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Clinical Infectious Diseases : An... May 1994Nongroupable meningococci or bacteria related to the genus Neisseria rarely cause meningitis. Complement deficiency has been identified as a major predisposing factor...
Nongroupable meningococci or bacteria related to the genus Neisseria rarely cause meningitis. Complement deficiency has been identified as a major predisposing factor for meningococcal disease. To assess whether patients with meningitis due to such strains have a complement deficiency, we studied 12 persons. Six patients had meningitis due to nongroupable strains of meningococci, and six patients had meningitis due to Moraxella species or Acinetobacter species. Inherited complement component C7 or C8 deficiency was found in two persons who had had meningitis due to nongroupable meningococci, and one C8-deficient person had had meningitis caused by Moraxella osloensis. Hypocomplementemia resulting from CSF drain-associated shunt nephritis was found in one person with meningitis due to Moraxella nonliquefaciens and in one person with meningitis due to Acinetobacter lwoffi. This rather high frequency of inherited or acquired complement deficiencies among patients with meningitis due to nongroupable meningococci, Moraxella species, and Acinetobacter species justifies the recommendation that such patients must be studied for complement deficiency.
Topics: Acinetobacter Infections; Adolescent; Adult; Cerebrospinal Fluid; Cerebrospinal Fluid Shunts; Child; Child, Preschool; Complement System Proteins; Disease Susceptibility; Female; Humans; Infant; Lupus Nephritis; Male; Meningitis, Bacterial; Meningitis, Meningococcal; Moraxella; Neisseria meningitidis; Neisseriaceae Infections; Netherlands
PubMed: 8075270
DOI: 10.1093/clinids/18.5.780 -
Revue Du Rhumatisme (Ed. Francaise :... 1994
Topics: Adult; Discitis; Female; Humans; Lumbar Vertebrae; Moraxella; Neisseriaceae Infections
PubMed: 7833897
DOI: No ID Found -
Clinical Infectious Diseases : An... Oct 1993Endophthalmitis due to gram-negative bacilli has been associated with a high degree of vision loss. We report three cases due to the nonenteric gram-negative bacilli... (Review)
Review
Endophthalmitis due to gram-negative bacilli has been associated with a high degree of vision loss. We report three cases due to the nonenteric gram-negative bacilli Moraxella nonliquefaciens, Haemophilus paraphrophilus, and multidrug-resistant Haemophilus influenzae. The features of these cases are compared with those of other reported cases of endophthalmitis due to unusual nonenteric gram-negative bacilli. Fifty-eight percent of patients had no vision in the affected eye after treatment. Early surgical intervention with vitrectomy and intravitreous antibiotics in addition to parenteral antibiotics should be included in the treatment of endophthalmitis due to gram-negative bacilli.
Topics: Actinobacillus Infections; Adult; Aged; Aggregatibacter actinomycetemcomitans; Ampicillin; Ceftriaxone; Endophthalmitis; Female; Gentamicins; Haemophilus Infections; Haemophilus influenzae; Humans; Male; Middle Aged; Moraxella; Neisseriaceae Infections; Postoperative Complications; Vitrectomy
PubMed: 8268350
DOI: 10.1093/clinids/17.4.686 -
Infection 1993Only little is known about soft tissue, bone or joint infections caused by Moraxella spp. A case of panaritium ossale et articulare caused by Moraxella nonliquefaciens...
Only little is known about soft tissue, bone or joint infections caused by Moraxella spp. A case of panaritium ossale et articulare caused by Moraxella nonliquefaciens in an 80-year-old patient immunocompromised by diabetes mellitus and liver insufficiency is reported here. Surgery, local antiseptic treatment, and therapy with aminopenicillins plus beta-lactamase-inhibitors led to complete cure of the infection within 10 days.
Topics: Aged; Aged, 80 and over; Bone Diseases; Female; Fingers; Humans; Moraxella; Neisseriaceae Infections
PubMed: 8300255
DOI: 10.1007/BF01712460 -
Carbohydrate Research Jul 1993A capsular polysaccharide, isolated from the mucoid Moraxella nonliquefaciens strain 3828/60, has been investigated by component analyses, periodate oxidation,...
A polysaccharide produced by a mucoid strain of Moraxella nonliquefaciens with a 2-acetamido-2-deoxy-5-O-(3-deoxy-beta-D-manno-octulopyranosyl)-beta-D- galactopyranosyl repeating unit.
A capsular polysaccharide, isolated from the mucoid Moraxella nonliquefaciens strain 3828/60, has been investigated by component analyses, periodate oxidation, methylation analyses, mass spectrometry, 1H and 13C NMR spectroscopy, and hydrolysis to give a disaccharide that was isolated and characterised. The results showed that the polysaccharide has the repeating unit-->3)-beta-D- GalpNAc-(1-->5)-beta-Kdo p-(2-->, with approximately 40% of O-8 of Kdo being acetylated.
Topics: Carbohydrate Conformation; Carbohydrate Sequence; Disaccharides; Indicators and Reagents; Magnetic Resonance Spectroscopy; Mass Spectrometry; Methylation; Molecular Sequence Data; Moraxella; Polysaccharides, Bacterial
PubMed: 8358744
DOI: 10.1016/0008-6215(93)80065-m -
American Journal of Ophthalmology May 1993
Topics: Aged; Anti-Bacterial Agents; Endophthalmitis; Eye Infections, Bacterial; Female; Glucocorticoids; Humans; Moraxella; Neisseriaceae Infections; Vitreous Body; beta-Lactamases
PubMed: 8488926
DOI: 10.1016/s0002-9394(14)71473-4 -
Infection and Immunity May 1993Eikenella corrodens normally inhabits the human respiratory and gastrointestinal tracts but is frequently the cause of abscesses at various sites. Using the N-terminal... (Comparative Study)
Comparative Study
Eikenella corrodens normally inhabits the human respiratory and gastrointestinal tracts but is frequently the cause of abscesses at various sites. Using the N-terminal portion of the Moraxella nonliquefaciens pilin gene as a hybridization probe, we cloned two tandemly located pilin genes of E. corrodens 31745, ecpC and ecpD, and expressed the two pilin genes separately in Escherichia coli. A comparison of the predicted amino acid sequences of E. corrodens 31745 EcpC and EcpD revealed considerable divergence between the sequences of these two pilins and even less similarity to EcpA and EcpB of E. corrodens type strain ATCC 23834. EcpC from E. corrodens 31745 displayed high degrees of homology to the pilins of Neisseria gonorrhoeae and Pseudomonas aeruginosa. EcpD from E. corrodens 31745 showed the highest homologies with the pilin of one of the three P. aeruginosa classes, whereas EcpA and EcpB of strain ATCC 23834 most closely resemble Moraxella bovis pilins. These findings raise interesting questions about potential genetic transfer between different bacterial species, as opposed to convergent evolution.
Topics: Amino Acid Sequence; Bacterial Adhesion; Bacterial Outer Membrane Proteins; Base Sequence; Blotting, Southern; Cloning, Molecular; Eikenella corrodens; Fimbriae Proteins; Genes, Bacterial; Microscopy, Electron; Molecular Sequence Data; Oligodeoxyribonucleotides; Restriction Mapping; Sequence Homology, Amino Acid
PubMed: 8478080
DOI: 10.1128/iai.61.5.1909-1916.1993 -
International Journal of Systematic... Apr 1993DNA-DNA hybridization was used to determine the levels of genomic relatedness of the three species of "false neisseriae," Neisseria caviae, Neisseria cuniculi, and... (Comparative Study)
Comparative Study
DNA-DNA hybridization was used to determine the levels of genomic relatedness of the three species of "false neisseriae," Neisseria caviae, Neisseria cuniculi, and Neisseria ovis. The reference strains of these species exhibited high levels of intraspecies relatedness (93 to 100% for N. caviae, 79 to 100% for N. cuniculi, and 68 to 100% for N. ovis) but low levels of interspecific relatedness (less than 34%) to each other and to various species belonging to the beta subclass of the Proteobacteria (Kingella kingae, Neisseria gonorrhoeae, Neisseria meningitidis, and Oligella urethralis) or to the gamma subclass (Branhamella catarrhalis, Kingella indologenes, Moraxella atlantae, Moraxella bovis, Moraxella lacunata subsp. lacunata, Moraxella lacunata subsp. liquefaciens, Moraxella nonliquefaciens, Moraxella osloensis, and Moraxella phenylpyruvica). However, the levels of DNA-DNA hybridization for the three species of "false neisseriae" were significantly higher with the species belonging to the gamma subclass (average, 13.7%) than with the species belonging to the beta subclass (average, 4.5%). These data suggest that N. caviae, N. cuniculi, and N. ovis are three separate genomic species in the gamma subclass. An ascendant hierarchical classification based only on fatty acid profiles distinguished four main classes containing (i) most of the "classical moraxellae," the "false neisseriae," and B. catarrhalis, (ii) only Acinetobacter spp., (iii) M. nonliquefaciens and "misnamed moraxellae" (M. atlantae, M. osloensis, and M. phenylpyruvica), and (iv) the "true neisseriae," the three Kingella species, and O. urethralis. Fatty acids that distinguish these four classes were identified. The fatty acid profiles of the two strains of Psychrobacter immobilis which we studied are not very similar to the profiles of the other taxa. Our results support the hypothesis that the three species of "false neisseriae," B. catarrhalis, the "classical moraxellae," and Acinetobacter spp. should be included in the same family.
Topics: Bacterial Typing Techniques; DNA, Bacterial; Fatty Acids; Humans; Neisseria; Nose; Nucleic Acid Hybridization; Phenotype; Sputum; Terminology as Topic
PubMed: 8494736
DOI: 10.1099/00207713-43-2-210 -
Zentralblatt Fur Veterinarmedizin.... Mar 1993Twenty four Moraxella related bacterias were isolated from healthy caprine and ovine nasal swabs and were investigated by classic biochemical tests and by sodium dodecyl...
Twenty four Moraxella related bacterias were isolated from healthy caprine and ovine nasal swabs and were investigated by classic biochemical tests and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins in comparison with 9 reference strains. Proteolytic and haemolytic strains were investigated by electron microscopy. The biochemical results clustered field isolates in four groups corresponding to Branhamella and Moraxella species. Proteolytic, haemolytic and fimbriated field isolates showed the same morphological structure and biochemical features as Moraxella bovis. SDS-PAGE results indicated that DICE coefficient between a field isolate and the corresponding reference strain can be as 62.5%; 41.7% and 36% respectively for the groups 1, 3 and 4. The group 2 showed a similarity percentage over 75% with the reference strain Moraxella nonliquefaciens. This results indicated that a non proteolytic but haemolytic bacteria, closely related to Moraxella nonliquefaciens was commonly isolated from small ruminants nasal flora. These animals can also be hosts of a subspecies of Moraxella bovis.
Topics: Animals; Bacterial Proteins; Goat Diseases; Goats; Moraxella; Nasal Mucosa; Neisseriaceae Infections; Sheep; Sheep Diseases
PubMed: 8322548
DOI: 10.1111/j.1439-0450.1993.tb00115.x -
The Journal of Antimicrobial... Feb 1993By employing the non-ionic detergent Triton X-100, a membrane-bound beta-lactamase was extracted from strains of Moraxella (Branhamella) catarrhalis and Moraxella...
By employing the non-ionic detergent Triton X-100, a membrane-bound beta-lactamase was extracted from strains of Moraxella (Branhamella) catarrhalis and Moraxella nonliquefaciens that produce BRO-1 and BRO-2 beta-lactamases. Unlike BRO-1 and BRO-2, which exhibit multiple major bands on isoelectric focusing (IEF), the membrane-bound enzyme focused as a single IEF band at a pI of 6.20, which was not present with either of the other two enzymes. The membrane-bound beta-lactamase could be extracted from all strains producing BRO-1 and BRO-2, including recombinant strains constructed by transformation or conjugation. The enzyme was also recovered from Escherichia coli strain HB101 carrying vector plasmid pLQ521 into which the BRO-1 beta-lactamase gene from M. catarrhalis had been cloned. All three beta-lactamases were indistinguishable by inhibitor profiles with clavulanic acid, BRL 42715, sulbactam and tazobactam. These data suggested that all three beta-lactamases were the product of a single gene in Moraxella spp., and that the membrane-bound beta-lactamase serves as a precursor of both BRO-1 and BRO-2. Species differences in cellular processing of the membrane-bound enzyme could explain the minor differences in IEF patterns that occurred with BRO-1 and BRO-2 beta-lactamases when present in different species.
Topics: Cell Membrane; Enzyme Precursors; Escherichia coli; Isoelectric Focusing; Moraxella; Moraxella catarrhalis; Octoxynol; Polyethylene Glycols; Species Specificity; beta-Lactamase Inhibitors; beta-Lactamases
PubMed: 8463169
DOI: 10.1093/jac/31.2.237