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Biofilm Jun 2024Interspecies interactions within a biofilm community influence population dynamics and community structure, which in turn may affect the bacterial stress response to...
Interspecies interactions within a biofilm community influence population dynamics and community structure, which in turn may affect the bacterial stress response to antimicrobials. This study was conducted to assess the impact of interactions between and a three-species biofilm community (comprising , , and ) on biofilm mass, the abundance of individual species, and their survival under a laboratory-scale cleaning and disinfection (C&D) regime. The presence of enhanced the cell numbers of all three species in pairwise interactions. The outcomes derived from summing up pairwise interactions did not accurately predict the bacterial population dynamics within communities of more than two species. In four-species biofilms, we observed the dominance of and , alongside a concurrent reduction in the cell counts of and . This pattern suggests that the underlying interactions are not purely non-transitive; instead, a more complex interplay results in the dominance of specific species. We observed that bacterial spatial organization and matrix production in different mixed-species combinations affected survival in response to C&D. Confocal microscopy analysis of spatial organization showed that localized on the biofilm formed by and , and was more susceptible to C&D. Matrix production in , evidenced by alterations in biofilm mass and by scanning electron microscopy, demonstrated its protective role against C&D, not only for this species itself, but also for neighbouring species. Our findings emphasise that various social interactions within a biofilm community not only affect bacterial population dynamics but also influence the biofilm community's response to C&D stress.
PubMed: 38639000
DOI: 10.1016/j.bioflm.2024.100195 -
Microbiology Resource Announcements Apr 2024Phage Damascus was isolated from soil in northwestern Wisconsin using as the host. The Damascus genome is 56,477 bp with 3' single-stranded overhangs and 56.5% G+C...
Phage Damascus was isolated from soil in northwestern Wisconsin using as the host. The Damascus genome is 56,477 bp with 3' single-stranded overhangs and 56.5% G+C content. Damascus was assigned to cluster EL and shares 42.6%-91.7% gene content with the three other phages in this cluster.
PubMed: 38624212
DOI: 10.1128/mra.01287-23 -
Microbiology Resource Announcements Apr 2024Nucci, MCubed, and QMacho are microbacteriophages that were isolated from soil samples in Charlotte, NC. They were classified into EA10, EA2, and EB clusters,...
Nucci, MCubed, and QMacho are microbacteriophages that were isolated from soil samples in Charlotte, NC. They were classified into EA10, EA2, and EB clusters, respectively. Nucci and MCubed each had 63 predicted genes, while QMacho had 73 predicted genes.
PubMed: 38597796
DOI: 10.1128/mra.00203-24 -
EFSA Journal. European Food Safety... Apr 2024This assessment addresses a food enzyme preparation consisting of the immobilised non-viable cells of the non-genetically modified bacterium identified by the applicant...
This assessment addresses a food enzyme preparation consisting of the immobilised non-viable cells of the non-genetically modified bacterium identified by the applicant (Samyang Corporation) as strain SYG27B. This strain produces the enzyme D-psicose 3-epimerase (EC 5.1.3.30). The food enzyme preparation is used for the isomerisation of fructose to produce the speciality carbohydrate D-allulose (synonym D-psicose). Since the hazard identification and characterisation could not be made and the identity of the production organism could not be established, the Panel was unable to complete the assessment of this food enzyme preparation containing D-psicose 3-epimerase.
PubMed: 38591024
DOI: 10.2903/j.efsa.2024.8702 -
Frontiers in Plant Science 2024The strains of the genus , with more than 150 species, inhabit diverse environments; plant-associated bacteria reveal their plant growth-promoting activities due to a...
The strains of the genus , with more than 150 species, inhabit diverse environments; plant-associated bacteria reveal their plant growth-promoting activities due to a number of beneficial characteristics. Through the performance of diverse techniques and methods, including isolation of a novel strain from the aerial roots of leafless epiphytic orchid, Seidenf., its morphological and biochemical characterization, chemotaxonomy, phylogenetic and genome analysis, as well as bioassays and estimation of its auxin production capacity, a novel strain of ET2 is described. Despite that it shared 16S rRNA gene sequence similarity of 99.79% with JXJ CY 27-2, so they formed a monophyletic group on phylogenetic trees, the two strains showed clear divergence of their genome sequences. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values of ET2 differed greatly from phylogenetically close JXJ CY 27-2. Based on the differences being below the threshold for species similarity, together with the unique chemotaxonomic characteristics, strain ET2 represents a novel species of the genus . Several genes, putatively involved in auxin biosynthesis were predicted. This strain revealed obvious plant growth-promoting activities, including diazotrophy and biosynthesis of tryptophan-dependent auxins (indole-3-acetic and indole-3-pyruvic acids). Microbial auxins directly stimulated the rhizogenesis, so that the ET2-inoculated seeds of wheat, cucumber and garden cress showed evident promotion in their growth and development, both under optimal and under cold stress conditions. Based on phenotypic, chemotypic and genotypic evidences, the strain ET2 belongs to the genus , order Micrococcales, class Actinomycetes, and it represents a novel species, for which the name sp. nov. is proposed, with strain ET2 (VKPM Ac-2212, VKM Ас-2998) as the type strain.
PubMed: 38559760
DOI: 10.3389/fpls.2024.1360828 -
Heliyon Mar 2024In water-stressed regions, treated acid mine drainage (AMD) water for irrigated agriculture is a potential solution to address freshwater scarcity. However, a...
Unlocking water potential in drylands: Quicklime and fly ash enhance soil microbiome structure, ecological networks and function in acid mine drainage water-irrigated agriculture.
In water-stressed regions, treated acid mine drainage (AMD) water for irrigated agriculture is a potential solution to address freshwater scarcity. However, a significant knowledge gap exists on the short and long-term effects of treated AMD water on soil health. This study used high-throughput Illumina sequencing and predictive metagenomic profiling to investigate the impact of untreated AMD (AMD), quicklime- (A1Q and A2Q) and quicklime and fly ash-treated AMD water (AFQ) irrigation on soil bacterial diversity, co-occurrence networks and function. Results showed that untreated AMD water significantly increased soil acidity, electrical conductivity (EC), sulfate (SO), and heavy metals (HM), including reduced microbial diversity, disrupted interaction networks, and functional capacity. pH, EC, Cu, and Pb were identified as key environmental factors shaping soil microbial diversity and structure. Predominantly, , , KB913035, , and , known for their adaptability to acidic conditions and metal resistance, were abundant in AMD soils. However, soils irrigated with treated AMD water exhibited significantly reduced acidity (pH > 6.5), HM and SO levels, with an enrichment of a balanced bacterial taxa associated with diverse functions related to soil health and agricultural productivity. These taxa included , , , , , , , , , and Moreover, treated AMD water contributed to higher connectivity and balance within soil bacterial co-occurrence networks compared to untreated AMD water. These results show that quicklime/fly ash treatments can help lessen impacts of AMD water on soil microbiome and health, suggesting its potential for irrigated agriculture in water-scarce regions.
PubMed: 38533070
DOI: 10.1016/j.heliyon.2024.e27985 -
Frontiers in Cellular and Infection... 2024Gallstones are crystalline deposits in the gallbladder that are traditionally classified as cholesterol, pigment, or mixed stones based on their composition. Microbiota...
Gallstones are crystalline deposits in the gallbladder that are traditionally classified as cholesterol, pigment, or mixed stones based on their composition. Microbiota and host metabolism variances among the different types of gallstones remain largely unclear. Here, the bile and gallstone microbial species spectra of 29 subjects with gallstone disease (GSD, 24 cholesterol and 5 pigment) were revealed by type IIB restriction site-associated DNA microbiome sequencing (2bRAD-M). Among them (21 subjects: 18 cholesterol and 3 pigment), plasma samples were subjected to liquid chromatography-mass spectrometry (LC-MS) untargeted metabolomics. The microbiome yielded 896 species comprising 882 bacteria, 13 fungi, and 1 archaeon. Microbial profiling revealed significant enrichment of and sp005774735 in gallstone and and sp013044135 in the bile of cholesterol GSD subjects. The metabolome revealed 2296 metabolites, in which malvidin 3-(6''-malonylglucoside), 2-Methylpropyl glucosinolate, and ergothioneine were markedly enriched in cholesterol GSD subjects. Metabolite set enrichment analysis (MSEA) demonstrated enriched bile acids biosynthesis in individuals with cholesterol GSD. Overall, the multi-omics analysis revealed that microbiota and host metabolism interaction perturbations differ depending on the disease type. Perturbed gallstone type-related microbiota may contribute to unbalanced bile acids metabolism in the gallbladder and host, representing a potential early diagnostic marker and therapeutic target for GSD.
Topics: Humans; Gallstones; Bile Acids and Salts; Bile; Cholesterol
PubMed: 38529471
DOI: 10.3389/fcimb.2024.1283737 -
BMC Infectious Diseases Mar 2024Ralstonia pickettii is a low virulent, gram-negative bacillus that is rarely associated with human infections and may cause bacteremia. Microbacterium species are...
BACKGROUND
Ralstonia pickettii is a low virulent, gram-negative bacillus that is rarely associated with human infections and may cause bacteremia. Microbacterium species are gram-positive coryneforms that are generally considered as a contaminant in Gram staining of blood cultures, especially when the time to positivity is longer than 48 h. Both these bacterial species are emerging opportunistic pathogens that may occasionally cause serious infections and even life-threatening health conditions.
CASE PRESENTATION
Here, we report the case of a patient with bacteremia caused by both R. pickettii and Microbacterium. We advocate for providers to order rapid antibiotic susceptibility testing, since our patient's suffered two kinds of rare pathogens with the opposite of drug sensitivity results to imipenem.
CONCLUSIONS
Our case present a patient suffered septic shock caused by R. pickettii and Microbacterium. Improving the antibiotic management based on the result of antimicrobial susceptibility tests is the key of successful treatment.
Topics: Humans; Ralstonia pickettii; Microbacterium; Gram-Negative Bacterial Infections; Anti-Bacterial Agents; Bacteremia
PubMed: 38500029
DOI: 10.1186/s12879-024-09228-w -
Ecotoxicology and Environmental Safety Apr 2024Plastic pollution is a common concern of global environmental pollution. Polystyrene (PS) and polyethylene (PE) account for almost one-third of global plastic...
Plastic pollution is a common concern of global environmental pollution. Polystyrene (PS) and polyethylene (PE) account for almost one-third of global plastic production. However, so far, there have been few reports on microbial strains capable of simultaneously degrading PS and PE. In this study, Microbacterium esteraromaticum SW3, a non-pathogenic microorganism that can use PS or PE as the only carbon source in the mineral salt medium (MM), was isolated from plastics-contaminated soil and identified. The optimal growth conditions for SW3 in MM were 2% (w/v) PS or 2% (w/v) PE, 35°C and pH 6.3. A large number of bacteria and obvious damaged areas were observed on the surface of PS and PE products after inoculated with SW3 for 21 d. The degradation rates of PS and PE by SW3 (21d) were 13.17% and 5.39%, respectively. Manganese peroxidase and lipase were involved in PS and PE degradation by SW3. Through Fourier infrared spectroscopy detection, different functional groups such as carbonyl, hydroxyl and amidogen groups were produced during the degradation of PS and PE by SW3. Moreover, PS and PE were degraded into alkanes, ketones, carboxylic acids, esters and so on detected by GC-MS. Collectively, we have isolated and identified SW3, which can use PS or PE as the only carbon source in MM as well as degrade PS and PE products. This study not only provides a competitive candidate strain with broad biodegradability for the biodegradation of PS and/or PE pollution, but also provides new insights for the study of plastic biodegradation pathways.
Topics: Polystyrenes; Polyethylene; Soil; Actinomycetales; Biodegradation, Environmental; Carbon; Plastics; Microbacterium
PubMed: 38492484
DOI: 10.1016/j.ecoenv.2024.116207 -
Microbiology Resource Announcements Apr 2024Seven bacteriophages were isolated from soil in Pennsylvania and Wisconsin using the host . These bacteriophages range in the number of predicted genes encoded, from 25...
Seven bacteriophages were isolated from soil in Pennsylvania and Wisconsin using the host . These bacteriophages range in the number of predicted genes encoded, from 25 to 91, and are distributed across actinobacteriophage clusters EB, EC, EE, and EK.
PubMed: 38451225
DOI: 10.1128/mra.01251-23