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Current Microbiology Jul 2023The polymicrobial nature of diabetic foot infection (DFI) makes accurate identification of the DFI microbiota, including rapid detection of drug resistance, challenging....
The polymicrobial nature of diabetic foot infection (DFI) makes accurate identification of the DFI microbiota, including rapid detection of drug resistance, challenging. Therefore, the main objective of this study was to apply matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) technique accompanied by multiply culture conditions to determine the microbial patterns of DFIs, as well as to assess the occurrence of drug resistance among Gram-negative bacterial isolates considered a significant cause of the multidrug resistance spread. Furthermore, the results were compared with those obtained using molecular techniques (16S rDNA sequencing, multiplex PCR targeting drug resistance genes) and conventional antibiotic resistance detection methods (Etest strips). The applied MALDI-based method revealed that, by far, most of the infections were polymicrobial (97%) and involved many Gram-positive and -negative bacterial species-19 genera and 16 families in total, mostly Enterobacteriaceae (24.3%), Staphylococcaceae (20.7%), and Enterococcaceae (19.8%). MALDI drug-resistance assay was characterized by higher rate of extended-spectrum beta-lactamases (ESBLs) and carbapenemases producers compared to the reference methods (respectively 31% and 10% compared to 21% and 2%) and revealed that both the incidence of drug resistance and the species composition of DFI were dependent on the antibiotic therapy used. MALDI approach included antibiotic resistance assay and multiply culture conditions provides microbial identification at the level of DNA sequencing, allow isolation of both common (eg. Enterococcus faecalis) and rare (such as Myroides odoratimimus) bacterial species, and is effective in detecting antibiotic-resistance, especially those of particular interest-ESBLs and carbapenemases.
Topics: Humans; Diabetic Foot; Enterobacteriaceae; Anti-Bacterial Agents; Enterobacteriaceae Infections; Gram-Negative Bacteria; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Diabetes Mellitus
PubMed: 37405539
DOI: 10.1007/s00284-023-03384-z -
Japanese Journal of Infectious Diseases Nov 2023Myroides species have recently been reported more frequently in outbreaks in clinics and intensive care units (ICUs). In this study, we aimed to investigate the epidemic...
Myroides species have recently been reported more frequently in outbreaks in clinics and intensive care units (ICUs). In this study, we aimed to investigate the epidemic potential, antibiotic resistance profile, and risk factors of M. odoratimimus isolates that are increasingly being isolated from the ICUs of our hospital. Data from patients whose Myroides spp. were isolated from their clinical specimens over a 5-year period (September 2016 to January 2022) were retrospectively analyzed. Bacterial identification was performed using a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The presence of antibiotic resistance genes was analyzed using polymerase chain reaction (PCR). Possible clonal associations between isolates were investigated using enterobacterial repetitive intergenic consensus (ERIC)-PCR. As a result, 66 isolates were identified as M. odoratimimus and one isolate was identified as M. odoratus. The bla resistance gene was detected in all M. odoratimimus isolates, whereas sul2 was detected in ten isolates and tetX was detected in 11 isolates. No other resistance genes, such as bla, were detected. Additionally, two different clonal association patterns were discovered in the 24 selected isolates through the ERIC-PCR method. The increase in the immunosuppressive patient population indicate the possibility of encountering this agent and other opportunistic pathogens more frequently in the future.
Topics: Humans; Persistent Infection; Retrospective Studies; Enterobacteriaceae; Drug Resistance, Multiple, Bacterial; Anti-Bacterial Agents; Disease Outbreaks; Hospitals; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 37394461
DOI: 10.7883/yoken.JJID.2023.005 -
Waste Management & Research : the... Sep 2023Black soldier fly larvae (BSFL) hold great promise for sustainable management of meat and bone meal (MBM), a kind of organic waste. Harvested BSFL frass can be used as...
Black soldier fly larvae (BSFL) hold great promise for sustainable management of meat and bone meal (MBM), a kind of organic waste. Harvested BSFL frass can be used as soil amendment or organic fertilizer. This study evaluated the quality and microbial profile in the frass of BSFL, fed with fish MBM containing 0% (CK), 1% (T1), 2% (T2) and 3% (T3) of rice straw. Results suggested straw addition into fish MBM had no significant impacts on BSFL weight; however, straw addition remarkably affected waste reduction and conversion efficiency, as well as physicochemical properties including electric conductivity, organic matter (OM) and total phosphorus contents in frass. Fourier transform infrared analysis indicated that increasing levels of cellulose and lignin might not be fully degraded or transformed by BSFL when more straw was introduced into substrates. Straw addition had hardly significant influences on microbial richness or evenness in BSFL frass, only T3 treatment remarkably elevated the phylogenetic diversity value more than the control. , , and were the most dominant phyla. Genera , and maintained high abundances in all frass samples. Elements including OM, pH and Na were key factors in shaping the microbiological characteristics of BSFL frass. Our findings helped to understand the effects of fish MBM waste manipulation on BSFL frass qualities and contributed to the further application of BSFL frass.
Topics: Animals; Larva; Phylogeny; Diptera; Meat
PubMed: 36951008
DOI: 10.1177/0734242X231160091