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Clinical Case Reports May 2021, a Gram-negative coccobacillus, is an emergent opportunistic pathogen. It originates from soil and water. VITEK and MALDI-TOF are used for identification. There are few...
, a Gram-negative coccobacillus, is an emergent opportunistic pathogen. It originates from soil and water. VITEK and MALDI-TOF are used for identification. There are few reports of peritoneal dialysis peritonitis. Its presentation is usually indolent. It can be successfully treated with several antibiotics: β-lactams, aminoglycosides, without removing the catheter.
PubMed: 34026181
DOI: 10.1002/ccr3.4176 -
Enzyme and Microbial Technology Feb 2021This work investigated the use of redox-active polymers based on bovine serum albumin and chitosan, covalently bound to mediators neutral red and ferrocene and...
This work investigated the use of redox-active polymers based on bovine serum albumin and chitosan, covalently bound to mediators neutral red and ferrocene and containing carbon nanotubes, for immobilization of Paracoccus yeei VKM B-3302 bacteria. The structures of produced polymers were studied by IR spectroscopy and scanning electron microscopy. Cyclic voltammetry and impedance spectroscopy found the electrochemical characteristics of the investigated systems: the heterogeneous electron transfer rate constant, the constant of the rate of interaction with P. yeei bacteria and the impedance. The systems containing carbon nanotubes and ferrocene-based redox-active polymer proved to be the most promising. Biosensors formed using the hybrid polymers had a high sensitivity with the lower boundary of 0.1 mg/dm of the detected BOD concentrations and a high correlation (R = 0.9916) with the standard BOD assay of surface water samples.
Topics: Biosensing Techniques; Electrochemical Techniques; Electrodes; Nanotubes, Carbon; Oxidation-Reduction; Paracoccus; Polymers
PubMed: 33375974
DOI: 10.1016/j.enzmictec.2020.109706 -
Microorganisms Dec 2020Mobile phones (MPs) of healthcare workers (HCWs) may represent an important source of transmission of infectious agents. This longitudinal study documents the...
Mobile phones (MPs) of healthcare workers (HCWs) may represent an important source of transmission of infectious agents. This longitudinal study documents the contamination of these tools. Ten MPs handled by senior pediatricians were sampled once a week during 23 weeks in three pediatric wards of the University Hospital of Saint-Etienne, France. Cultures were performed for bacteria and multiplex PCR assays for a panel of respiratory and enteric viruses. A questionnaire on hygiene habits regarding phoning and care was filled-in by pediatricians before and after the study. From a total of 230 samples, 145 (63%) were contaminated by at least one pathogen. The MPs from emergency departments were the most impacted. Viruses were detected in 179 samples; bacteria were isolated in 59 samples. Contamination increased during the winter epidemic peak. A cross-contamination by between hands and MPs of different HCWs was demonstrated. The communication of the study results influenced the hygiene behaviors. This study highlights the contamination of MPs by pathogens that are resistant in the environment, and its sustainability along the winter season. The role of MPs as vectors of nosocomial infection needs to be better investigated.
PubMed: 33339327
DOI: 10.3390/microorganisms8122011 -
Ocular Immunology and Inflammation May 2022
Topics: Angiogenesis Inhibitors; Anti-Bacterial Agents; Bacteria; Endophthalmitis; Eye Infections, Bacterial; Humans; Intravitreal Injections; Paracoccus; Retrospective Studies
PubMed: 33216649
DOI: 10.1080/09273948.2020.1836233 -
Therapeutic Apheresis and Dialysis :... Oct 2021
Topics: Anti-Bacterial Agents; Ceftriaxone; Gram-Negative Bacterial Infections; Humans; Male; Middle Aged; Paracoccus; Peritoneal Dialysis; Peritonitis
PubMed: 33135289
DOI: 10.1111/1744-9987.13604 -
Microorganisms Oct 2020Long considered to be a consequence of human antibiotics use by deduction, antibiotic resistance mechanisms appear to be in fact a much older phenomenon as antibiotic...
Long considered to be a consequence of human antibiotics use by deduction, antibiotic resistance mechanisms appear to be in fact a much older phenomenon as antibiotic resistance genes have previously been detected from millions of year-old permafrost samples. As these specimens guarantee the viability of archaic bacteria, we herein propose to apply the culturomics approach to recover the bacterial content of a Siberian permafrost sample dated, using the in situ-produced cosmogenic nuclide chlorine36 (Cl), at 2.7 million years to study the dynamics of bacterial evolution in an evolutionary perspective. As a result, we cultured and sequenced the genomes of 28 ancient bacterial species including one new species. To perform genome comparison between permafrost strains and modern isolates we selected 7 of these species (i.e., and ). We observed a high level of variability in genomic content with a percentage of shared genes in the core genomes ranging from 21.23% to 55.59%. In addition, the Single Nucleotide Polymorphism (SNP) comparison between permafrost and modern strains for the same species did not allow a dating of ancient strains based on genomic content. There were no significant differences in antibiotic resistance profiles between modern and ancient isolates of each species. Acquired resistance to antibiotics was phenotypically detected in all gram-negative bacterial species recovered from permafrost, with a significant number of genes coding for antibiotic resistance detected. Taken together, these findings confirm previously obtained data that antibiotic resistance predates humanity as most of antimicrobial agents are natural weapons used in inter-microbial conflicts within the biosphere.
PubMed: 33023015
DOI: 10.3390/microorganisms8101522 -
Journal of Biotechnology Aug 2020The CO fixation by autotrophic microbes has an important significance for improving carbon sequestration in composting. In this work, the succession of autotrophic...
The CO fixation by autotrophic microbes has an important significance for improving carbon sequestration in composting. In this work, the succession of autotrophic CO-fixing bacterial communities was investigated using quantitative PCR and high-throughput sequencing of the ribulose-1,5-bisphosphate carboxylase/oxygenase genes (cbbL and cbbM). The results presented that the number of autotrophic CO-fixing bacteria was comparable to that in the soil, and most species have not been classified into known bacterial groups, only part of them was assigned into Proteobacteria and Actinobacteria. Phylogenetic analysis displayed that the dominant known cbbL-containing bacteria were Allochromatium vinosum, Rhodobacter sphaeroides, Nitrobacter winogradskyi, Paracoccus yeei and Porphyrobacter sp. CACIAM 03H1, while the dominant known cbbM-containing bacteria were Sulfuritalea hydrogenivorans, Pseudomonas resinovorans, Achromobacter xylosoxidans, Sphingopyxis macrogoltabida and Thermomonospora curvata. In addition, canonical correspondence analysis showed that the evolution of autotrophic CO-fixing bacterial communities was greatly affected by physico-chemical parameters such as temperature, C/N and pH.
Topics: Autotrophic Processes; Bacteria; Bacterial Proteins; Carbon Dioxide; Composting; High-Throughput Nucleotide Sequencing; Microbial Consortia; Ribulose-Bisphosphate Carboxylase
PubMed: 32553532
DOI: 10.1016/j.jbiotec.2020.06.010 -
3 Biotech May 2020This work investigated the properties of VKM B-3302 bacteria isolated from activated sludge and immobilized in an N-vinylpyrrolidone-modified poly(vinyl alcohol)...
This work investigated the properties of VKM B-3302 bacteria isolated from activated sludge and immobilized in an N-vinylpyrrolidone-modified poly(vinyl alcohol) matrix. The developed hydrogel formed a network structure to enable the entrapment of microbial cells with their viability and biocatalytic properties preserved, which ensured the technological possibility of replicating expendable biosensor receptor elements. A new ratio of the components for the synthesis selected in this work enabled producing a copolymer of an earlier undescribed chemical structure, which can be efficiently used for immobilization of highly sensitive bacteria. A biological oxygen demand (BOD) biosensor with these bacteria and matrix was shown to possess a long-time stability exceeding that described earlier, to have a broad substrate specificity and to exceed approximately tenfold the nearest analogues by its sensitivity and the lower boundary value of 0.05 mg/dm. The biosensor enabled assays of water samples initially attributed to pure samples (the BOD range, 0.05-5.0 mg/dm). BOD assays of water samples from various sources showed the use of the receptor element of this composition to enable the data that closely correlated with the standard method ( = 0.9990).
PubMed: 32346498
DOI: 10.1007/s13205-020-02199-0 -
Environmental Microbiology Reports Jun 2020Bacteria are known to communicate with each other through signalling molecules that regulate gene expression within the population. However, the way in which hydrophobic...
Bacteria are known to communicate with each other through signalling molecules that regulate gene expression within the population. However, the way in which hydrophobic signals are released and transmitted among bacterial population is not well understood. Recent studies show that membrane vesicles (MVs) are involved in delivering hydrophobic signals, such as in N-hexadecanoyl-l-homoserine lactone (C16-HSL) signalling in Paracoccus denitrificans Pd1222. In this study, we identified the AHLs produced in Paracoccus aminophilus JCM7686, Paracoccus aminovorans NBRC16711, Paracoccus thiocyanatus JCM20756, Paracoccus versutus JCM20754 and Paracoccus yeei ATCC BAA-599, and show that the main AHL produced in all the strains is C16-HSL. Our results show that these Paracoccus species also release MVs that carry C16-HSL, but at different proportions. Most of the strains carry C16-HSL in MVs, while in P. aminophilus JCM7686, very little C16-HSL was detected in MVs, but was found in other fractions of the supernatant. Given the utilization of a common signal, we showed that these Paracoccus species can share signals with P. denitrificans Pd1222, and examined the role of MVs in signalling. Our study provides new insights into the way in which bacteria communicate using hydrophobic signals.
Topics: Acyl-Butyrolactones; Biological Transport; Extracellular Vesicles; Microbial Interactions; Paracoccus; Paracoccus denitrificans; Quorum Sensing
PubMed: 32291967
DOI: 10.1111/1758-2229.12843 -
Enfermedades Infecciosas Y... Nov 2020
Topics: Bacteremia; Humans; Liver Cirrhosis; Paracoccus
PubMed: 32127236
DOI: 10.1016/j.eimc.2020.01.013