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International Journal of Biological... Jun 2024Lignocellulose is an abundant renewable bio-macromolecular complex, which can be used to produce biomethane and other high-value products. The lignin, presents in...
A coupling strategy combined with acid-hydrothermal and novel DES pretreatment: Enhancing biomethane yield under solid-state anaerobic digestion and efficiently producing xylo-oligosaccharides and recovered lignin from poplar waste.
Lignocellulose is an abundant renewable bio-macromolecular complex, which can be used to produce biomethane and other high-value products. The lignin, presents in lignocellulose is typically regarded as an inhibitor of anaerobic digestion. Therefore, it is crucial to develop a novel selective separation strategy to achieve efficient biomethane production and all-component utilization of biomass. Hence, a combination of two-step pretreatment and solid-state anaerobic digestion was employed to enhance the production of biomethane and to generate valuable chemicals from poplar waste. Optimal conditions (4 % acetic acid, 170 °C, and 40 min) resulted in 70.85 % xylan removal, yielding 50.28 % xylo-oligosaccharides. The effect of a strong acid 4-CSA-based novel three-constituent DES on delignification was investigated from 80 °C to 100 °C; the cellulose content of DES pretreated poplar increased from 64.11 % to 80.92 %, and the delignification rate increased from 49.0 % to 90.4 %. However, high delignification of the pretreated poplar (DES-100 and DES-110) led to a rapid accumulation of volatile organic acids during the hydrolysis and acidogenesis stages, resulting in methanogenesis inhibition. The highest biomethane yield of 208 L/kg VS was achieved with DES-80 (49.0 % delignification), representing a 148 % improvement compared over untreated poplar. This approach demonstrates the potential for comprehensive utilization of all components of biomass waste.
PubMed: 38942405
DOI: 10.1016/j.ijbiomac.2024.133443 -
Environment International Jun 2024As the COVID-19 pandemic has progressed, increasing evidences suggest that the gut microbiota may play a crucial role in the effectiveness of SARS-CoV-2 vaccine. Thus,...
As the COVID-19 pandemic has progressed, increasing evidences suggest that the gut microbiota may play a crucial role in the effectiveness of SARS-CoV-2 vaccine. Thus, this study was aimed at investigating the influence of SARS-CoV-2 vaccine on the gut microbiota and short-chain fatty acids (SCFAs) of organisms exposed to environmental contaminants, i.e., plasticizers: phthalate esters. We found that in mice, exposure to dioctyl terephthalate (DOTP) and bis -2-ethylhexyl phthalate (DEHP) decreased the blood glucose level and white fat weight, induced inflammatory responses, caused damage to liver and intestinal tissues, and disrupted the gut microbiota composition and SCFAs metabolism. Specifically, the Bacteroidetes phylum was positively correlated with BBIBP-CorV vaccine, while acetic acid was negatively associated with the vaccine. Interestingly, the BBIBP-CorV vaccine somewhat alleviated tissue inflammation and reduced the contents of acetic acid and propionic acid in mice exposed to DEHP and DOTP. These findings were confirmed by a fecal microbiota transplantation assay. Overall, this study revealed that exposure to DEHP and DOTP adversely affects the gut microbiota and SCFAs, while the BBIBP-CorV vaccine can protect mice against these effects. This work highlighted the relationship between BBIBP-CorV vaccination, gut microbiome composition, and responses to plasticizers, which may facilitate the development and risk assessment of SARS-CoV-2 vaccines and environmental contaminants on microbiota health.
PubMed: 38941942
DOI: 10.1016/j.envint.2024.108851 -
Journal of Colloid and Interface Science Jun 2024Dielectric effects and the coupled electrostatics between the nanoconfined and the internal/external aqueous media contribute to the observed deviations of chemistry...
Dielectric effects and the coupled electrostatics between the nanoconfined and the internal/external aqueous media contribute to the observed deviations of chemistry within the nanoconfined environment when compared with unconfined systems. A systematic understanding has remained elusive, especially with respect to background salt concentration and boundary condition effects like the nanopore surface chemistry and the reference state used to calculate free energies. We utilize molecular dynamics simulations along with thermodynamic integration to determine the free energy difference associated with acid-base chemistry in 2 nm and 4 nm slit pores open to a bulk-like reservoir. pK increases are predicted when confining acetic acid, formic acid, and bicarbonate in the slits at infinite dilution conditions. We find that confinement weakens the acids, and the modulation of outer pore surface dipole magnitudes can tune the pK shift values, suggesting that purely "intrinsic" electrostatic effect on confinement may not exist. At sufficiently high salt concentrations, the dielectric/electrostatic effects on pK values diminish due to charge screening effects. These discoveries enable future modifications of nanopore chemistries to achieve desirable properties for industrial applications.
PubMed: 38941940
DOI: 10.1016/j.jcis.2024.05.160 -
International Journal of Biological... Jun 2024This work was to investigate the effect of four prebiotic saccharides gum arabic (GA), fructooligosaccharide (FOS), konjac glucomannan (KGM), and inulin (INU)...
This work was to investigate the effect of four prebiotic saccharides gum arabic (GA), fructooligosaccharide (FOS), konjac glucomannan (KGM), and inulin (INU) incorporation on the encapsulation efficiency (EE), physicochemical stability, and in vitro digestion of urolithin A-loaded liposomes (UroA-LPs). The regulation of liposomes on gut microbiota was also investigated by in vitro colonic fermentation. Results indicated that liposomes coated with GA showed the best EE, bioaccessibility, storage and thermal stability, the bioaccessibility was 1.67 times of that of UroA-LPs. The UroA-LPs coated with FOS showed the best freeze-thaw stability and transformation. Meanwhile, saccharides addition remarkably improved the relative abundance of Bacteroidota, reduced the abundances of Proteobacteria and Actinobacteria. The UroA-LPs coated with FOS, INU, and GA exhibited the highest beneficial bacteria abundance of Parabacteroides, Monoglobus, and Phascolarctobacterium, respectively. FOS could also decrease the abundance of harmful bacteria Collinsella and Enterococcus, and increase the levels of acetic acid, butyric acid and iso-butyric acid. Consequently, prebiotic saccharides can improve the EE, physicochemical stability, gut microbiota regulation of UroA-LPs, and promote the bioaccessibility of UroA, but the efficiency varied based on saccharides types, which can lay a foundation for the application of UroA in foods industry and for the enhancement of its bio-activities.
PubMed: 38942666
DOI: 10.1016/j.ijbiomac.2024.133045 -
Methods in Molecular Biology (Clifton,... 2024The proteomic approach plays a key role to characterize a biological system at any given time. In recent years, advances in proteomics have led to an increasing...
The proteomic approach plays a key role to characterize a biological system at any given time. In recent years, advances in proteomics have led to an increasing application in all biological fields, including plant matrices and associated microbiome studies. However, extracting adequate protein samples remains the most critical step for any plant proteomics study. The protein extraction protocols proposed for the phyllosphere involve an initial leaf washing step; however, this is an approach only applicable if interest is restricted to epiphytes. A metaproteomic approach is required to obtain an overall picture and consequently an extraction that considers proteins derived from the plant, epiphytic and endophytic microorganisms. The most commonly used extractions for plant tissue involve the use of phenol or TCA-acetone. However, for efficient protein recovery is essential to remove interfering components abundant in plant tissues, such as polysaccharides, lipids, and phenolic compounds. A well-proven protocol on the basis of a combination of TCA-acetone and phenol extraction is presented here, obtaining some cleaned protein pellets, suitable for electrophoresis and subsequent proteomics studies. Important points for the success of this protocol are (i) a proper sampling and sample preparation, (ii) maintaining samples at a low temperature during extraction and using protease inhibitors, (iii) an initial step in TCA-acetone to remove part of the interfering substances, and (iv) careful recovery of the phenolic phase. Furthermore, the protocol is timesaving and can be completed in one working day.
Topics: Plant Proteins; Proteomics; Plant Leaves; Acetone; Phenol; Plants; Trichloroacetic Acid
PubMed: 38941013
DOI: 10.1007/978-1-0716-3910-8_5 -
Food & Function Jun 2024Copper II oxide nanoparticles (CuO NPs), a kind of widely used nanomaterial, have been detected in food and the environment, which has aroused widespread public concern....
Copper II oxide nanoparticles (CuO NPs), a kind of widely used nanomaterial, have been detected in food and the environment, which has aroused widespread public concern. Recently, increasing data have suggested that intestinal microecology is closely related to immune homeostasis. However, the intestinal immunotoxicity induced by CuO NPs through intestinal microbiota is still unknown. Therefore, in this study, zebrafish were exposed to CuO NPs to explore intestinal immunotoxicity by evaluating physiological indicators, intestinal tissue injury, antioxidant enzyme activities, gene expression of immune factors, and changes in intestinal microbiota and its metabolites (short-chain fatty acids (SCFAs) and lipopolysaccharides (LPS)). The results revealed that the intestinal immunotoxicity of CuO NPs was mediated by the impact on intestinal microbiota and its metabolite levels. Specifically, changes were observed in the abundance of microbes that participated in the metabolism of SCFAs and LPS. The reduction in acetic acid, propionic acid and valeric acid upregulated GPR84 expression, and the decline in LPS levels further resulted in the suppression of the key immune regulatory pathways TLR4/MyD88/NF-κB, ultimately leading to intestinal immunotoxicity. This study would provide a scientific basis for the risk assessment of CuO NPs and a new perspective for research on the immunotoxicity of nanoparticles.
PubMed: 38940701
DOI: 10.1039/d4fo01032a -
MSystems Jun 2024Skin ulceration syndrome (SUS) is currently the main disease threatening aquaculture due to its higher mortality rate and infectivity, which is caused by . Our previous...
Skin ulceration syndrome (SUS) is currently the main disease threatening aquaculture due to its higher mortality rate and infectivity, which is caused by . Our previous studies have demonstrated that SUS is accompanied by intestinal microbiota (IM) dysbiosis, alteration of short-chain fatty acids (SCFAs) content and the damage to the intestinal barrier. However, the mediating effect of IM on intestine dysfunction is largely unknown. Herein, we conducted comprehensive intestinal microbiota transplantation (IMT) to explore the link between IM and SUS development. Furthermore, we isolated and identified a strain with an ability to produce acetic acid from both healthy individual and SUS individual with IM from healthy donors. We found that dysbiotic IM and intestinal barrier function in SUS recipients could be restored by IM from healthy donors. The strain could restore IM community and intestinal barrier function. Consistently, acetate supply also restores intestinal homeostasis of SUS-diseased and -infected . Mechanically, acetate was found to specifically bind to its receptor-free fatty acid receptor 2 (FFAR2) to mediate IM structure community and intestinal barrier function. Knockdown of FFAR2 by transfection of specific FFAR2 siRNA could hamper acetate-mediated intestinal homeostasis . Furthermore, we confirmed that acetate/FFAR2 could inhibit -activated NF-κB-MLCK-MLC signaling pathway to restore intestinal epithelium integrity and upregulated the expression of ZO-1 and Occludin. Our findings provide the first evidence that restores pathogen-induced intestinal barrier dysfunction via acetate/FFAR2-NF-κB-MLCK-MLC axis, which provides new insights into the control and prevention of SUS outbreak from an ecological perspective.IMPORTANCESkin ulceration syndrome (SUS) as a main disease in aquaculture has severely restricted the developmental aquaculture industry. Intestinal microbiota (IM) has been studied extensively due to its immunomodulatory properties. Short-chain fatty acids (SCFAs) as an essential signal molecule for microbial regulation of host health also have attracted wide attention. Therefore, it is beneficial to explore the link between IM and SUS for prevention and control of SUS. In the study, the contribution of IM to SUS development has been examined. Additionally, our research further validated the restoration of SCFAs on intestinal barrier dysfunction caused by SUS via isolating SCFAs-producing bacteria. Notably, this restoration might be achieved by inhibition of NF-κB-MLCK-MLC signal pathway, which could be activated by . These findings may have important implications for exploration of the role of IM in SUS occurrence and provide insight into the SUS treatment.
PubMed: 38940521
DOI: 10.1128/msystems.00602-24 -
European Journal of Clinical... Jun 2024The rise in obesity highlights the need for improved therapeutic strategies, particularly in addressing metabolic dysfunction-associated steatotic liver disease (MASLD)....
BACKGROUND AND AIMS
The rise in obesity highlights the need for improved therapeutic strategies, particularly in addressing metabolic dysfunction-associated steatotic liver disease (MASLD). We aim to assess the role of tryptophan metabolic pathways in the pathogenesis of obesity and in the different histological stages of MASLD.
MATERIALS AND METHODS
We used ultra-high performance liquid chromatography to quantify circulating levels of 15 tryptophan-related metabolites from the kynurenine, indole and serotonin pathways. A cohort of 76 subjects was analysed, comprising 18 subjects with normal weight and 58 with morbid obesity, these last being subclassified into normal liver (NL), simple steatosis (SS) and metabolic dysfunction-associated steatohepatitis (MASH). Then, we conducted gene expression analysis of hepatic IDO-1 and kynyrenine-3-monooxygenase (KMO).
RESULTS
Key findings in obesity revealed a distinct metabolic signature characterized by a higher concentration of different kynurenine-related metabolites, a decrease in indole-3-acetic acid and indole-3-propionic acid, and an alteration in the serotonin pathway. Elevated tryptophan levels were associated with MASLD presence (37.659 (32.577-39.823) μM of tryptophan in NL subjects; 41.522 (38.803-45.276) μM in patients with MASLD). Overall, pathway fluxes demonstrated an induction of tryptophan catabolism via the serotonin pathway in SS subjects and into the kynurenine pathway in MASH. We found decreased IDO-1 and KMO hepatic expression in NL compared to SS.
CONCLUSIONS
We identified a distinctive metabolic signature in obesity marked by changes in tryptophan catabolic pathways, discernible through altered metabolite profiles. We observed stage-specific alterations in tryptophan catabolism fluxes in MASLD, highlighting the potential utility of targeting these pathways in therapeutic interventions.
PubMed: 38940215
DOI: 10.1111/eci.14279 -
Frontiers in Bioscience (Elite Edition) May 2024The utilization of various agrochemicals in crop production technology leads to soil health and fertility depletion. Multiple measures have been taken to revitalize the... (Review)
Review
The utilization of various agrochemicals in crop production technology leads to soil health and fertility depletion. Multiple measures have been taken to revitalize the health of polluted soil. In this context, organic agriculture has increased over the past few years to overcome the detrimental effects of extensive modern agricultural practices. Several traditional organic formulations, such as panchagavya, jeevamurtha, beejamurtha, bokashi, etc., are vital in converting polluted farmlands into organic. Various countries have their own organic formulations to improve crop growth and yield. These formulations are rich sources of many macro and micronutrients, growth-promoting phytohormones, and provide resistance against biotic and abiotic stresses. Apart from these benefits, these formulations consist of several groups of beneficial microorganisms that belong to the phyla , , , and , while some of the novel groups of microorganisms were also reported from the ingredients used in the preparation of these organic formulations. These microorganisms can solubilize nutrients such as phosphorous and zinc, oxidize sulfur, reduce nitrate, and are also involved in the production of indole acetic acid, ethylene reduction enzyme (1-aminocyclopropane-1-carboxylic acid deaminase), and organic acids that promote plant growth and induce resistance in the plant system. Hence, the utilization of traditional organic formulations helps in the reclamation of environmental health without compromising crop yields. This review describes the importance of organic farming, the preparation and application of different types of traditional organic formulations in different countries, and the microbial composition and mechanism of growth promotion of different traditional organic formulations.
Topics: Crops, Agricultural; Organic Agriculture; Soil Microbiology
PubMed: 38939912
DOI: 10.31083/j.fbe1602014 -
Frontiers in Public Health 2024Pesticides are widely used in agricultural activities. Although pesticide use is known to cause damage to the human body, its relationship with thyroid function remains...
BACKGROUND
Pesticides are widely used in agricultural activities. Although pesticide use is known to cause damage to the human body, its relationship with thyroid function remains unclear. Therefore, this study aimed to investigate the association between pesticide exposure and thyroid function.
METHODS
The Chinese database used included 60 patients with pyrethroid poisoning and 60 participants who underwent health checkups between June 2022 and June 2023. The NHANES database included 1,315 adults enrolled from 2007 to 2012. The assessed pesticide and their metabolites included 2,4-dichlorophenoxyacetic acid (2,4-D), 4-fluoro-3-phenoxybenzoic acid (4F3PB), para-nitrophenol (PN), 3-phenoxybenzoic acid (3P), and trans-dichlorovinyl-dimethylcyclopropane carboxylic acid (TDDC). The evaluated indicators of thyroid function were measured by the blood from the included population. The relationship between pesticide exposure and thyroid function indexes was investigated using linear regression, Bayesian kernel machine regression (BKMR), restricted cubic spline (RCS), and weighted quantile sum (WQS) models.
RESULTS
The Chinese data showed that pesticide exposure was negatively correlated with the thyroid function indicators FT4, TT4, TgAb, and TPOAb (all < 0.05). The BKMR model analysis of the NHANES data showed that the metabolic mixture of multiple pesticides was negatively associated with FT4, TSH, and Tg, similar to the Chinese database findings. Additionally, linear regression analysis demonstrated positive correlations between 2,4-D and FT3 ( = 0.041) and 4F3PB and FT4 ( = 0.003), whereas negative associations were observed between 4F3PB and Tg ( = 0.001), 4F3PB and TgAb ( = 0.006), 3P and TgAB ( = 0.006), 3P and TPOAb ( = 0.03), PN and TSH ( = 0.003), PN and TT4 ( = 0.031), and TDDC and TPOAb ( < 0.001). RCS curves highlighted that most pesticide metabolites were negatively correlated with thyroid function indicators. Finally, WQS model analysis revealed significant differences in the weights of different pesticide metabolites on the thyroid function indexes.
CONCLUSION
There is a significant negative correlation between pesticide metabolites and thyroid function indicators, and the influence weights of different pesticide metabolites on thyroid function indicators are significantly different. More research is needed to further validate the association between different pesticide metabolites and thyroid disease.
Topics: Humans; Pesticides; Male; Female; Middle Aged; China; Adult; Thyroid Gland; Nutrition Surveys; Thyroid Function Tests; Environmental Exposure; Databases, Factual; Aged; 2,4-Dichlorophenoxyacetic Acid; East Asian People
PubMed: 38939562
DOI: 10.3389/fpubh.2024.1378027