensitivity of RAA-CRISPR/Cas12a were significantly higher than that of the national standard method. In conclusion, the RAA-CRISPR/Cas12a system established in this study has new application potential in the diagnosis of food-borne pathogens.
Topics: Animals; Cattle; Humans; Listeria monocytogenes; CRISPR-Cas Systems; Food Microbiology; Nucleic Acid Amplification Techniques; Recombinases; DNA
PubMed: 38542449
DOI: 10.3390/ijms25063477