-
Antonie Van Leeuwenhoek Apr 2024The deoxynivalenol (DON)-degrading bacterium JB1-3-2 was isolated from a rhizosphere soil sample of cucumber collected from a greenhouse located in Zhenjiang, Eastern...
The deoxynivalenol (DON)-degrading bacterium JB1-3-2 was isolated from a rhizosphere soil sample of cucumber collected from a greenhouse located in Zhenjiang, Eastern China. The JB1-3-2 strain is a Gram-stain-positive, nonmotile and round actinomycete. Growth was observed at temperatures between 15 and 40 ℃ (optimum, 35 ℃), in the presence of 15% (w/v) NaCl (optimum, 3%), and at pH 3 and 11 (optimum, 7). The major cellular fatty acids identified were anteiso-C, iso-C and anteiso-C. Genome sequencing revealed a genome size of 4.11 Mb and a DNA G + C content of 72.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the JB1-3-2 strain was most closely related to type strains of the Oerskovia species, with the highest sequence similarity to Oerskovia turbata NRRL B-8019 (98.2%), and shared 98.1% sequence identity with other valid type strains of this genus. Digital DNA‒DNA hybridization (dDDH) and average nucleotide identity (ANI) showed 21.8-22.2% and 77.2-77.3% relatedness, respectively, between JB1-3-2 and type strains of the genus Oerskovia. Based on genotypic, phylogenetic, chemotaxonomic, physiological and biochemical characterization, Oerskovia flava, a novel species in the genus Oerskovia, was proposed, and the type strain was JB1-3-2 (= CGMCC 1.18555 = JCM 35248). Additionally, this novel strain has a DON degradation ability that other species in the genus Oerskovia do not possess, and glutathione-S-transferase was speculated to be the key enzyme for strain JB1-3-2 to degrade DON.
Topics: Soil Microbiology; Rhizosphere; Phylogeny; Cucumis sativus; Trichothecenes; RNA, Ribosomal, 16S; Fatty Acids; DNA, Bacterial; China; Base Composition; Bacterial Typing Techniques; Sequence Analysis, DNA; Genome, Bacterial
PubMed: 38676821
DOI: 10.1007/s10482-024-01972-y